RESUMEN
We studied the effect of nanostructured clathrate complex 9-phenyl-symm-octahydoselenoxanthene (selenopyran) with ß-cyclodextrin on the generation of Ðз radicals in the Fenton system and parameters of oxidative stress in rat liver cells incubated at 37°Ð¡ for 1 h. The complex inhibits the development of free-radical oxidative processes induced by ROS and the most toxic Ðз radicals, reduces the increased level of ROS induced by prooxidants, and exhibits antioxidant activity.
Asunto(s)
Antioxidantes/farmacología , Estrés Oxidativo/efectos de los fármacos , beta-Ciclodextrinas/farmacología , Animales , Antioxidantes/síntesis química , Antioxidantes/química , Células Cultivadas , Hepatocitos/efectos de los fármacos , Hepatocitos/metabolismo , Sustancias Macromoleculares/síntesis química , Sustancias Macromoleculares/química , Sustancias Macromoleculares/farmacología , Masculino , Modelos Biológicos , Nanoestructuras/química , Piranos/síntesis química , Piranos/química , Piranos/farmacología , Ratas , Ratas Wistar , Especies Reactivas de Oxígeno/metabolismo , Compuestos de Selenio/síntesis química , Compuestos de Selenio/química , Compuestos de Selenio/farmacología , Terpenos/química , Terpenos/farmacología , beta-Ciclodextrinas/síntesis química , beta-Ciclodextrinas/químicaRESUMEN
Exposure of male Wistar rats to oxygen atmosphere at moderate pressure (1.10-1.15 atm) for 4 h resulted in significant, transient, and reversible decrease in hemolytic resistance of peripheral blood erythrocytes. In 2 days after oxygen exposure, the bone marrow released young erythrocytes with enhanced hemolytic resistance as a compensation for oxygen injury. These findings suggest that normobaric oxygen load can be viewed as a stress factor of moderate intensity.
Asunto(s)
Eritrocitos/citología , Hemólisis/fisiología , Animales , Membrana Eritrocítica/efectos de los fármacos , Membrana Eritrocítica/metabolismo , Eritrocitos/fisiología , Hemólisis/efectos de los fármacos , Masculino , Oxígeno/farmacología , Ratas , Ratas WistarRESUMEN
We studied the effect of Fe2+ ions in polymerized hemoglobin (Krunidon blood substitute) and in molecular hemoglobin (Sigma) on OH⢠radical initiation in the Fenton system. It was found that polymerized hemoglobin, as a component of Krunidon preparation, in contrast to hemoglobin tetramer, did not intensify OH⢠radical generation. The oxidant potential of Krunidon was evaluated in vivo by measuring malondialdehyde level in dog blood plasma after repeated intravenous administration (5 days in a dose of 114 mg/kg) as a biomarker. Administration of the preparation did not significantly increased malondialdehyde content on days 1 and 4 after exposure and did not affect total protein content in blood plasma. Our findings suggest that polymerized hemoglobin in the Krunidon preparation exhibits no pro-oxidant activity and can be used as the basis for the development of non-oxygenic forms of blood substitutes.
Asunto(s)
Sustitutos Sanguíneos/química , Hemoglobinas/química , Malondialdehído/sangre , Animales , Biomarcadores Farmacológicos/sangre , Sustitutos Sanguíneos/farmacocinética , Bovinos , Perros , Hemoglobinas/farmacocinética , Peróxido de Hidrógeno/química , Radical Hidroxilo/química , Hierro/química , Masculino , Oxidación-ReducciónRESUMEN
Relatively short-term (2.5 or 5 h) exposure of Wistar rats to oxygen atmosphere at moderate pressure (1.10-1.15 atm) resulted in an increase in LPO level and reduction of antioxidant activity in the blood serum. An increase in malondialdehyde concentration 1 day after termination of the exposure was followed by a decrease in the inhibiting activity of free radical oxidation of liposomal phospholipids induced by Fe(II) ions (100 µm). Malondialdehyde concentration increased by 1.29 times already after 2.5-h exposure and did not changed when the duration of the exposure to oxygen atmosphere was prolonged to 5 h. These data confirm the necessity of using substances potentiating antioxidant defense of the body during exposure to normobaric oxygenation.
