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BACKGROUND: Immunization programs suffer recurrent setbacks in developing countries. PURPOSE: We evaluated the knowledge and opinion of parents towards childhood immunization. MATERIALS AND METHODS: A cross-sectional study was conducted among 2400 parents/guardians in two major Anambra cities. RESULTS: The male:female ratio was 1:1 and about two-third (64.3%) of respondents were aged 21-40 years. The majority were married (85.0%), Christians (88.3%), and had heard about childhood immunization (92.3%) mainly from formal settings (56.5%). A little above half (56.2%) of them correctly cited "disease prevention" as reason for childhood immunization. A larger proportion of those that gave this correct response worked in tertiary institutions and had post-secondary school education (p<0.001). The majority of the respondents appropriately agreed or disagreed with opinions that can influence immunization uptake. However, some of them did not agree that immunization was important during the first year of life (16.7%) or afterwards (23.1%); to ensure full immunization (22.8%) or maintain proper immunization records (25.6%) of their children; and to actively support childhood immunization (33.9%). Likewise, some respondents would withhold immunization for perceived fear of adverse reactions (30.7%) or if naturally acquired infection was perceived to confer better protection (28.2%). Respondents who worked in tertiary institutions, and had higher education or family income were more likely to agree or disagree appropriately to opinions. Males had comparable opinions with females although females seemed to do better in opinions that reflect actual vaccination practice. CONCLUSION: Awareness of the term "immunization" was high although knowledge of its indication did not measure up with this awareness, especially among the less educated. Most parents, especially those who worked in tertiary institutions,r had higher income, or education, were favorably disposed towards opinions that could positively influence immunization uptake. Efforts should be intensified at improving awareness on the indication, benefits and safety of immunization, and improving public opinions in order to optimize childhood immunization.
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Extended-spectrum ß-lactamase (ESBL)-producing organisms have become a serious challenge in healthcare delivery globally. The prevalence of ESBL carriage in healthy and sick children in Enugu, Nigeria, was bacteriologically investigated in this study. Four hundred and twenty-two biological samples (mid-stream urine and feces) were bacteriologically analyzed. The isolates were screened for ESBL production using Clinical and Laboratory Standards Institute (CLSI) breakpoints. The suspected ESBL producers were confirmed using double disc synergy test method. Out of the 162 isolates screened, 32 (19.8%) were confirmed as ESBL positive, with a prevalence of 25.32% among sick children in Enugu State University Teaching Hospital (ESUTH), Parklane, Enugu and 13.89% in apparently healthy children in a community setting. Klebsiella spp. and Escherichia coli had the highest prevalence of 34.6% and 28.6%, respectively; Citrobacter spp. and Enterobacter spp. were 18.2% and 16.7%, respectively. The ESBL positive isolates were resistant to sulfamethoxazole/trimethoprim (100%), tetracycline (100%), kanamycin (96.9%), nitrofurantoin (84.4%), ciprofloxacin (68.6%), and chloramphenicol (62.5%) but susceptible to meropenem (100%), colistin (56.3%), and gentamicin (50%). Klebsiella spp. had the highest ESBL occurrence among sick children while E. coli had the highest ESBL occurrence among healthy children in Enugu. All ESBL-positive isolates were multiply resistant to conventional antibiotics. The emergence and spread of ß-lactamase-producing Enterobacteriaceae in hospital and community environments highlight the possibility for an infection outbreak if not checked.
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BACKGROUND: Urinary Tract Infection (UTI) is a common contagion among men and women with the incidence relatively higher among women due to their differing anatomy. An understanding of the kind of pathogens implicated in urinary tract infections as well as antibiotic susceptibility profiling may help the clinician make rationally correct empirical choice in their treatment. OBJECTIVE: This study is aimed at determining the type and antibiotic susceptibility pattern of bacterial uropathogens isolated from female patients attending Chukwuemeka Odumegwu Ojukwu University Teaching Hospital (COOUTH), Awka, Nigeria. METHOD: Two hundred and forty patients with clinically diagnosed UTI and who were on at least 5 days' antibiotic holiday were recruited into the study. Their demographic characteristics were captured using pre-tested questionnaire. Their clean catch mid-stream urine samples were collected using sterile universal container and sent to the Microbiology Department for processing. Within 30 minutes of samples collection, the specimens were cultured and the isolates were identified, after 24 h of incubation, using standard microbiological techniques. Antibiotic susceptibility tests were done with standard antibiotic discs using the Kirby-bauer disc diffusion method. RESULTS: Out of the 240 urine samples, 89.17% yielded significant bacteriuria. The pathogens implicated were Escherichia coli (28.5%), Staphylococcus aureus (28.0%), Salmonella spp (22.8%) and Pseudomonas aeruginosa (20.5%). HIV status, patients age, pregnancy status and marital status all significantly affected bacteriuria rate (p value < 0.05), while patients' location (sub-urban/rural dwelling), and level of education did not (p value > 0.05). The pattern of microbial resistance to antibiotics suggests that ceftazidime, fosfomycin and cefoxitin may not be used as first-line agents in the empirical treatment of UTIs rather; levofloxacin, meropenem or aztreonam should be considered. Levofloxacin was significantly effective against all the isolates and may be administered empirically while waiting for the culture result (Mean % susceptibility was 79.85). CONCLUSION: E. coli and S. aureus were the predominant pathogens in the study and many were resistant to the commonly prescribed antibiotics and so leave the clinicians with only few alternative drugs for UTIs treatment. Routine surveillance and monitoring studies need to be constantly conducted to update clinicians on the prevalent pathogens and the rational and empirical treatment of UTIs. Aggressive and consistent health education using every possible media is also recommended to combat the menace of drug resistance occasioned by inappropriate antibiotic use.
