RESUMEN
The intensive use of copper (Cu) compounds as an alternative biocide in antifouling paints (APs) has resulted in wide Cu contamination into the marine environment, especially near marina harbor activities. In this work, the applicability of Cu isotopes to discriminate Cu origins related to the use of Cu-based APs in marine environments was tested. To this, Cu isotopes in APs, shipyard sludges, and sediment cores sampled in the Cu-contaminated Mediterranean marina of Port Camargue were determined. APs represent an important dominant anthropogenic source for metals in this site, making it ideal to test Cu isotopes as tracers. The overall isotope composition of four sediment cores and a surface sample varied between -0.13 and 0.44 (δ65Cu relative to NIST-976). Selected APs brands show a similar Cu concentration ~0.15 % and δ65Cu average of 0.54 ± 0.05 . The plot of δ65Cu vs concentration for all datasets allowed dissociating natural and APs end-members. However, sample isotope systematics were not consistent with a conservative mixing binary source process. Heavily Cu-contaminated sediments show isotope signatures lighter than APs brands. However, the most Cu-contaminated sample, located directly above the careening area, shows a δ65Cu slightly lighter than APs (0.44 vs 0.54 , respectively). Results suggest the preferential releasing of a heavy isotope pool by APs when these compounds are solubilized in seawater. The isotope fractionation was attributed to potential chemical Cu coordination changes during its elemental partition between paint and marina seawater and the fractionation induced by the organic ligands in the water column, before deposition. Further laboratory experiments are recommended to model the isotope fractionation mechanisms related to Cu release by APs. Because the APs' isotope signature is modified in marine environments, the use of Cu isotopes as tracers of AP in marine environments is challenging and needs more investigation.
Asunto(s)
Incrustaciones Biológicas , Desinfectantes , Contaminantes Químicos del Agua , Incrustaciones Biológicas/prevención & control , Cobre/análisis , Monitoreo del Ambiente/métodos , Sedimentos Geológicos/química , Isótopos/análisis , Pintura , Agua , Contaminantes Químicos del Agua/análisisRESUMEN
Controlled laboratory experiments were conducted to test the effects of copper (Cu2+) and butyltins (BuT) on the growth, photosynthetic activity and toxin content of two HABs (Harmful Algal Blooms) dinoflagellates, the planktonic Alexandrium catenella and the benthic Ostreopsis cf. ovata. Microalgae were exposed to increasing concentrations of Cu2+ (10-4 to 31â¯nM) or BuT (0.084 to 84â¯nM) for seven days. When considering the growth, EC50 values were 0.16 (±0.09) nM and 0.03 (±0.02) nM of Cu2+ for A. catenella and O. cf. ovata, respectively. Regarding BuT, EC50 was 14.2 (±6) nM for O. cf. ovata, while A. catenella growth inhibition appeared at BuT concentrations ≥27â¯nM. Photosynthetic activity of the studied dinoflagellates decreased with increasing Cu and BuT concentrations. For O. cf. ovata, the response of this physiological parameter to contamination was less sensitive than the biomass. Cu exposure induced the formation of temporary cysts in both organisms that could resist adverse conditions. The ovatoxin-a and -b concentrations in O. cf. ovata cells increased significantly in the presence of Cu. Altogether, the results suggest a better tolerance of the planktonic A. catenella to Cu and BuT. This could result in a differentiated selection pressure exerted by these metals on phytoplankton species in highly polluted waters. The over-production of toxins in response to Cu stress could pose supplementary health and socio-economic threats in the contaminated marine ecosystems where HABs develop.
Asunto(s)
Cobre/toxicidad , Dinoflagelados/efectos de los fármacos , Compuestos Orgánicos de Estaño/toxicidad , Fotosíntesis/efectos de los fármacos , Contaminantes Químicos del Agua/toxicidad , Biomasa , Dinoflagelados/crecimiento & desarrollo , Dinoflagelados/metabolismo , Toxinas Marinas/metabolismo , Microalgas/efectos de los fármacos , Microalgas/metabolismo , Plancton/efectos de los fármacos , Plancton/metabolismo , Contaminantes Químicos del Agua/químicaRESUMEN
The main rivers (Aude, Orb, Herault) that discharge into the Gulf of Lions and the west bank tributaries of the Rhone River including the Gardon have former non-ferrous metal mines in their upper drainage basin. Using unpublished data and data from the literature, this study provides an integrated overview of the contamination of water and sediment along the continent-sea continuum and of its impacts on the biota and on human health. In the upper part of these basins, water and stream sediments are enriched in metal(-loids) compared to median European concentrations. Arsenic is the main contaminant in the rivers Aude and Gardon d'Anduze, Sb in the Orb and Gardon d'Alès, and Tl in the Herault river. A rapid reduction in dissolved and particulate concentrations was systematically observed along the river due to dilution and precipitation. The high concentrations of metal(-loid)s observed suggest that the former mining activity still represents a potential threat for the environment, but the lack of high temporal resolution monitoring, especially during Mediterranean floods, prevents accurate assessment of metal fluxes from these rivers to the Mediterranean Sea. Studies dedicated to the impacts on human health are too rare, given that studies have shown a higher rate of arsenic-specific cancer near Salsigne mine in the Aude River basin and cases of saturnism in children in the upper Herault River basin. These studies underline the need to take environmental health issues into consideration not only in these watersheds but around the entire Mediterranean basin, which harbors numerous metalliferous ores that have been mined for millennia.
Asunto(s)
Sedimentos Geológicos/análisis , Metaloides/análisis , Metales/análisis , Minería , Ríos/química , Contaminantes Químicos del Agua/análisis , Monitoreo del Ambiente , FranciaRESUMEN
Despite the ban on tributyltin (TBT) in marine paints, harbour sediments are still highly contaminated by this antifouling agent. Concentrations of TBT and its dealkylated products dibutyltin (DBT) and monobutyltin (MBT) were determined in the pore waters of Port Camargue, a large marina located on the French Mediterranean coast. Pore waters were sampled in the field using peepers deployed in summer 2012 and 2013 and in winter 2012-2013. The winter surveys were characterized by the presence of sulphides in pore waters from a depth of 5 cm, which was not the case in winter. In summer 2013, TBT was shown to be released into pore waters below the sediment-water interface (SWI) at concentrations of up to 70 ngSn L(-1). This release was also observed in sediment anaerobic incubations and was attributed to the mineralization of the sedimentary organic matter, possible stabilization of TBT by complexation with sulphides, and lower debutylation rates in anoxic than in oxic conditions. In summer 2012, a comparatively lower concentration of TBT (around 20 ngSn L(-1) below the SWI) was measured and the presence of methyltin species was detected. We hypothesize that the differences between the two surveys reflect different microbial activity. In winter 2012-2013, marked by Fe-reducing conditions in the sediments, TBT was released into solution at the SWI at concentrations of up to 40 ngSn L(-1). Sediments are thus a continuing source of TBT for the overlying waters despite the ban on its use for boats in France.
Asunto(s)
Monitoreo del Ambiente/métodos , Sedimentos Geológicos/química , Agua de Mar/química , Contaminantes Químicos del Agua/análisis , Francia , Compuestos Orgánicos de Estaño/análisis , Pintura , Navíos , Compuestos de Trialquiltina/análisisRESUMEN
Contamination of coastal environments is often due to a complex mixture of pollutants, sometimes in trace levels, that may have significant effects on diversity and function of organisms. The aim of this study was to evaluate the short-term dynamics of bacterioplankton exposed to natural and artificial mixtures of contaminants. Bacterial communities from a southwestern Mediterranean ecosystem, lagoon and the bay (offshore) of Bizerte were exposed to i) elutriate from resuspension of contaminated sediment, and ii) an artificial mixture of metals and herbicides mimicking the contamination observed during sediment resuspension. Elutriate incubation as well as artificial spiking induced strong enrichments in nutrients (up to 18 times), metals (up to six times) and herbicides (up to 20 times) relative to the in situ concentrations in the offshore station, whereas the increases in contaminants were less marked in the lagoon station. In the offshore waters, the artificial mixture of pollutants provoked a strong inhibition of bacterial abundance, production and respiration and significant modifications of the potential functional diversity of bacterioplankton with a strong decrease of the carbohydrate utilization. In contrast, incubation with elutriate resulted in a stimulation of bacterial activities and abundances, suggesting that the toxic effects of pollutants were modified by the increase in nutrient and DOM concentrations due to the sediment resuspension. The effects of elutriate and the artificial mixture of pollutants on bacterial dynamics and the functional diversity were less marked in the lagoon waters, than in offshore waters, suggesting a relative tolerance of lagoon bacteria against contaminants.
Asunto(s)
Bacterias/efectos de los fármacos , Ecosistema , Monitoreo del Ambiente/métodos , Plancton/efectos de los fármacos , Contaminantes Químicos del Agua/toxicidad , Bacterias/crecimiento & desarrollo , Biodiversidad , Cinética , Mar Mediterráneo , Plancton/crecimiento & desarrollo , Agua de Mar/química , Agua de Mar/microbiología , Contaminantes Químicos del Agua/análisisRESUMEN
In this study, antimony (Sb) isotopic composition was determined in natural water samples collected along two hydrosystems impacted by historical mining activities: the upper Orb River and the Gardon River watershed (SE, France). Antimony isotope ratio was measured by HG-MC-ICP-MS (Hydride Generation Multi-Collector Inductively Coupled Plasma Mass Spectrometer) after a preconcentration and purification step using a new thiol-cellulose powder (TCP) procedure. The external reproducibility obtained for δ(123)Sb measurements of our in-house Sb isotopic standard solution and a certified reference freshwater was 0.06 (2σ). Significant isotopic variations were evident in surface waters from the upper Orb River (-0.06≤δ(123)Sb≤+0.11) and from the Gardon River watershed (+0.27≤δ(123)Sb≤+0.83). In particular, streams that drained different former mining sites exploited for Sb or Pb-Zn exhibited contrasted Sb isotopic signature, that may be related to various biogeochemical processes occurring during Sb transfer from rocks, mine wastes and sediments to the water compartment. Nevertheless, Sb isotopic composition appeared to be stable along the Gardon River, which might be attributed to the conservative transport of Sb at distance from mine-impacted streams, due to the relative mobile behavior of Sb(V) in natural oxic waters. This study suggests that Sb isotopic composition could be a useful tool to track pollution sources and/or biogeochemical processes in hydrologic systems.
RESUMEN
The use of symbiotic bivalve species to assess the effect of anthropogenic metal pollution was rarely investigated whereas data on filter feeding bivalves are common. The aim of this study was the exposure of two bivalve species, Ruditapes decussatus and Loripes lacteus to polymetallic pollution gradient, originating from harbor activities (Port-Camargue, south of France). Both bivalves differ by their trophic status, filter and deposit feeder for Ruditapes and symbiotic for Loripes that underlies potential differences in metal sensibility. The bivalves were immerged in July (for Ruditapes during 2 and 8 days) and in August 2012 (for Loripes during 2, 6 and 8 days) in the water column of the harbor, at 3 stations according to pollution gradient. Metal concentrations (Cu, Mn, Zn) in the water column were quantified as dissolved metals (measured by ICP-MS) and as labile metals (measured by ICP-MS using DGT technique). For each exposure time, accumulation of metals in the soft tissue of bivalves ("bioaccumulation") was measured for both species. In addition, specific parameters, according to the trophic status of each bivalve, were investigated: filtering activity (specific clearance rate, SCR) for Ruditapes, and relative cell size (SSC) and genomic content (FL1) of bacterial symbionts hosted in the gills of Loripes. The SCR of Ruditapes drops from 100% (control) to 34.7% after 2 days of exposure in the less contaminated site (station 8). On the other hand, the relative cell size (SSC) and genomic content (FL1), measured by flow cytometry were not impacted by the pollution gradient. Bioaccumulation was compared for both species, showing a greater capability of Cu accumulation for Loripes without lethal effect. Mn, Fe and Zn were generally not accumulated by any of the species according to the pollution gradient. The trophic status of each species may greatly influence their respective responses to polymetallic pollution.
Asunto(s)
Bivalvos/fisiología , Monitoreo del Ambiente , Metales/metabolismo , Contaminantes Químicos del Agua/metabolismo , Animales , Francia , Metalotioneína/metabolismo , Metales/análisis , Metales/toxicidad , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidadRESUMEN
In aquifers subject to saline water intrusion, the mixing zone between freshwater and saltwater displays strong physico-chemical gradients. Although the microbial component of these specific environments has been largely disregarded, the contribution of micro-organisms to biogeochemical reactions impacting water geochemistry has previously been conjectured. The objective of this study was to characterize and compare bacterial community diversity and composition along a vertical saline gradient in a carbonate coastal aquifer using high throughput sequencing of 16S rRNA genes. At different depths of the mixing zone, stable geochemical and hydrological conditions were associated with autochthonous bacterial communities harboring clearly distinct structures. Diversity pattern did not follow the salinity gradient, although multivariate analysis indicated that salinity was one of the major drivers of bacterial community composition, with organic carbon, pH and CO2 partial pressure. Correlation analyses between the relative abundance of bacterial taxa and geochemical parameters suggested that rare taxa may contribute to biogeochemical processes taking place at the interface between freshwater and saltwater. Bacterial respiration or alternative metabolisms such as sulfide oxidation or organic acids production may be responsible for the acidification and the resulting induced calcite dissolution observed at a specific depth of the mixing zone.
Asunto(s)
Bacterias/clasificación , Bacterias/genética , Agua Dulce/microbiología , Agua Subterránea/microbiología , Consorcios Microbianos , Agua de Mar/microbiología , Secuencia de Bases , Biodiversidad , Carbonatos/metabolismo , Genes de ARNr , Secuenciación de Nucleótidos de Alto Rendimiento , ARN Ribosómico 16S/genética , Salinidad , Análisis de Secuencia de ADN , Microbiología del AguaRESUMEN
Deciphering the biotic and abiotic factors that control microbial community structure over time and along an environmental gradient is a pivotal question in microbial ecology. Carnoulès mine (France), which is characterized by acid waters and very high concentrations of arsenic, iron, and sulfate, provides an excellent opportunity to study these factors along the pollution gradient of Reigous Creek. To this end, biodiversity and spatiotemporal distribution of bacterial communities were characterized using T-RFLP fingerprinting and high-throughput sequencing. Patterns of spatial and temporal variations in bacterial community composition linked to changes in the physicochemical conditions suggested that species-sorting processes were at work in the acid mine drainage. Arsenic, temperature, and sulfate appeared to be the most important factors that drove the composition of bacterial communities along this continuum. Time series investigation along the pollution gradient also highlighted habitat specialization for some major members of the community (Acidithiobacillus and Thiomonas), dispersal for Acidithiobacillus, and evidence of extinction/re-thriving processes for Gallionella. Finally, pyrosequencing revealed a broader phylogenetic range of taxa than previous clone library-based diversity. Overall, our findings suggest that in addition to environmental filtering processes, additional forces (dispersal, birth/death events) could operate in AMD community.
Asunto(s)
Bacterias/clasificación , Minería , Microbiología del Agua , Contaminación del Agua , Arsénico/análisis , Bacterias/genética , Bacterias/aislamiento & purificación , Biodiversidad , Hierro/análisis , Filogenia , Polimorfismo de Longitud del Fragmento de Restricción , Sulfatos/análisis , Contaminantes Químicos del Agua/análisisRESUMEN
In this study, we assessed past and present influence of ancient mining activity on metal(loid) enrichment in sediments of a former mining watershed (Gardon River, SE France), that is now industrialized and urbanized. A sedimentary archive and current sediments were characterized combining geochemical analyses, zinc isotopic analyses and sequential extractions. The archive was used to establish local geochemical background and recorded (i) increasing enrichment factors (EFs) for Pb, Zn, Cd, Tl, Hg, As and Sb throughout the industrial era, (ii) a contamination peak in 1976 attributed to a tailings dam failure, and (iii) current levels in 2002 and 2011 similar to those of 1969, except for Sb and Hg, reflecting a persisting contamination pattern. Inter-element relationships and spatial distribution of EF values of current sediments throughout the watershed suggested that both ancient and current contamination had a common origin for Pb, Zn, Cd, Tl and As related to the exploitation of Pb/Zn mineralization while old Sb mines and coal extraction area were the main sources for Sb and Hg respectively. This prevailing mining origin was reflected for Zn by a relatively uniform isotopic composition at δ(66)Zn=0.23 ± 0.03, although slight decrease from 0.23 to 0.18 was recorded from upstream to downstream sites along the river course in relation with the contribution of the lighter δ(66)Zn signature (~0.08) of acid mine drainage impacted tributaries. Results from sequential extractions revealed that the potential mobility of the studied metal(loid)s varied in the order SbAsunto(s)
Monitoreo del Ambiente
, Sedimentos Geológicos/química
, Metaloides/análisis
, Metales/análisis
, Minería
, Contaminantes Químicos del Agua/análisis
, Antimonio/análisis
, Arsénico/análisis
, Cadmio/análisis
, Francia
, Plomo/análisis
, Mercurio/análisis
, Ríos/química
, Talio/análisis
, Zinc/análisis
RESUMEN
The study of trace elements (Cu, Zn, Pb, As, Hg) and butyltin concentrations in the sediments of Port Camargue enabled assessment of the levels and history of the contamination of the largest European marina linked with the use of antifouling paints. Surface sediments near the boat maintenance area were heavily contaminated with up to 1497 µg g(-1) of Cu, 475 µg g(-1) of Zn, 0.82 µg g(-1) of Hg, 94 µg g(-1) of Pb and over 10,000 ngSn g(-1) of tributyltin (TBT). High concentrations of Hg and TBT indicate ongoing sources of these elements despite the ban on their use as biocides in paints. Sediment cores provided records of contamination since 1969. The peak concentrations of As, Hg, Pb and TBT in the sediment profile reflect their presence on boat hulls when the marina was built at the end of the 1960s. Degradation of TBT in the sediments near the boat maintenance area is slow compared to other less contaminated area of the marina.
Asunto(s)
Monitoreo del Ambiente , Sedimentos Geológicos/química , Oligoelementos/análisis , Contaminantes Químicos del Agua/análisis , Francia , Mercurio/análisis , Navíos , Compuestos de Trialquiltina/análisis , Contaminación Química del Agua/estadística & datos numéricosRESUMEN
The speciation and behaviour of antimony were investigated in surface waters downstream from the abandoned Pb-Zn Carnoulès mine (Gard, France). These waters exhibit a permanent gradient of oxygen concentration and pH, ranging from acid suboxic in Reigous Creek at the outlet of sulfide tailings impoundment, to near neutral oxygenated at downstream sites along the rivers Amous and Gardon. The concentration of total dissolved (<0.22 µm) antimony, acquired through a seven-year monitoring, decreased from 7.7-409.9 µg L(-1) at the source of Reigous Creek to 0.22-0.45 µg L(-1) in the Gardon River, showing natural Sb attenuation. Speciation analysis carried out during three surveys indicated that Sb(III) represented up to 70% of the total dissolved Sb concentration at the source of Reigous Creek, while Sb(V) represented less than 50%. Field characterization showed that Sb(III) and Sb(V) species were attenuated through dilution and were also removed from the dissolved phase during downstream transport. Speciation analysis in suspended particulate matter extracts gave a distribution of particulate Sb into 70 to 100% of Sb(III) and less than 30% of Sb(V). The removal of Sb(III) and Sb(V) species from the dissolved phase was concordant with the oversaturation of Reigous Creek water relative to Sb(III)- and Sb(V)-oxides and Sb(III)- and Sb(V)-Fe oxides. Sb(III) was more efficiently removed than Sb(V) or As(III) and it was no more detectable in the dissolved phase at downstream sites in the rivers Amous and Gardon. Conversely, the concentration of Sb(V) in the rivers Amous and Gardon still denoted contamination arising from the Carnoulès mine. The range of log Kd values, from 2.4 L kg(-1) to 4.9 L kg(-1), indicated that Sb was mainly transported in the dissolved phase downstream the Reigous Creek input. Altogether, these results give a better understanding of the fate of Sb downstream from sulfide-rich mining wastes.
Asunto(s)
Metales/análisis , Ríos/química , Contaminantes Químicos del Agua/análisis , Arsénico/análisis , Arsénico/química , Monitoreo del Ambiente , Francia , Concentración de Iones de Hidrógeno , Metales/química , Minería , Oxígeno , Contaminantes Químicos del Agua/químicaRESUMEN
A 3-year survey on sulfate-reducing bacteria (SRB) was conducted in the waters of the arsenic-rich acid mine drainage (AMD) located at Carnoulès (France) to determine the influence of environmental parameters on their community structure. The source (S5 station) exhibited most extreme conditions with pH lowering to ~1.2; iron, sulfate, and arsenic concentrations reaching 6843, 29 593, and 638 mg L(-1), respectively. The conditions were less extreme at the downstream stations S1 (pH ~3.7; iron, sulfate, and arsenic concentrations of 1114, 4207, and 167 mg L(-1), respectively) and COWG (pH ~3.4; iron, sulfate, and arsenic concentrations of 854, 3134, and 110 mg L(-1), respectively). SRB community structures were characterized by terminal restriction fragment length polymorphism and library analyses based on dsrAB genes. The predominant dsrAB sequences detected were most similar to the family Desulfobulbaceae. Additionally, certain phylotypes could be related to spatio-temporal fluctuations of pH, iron, and arsenic species. For example, Desulfohalobiaceae-related sequences were detected at the most acidic sample (pH 1.4) with high iron and arsenic concentrations (6379 and 524 mg L(-1), respectively). New dsrAB sequences, with no isolated representatives, were found exclusively in COWG. This study gives new insights on SRB community dynamics in AMD systems.
Asunto(s)
Arsénico/análisis , Minería , Bacterias Reductoras del Azufre/clasificación , Microbiología del Agua , Ácidos/análisis , ADN Bacteriano/análisis , Deltaproteobacteria/clasificación , Deltaproteobacteria/genética , Deltaproteobacteria/aislamiento & purificación , Francia , Genes Bacterianos , Concentración de Iones de Hidrógeno , Hierro/análisis , Filogenia , Polimorfismo de Longitud del Fragmento de Restricción , Estaciones del Año , Sulfatos/análisis , Bacterias Reductoras del Azufre/genética , Bacterias Reductoras del Azufre/aislamiento & purificación , Agua/químicaRESUMEN
By their metabolic activities, microorganisms have a crucial role in the biogeochemical cycles of elements. The complete understanding of these processes requires, however, the deciphering of both the structure and the function, including synecologic interactions, of microbial communities. Using a metagenomic approach, we demonstrated here that an acid mine drainage highly contaminated with arsenic is dominated by seven bacterial strains whose genomes were reconstructed. Five of them represent yet uncultivated bacteria and include two strains belonging to a novel bacterial phylum present in some similar ecosystems, and which was named 'Candidatus Fodinabacter communificans.' Metaproteomic data unravelled several microbial capabilities expressed in situ, such as iron, sulfur and arsenic oxidation that are key mechanisms in biomineralization, or organic nutrient, amino acid and vitamin metabolism involved in synthrophic associations. A statistical analysis of genomic and proteomic data and reverse transcriptase-PCR experiments allowed us to build an integrated model of the metabolic interactions that may be of prime importance in the natural attenuation of such anthropized ecosystems.
Asunto(s)
Arsénico/metabolismo , Bacterias/genética , Bacterias/metabolismo , Ecosistema , Metagenómica , Proteómica , Bacterias/clasificación , Bacterias/aislamiento & purificación , Hierro/metabolismo , Minería , Filogenia , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Azufre/metabolismoRESUMEN
Thallium concentration reached up to 534 µg L(-1) in the Reigous acid mine drainage downstream from the abandoned Pb-Zn Carnoulès mine (Southern France). It decreased to 5.44 µg L(-1) in the Amous River into which the Reigous creek flows. Tl(I) predominated (>98% of total dissolved Tl) over Tl(III), mainly in the form of Tl(+). Small amounts of Tl(III) evidenced in Reigous Creek might be in the form of aqueous TlCl(2)(+). The range of dissolved to particulate distribution coefficients log K(d) = 2.5 L kg(-1) to 4.6 L kg(-1) indicated low affinity of Tl for particles, mainly ferrihydrite, formed in the AMD-impacted watershed. The low retention of Tl(+) on ferrihydrite was demonstrated in sorption experiments, the best fit between experimental and modeled data being achieved for surface complexation constants log K(ads) = -2.67 for strong sites and log K(ads) = -3.76 for weak sites. This new set of constants allowed reasonable prediction of the concentrations of aqueous and particulate Tl resulting from the mixing of water from Reigous Creek and the Amous River water during laboratory experiments, together with those measured in the Amous River field study.
Asunto(s)
Minería , Ríos/química , Talio/análisis , Contaminantes Químicos del Agua/análisis , Contaminación Química del Agua/estadística & datos numéricos , Monitoreo del Ambiente , FranciaRESUMEN
Acid mine drainage of the Carnoulès mine (France) is characterized by acid waters containing high concentrations of arsenic and iron. In the first 30 m along the Reigous, a small creek draining the site, more than 38% of the dissolved arsenic was removed by co-precipitation with Fe(III), in agreement with previous studies, which suggest a role of microbial activities in the co-precipitation of As(III) and As(V) with Fe(III) and sulfate. To investigate how this particular ecosystem functions, the bacterial community was characterized in water and sediments by 16S rRNA encoding gene library analysis. Based on the results obtained using a metaproteomic approach on sediments combined with high-sensitivity HPLC-chip spectrometry, several GroEL orthologs expressed by the community were characterized, and the active members of the prokaryotic community inhabiting the creek sediments were identified. Many of these bacteria are ß-proteobacteria such as Gallionella and Thiomonas, but γ-proteobacteria such as Acidithiobacillus ferrooxidans and α-proteobacteria such as Acidiphilium, Actinobacteria, and Firmicutes were also detected.
Asunto(s)
Arsénico/metabolismo , Bacterias/aislamiento & purificación , Bacterias/metabolismo , Sedimentos Geológicos/microbiología , Arsénico/análisis , Bacterias/clasificación , Bacterias/genética , Biodegradación Ambiental , Sedimentos Geológicos/química , Datos de Secuencia Molecular , FilogeniaRESUMEN
A novel strictly anaerobic, moderately thermophilic, sulfate-reducing bacterium, designated strain Lam5(T), was isolated from a hot spring in north-east Tunisia and was characterized phenotypically and phylogenetically. The isolate stained Gram-negative but had a Gram-positive-type cell wall. The strain comprised endospore-forming, slightly curved rod-shaped cells with peritrichous flagella. It did not possess desulfoviridin. Strain Lam5(T) grew anaerobically at 40-60 degrees C (optimally at 55 degrees C) and at pH 5.8-8.2 (optimally at pH 7.1); it did not require NaCl but tolerated concentrations up to 1.5 % (w/v). It utilized lactate, pyruvate, formate, ethanol, butanol, glycerol, propanol and H(2) (plus acetate) as electron donors. Lactate was oxidized and pyruvate was fermented to acetate. Sulfate, sulfite, thiosulfate, As(V) and Fe(III) (but not elemental sulfur, fumarate, nitrate or nitrite) were used as electron acceptors. The G+C content of the genomic DNA was 46.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain Lam5(T) was a member of the genus Desulfotomaculum, with Desulfotomaculum putei as its closest relative (96 % similarity to the type strain). On the basis of genotypic, phenotypic and phylogenetic data, strain Lam5(T) represents a novel species of the genus Desulfotomaculum, for which the name Desulfotomaculum hydrothermale sp. nov. is proposed. The type strain is Lam5(T) (=DSM 18033(T) =JCM 13992(T)).
Asunto(s)
Desulfotomaculum/clasificación , Manantiales de Aguas Termales/microbiología , Calor , Sulfatos/metabolismo , Bacterias Reductoras del Azufre/clasificación , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/análisis , ADN Ribosómico/análisis , Desulfotomaculum/genética , Desulfotomaculum/aislamiento & purificación , Desulfotomaculum/fisiología , Genes de ARNr , Genotipo , Datos de Secuencia Molecular , Fenotipo , Filogenia , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Especificidad de la Especie , Esporas Bacterianas/fisiología , Bacterias Reductoras del Azufre/genética , Bacterias Reductoras del Azufre/aislamiento & purificación , TúnezRESUMEN
This study reports the first assessment of organotin pollution in the Hérault watershed, a medium size Mediterranean basin. Organotin compounds were analyzed in surface waters, wells supplying drinking water and sewage treatment plants (STPs). In surface and ground waters, a background contamination by total organotin compounds has been identified in the range of 0.51 +/- 0.02-71 +/- 2 ng(Sn) L(-1), which is of the same order of magnitude as those observed in other European areas. Organotins were systematically present in STP influents and sludge. Total or partial elimination of organotin compounds from treated wastewater was observed. STP effluents appeared nevertheless to be a non-negligible source of contamination not only of rivers but also of aquifers tapped for drinking water supply. Tributyltin concentration was higher than the maximum allowable concentration proposed by the European Commission in some surface waters and wells supplying drinking water. This could compromise the water resource and have serious and irreversible consequences for the aquatic eco-system. As it was the case for the ban of antifouling paints, a regulatory regime in decreasing point-source emissions of these harmful compounds used in household products might be applied.
Asunto(s)
Compuestos Orgánicos de Estaño/análisis , Ríos/química , Contaminantes Químicos del Agua/análisis , Francia , Región Mediterránea , Material Particulado/química , Aguas del Alcantarillado/químicaRESUMEN
A new thermophilic sulphate-reducing bacterium (strain Hbr5T) was enriched and isolated from a terrestrial Tunisian hot spring. It was a non-spore-forming Gram-negative curved or vibrio-shaped bacterium. It appeared singly or in long chains and was actively motile by a polar flagellum. It possessed c-type cytochromes and desulfofuscidin. Growth occurred between 50 and 70 degrees C, with an optimum of 65 degrees C at pH 7.1. In the presence of sulphate as a terminal electron acceptor, this strain readily used H2 but formate only poorly. It could use sulphate, thiosulphate, sulphite or arsenate as electron acceptors. Its DNA G+C content was 36.1 mol%. Based on phenotypic, genomic, and phylogenetic characteristics, strain Hbr5T (=DSM 18151T, =JCM 13991T) is proposed to be assigned to a novel species of genus Thermodesulfovibrio, T. hydrogeniphilus sp. nov.
Asunto(s)
Bacterias Gramnegativas/clasificación , Bacterias Gramnegativas/aislamiento & purificación , Manantiales de Aguas Termales/microbiología , Sulfatos/metabolismo , Arseniatos/metabolismo , Composición de Base , Citocromos c/análisis , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Flagelos , Formiatos/metabolismo , Genes de ARNr , Bacterias Gramnegativas/química , Bacterias Gramnegativas/genética , Calor , Hidrógeno/metabolismo , Concentración de Iones de Hidrógeno , Hidrogenosulfito Reductasa/análisis , Locomoción , Datos de Secuencia Molecular , Filogenia , ARN Bacteriano/genética , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido Nucleico , Esporas Bacterianas , Sulfitos/metabolismo , TúnezRESUMEN
Four surveys of the Huelva Estuary in southwest Spain and its sources, the Tinto and the Odiel Rivers, were carried out between 1996 and 1998. The surveys investigated the impact of metalliferous mining of sulfide-rich ores in the catchment area on metal speciation, metal concentrations in a macrophyte, and phytoplankton diversity and abundance. Chemical speciation measurements in the lower Tinto Estuary showed that metals were predominantly electrochemically labile (> 99% of total dissolved Cu, Co, and Ni at 10 microM Cu, 424 nM Co, and 500 nM Ni, S = 28). Concentrations of Cu complexing ligands and free cupric ions [Cu2+] in the Gulf of Cádiz ranged between 5.3 and 38 nM and 0.2-7.9 pM, respectively, with conditional stability constants of the ligands of log K'(CuL) = 11.7-12.6. At enhanced dissolved Cu concentrations in the lower Huelva Estuary, Cu complexing ligands were saturated with Cu, resulting in nanomolar [Cu2+], which increased upstream. Metal tissue concentrations of the macrophyte Blindingia marginata were high, and a clear relationship between dissolved labile Cu and macrophyte tissue Cu concentrations was observed. A low biodiversity was observed in the Huelva system (Shannon-Wiener indices (H) typically < 0.2). Nevertheless, the maximum biomass was observed in the lower Tinto Estuary, which showed high labile metal and nutrient concentrations and a low biodiversity (H < 0.02), thereby suggesting adaptation through evolutionary processes of the phytoplankton community to the harsh conditions.