RESUMEN
A method for detection of cancer-associated protein D-NFATc1 in serum using nanowire (NW) biosensor based on field-effect nanotransistor is developed. Field-effect nanotransistor was fabricated on the basis of «silicon-on-insulator¼ structures. For the biospecific detection of target protein, the NW surface was modified with aptamers against the target protein. Using the 3 um-NW enabled to obtain stable source-drain characteristics and to register D-NFATc1 in serum at concentration of 2.5 x 1014 M in the mode of drain-source current vs. gate voltage characteristics measurements. Data collection in the mode of drain-source current vs. gate voltage characteristics measurements was carried out with the use of high-speed data collection system running TURBO NBS software.
Asunto(s)
Técnicas Biosensibles , Factores de Transcripción NFATC/sangre , Nanocables , Proteínas de Neoplasias/sangre , Programas Informáticos , Transistores Electrónicos , Técnicas Biosensibles/instrumentación , Técnicas Biosensibles/métodos , Humanos , Sensibilidad y EspecificidadRESUMEN
The nanowire (NW) detection is one of fast-acting and high-sensitive methods allowing to reveal potentially relevant protein molecules. A NW biosensor based on the silicon-on-insulator (SOI)-structures was used for biospecific label-free detection of NFAT 1 (D-NFAT 1) oncomarker in real time. For this purpose, SOI-nanowires (NWs) were modified with aptamers against NFAT 1 used as molecular probes. It was shown that using this biosensor it is possible to reach the sensitivity of ~10(-15) M. This sensitivity was comparable with that of the NW biosensor with immobilized antibodies used as macromolecular probes. The results demonstrate promising approaches used to form the sensor elements for high-sensitive disease diagnostics.
Asunto(s)
Aptámeros de Nucleótidos/química , Técnicas Biosensibles/instrumentación , Factores de Transcripción NFATC/análisis , Nanocables/química , Proteínas de Neoplasias/análisis , Anticuerpos Monoclonales/química , Aptámeros de Nucleótidos/síntesis química , Técnicas Biosensibles/economía , Técnicas Electroquímicas , Humanos , Límite de Detección , Dióxido de Silicio/química , Soluciones , Succinimidas/químicaRESUMEN
Investigation of the phase-growth kinetics and identification of the phases formed as a result of reactive diffusion in the system of Si single crystals and Mo thin films, has been carried out by electrochemical anodic etching.
