RESUMEN
BACKGROUND: Malassezia furfur is lipodependent yeast like fungus that causes superficial mycoses such as pityriasis versicolor and dandruff. Nevertheless, there are no standard reference methods to perform susceptibility test of Malassezia species yet. AIMS: Therefore, in this study, we evaluated the optimized culture medium for growth of this lipophilic yeast using modified leeming-Notman agar and colorimetric resazurin microtiter assay to assess antimycotic activity of fluconazole against M. furfur. RESULTS: The result showed that these assays were more adjustable for M. furfur with reliable and reproducible MIC end-point, by confirming antimycotic activity of fluconazole with MIC of 2µg/ml. CONCLUSION: We conclude that this method is considered as the rapid and effective susceptibility testing of M. furfur with fluconazole antifungal activity.
Asunto(s)
Antifúngicos/farmacología , Fluconazol/farmacología , Malassezia/efectos de los fármacos , Oxazinas/química , Xantenos/química , Colorimetría/métodos , Medios de Cultivo/química , Dermatomicosis/microbiología , Humanos , Malassezia/crecimiento & desarrollo , Malassezia/fisiología , Pruebas de Sensibilidad Microbiana/métodos , Tiña Versicolor/microbiologíaRESUMEN
The importance of Hospital Infection Control Committees (HICC) increases every year due to the emergence of multiresistant bacterial strains, hospital outbreaks, and other factors that cause HI. This demonstrates the fragility of the quality of hospital and medical care. Having a well-organized HICC benefits hospitals by improving quality, lowering costs and, most important, reducing patient morbidity and mortality. This review records the history of the development of HICCs, their present organizational structure, and offers recommendations for hte best methods of infection surveillance.
