RESUMEN
Computational methods have made substantial progress in improving the accuracy and throughput of pathology workflows for diagnostic, prognostic, and genomic prediction. Still, lack of interpretability remains a significant barrier to clinical integration. We present an approach for predicting clinically-relevant molecular phenotypes from whole-slide histopathology images using human-interpretable image features (HIFs). Our method leverages >1.6 million annotations from board-certified pathologists across >5700 samples to train deep learning models for cell and tissue classification that can exhaustively map whole-slide images at two and four micron-resolution. Cell- and tissue-type model outputs are combined into 607 HIFs that quantify specific and biologically-relevant characteristics across five cancer types. We demonstrate that these HIFs correlate with well-known markers of the tumor microenvironment and can predict diverse molecular signatures (AUROC 0.601-0.864), including expression of four immune checkpoint proteins and homologous recombination deficiency, with performance comparable to 'black-box' methods. Our HIF-based approach provides a comprehensive, quantitative, and interpretable window into the composition and spatial architecture of the tumor microenvironment.
Asunto(s)
Neoplasias/clasificación , Neoplasias/diagnóstico por imagen , Neoplasias/patología , Patología Molecular/métodos , Fenotipo , Algoritmos , Aprendizaje Profundo , Humanos , Procesamiento de Imagen Asistido por Computador , Medicina de Precisión , Microambiente TumoralRESUMEN
The quantification of changes in gene copy number is critical to our understanding of tumor biology and for the clinical management of cancer patients. DNA fluorescence in situ hybridization is the gold standard method to detect copy number alterations, but it is limited by the number of genes one can quantify simultaneously. To increase the throughput of this informative technique, a fluorescent bar-code system for the unique labeling of dozens of genes and an automated image analysis algorithm that enabled their simultaneous hybridization for the quantification of gene copy numbers were devised. We demonstrate the reliability of this multiplex approach on normal human lymphocytes, metaphase spreads of transformed cell lines, and cultured circulating tumor cells. It also opens the door to the development of gene panels for more comprehensive analysis of copy number changes in tissue, including the study of heterogeneity and of high-throughput clinical assays that could provide rapid quantification of gene copy numbers in samples with limited cellularity, such as circulating tumor cells.
Asunto(s)
Genómica , Hibridación Fluorescente in Situ/métodos , Algoritmos , Línea Celular Tumoral , Cromosomas Artificiales Bacterianos/genética , Color , Hibridación Genómica Comparativa , Colorantes Fluorescentes/química , Humanos , Sondas Moleculares/química , Reproducibilidad de los ResultadosRESUMEN
The initial steps in the synthesis of leukotrienes are the translocation of 5-lipoxygenase (5-LO) to the nuclear envelope and its subsequent association with its scaffold protein 5-lipoxygenase-activating protein (FLAP). A major gap in our understanding of this process is the knowledge of how the organization of 5-LO and FLAP on the nuclear envelope regulates leukotriene synthesis. We combined single molecule localization microscopy with Clus-DoC cluster analysis, and also a novel unbiased cluster analysis to analyze changes in the relationships between 5-LO and FLAP in response to activation of RBL-2H3 cells to generate leukotriene C4. We identified the time-dependent reorganization of both 5-LO and FLAP into higher-order assemblies or clusters in response to cell activation via the IgE receptor. Clus-DoC analysis identified a subset of these clusters with a high degree of interaction between 5-LO and FLAP that specifically correlates with the time course of LTC4 synthesis, strongly suggesting their role in the initiation of leukotriene biosynthesis.
Asunto(s)
Proteínas Activadoras de la 5-Lipooxigenasa/metabolismo , Araquidonato 5-Lipooxigenasa/metabolismo , Basófilos/metabolismo , Leucotrieno C4/biosíntesis , Membrana Nuclear/metabolismo , Proteínas Activadoras de la 5-Lipooxigenasa/química , Proteínas Activadoras de la 5-Lipooxigenasa/genética , Animales , Araquidonato 5-Lipooxigenasa/química , Araquidonato 5-Lipooxigenasa/genética , Basófilos/citología , Basófilos/efectos de los fármacos , Línea Celular Tumoral , Análisis por Conglomerados , Regulación de la Expresión Génica , Inmunoglobulina E/genética , Inmunoglobulina E/metabolismo , Inmunoglobulina E/farmacología , Membrana Nuclear/efectos de los fármacos , Membrana Nuclear/genética , Membrana Nuclear/ultraestructura , Unión Proteica , Ratas , Receptores de IgE/genética , Receptores de IgE/metabolismo , Transducción de Señal , Imagen Individual de MoléculaRESUMEN
Antibiotic resistance is compromising our ability to treat bacterial infections. Clinical microbiology laboratories guide appropriate treatment through antimicrobial susceptibility testing (AST) of patient bacterial isolates. However, increasingly, pathogens are developing resistance to a broad range of antimicrobials, requiring AST of alternative agents for which no commercially available testing methods are available. Therefore, there exists a significant AST testing gap in which current methodologies cannot adequately address the need for rapid results in the face of unpredictable susceptibility profiles. To address this gap, we developed a multicomponent, microscopy-based AST (MAST) platform capable of AST determinations after only a 2 h incubation. MAST consists of a solid-phase microwell growth surface in a 384-well plate format, inkjet printing-based application of both antimicrobials and bacteria at any desired concentrations, automated microscopic imaging of bacterial replication, and a deep learning approach for automated image classification and determination of antimicrobial minimal inhibitory concentrations (MICs). In evaluating a susceptible strain set, 95.8% were within ±1 and 99.4% were within ±2, twofold dilutions, respectively, of reference broth microdilution MIC values. Most (98.3%) of the results were in categorical agreement. We conclude that MAST offers promise for rapid, accurate, and flexible AST to help address the antimicrobial testing gap.
Asunto(s)
Antiinfecciosos/farmacología , Bacterias/efectos de los fármacos , Pruebas de Sensibilidad Microbiana/métodos , Microscopía/métodos , Humanos , Factores de TiempoRESUMEN
For a cell to move forward it must convert chemical energy into mechanical propulsion. Force produced by actin polymerization can generate traction across the plasma membrane by transmission through integrins to their ligands. However, the role this force plays in integrin activation is unknown. Here we show that integrin activity and cytoskeletal dynamics are reciprocally linked, where actin-dependent force itself appears to regulate integrin activity. We generated fluorescent tension-sensing constructs of integrin αLß2 (LFA-1) to visualize intramolecular tension during cell migration. Using quantitative imaging of migrating T cells, we correlate tension in the αL or ß2 subunit with cell and actin dynamics. We find that actin engagement produces tension within the ß2 subunit to induce and stabilize an active integrin conformational state and that this requires intact talin and kindlin motifs. This supports a general mechanism where localized actin polymerization can coordinate activation of the complex machinery required for cell migration.
Asunto(s)
Actinas/metabolismo , Antígenos CD18/metabolismo , Movimiento Celular , Linfocitos T/citología , Linfocitos T/metabolismo , Secuencias de Aminoácidos , Sitios de Unión , Fenómenos Biomecánicos , Antígenos CD18/química , Células HEK293 , Humanos , Células Jurkat , Ligandos , Modelos Biológicos , Conformación Proteica , Subunidades de Proteína/metabolismo , Resistencia a la TracciónRESUMEN
Inhibition of granule cells plays a key role in gating the flow of signals into the cerebellum, and it is thought that Golgi cells are the only interneurons that inhibit granule cells. Here we show that Purkinje cells, the sole output neurons of the cerebellar cortex, also directly inhibit granule cells via their axon collaterals. Anatomical and optogenetic studies indicate that this non-canonical feedback is region specific: it is most prominent in lobules that regulate eye movement and process vestibular information. Collaterals provide fast, slow, and tonic inhibition to granule cells, and thus allow Purkinje cells to regulate granule cell excitability on multiple timescales. We propose that this feedback mechanism could regulate excitability of the input layer, contribute to sparse coding, and mediate temporal integration.
Asunto(s)
Corteza Cerebelosa/citología , Inhibición Neural , Neuronas/fisiología , Células de Purkinje/fisiología , Animales , Corteza Cerebelosa/metabolismo , Ratones , Sinapsis/fisiología , Ácido gamma-Aminobutírico/metabolismoRESUMEN
To achieve accurate spatiotemporal patterns of gene expression, RNA-binding proteins (RBPs) guide nuclear processing, intracellular trafficking and local translation of target mRNAs. In neurons, RBPs direct transport of target mRNAs to sites of translation in remote axons and dendrites. However, it is not known whether an individual RBP coordinately regulates multiple mRNAs within these morphologically complex cells. Here we identify SFPQ (splicing factor, poly-glutamine rich) as an RBP that binds and regulates multiple mRNAs in dorsal root ganglion sensory neurons and thereby promotes neurotrophin-dependent axonal viability. SFPQ acts in nuclei, cytoplasm and axons to regulate functionally related mRNAs essential for axon survival. Notably, SFPQ is required for coassembly of LaminB2 (Lmnb2) and Bclw (Bcl2l2) mRNAs in RNA granules and for axonal trafficking of these mRNAs. Together these data demonstrate that SFPQ orchestrates spatial gene expression of a newly identified RNA regulon essential for axonal viability.
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Axones/fisiología , Factor de Empalme Asociado a PTB/fisiología , ARN/metabolismo , Regulón/fisiología , Animales , Proteínas Reguladoras de la Apoptosis , Transporte Axonal/fisiología , Supervivencia Celular/fisiología , Ganglios Espinales/metabolismo , Técnicas de Silenciamiento del Gen , Lamina Tipo B/metabolismo , Ratones , Ratones Noqueados , Factor de Empalme Asociado a PTB/genética , Proteínas/genética , Proteínas/metabolismo , Células Receptoras Sensoriales/metabolismoRESUMEN
Swarming represents a special case of bacterial behavior where motile bacteria migrate rapidly and collectively on surfaces. Swarming and swimming motility of bacteria has been studied well for rigid, self-propelled rods. In this study we report a strain of Vibrio alginolyticus, a species that exhibits similar collective motility but a fundamentally different cell morphology with highly flexible snake-like swarming cells. Investigating swarming dynamics requires high-resolution imaging of single cells with coverage over a large area: thousands of square microns. Researchers previously have employed various methods of motion analysis but largely for rod-like bacteria. We employ temporal variance analysis of a short time-lapse microscopic image series to capture the motion dynamics of swarming Vibrio alginolyticus at cellular resolution over hundreds of microns. Temporal variance is a simple and broadly applicable method for analyzing bacterial swarming behavior in two and three dimensions with both high-resolution and wide-spatial coverage. This study provides detailed insights into the swarming architecture and dynamics of Vibrio alginolyticus isolate B522 on carrageenan agar that may lay the foundation for swarming studies of snake-like, nonrod-shaped motile cell types.
Asunto(s)
Movimiento , Vibrio alginolyticus/fisiología , Materiales Biomiméticos/metabolismo , Flagelos/metabolismo , Algas Marinas/metabolismo , Vibrio alginolyticus/metabolismoRESUMEN
Highly redundant pathways often contain components whose functions are difficult to decipher from the responses induced by genetic or molecular perturbations. Here, we present a statistical approach that samples and registers events observed in images of intrinsic fluctuations in unperturbed cells to establish the functional hierarchy of events in systems with redundant pathways. We apply this approach to study the recruitment of actin assembly factors involved in the protrusion of the cell membrane. We find that the formin mDia1, along with nascent adhesion components, is recruited to the leading edge of the cell before protrusion onset, initiating linear growth of the lamellipodial network. Recruitment of Arp2/3, VASP, cofilin, and the formin mDia2 then promotes sustained exponential growth of the network. Experiments changing membrane tension suggest that Arp2/3 recruitment is mechano-responsive. These results indicate that cells adjust the overlapping contributions of multiple factors to actin filament assembly during protrusion on a ten-second timescale and in response to mechanical cues.
RESUMEN
Eukaryotes have evolved multiple strategies for maintaining cellular protein homeostasis. One such mechanism involves neutralization of deleterious protein aggregates via their defined spatial segregation. Here, using the molecular disaggregase Hsp104 as a marker for protein aggregation, we describe the spatial and temporal dynamics of protein aggregates in the filamentous fungus Aspergillus nidulans. Filamentous fungi, such as A. nidulans, are a diverse group of species of major health and economic importance and also serve as model systems for studying highly polarized eukaryotic cells. We find that microtubules promote the formation of Hsp104-positive aggregates, which coalesce into discrete subcellular structures in a process dependent on the microtubule-based motor cytoplasmic dynein. Finally, we find that impaired clearance of these inclusions negatively impacts retrograde trafficking of endosomes, a conventional dynein cargo, indicating that microtubule-based transport can be overwhelmed by chronic cellular stress.
Asunto(s)
Dineínas Citoplasmáticas/genética , Proteínas Fúngicas/genética , Proteínas de Choque Térmico/genética , Proteínas Asociadas a Microtúbulos/genética , Agregado de Proteínas/genética , Aspergillus nidulans/genética , Transporte Biológico , Dineínas Citoplasmáticas/metabolismo , Endosomas/genética , Endosomas/metabolismo , Proteínas de Choque Térmico/metabolismo , Proteínas Asociadas a Microtúbulos/metabolismo , Microtúbulos/metabolismoRESUMEN
The basal ganglia are phylogenetically conserved subcortical nuclei necessary for coordinated motor action and reward learning. Current models postulate that the basal ganglia modulate cerebral cortex indirectly via an inhibitory output to thalamus, bidirectionally controlled by direct- and indirect-pathway striatal projection neurons (dSPNs and iSPNs, respectively). The basal ganglia thalamic output sculpts cortical activity by interacting with signals from sensory and motor systems. Here we describe a direct projection from the globus pallidus externus (GP), a central nucleus of the basal ganglia, to frontal regions of the cerebral cortex (FC). Two cell types make up the GP-FC projection, distinguished by their electrophysiological properties, cortical projections and expression of choline acetyltransferase (ChAT), a synthetic enzyme for the neurotransmitter acetylcholine (ACh). Despite these differences, ChAT(+) cells, which have been historically identified as an extension of the nucleus basalis, as well as ChAT(-) cells, release the inhibitory neurotransmitter GABA (γ-aminobutyric acid) and are inhibited by iSPNs and dSPNs of dorsal striatum. Thus, GP-FC cells comprise a direct GABAergic/cholinergic projection under the control of striatum that activates frontal cortex in vivo. Furthermore, iSPN inhibition of GP-FC cells is sensitive to dopamine 2 receptor signalling, revealing a pathway by which drugs that target dopamine receptors for the treatment of neuropsychiatric disorders can act in the basal ganglia to modulate frontal cortices.
Asunto(s)
Lóbulo Frontal/metabolismo , Globo Pálido/metabolismo , Ácido gamma-Aminobutírico/metabolismo , Acetilcolina/metabolismo , Animales , Antipsicóticos/farmacología , Núcleo Basal de Meynert/citología , Núcleo Basal de Meynert/metabolismo , Colina O-Acetiltransferasa/metabolismo , Fenómenos Electrofisiológicos , Femenino , Lóbulo Frontal/citología , Lóbulo Frontal/efectos de los fármacos , Globo Pálido/citología , Globo Pálido/efectos de los fármacos , Globo Pálido/enzimología , Macaca mulatta , Masculino , Ratones , Vías Nerviosas , Receptores de Dopamina D2/metabolismo , Transducción de SeñalRESUMEN
Residential abandonment is on the rise in many urban areas, with unknown implications for ecosystem structure and function on land slated for partial or full restoration to native habitat. Partial decoupling of human and natural systems could reduce disturbance (e.g., trampling, recreational traffic) and modify vegetation structure in a way that alters soil carbon storage, an ecosystem function that many municipalities consider a management objective of growing importance. We quantified soil carbon percent and mass to 10 cm depth and examined vegetation structure in 50 vacant and 10 occupied residential lawns located in Richmond, VA, with the principal objective of determining whether occupancy status alters trajectories of soil carbon storage or its correspondence with household economic/demographic indicators and vegetation cover. Abandoned residential lawns supported significantly less grass cover, but these declines were largely offset by increases in emergent overstory (>1 m height) vegetation cover. Soil carbon percent and mass did not differ between lawns of occupied and abandoned residences, even though significant, but highly uncertain, increases in soil carbon mass occurred in the first decade following vacancy. Instead, all residential lawns exhibited similar significant increases in soil carbon percent and mass with increasing residence age and neighborhood affluence, the former indicating annual carbon accretion rates of 20 g m(-2). We conclude that in this early stage of vacancy, soil carbon storage is already subtly responding to declines in human intervention, with reduced soil disturbance and sustained vegetation cover in abandoned lawns playing likely roles in emerging soil carbon storage trajectories.
Asunto(s)
Ciclo del Carbono , Carbono/análisis , Poaceae , Suelo/análisis , Humanos , Factores Socioeconómicos , VirginiaRESUMEN
Cell movement begins with a leading edge protrusion, which is stabilized by nascent adhesions and retracted by mature adhesions. The ERK-MAPK (extracellular signal-regulated kinase-mitogen-activated protein kinase) localizes to protrusions and adhesions, but how it regulates motility is not understood. We demonstrate that ERK controls protrusion initiation and protrusion speed. Lamellipodial protrusions are generated via the WRC (WAVE2 regulatory complex), which activates the Arp2/3 actin nucleator for actin assembly. The WRC must be phosphorylated to be activated, but the sites and kinases that regulate its intermolecular changes and membrane recruitment are unknown. We show that ERK colocalizes with the WRC at lamellipodial leading edges and directly phosphorylates two WRC components: WAVE2 and Abi1. The phosphorylations are required for functional WRC interaction with Arp2/3 and actin during cell protrusion. Thus, ERK coordinates adhesion disassembly with WRC activation and actin polymerization to promote productive leading edge advancement during cell migration.
Asunto(s)
Movimiento Celular/fisiología , Quinasas MAP Reguladas por Señal Extracelular/metabolismo , Seudópodos/metabolismo , Familia de Proteínas del Síndrome de Wiskott-Aldrich/metabolismo , Complejo 2-3 Proteico Relacionado con la Actina/genética , Complejo 2-3 Proteico Relacionado con la Actina/metabolismo , Actinas/genética , Actinas/metabolismo , Proteínas Adaptadoras Transductoras de Señales/genética , Proteínas Adaptadoras Transductoras de Señales/metabolismo , Células Cultivadas , Proteínas del Citoesqueleto/genética , Proteínas del Citoesqueleto/metabolismo , Células Epiteliales/citología , Células Epiteliales/fisiología , Quinasas MAP Reguladas por Señal Extracelular/genética , Humanos , Fosforilación , Familia de Proteínas del Síndrome de Wiskott-Aldrich/genéticaRESUMEN
During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores, commonly referred to as breathing. We developed a live cell imaging assay combined with computational image analysis to quantify the properties and dynamics of sister kinetochores in three dimensions. We show that baseline oscillation and breathing speeds in late prometaphase and metaphase are set by microtubule depolymerases, whereas oscillation and breathing periods depend on the stiffness of the mechanical linkage between sisters. Metaphase plates become thinner as cells progress toward anaphase as a result of reduced oscillation speed at a relatively constant oscillation period. The progressive slowdown of oscillation speed and its coupling to plate thickness depend nonlinearly on the stiffness of the mechanical linkage between sisters. We propose that metaphase plate formation and thinning require tight control of the state of the mechanical linkage between sisters mediated by centromeric chromatin and cohesion.