Grafting of aniline derivatives onto chitosan and their applications for removal of reactive dyes from industrial effluents.

A series of chitosan-grafted polyaniline derivatives {chitosan-g-polyaniline (CS-g-PANI), chitosan-g-poly(N-methylaniline) (CS-g-PNMANI), and chitosan-g-poly(N-ethylaniline) (CS-g-PNEANI)} were synthesized by in situ chemical oxidation polymerization method. The synthesized copolymers were analyzed by means of Fourier transform infrared (FTIR), and ultraviolet-visible (UV-vis) spectroscopies, thermogravimetric analysis (TGA), and field emission scanning electron microscopy (FE-SEM). These copolymers were applied as adsorbent for removal of acid red 4 (AR4) and direct red 23 (DR23) from aqueous solutions. The adsorption processes were optimized in terms of pH, adsorbent amount, and dyes concentrations. The maximum adsorption capacities (Qm) for the synthesized copolymers were calculated, and among them the CS-g-PNEANI sample showed highest Qm for both AR4 (98mgg-1) and DR23 (112mgg-1) dyes. The adsorption kinetics of AR4 and DR23 dyes follow the pseudo-second order kinetic model. The regeneration and reusability tests revealed that the synthesized adsorbents had the relatively good reusability after five repetitions of the adsorption-desorption cycles. As the results, it is expected that the CS-g-PANIs find application for removal of reactive dyes (especially anionic dyes) from industrial effluents mainly due to their low production costs and high adsorption effectiveness.

Separation and quantitative determination of cinacalcet metabolites in urine sample using RP-HPLC after derivation with a fluorescent labeling reagent.

In this investigation, a novel strategy for separation and quantitative determination of four metabolites of cinacalcet (M2a-Glu, M2b-Glu, M7-Gly, and M8-Gly) in human urine is suggested. The analytical assay is based on a pre-column derivation procedure of cinacalcet metabolites with 1-pyrenyldiazomethane (PDAM) as a fluorescent labeling reagent, and subsequently separation and quantitative determination with reverse-phase high-performance liquid chromatography (RP-HPLC) coupled with a fluorescence detector. Metabolites were separated on a Microsorb-MV 100-5 C18 chromatography column (250×4.6mm, 5µm) using acetate buffer (pH 3.5):methanol (30:70 v/v) as mobile phase at a flow rate of 1.0mLmin(-1). The method was fully validated in terms of linearity (r(2)>0.996; 1-10ngmL(-1)), precision (both intra-day and inter-day; RSD<6.2%), accuracy (92-110%), specificity, robustness (0.15%