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Background: Although in vitro methods have disadvantages, they are still commonly used to measure nerve conduction velocity (NCV) in experimental studies. Therefore, this study was designed to demonstrate the effect of the surgical procedures required for in vitro methods on nerve fibers and the effect of in vivo and in vitro methods on the results of electrophysiological measurements.Methods: Rats were assigned to the in vivo (control-1, injury-1, and diabetic-1) and in vitro (control-2, injury-2, and diabetic-2) groups. The NCV and compound action potential amplitudes were measured, and the nerve fibers were histologically examined.Results: Damaged axons and myelin sheaths were observed in the control-2 group. The electrophysiological values of the in vitro groups were lower than those of the in vivo groups. Furthermore, these values were lower for the diabetic and injury groups than for the control groups.Conclusions: This study showed that the surgical procedures required for the in vitro method reduced the measured values. Owing to the previous and current disadvantages of the in vitro method, the in vivo method was more sensitive for the NCV measurement. Moreover, measurements can be performed using the current in vivo method for small nerve fibers.
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Axones , Conducción Nerviosa , Potenciales de Acción/fisiología , Animales , Vaina de Mielina , Conducción Nerviosa/fisiología , RatasRESUMEN
Ozone autohemotherapy is used in the treatment of some diseases. Carbonic anhydrases (CAs, EC 4.2.1.1) are metalloenzymes and play a role in homeostatic mechanisms. The aim of this study was to investigate the effects of ozone on human red blood cell CA (hCA) enzyme activity. Blood samples were treated with different doses of ozone (10, 20, 30 µg/ml) and the erythrocyte total CA activities were determined. Also, purified hCAI and hCAII isozymes were treated with the same doses of ozone and the enzyme activities were measured. About 30 µg/ml ozone treatment decreased the purified hCAI and hCAII activity and increased the total CA activity compared to the control. Because the implication of CAs on many physiological and biochemical processes is linked to pathologies, it can be suggested that the ozone at a concentration of 30 µg/ml is safely used by autohaemotherapy in a well-designed clinical trial.
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Inhibidores de Anhidrasa Carbónica/farmacología , Anhidrasas Carbónicas/metabolismo , Eritrocitos/efectos de los fármacos , Oxidantes Fotoquímicos/farmacología , Ozono/farmacología , Anhidrasa Carbónica I/antagonistas & inhibidores , Anhidrasa Carbónica I/aislamiento & purificación , Anhidrasa Carbónica I/metabolismo , Anhidrasa Carbónica II/antagonistas & inhibidores , Anhidrasa Carbónica II/aislamiento & purificación , Anhidrasa Carbónica II/metabolismo , Inhibidores de Anhidrasa Carbónica/efectos adversos , Anhidrasas Carbónicas/química , Sistema Libre de Células/efectos de los fármacos , Sistema Libre de Células/enzimología , Cromatografía de Afinidad , Eritrocitos/enzimología , Humanos , Concentración Osmolar , Oxidantes Fotoquímicos/efectos adversos , Ozono/efectos adversosRESUMEN
BACKGROUND: Accelerated apoptosis plays a vital role in the development of diabetic vascular complications. Ozone may attenuate diabetic nephropathy by means of decreased apoptosis-related genes. The aim of our study was to investigate the effect of ozone therapy on streptozotocin-induced diabetic nephropathy in rats. Also the histopathological changes in diabetic kidney tissue with ozone treatment were evaluated. METHODS: The rats were randomly divided into six groups (n = 7): control (C), ozone (O), diabetic (D), ozone-treated diabetic (DO), insulin-treated diabetic (DI), and ozone- and insulin-treated diabetic (DOI). D, DI, and DOI groups were induced by a single intraperitoneal injection of streptozotocin. Ozone was given to the O, DO, and DOI groups. Group DI and DOI received subcutaneous (SC) insulin (3 IU). All animals received daily treatment for 6 weeks. RESULTS: Expressions of caspase-1-3-9, HIF-1α, and TNF-α genes were significantly higher in D group compared to C group (p < 0.05 for all). Ozone treatment resulted in significant decrease in the expressions of these genes in diabetic kidney tissue compared to both C and D group (p < 0.05 for all). Caspase-1-3-9, HIF-1α, and TNF-α gene expressions were found to be lower in DOI group compared to C group (p < 0.05 for all). Also adding ozone treatment to insulin therapy resulted in more significantly decrease in the expressions of these genes in diabetic tissue compared to only insulin-treated diabetic group (p < 0.05 for all). Regarding histological changes, ozone treatment resulted in decrease in the renal corpuscular inflammation and normal kidney morphology was observed. Both insulin and ozone therapies apparently improved kidney histological findings with less degenerated tubules and less inflammation of renal corpuscle compared to D, DO, and DI groups. CONCLUSION: Ozone therapy decreases the expressions of apoptotic genes in diabetic kidney tissue and improves the histopathological changes.
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Caspasas/genética , Diabetes Mellitus Experimental , Nefropatías Diabéticas/genética , Regulación de la Expresión Génica , Subunidad alfa del Factor 1 Inducible por Hipoxia/genética , Ozono/uso terapéutico , Factor de Necrosis Tumoral alfa/genética , Animales , Apoptosis , Caspasas/biosíntesis , Nefropatías Diabéticas/metabolismo , Nefropatías Diabéticas/terapia , Subunidad alfa del Factor 1 Inducible por Hipoxia/biosíntesis , Etiquetado Corte-Fin in Situ , Masculino , Oxidantes Fotoquímicos/uso terapéutico , ARN Mensajero/genética , Ratas , Ratas Sprague-Dawley , Reacción en Cadena en Tiempo Real de la Polimerasa , Factor de Necrosis Tumoral alfa/biosíntesisRESUMEN
The aim of the study is to investigate the effects of the interleukin-6 (IL-6) blocker tocilizumab in a hyperstimulated rat model and compare it with ranibizumab, a gonadotropin-releasing hormone antagonist (GnRHA), and cabergoline. Forty-seven rats were randomly divided into the following seven groups: Group 1: OHS; Group 2: OHS+ GnRHA; Group 3: OHS + ranibizumab; Group 4: OHS + cabergoline; Group 5: OHS + low-dose tocilizumab (TL); Group 6: OHS + high-dose tocilizumab (TH); Group 7: sham. Ovarian weight was significantly lower only in the ranibizumab group than in the OHS group. Estrogen levels were significantly lower in the GnRHA group than in the OHS and the treatment groups. Progesterone levels were significantly lower in the ranibizumab, cabergoline, and TL groups than in the OHS group. Among the treatment groups, corpus luteum counts were lower than in the OHS group. Corpus luteum counts were lowest in the tocilizumab groups. IL-6 intensity was lower in all treatment groups than in the OHS group. In the ranibizumab group IL-6 intensity was the lowest. The TL group did not significantly differ from the GnRHA and cabergoline groups regarding IL-6 expression. Ovarian VEGF expression was significantly lower in all treatment groups. For the TL, ranibizumab, and cabergoline groups VEGF intensity was similar. Tocilizumab may be a new strategy for preventing ovarian hyperstimulation syndrome by inhibition of IL-6.
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Anticuerpos Monoclonales Humanizados/uso terapéutico , Ergolinas/uso terapéutico , Hormona Liberadora de Gonadotropina/antagonistas & inhibidores , Interleucina-6/antagonistas & inhibidores , Síndrome de Hiperestimulación Ovárica/prevención & control , Ranibizumab/uso terapéutico , Animales , Cabergolina , Estrógenos/sangre , Femenino , Interleucina-6/análisis , Síndrome de Hiperestimulación Ovárica/patología , Ovario/química , Ovario/patología , Progesterona/sangre , Ratas , Ratas Wistar , Factor A de Crecimiento Endotelial Vascular/análisis , Factor A de Crecimiento Endotelial Vascular/antagonistas & inhibidoresRESUMEN
OBJECTIVE: Pathogenesis of Henoch-Schönlein purpura (HSP) is not clearly defined. The present study was conducted to investigate the alterations in erythrocyte deformability and oxidative stress in HSP and to examine the possible relationship between erythrocyte deformability and organ involvement in this disease. METHODS: Plasma malondialdehyde (MDA) levels, total antioxidant status (TAS), erythrocyte deformability and aggregation were measured in 21 children with HSP at the disease onset and during the remission period in comparison with healthy subjects. FINDINGS: HSP patients at the active stage had significantly higher MDA and lower TAS levels (P<0.05). Erythrocyte deformability was decreased at the active-stage and increased again at the remission period of HSP (P<0.05). Erythrocyte deformability was significantly decreased at four different shear stresses in patients with gastrointestinal system or renal involvement; and decreased at six different shear stresses in patients with gastrointestinal system, and renal involvement compared to the patients without organ involvement (P<0.05). No significant difference was observed in aggregation parameters (P>0.05). CONCLUSION: The present findings emphasize the association between impaired erythrocyte deformability and organ involvement in HSP.
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Carbonic anhydrase (CA) is an enzyme which plays a role in various homeostatic mechanisms, such as acid-base balance and electrolyte secretion in a various tissues. This study was aimed at determining and comparing possible alterations in activity of this enzyme caused by the use of old (Carbamazepine, Phenytoin Sodium, Sodium Valproate) and new (Levetiracetam, Pregabalin, Gabapentin, Oxcarbazepine) anti-epileptic drugs. Blood samples were collected from the volunteers. The blood samples were centrifuged to separate plasma and erythrocyte package. Hemolysate was prepared from the red cells. CA I and II were purified from human erythrocytes by a simple one step procedure using Sepharose 4B-L-tyrosine-sulfonamide affinity column. CA I and II isozymes were treated with some anti-epileptic drugs, then the inhibition or activation of enzyme determined. The results of this study show that Levetiracetam is the most effective inhibitor for human erythrocytes carbonic anhydrase compared with the other anti-epileptic drugs.
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Anticonvulsivantes/farmacología , Anhidrasa Carbónica II/antagonistas & inhibidores , Anhidrasa Carbónica I/antagonistas & inhibidores , Inhibidores de Anhidrasa Carbónica/farmacología , Eritrocitos/efectos de los fármacos , Electroforesis en Gel de Poliacrilamida , Eritrocitos/enzimología , Humanos , IsoenzimasRESUMEN
Varenicline is a new drug for smoking cessation, and its effect on epilepsy is not clear. The aim of this study was to investigate whether different doses of varenicline cause epileptic activity. Forty rats were randomly assigned to the following eight groups: control, saline, and 0.025, 0.04, 0.1, 0.5, 1, and 2 mg kg(-1) varenicline (single dose, i.p.). EEGs were recorded before the varenicline injection and during the following 240 min. While epileptic discharges were observed on the EEGs of the rats in all of the varenicline-treated groups, motor findings of epileptic seizure were not observed in some rats in these groups except the 1 and 2 mg kg(-1) groups. These findings indicate that different single doses of varenicline cause epileptic activity in rats.
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Benzazepinas/toxicidad , Encéfalo/efectos de los fármacos , Epilepsia/inducido químicamente , Agonistas Nicotínicos/toxicidad , Quinoxalinas/toxicidad , Convulsiones/inducido químicamente , Animales , Benzazepinas/administración & dosificación , Relación Dosis-Respuesta a Droga , Electroencefalografía , Masculino , Agonistas Nicotínicos/administración & dosificación , Quinoxalinas/administración & dosificación , Ratas , Ratas Wistar , VareniclinaRESUMEN
Altitude training is frequently used by athletes to improve sea-level performance. However, the objective benefits of altitude training are controversial. This study aimed to investigate the possible alterations in hemorheological parameters in response to altitude training. Sprague Dawley rats, were divided into 6 groups: live low-train low (LLTL), live high-train high (LHTH), live high-train low (LHTL) and their controls live high and low (LHALC), live high (LHC), live low (LLC). LHC and LHTH groups were exposed to hypoxia (15% O2, altitudes of 3000 m), 4 weeks. LHALC and LHTL were exposed to 12 hours hypoxia/normoxia per day, 4 weeks. Hypoxia was maintained by a hypoxic tent. The training protocol corresponded to 60-70% of maximal exercise capacity. Rats of training groups ran on treadmill for 20-30 min/day, 4 days/week, 4 weeks. Erythrocyte deformability of LHC group was increased compared to LHALC and LLC. Deformability of LHTH group was higher than LHALC and LLTL groups. No statistically significant alteration in erythrocyte aggregation parameters was observed. There were no significant relationships between RBC deformability and exercise performance. The results of this study show that, living (LHC) and training at altitude (LHTH) seems more advantageous in hemorheological point of view.
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Altitud , Agregación Eritrocitaria/fisiología , Eritrocitos/fisiología , Hipoxia/sangre , Condicionamiento Físico Animal , Animales , Rendimiento Atlético , Modelos Animales de Enfermedad , Masculino , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley , Reología/métodosRESUMEN
This study aimed to investigate alterations in hemorheology induced by L-carnosine, an anti- oxidant dipeptide, and to determine their relationship to oxidative stress in density-separated erythrocytes of aged and young rats. 28 male Sprague Dawley rats were divided into 4 groups as aged (Aca), young (Yca) L-carnosine groups (250 mg/kg L-carnosine, i.p.) and aged (As), young (Ys) control groups (saline, i.p.). Density separation was further performed to these groups in order to separate erythrocytes according to their age. Blood samples were used for the determination of erythrocyte deformability, aggregation; and oxidative stress parameters. Erythrocyte deformability of Yca group measured at 0.53 Pa was lower than Aca group. Similarly, deformability of least-dense (young) erythrocytes of Yca group was decreased compared to least-dense erythrocytes of Aca groups. Total antioxidant capacity (TAC) of Aca group was higher and oxidative stress index (OSI) lower than As group. Although L-carnosine resulted in an enhancement in TAC of aged rats, this favorable effect was not observed in erythrocyte deformability and aggregation in the dose applied in this study.
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Carnosina/farmacología , Senescencia Celular/fisiología , Agregación Eritrocitaria/efectos de los fármacos , Deformación Eritrocítica/efectos de los fármacos , Factores de Edad , Animales , Masculino , Estrés Oxidativo/fisiología , Ratas , Ratas Sprague-DawleyRESUMEN
It is known that hypoxia has a negative effect on nervous system functions, but exercise and DHA (docosahexaenoic acid) have positive effect. In this study, it was investigated whether exercise and/or DHA can prevent the effects of hypoxia on EEG and nerve conduction velocity (NCV). 35 adult Wistar albino male rats were divided into five groups (n=7): control (C), hypoxia (H), hypoxia and exercise (HE), hypoxia and DHA (HD), and hypoxia and exercise and DHA (HED) groups. During the 28-day hypoxia exposure, the HE and HED groups of rats were exercised (0% incline, 30 m/min speed, 20 min/day, 5 days a week). In addition, DHA (36 mg/kg/day) was given by oral gavage to rats in the HD and HED groups. While EEG records were taken before and after the experimental period, NCV records were taken after the experimental period from anesthetized rats. Data were analyzed by paired t-test, one-way ANOVA, and post hoc Tukey test. In this study, it was shown that exposure to hypoxia decreased theta activity and NCV, but exercise and DHA reduced the delta activity, while theta, alpha, beta activities, and NCV were increased. These results have shown that the effects of hypoxia exposure on EEG and NCV can be prevented by exercise and/or DHA.
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Ácidos Docosahexaenoicos/farmacología , Electroencefalografía/efectos de los fármacos , Hipoxia/fisiopatología , Conducción Nerviosa/fisiología , Condicionamiento Físico Animal/fisiología , Animales , Hipoxia/sangre , Masculino , Conducción Nerviosa/efectos de los fármacos , Oxígeno/sangre , Ratas , Ratas WistarRESUMEN
Inconsistency in consecutive blood pressure values is one of the most frequently observed problems in tail cuff method. The aim of this study was to measure blood pressure using the tail cuff method in rats without heating, anesthesia, and movement restriction. In this study, it has been shown that blood pressure measurement could be obtained without problem using the tail cuff method in freely moving rats in their cage environment. Also, the reliability of consecutive blood pressure values obtained from freely moving rats was higher than ether anesthesia and restricted groups.
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Determinación de la Presión Sanguínea/veterinaria , Animales , Presión Sanguínea , Determinación de la Presión Sanguínea/instrumentación , Determinación de la Presión Sanguínea/métodos , Temperatura Corporal , Frecuencia Cardíaca , Calor , Masculino , Actividad Motora , Ratas , Ratas Sprague-Dawley , Reproducibilidad de los Resultados , Cola (estructura animal)/irrigación sanguínea , Factores de TiempoRESUMEN
OBJECTIVE: At present, the precise pathophysiology of the slow coronary flow phenomenon (SCFP) is still unknown and there is no consensus as to how it should be treated. The rheological factors affect the clinical course of various cardiovascular diseases. We studied the intrinsic properties of blood in the SCFP. MATERIALS AND METHODS: Twenty-six SCFP patients who had angiographically confirmed SCFP, and had otherwise normal epicardial coronary arteries, were included in our study, as were 30 healthy individuals with normal results from arteriography. Red blood cell (RBC) deformability, aggregation, whole-blood viscosity at both native and standard (40%) hematocrit, and plasma viscosity were determined in each individual. The results were analyzed using a Mann-Whitney U-test, an unpaired t-test, and a χ-test, where appropriate. RESULTS: The mean thrombolysis in myocardial infarction frame count was significantly higher in SCFP patients than in the controls. RBC deformability measured at five different shear rates was significantly higher in SCFP patients than in the controls. The RBC aggregation index was lower in SCFP patients. There were no statistically significant differences in RBC aggregation half-time (t1/2) and aggregation amplitude, whole-blood viscosity, and plasma viscosity between the two groups. CONCLUSION: The SCFP is associated with increased RBC deformability and decreased RBC aggregation. These hemorheological alterations, possibly also contributing factors in limiting the pathogenesis, can especially serve as beneficial adaptive mechanisms in the SCFP.
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Circulación Coronaria , Agregación Eritrocitaria , Deformación Eritrocítica , Fenómeno de no Reflujo/sangre , Adaptación Fisiológica , Viscosidad Sanguínea , Estudios de Casos y Controles , Distribución de Chi-Cuadrado , Cineangiografía , Angiografía Coronaria , Femenino , Hematócrito , Humanos , Masculino , Persona de Mediana Edad , Fenómeno de no Reflujo/diagnóstico por imagen , Fenómeno de no Reflujo/fisiopatologíaRESUMEN
Orexins have been implicated in the regulation of sleep-wake cycle, energy homeostasis, drinking behavior, analgesia, attention, learning and memory but their effects on epileptic activity are controversial. We investigated whether intracortical injections of orexin A (100 pmol) and B (100 pmol) cause epileptic activity in rats. We observed epileptic seizure findings on these two groups rats. Orexin A and B also significantly increased total EEG power spectrum. Our findings indicate that orexins cause epileptic activity.
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Epilepsia/inducido químicamente , Péptidos y Proteínas de Señalización Intracelular/efectos adversos , Neuropéptidos/efectos adversos , Neurotransmisores/efectos adversos , Animales , Modelos Animales de Enfermedad , Electroencefalografía , Epilepsia/fisiopatología , Inyecciones Intraventriculares , Péptidos y Proteínas de Señalización Intracelular/administración & dosificación , Masculino , Neuropéptidos/administración & dosificación , Neurotransmisores/administración & dosificación , Orexinas , Ratas , Ratas WistarRESUMEN
BACKGROUND: This study aimed to explore the effects of progressive resistance exercise training (PRET) on hemorheology. MATERIAL/METHODS: Exercise sessions included 1-3 sets of 8-12 repetitions at 40-60% of 1-repetition maximum (1-RM) for 3 weeks and at 75-80% of 1-RM during weeks 4-12. Red blood cell (RBC) deformability and aggregation were determined by ektacytometry, plasma and whole blood viscosities (WBV) by rotational viscometry. Lactate concentration was evaluated by an analyzer and fibrinogen was evaluated by coagulometry. Plasma total oxidant/antioxidant status was measured by colorimetry. RESULTS: Following an acute increase after exercise on the first day, RBC deformability was elevated during weeks 3 and 4 (p=0.028; p=0.034, respectively). The last exercise protocol applied in week 12 again caused an acute increase in this parameter (p=0.034). RBC aggregation was increased acutely on the first day, but decreased after that throughout the protocol (p<0.05). At weeks 4 and 12 pre-exercise measurements of WBV at standard hematocrit and plasma viscosity were decreased (p=0.05; p=0.041, respectively), while post-exercise values were increased (p=0.005; p=0.04, respectively). Post-exercise WBV at autologous hematocrit measured at week 12 was increased (p=0.01). Lactate was elevated after each exercise session (p<0.05). Fibrinogen was decreased on the third week (p<0.01), while it was increased on the 4th week (p=0.005). Plasma antioxidant status was increased at week 3 (p=0.034) and oxidative stress index was decreased at week 4 (p=0.013) after exercise. CONCLUSIONS: The results of this study indicate that PRET may have positive effects on hemorheological parameters.
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Ejercicio Físico/fisiología , Salud , Hemorreología/fisiología , Entrenamiento de Fuerza , Antioxidantes/metabolismo , Viscosidad Sanguínea/fisiología , Agregación Eritrocitaria/fisiología , Deformación Eritrocítica/fisiología , Eritrocitos/fisiología , Fibrinógeno/metabolismo , Humanos , Ácido Láctico/sangre , Masculino , Oxidantes/sangre , Estrés Oxidativo , Oxígeno/metabolismo , Factores de Tiempo , Adulto JovenRESUMEN
Orexins have been implicated with physiological function including sleep-wake cycle, energy homeostasis, drinking behavior, analgesia, attention, learning and memory but their effects on excitability are controversial. We investigated the effects of intracortical injections of orexin A (100 pmol) and B (100 pmol) on the electrophysiological manifestation of epileptic seizures induced by cortical penicillin application in adult male rats. In comparison to saline, orexin A and B enhanced significantly the spike number, spike amplitude and spectral power values induced by cortical penicillin. Our findings indicates that orexins enhances the hyperexcitable and hypersyncronic cortical epileptic activity induced by focal application of penicillin-G.
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Conducta Animal/efectos de los fármacos , Corteza Cerebral/efectos de los fármacos , Epilepsia/fisiopatología , Péptidos y Proteínas de Señalización Intracelular/efectos adversos , Neuropéptidos/efectos adversos , Animales , Corteza Cerebral/fisiopatología , Electroencefalografía , Epilepsia/inducido químicamente , Infusiones Intraventriculares , Péptidos y Proteínas de Señalización Intracelular/administración & dosificación , Masculino , Neuropéptidos/administración & dosificación , Neurotransmisores/administración & dosificación , Neurotransmisores/efectos adversos , Orexinas , Penicilina G/administración & dosificación , Penicilina G/efectos adversos , Ratas , Ratas WistarRESUMEN
OBJECTIVE: The aim of this study was to investigate the effects of the electromagnetic field generated from the 1800 MHz radiofrequency radiation (EF) on erythrocyte rheological parameters and erythrocyte zinc levels. MATERIAL AND METHODS: Twenty-four male Wistar Albino rats were randomly grouped as follows: 1) two control groups and 2) study groups: i) Group A: EF exposed group (2.5 h/day for 30 days, the phone on stand-by), and ii) Group B: EF exposed group (2.5 min/day for 30 days, the phone ringing in silent mode). At the end of the experimental period erythrocyte rheological parameters such as erythrocyte deformability and aggregation were determined by an ectacytometer. Erythrocyte zinc level, which affects hemorheological parameters, was also measured by atomic absorption spectrophotometer. RESULTS: Erythrocyte deformability was decreased in both study groups but the decrease in group A was not statistically significant. Exposure to EF did not have any significant effect on erythrocyte aggregation. On the other hand, erythrocyte zinc level was significantly reduced in both study groups. CONCLUSION: Exposure to EF may have decreased tissue oxygenation due to reduced erythrocyte deformability. Decrease in erythrocyte zinc level may have caused the impairment in erythrocyte deformability.
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BACKGROUND: This study aimed to investigate alterations in hemorheology by cold exposure, in vivo and ex vivo, and to determine their relationship to oxidative stress. MATERIAL/METHODS: Rats were divided into 2 in vivo and ex vivo cold exposure groups. The in vivo group was further divided into control (AR), AC (4°C, 2 hours) and ALTC (4°C, 6 hours) subgroups; and the ex vivo group was divided into control (BR) and BC (4°C, 2 hours) subgroups. Blood samples were used for the determination of erythrocyte deformability, aggregation, and oxidative stress parameters. RESULTS: Erythrocyte deformability and aggregation were not affected by 2-hour ex vivo cold exposure. While 2 hour in vivo cold exposure reduced erythrocyte deformability, it returned to normal after 6 hours, possibly due the compensation by acute neuroendocrine response. Six hours of cold exposure decreased aggregation index, and might be an adaptive mechanism allowing the continuation of circulation. Aggregation of ex vivo groups was lower compared to in vivo groups. Cold exposure at various temperatures did not cause alterations in plasma total oxidant antioxidant status and oxidative stress index (TOS, TAS, OSI) when considered together. CONCLUSIONS: Results of this study indicate that the alterations observed in hemorheological parameters due to cold exposure are far from being explained by the oxidative stress parameters determined herein.
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Frío , Agregación Eritrocitaria , Deformación Eritrocítica , Hemorreología , Animales , Antioxidantes/metabolismo , Oxidantes/sangre , Estrés Oxidativo , Distribución Aleatoria , Ratas , Ratas Sprague-DawleyRESUMEN
BACKGROUND: In this study we examined the effects of docosahexaenoic acid (DHA) on growth hormone (GH), insulin-like growth factor I (IGF-I) and insulin-like growth factor binding protein-3 (IGFBP-3) in response to chronic hypoxia and exercise training in hypoxic conditions. METHODS: Thirty-five rats were divided into five groups; control group (C), hypoxia group (H), hypoxia-exercise group (HE), hypoxia-docosahexaenoic acid group (HD), hypoxia-exercise-docosahexaenoic acid group (HED). A treadmill exercise was performed as 30 m/min for 20 min/day, 5 days per week for 28 days at level grade for the exercising groups (HE and HED). DHA was given to the HD and HED groups every day orally (36 mg/kg). The animals, except for the C group, were exposed to hypoxia for 28 days. RESULTS: Serum levels of GH and IGF-I in the H group decreased after chronic hypoxia (p<0.001). GH and IGF-I in the HD group also decreased compared with the C group (p<0.05, p<0.01, respectively). GH in C group did not show significant difference compared with the HE and HED groups. Decreased serum level of IGF-I was observed for the HED group (p<0.05). CONCLUSIONS: According to our findings, chronic hypoxia exposure decreases serum levels of GH, and IGF-I and exercise training have a slightly positive effect on GH/IGF-I axis during hypoxia. In addition, DHA supplementation slightly increases GH and IGF-I serum levels in hypoxic conditions. However, this effect on GH/IGF-I axis during hypoxia is not strong compared with exercise. Therefore, we concluded that exercise and/or DHA supplementation does not have additional positive effect on these hormones in hypoxic conditions.
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Suplementos Dietéticos , Ácidos Docosahexaenoicos/farmacología , Hormona del Crecimiento/sangre , Hipoxia/tratamiento farmacológico , Factor I del Crecimiento Similar a la Insulina/metabolismo , Esfuerzo Físico , Animales , Enfermedad Crónica , Modelos Animales de Enfermedad , Hipoxia/sangre , Hipoxia/fisiopatología , Proteína 3 de Unión a Factor de Crecimiento Similar a la Insulina/sangre , Masculino , Ratas , Ratas Wistar , Factores de TiempoRESUMEN
This study aimed to investigate the short term effects of carbon monoxide (CO) poisoning and three kinds of poisoning treatments; namely room air, normobaric and hyperbaric oxygen on hemorheological parameters such as red blood cell (RBC) deformability, aggregation, blood and plasma viscosity. 43 Wistar rats were divided into 5 groups. Poisoning was induced by exposure to 4000 ppm CO (1 h). The poisoning protocol was followed by 3 types of treatments; room air, normobaric 100% oxygen and hyperbaric oxygen for 1 h. RBC deformability and aggregation were determined using an ektacytometer (LORCA) and a cone-plate rotational viscometer was used for the viscosity measurements. RBC deformability of CO poisoned rats were found to be elevated and the treatments applied, caused decrement of this parameter. A no significant increment tendency was found in erythrocyte aggregation after CO exposure. Although room air and hyperbaric oxygen treatments caused further significant elevations in the amplitude of aggregation, normobaric oxygen therapy induced decrement in this parameter towards control levels. No significant alterations were observed in viscosity values among the groups. The results of this study demonstrate normobaric oxygen therapy as a better choice of treatment after CO poisoning in hemorheological point of view.
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Intoxicación por Monóxido de Carbono/sangre , Intoxicación por Monóxido de Carbono/terapia , Terapia por Inhalación de Oxígeno/métodos , Animales , Viscosidad Sanguínea , Monóxido de Carbono/administración & dosificación , Carboxihemoglobina/metabolismo , Agregación Eritrocitaria , Deformación Eritrocítica , Hemorreología , Oxigenoterapia Hiperbárica/métodos , Distribución Aleatoria , Ratas , Ratas WistarRESUMEN
It is known that aging is associated with marked effects on integrity and function of cell membrane. These effects may also be exacerbated by exogenous chemicals, e.g. sulfite. Thus, the aim of this paper is to examine the influence of sulfite on hemorheological and related hematological parameters in rats of various ages. In this study, male Wistar rats at the age of 3 and 18 months were used and the following parameters were evaluated: Mean Cell Volume (MCV), Mean Corpuscular Hemoglobin Concentration (MCHC), Red blood Cell (RBC) deformability and aggregation. The results show that aging is associated with a decrease in RBC deformability and MCHC, an increase in MCV. Sulfite administration significantly increased RBC deformability in both young and aged rats. Although MCHC was decreased in young rats, it was increased in aged rats in response to sulfite exposure. Additionally, sulfite induced a decrement in MCV of aged rats. Neither aging nor sulfite treatment caused significant alterations in RBC aggregation parameters in all experimental groups. In conclusion, these findings suggest that RBC deformability impairs with age and sulfite has ameliorating effects on RBC deformability in both young and aged rats.