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1.
Org Biomol Chem ; 21(37): 7593-7596, 2023 Sep 27.
Artículo en Inglés | MEDLINE | ID: mdl-37674470

RESUMEN

By opening the ring of a benzothiazole salt, we provide a sulfur source for the bifunctional reaction of styrene. The ring-opening-recombination reaction of the benzothiazole salt simultaneously constructs new C-S, C-O, and CO bonds after C-S bond breaking. The reaction proceeds in green solvents, requires no transition metal catalyst, and is compatible with many functional groups.

2.
Arch Virol ; 165(6): 1489-1494, 2020 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-32266550

RESUMEN

We detected a novel bovine hepacivirus N (HNV) subtype, IME_BovHep_01, in the serum of cattle in Tengchong, Yunnan, China, by high-throughput sequencing. The complete genome of IME_BovHep_01, was sequenced using an Illumina MiSeq sequencer and found to be 8850 nt in length, encoding one hypothetical protein. BLASTn analysis showed that the genome sequence shared similarity with the bovine hepacivirus isolate BovHepV_209/Ger/2014, with 88% query coverage and 70.8% identity. However, the highest similarity was to bovine hepacivirus N strain BRBovHep_RS963, for which only a partial genome sequence is available, with 68% query coverage and 81.5% identity. Sequence comparisons and phylogenetic analysis suggested that IME_BovHep_01 is a novel HNV subtype. Importantly, IME_BovHep_01 is the first member of this new genotype for which the complete genome sequence was determined.


Asunto(s)
Bovinos/virología , Genoma Viral , Hepacivirus/genética , Hepacivirus/aislamiento & purificación , Animales , Secuencia de Bases , China , Variación Genética , Genotipo , Hepacivirus/clasificación , Filogenia , Análisis de Secuencia de ADN , Secuenciación Completa del Genoma
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