Impact of Charlson Comorbidity Index on in-hospital mortality of patients with hyperglycemic crises: A propensity score matching analysis.

AIM: This study was designed to investigate the association between Charlson Comorbidity Index (CCI) and in-hospital mortality and other clinical outcomes among patients with hyperglycemic crises. METHOD: This retrospective cohort study was conducted using data from electric medical records. A total of 1668 diabetic patients with hyperglycemic crises from six tertiary hospitals met the inclusion criteria. CCI < 4 was defined as low CCI and CCI ≥ 4 was defined as high CCI. Propensity score matching (PSM) with the 1:1 nearest neighbour matching method and the caliper value of 0.02 was used to match the baseline characteristics of patients with high CCI and low CCI to reduce the confounding bias. In-hospital mortality, ICU admission, hypoglycemia, hypokalemia, acute kidney injury, length of stay (LOS), and hospitalisation expense between low CCI and high CCI were compared and assessed. Univariate and multivariate regression were applied to estimate the impact of CCI on in-hospital and other clinical outcomes. OUTCOME: One hundred twenty-one hyperglycemic crisis (HC) patients died with a mortality rate of 7.3%. After PSM, compared with low CCI, patients with high CCI suffered higher in-hospital mortality, ICU admission, LOS, and hospitalisation expenses. After multivariate regression, age (aOR: 1.12, 95% confidence interval [CI]: 1.06-1.18, p < 0.001), CCI(aOR: 4.42, 95% CI: 1.56-12.53, p = 0.005), uninsured (aOR: 22.32, 95% CI: 4.26-116.94, p < 0.001), shock (aOR: 10.57, 95% CI: 1.41-79.09, p = 0.022), mechanical ventilation (aOR: 75.29, 95% CI: 12.37-458.28, p < 0.001), and hypertension (aOR: 4.34, 95% CI: 1.37-13.82, p = 0.013) were independent risk factors of in-hospital mortality of HC patients. Besides, high CCI was an independent risk factor for higher ICU Admission (aOR: 5.91, 95% CI: 2.31-15.08, p < 0.001), hypoglycemia (aOR: 2.19, 95% CI:1.01-4.08, p = 0.049), longer LOS (aOR: 1.23, 95% CI: 1.19-2.27, p = 0.021), and higher hospitalisation expense (aOR: 2089.97, 95% CI: 193.33-3988.61, p = 0.031) of HC patients. CONCLUSION: CCI is associated with in-hospital mortality, ICU admission, hypoglycemia, LOS, and hospitalisation expense of HC patients. CCI could be an ideal indicator to identify, monitor, and manage chronic comorbidities among HC patients.

Relative saliency affects attentional capture and suppression of color and face singleton distractors: evidence from event-related potential studies.

Prior research has yet to fully elucidate the impact of varying relative saliency between target and distractor on attentional capture and suppression, along with their underlying neural mechanisms, especially when social (e.g. face) and perceptual (e.g. color) information interchangeably serve as singleton targets or distractors, competing for attention in a search array. Here, we employed an additional singleton paradigm to investigate the effects of relative saliency on attentional capture (as assessed by N2pc) and suppression (as assessed by PD) of color or face singleton distractors in a visual search task by recording event-related potentials. We found that face singleton distractors with higher relative saliency induced stronger attentional processing. Furthermore, enhancing the physical salience of colors using a bold color ring could enhance attentional processing toward color singleton distractors. Reducing the physical salience of facial stimuli by blurring weakened attentional processing toward face singleton distractors; however, blurring enhanced attentional processing toward color singleton distractors because of the change in relative saliency. In conclusion, the attentional processes of singleton distractors are affected by their relative saliency to singleton targets, with higher relative saliency of singleton distractors resulting in stronger attentional capture and suppression; faces, however, exhibit some specificity in attentional capture and suppression due to high social saliency.

Suppression on the basis of template for rejection is reactive: Evidence from human electrophysiology.

According to most theories of attention, the selection of task-relevant visual information can be enhanced by holding them in visual working memory (VWM). However, there has been a long-standing debate concerning whether similar optimization can also be achieved for task-irrelevant information, known as a "template for rejection". The present study aimed to explore this issue by examining the consequence of cue distractors before visual search tasks. For this endeavor, we manipulated the display heterogeneity by using two distractor conditions, salient and non-salient, to explore the extent to which holding the distractor color in VWM might affect attentional selection. We measured the reaction times of participants while their EEG activity was recorded. The results showed that WM-matched distractors did not improve reaction times but rather slowed them down in both tasks. Event-related potential (ERP) results showed that the display heterogeneity had no modulatory effect on the degree of distractor suppression. Even in the salient distractor condition, the WM-matched distractor received no greater suppression. Furthermore, the WM-matched distractor but not the neutral distractor elicited an N2pc before the PD in salient distractor conditions. This suggests that the template for rejection operates reactively since suppression occurs after extra attentional processes to the distractor. Moreover, the presence of WM-matched distractors led to a reduction of P3b, indicating a competition between target processing and WM-matched distractor rejection. Our findings provide insights into the mechanisms underlying the optimization of attentional selection, and have implications for future studies aimed at understanding the role of VWM in cognition.

Different prioritization states of working memory representations affect visual searches: Evidence from an event-related potential study.

Previous evidence has shown that the contents of working memory (WM) can bias visual selection. However, not much is known about how WM effects change when the WM representation is held in different prioritization states. Here, we investigated this problem using event-related potentials. Subjects maintained two colors in WM while performing a search task. One of the colors was retro-cued, indicating that it was 80 % likely to be the target of the memory test. During the search display, one of the distractors was a salient color singleton, and this singleton distractor could carry the same color as the cued WM representation, the uncued WM representation, or be irrelevant to the memory content. Behaviorally, the memory test performance was found to be better for the cued color than for the uncued color, and we observed lower search accuracy (ACC) and longer search reaction time (RT) when the singleton distractor matched the cued WM representation than when it matched an uncued or an irrelevant WM representation. Event-related potential (ERP) data showed that the P3 amplitude of cue-color distractor conditions was smaller than that of uncued-color distractor conditions and irrelevant-color distractor conditions. These findings clearly indicate that prioritizing an item for enhanced representational quality enables the item to bias attention to a greater extent.

A Sec-dependent effector, CLIBASIA_04425, contributes to virulence in 'Candidatus Liberibater asiaticus'.

Citrus Huanglongbing (HLB) is the most destructive citrus disease worldwide, mainly caused by 'Candidatus Liberibacter asiaticus' (CLas). It encodes a large number of Sec-dependent effectors that contribute to HLB progression. In this study, an elicitor triggering ROS burst and cell death in Nicotiana benthamiana, CLIBASIA_04425 (CLas4425), was identified. Of particular interest, its cell death-inducing activity is associated with its subcellular localization and the cytoplasmic receptor Botrytis-induced kinase 1 (BIK1). Compared with CLas infected psyllids, CLas4425 showed higher expression level in planta. The transient expression of CLas4425 in N. benthamiana and its overexpression in Citrus sinensis enhanced plant susceptibility to Pseudomonas syringae pv. tomato DC3000 ΔhopQ1-1 and CLas, respectively. Furthermore, the salicylic acid (SA) level along with the expression of genes NPR1/EDS1/NDR1/PRs in SA signal transduction was repressed in CLas4425 transgenic citrus plants. Taken together, CLas4425 is a virulence factor that promotes CLas proliferation, likely by interfering with SA-mediated plant immunity. The results obtained facilitate our understanding of CLas pathogenesis.

The impact of insurance status on in-hospital mortality in patients with hyperglycaemic crisis: A propensity score matching analysis.

AIM: This study was designed to determine the associations between insurance status and clinical outcomes among patients with hyperglycaemic crisis. METHODS: Overall, 1668 patients with hyperglycaemic crisis were recruited from the Chongqing Medical University Medical Data Science Academy's big data platform. In-hospital mortality, length of stay and complications (i.e., hypoglycaemia, hypokalemia, pulmonary infection, multiple systemic organ failure, acute kidney injury and deep venous thrombosis) were assessed. Propensity score matching analysis was used to reduce the confounding bias, and univariate and multivariate logistic regression were used to estimate the effect of insurance status on mortality in patients with hyperglycaemic crisis. RESULTS: After matching one uninsured patient to two insured patients with a calliper of 0.02, the uninsured group suffered a higher burden of in-hospital mortality than the insured group (16.9% vs. 9.8%); the insured status (odds ratio = 0.216, 95% confidence interval = 0.079-0.587) was a potential protect factor for in-hospital mortality of patients with hyperglycaemic crisis in the multivariate logistic regression analysis. CONCLUSIONS: Insurance status is associated with the outcomes of hospitalisation for hyperglycaemic crisis; uninsured patients with hyperglycaemic crisis face a higher risk of mortality in China.

Faces do not guide attention in an object-based facilitation manner.

Numerous studies on face processing have revealed their special ability to affect attention, but relatively little research has been done on how faces guide spatial attention allocation. To enrich this field, this study resorted to the object-based attention (OBA) effect in a modified double-rectangle paradigm where the rectangles were replaced with human faces and mosaic patterns (non-face objects). Experiment 1 replicated the typical OBA effect in the non-face objects, but this effect was absent in Asian and Caucasian faces. Experiment 2 removed the eye region from Asian faces, but still found no object-based facilitation in the faces without eyes. In Experiment 3, the OBA effect was also observed for faces when the faces disappear a short period before the responses. Overall, these results revealed that when two faces are presented together, they do not exert object-based facilitation regardless of their facial features such as race and the presence of eyes. We argue that the lack of a typical OBA effect is due to the filtering cost induced by the entire face content. This cost slows down the response when attention shifts within a face and results in the absence of object-based facilitation.

The application of artificial intelligence technology in education influences Chinese adolescent's emotional perception.

Humans need to accurately infer the intentions and feelings of others to engage in successful social interaction. However, the application of artificial intelligence technology in Education (AIEd) forms a human-machine collaborative environment which changed the interaction relationship of individuals, it may have an affect on them. This study aimed to explore whether AIEd affects adolescents' emotional perception. Combined with the actual teaching situation and the result of the questionnaire, 1332 students recruited through random sampling from AI Curriculum Reform Demonstration Schools in Guangzhou participated in this study. Different emotional priming stimulative materials (sentences and situational pictures) were used in the experiments. The task was designed to investigate adolescents' reaction time to emotional faces (positive, negative). After eliminating blank data and invalid data with response time greater than 150 ms, 977 and 962 valid data were included in the statistical analysis in experiment 1 and experiment 2 respectively. Results show that AIEd has a negative effect on adolescents' emotional perception. Prior research has focused on theory to the exclusion of practical applications and the psychological impact of AIEd, thus this study makes an innovative contribution in exploring the impact of the application of artificial intelligence technology in education on adolescents' physical and mental development by using empirical research methods.

An effector of 'Candidatus Liberibacter asiaticus' manipulates autophagy to promote bacterial infection.

Autophagy functions in plant host immunity responses to pathogen infection. The molecular mechanisms and functions used by the citrus Huanglongbing (HLB)-associated intracellular bacterium 'Candidatus Liberibacter asiaticus' (CLas) to manipulate autophagy are unknown. We identified a CLas effector, SDE4405 (CLIBASIA_04405), which contributes to HLB progression. 'Wanjincheng' orange (Citrus sinensis) transgenic plants expressing SDE4405 promotes CLas proliferation and symptom expression via suppressing host immunity responses. SDE4405 interacts with the ATG8-family of proteins (ATG8s), and their interactions activate autophagy in Nicotiana benthamiana. The occurrence of autophagy is also significantly enhanced in SDE4405-transgenic citrus plants. Interrupting NbATG8s-SDE4405 interaction by silencing of NbATG8c reduces Pseudomonas syringae pv. tomato strain DC3000ΔhopQ1-1 (Pst DC3000ΔhopQ1-1) proliferation in N. benthamiana, and transient overexpression of CsATG8c and SDE4405 in citrus promotes Xanthomonas citri subsp. citri (Xcc) multiplication, suggesting that SDE4405-ATG8s interaction negatively regulates plant defense. These results demonstrate the role of the CLas effector protein in manipulating autophagy, and provide new molecular insights into the interaction between CLas and citrus hosts.

Interaction between the flagellum of Candidatus Liberibacter asiaticus and the vitellogenin-like protein of Diaphorina citri significantly influences CLas titer.

Huanglongbing (HLB) is a global devastating citrus disease that is mainly caused by "Candidatus Liberibacter asiaticus" (CLas). It is mostly transmitted by the insect Asian citrus psyllid (ACP, Diaphorina citri) in a persistent and proliferative manner. CLas traverses multiple barriers to complete an infection cycle and is likely involved in multiple interactions with D. citri. However, the protein-protein interactions between CLas and D. citri are largely unknown. Here, we report on a vitellogenin-like protein (Vg_VWD) in D. citri that interacts with a CLas flagellum (flaA) protein. We found that Vg_VWD was upregulated in CLas-infected D. citri. Silencing of Vg_VWD in D. citri via RNAi silencing significantly increased the CLas titer, suggesting that Vg_VWD plays an important role in the CLas-D. citri interaction. Agrobacterium-mediated transient expression assays indicated that Vg_VWD inhibits BAX- and INF1-triggered necrosis and suppresses the callose deposition induced by flaA in Nicotiana benthamiana. These findings provide new insights into the molecular interaction between CLas and D. citri.

Saliency affects attentional capture and suppression of abrupt-onset and color singleton distractors: Evidence from event-related potential studies.

Attention is the process of selecting relevant information and suppressing irrelevant information. However, it is still controversial whether attentional capture by salient but task-irrelevant stimuli operates in a bottom-up fashion (stimulus-driven theory) or a top-down fashion (goal-driven theory) or if even salient distractors can be suppressed before capturing attention (signal suppression theory). In the present study, we investigated how saliency affects attentional capture (indexed by N2-posterior-contralateral [N2pc]) and suppression (indexed by distractor positivity [PD ]) of abrupt-onset and color singleton distractors in a visual search task. Experiment 1 showed that an abrupt-onset distractor elicited both N2pc and PD , while a color singleton distractor elicited only PD . Moreover, the abrupt-onset distractor elicited a larger N2pc and a larger PD relative to the color singleton distractor. In addition, both distractors elicited an early positive component, the positivity posterior contralateral (Ppc), which was also larger for abrupt onsets than for color singletons. Experiment 2 further demonstrated that when both the abrupt onset and color singleton were designed as targets, and thus required no attentional suppression, Ppc was elicited, but PD was not. This corroborated the finding in Experiment 1 that the later PD , not the early Ppc, reflected attentional suppression. Therefore, a more salient distractor demonstrates stronger early perceptual processing, can capture attention better and needs more attentional resources to be suppressed later. Based on these results, a three-stage hypothesis is proposed, in which the saliency of a distractor modulates processing at early perception, attentional capture, and suppression stages.

Influence of artificial intelligence in education on adolescents' social adaptability: The mediatory role of social support.

Artificial intelligence (AI) is widely used in the field of education at present, but people know little about its possible impacts, especially on the physical and mental development of the educated. It is important to explore the possible impacts of the application of artificial intelligence in education (AIEd) in order to avoid the possible adverse effects. Prior research has focused on theory to the exclusion of the psychological impact of AIEd, and the empirical research was relatively lacking. This study aimed to identify the influence of AIEd on adolescents' social adaptability via social support. A total of 1332 students were recruited using random sampling from 13 Artificial Intelligence Curriculum Reform Experimental Schools in Guangzhou, Southern China, completed the survey. There were 342 primary school students (Meanage = 10.6), 351 junior high school students (Meanage = 13.1), and 639 senior high school students (Meanage = 15.8). Results showed that AIEd has a negative impact on adolescents' social adaptability, and is significantly negatively correlated with social adaptability and family support, but there is no significant correlation with school support. AIEd could not only affect social adaptability directly, but also could affected it through the family support.

Neural correlates of conscious processing of emotional faces: Evidence from event-related potentials.

There is a theoretical debate between the early and late neural correlates of consciousness (NCCs). Previous studies using neutral face stimuli supported an early NCC and suggested that visual awareness negativity (VAN) is associated with consciousness, while late positivity (LP) reflects post-perceptual activity. However, emotional faces may help to examine the relationship between LP and consciousness due to the differences in late processing between emotional and neutral faces. To explore the effects of facial emotional information on NCCs, the present study manipulated consciousness with the inattentional blindness paradigm and used happy, fearful, and neutral faces as visual stimuli. The results showed that the conscious processing of emotional faces was correlated with VAN and LP, while the conscious processing of neutral faces was associated with VAN. First, the results suggest that VAN is an NCC, and the relationship between LP and consciousness is affected by facial emotional information. Second, VAN reflects the early perceptual experience of emotional faces, whereas LP may reflect the late conscious processing of emotional faces. Furthermore, source localization analysis showed that the LPs of emotional faces were mainly located in the frontal and parietal lobes, whereas those of neutral faces showed no significant activation. This suggests that facial emotional information may affect the brain regions associated with conscious processing. Time-frequency analysis showed that conscious processing is related to the enhancement of alpha and theta oscillation, indicating that conscious processing may be associated with the suppression of irrelevant stimuli. Overall, the present study suggests that the integration of the theories that support early and late NCCs helps explain the conscious processing of emotional faces.

Contextual uncertainty of visual scene modulates object-based attention.

Contextual information involves invariant properties that are critical in selective attention. There is no direct evidence showing the effect of contextual information on object-based selective attention. The current study aimed to investigate the role of contextual uncertainty on object-based effect using a flanker task and to clarify the contradictory results obtained in previous studies. Herein, contextual uncertainty specifically referred to the configurations of the stimuli presented randomly as vertical or horizontal displays (high contextual uncertainty) that was reduced by showing consistent configurations within a block, via implicit learning of configuration (low contextual uncertainty). In Experiment 1, the object-based effect was observed under the high uncertainty condition and disappeared under the low uncertainty condition, demonstrating that contextual uncertainty modulated object-based attention. Experiment 2 provided explicit knowledge of the configural orientations, which can be utilised to sufficiently guide subsequent perception with increase in cueing interval, and therefore, affected contextual uncertainty. Relative to a short cueing interval, the long cueing interval enabled the participants to utilise the contextual knowledge for guiding visual attention and reducing uncertainty. Consistent with the finding in Experiment 1, the explicit manipulation of contextual uncertainty affected the object-based effect. The results proved that the contextual uncertainty played an important role in prioritisation in the object-based attentional selection. The mechanism of the interplay between contextual uncertainty and object-based attention was discussed.

The Role of Attentional Bias Toward Negative Emotional Information and Social Anxiety in Problematic Social Media Use.

The current study aimed to analyze whether individuals with problematic social media use (PSMU) demonstrate attentional bias (AB) toward negative emotional information and determine the relationships among the severity of PSMU, social anxiety, and negative AB. Sixty participants were divided into problematic and normal use groups according to their scores on the Bergen Social Media Addiction Scale (BSMAS). The BSMAS and Interaction Anxiety Scale were adopted to measure the severity of PSMU and social anxiety, respectively. An emotional Stroop task and a visual dot-probe task (DPT) were used to assess AB toward negative emotional expressions. Relationships among the severity of PSMU, social anxiety, and negative AB were investigated using Pearson's correlation coefficient. Results showed that individuals with PSMU demonstrated AB toward negative emotional information in the emotional DPT but not in the emotional Stroop task. AB toward negative emotional information was positively correlated with the severity of PSMU and social anxiety in the emotional DPT. Findings support the key role of negative AB and social anxiety in individuals with PSMU, suggesting that more attention be paid to negative AB and social anxiety for the prevention and treatment of PSMU. [Journal of Psychosocial Nursing and Mental Health Services, 61(6), 33-42.].

Development and validation of a predictive model for diagnosing prostate cancer after transperineal prostate biopsy.

Objective: This study aimed to develop and validate a nomogram to predict the probability of prostate cancer (PCa) after transperineal prostate biopsy by combining patient clinical information and biomarkers. Methods: First, we retrospectively collected the clinicopathologic data from 475 patients who underwent prostate biopsy at our hospital between January 2019 to August 2021. Univariate and multivariate logistic regression analyses were used to select risk factors. Then, we established the nomogram prediction model based on the risk factors. The model performance was assessed by receiver operating characteristic (ROC) curves, calibration plots and the Hosmer-Lemeshow test. Decision curve analysis (DCA) was used to evaluate the net benefit of the model at different threshold probabilities. The model was validated in an independent cohort of 197 patients between September 2021 and June 2022. Results: The univariate and multivariate logistic regression analyses based on the development cohort indicated that the model should include the following factors: age (OR = 1.056, p = 0.001), NEUT (OR = 0.787, p = 0.008), HPR (OR = 0.139, p < 0.001), free/total (f/T) PSA (OR = 0.013, p = 0.015), and PI-RADS (OR = 3.356, p < 0.001). The calibration curve revealed great agreement. The internal nomogram validation showed that the C-index was 0.851 (95% CI 0.809-0.894). Additionally, the AUC was 0.851 (95% CI 0.809-0.894), and the Hosmer-Lemeshow test result presented p = 0.143 > 0.05. Finally, according to decision curve analysis, the model was clinically beneficial. Conclusion: Herein, we provided a nomogram combining patients' clinical data with biomarkers to help diagnose prostate cancers.

Attentional Bias Is Associated with Negative Emotions in Problematic Users of Social Media as Measured by a Dot-Probe Task.

OBJECTIVE: Social media (SM) have flourished and are affecting human lives on an unprecedented scale. Problematic social media use (PSMU) is a recently emerging problematic behavior that affects both physical and mental health. The purpose of this study was to investigate whether problematic users of SM display attentional bias (AB) toward SM-related cues, as well as the relationships between AB, the severity of PSMU, and negative emotions. METHOD: 1000 college students were recruited through classes and online forums and then tested using the Bergen Social Media Addiction Scale (BSMAS). Eventually, 60 participants, identified by the cut-off point for BSMAS, consented to participate in the study and were divided into two groups (i.e., a problematic use group and a typical use group). The severity of PSMU and negative emotions (i.e., anxiety, depression, social fear, and loneliness) were evaluated by self-report questionnaires. AB was assessed by an addiction Stroop task and a dot-probe task (DPT). RESULTS: PSMU was found to be positively associated with anxiety (r = 0.28, p < 0.05), depression (r = 0.35, p < 0.01), and social fear (r = 0.38, p < 0.01), but not with loneliness (r = 0.19, p = 0.15). Participants with a tendency toward PSMU displayed AB toward SM-related cues in the DPT [F (1, 58) = 26.77, p < 0.001, ηp2 = 0.32], but not in the Stroop task [F (1, 58) = 0.61, p = 0.44, ηp2 = 0.01]. Moreover, AB toward SM-related stimuli was found to be positively correlated with the severity of PSMU (r = -0.51, p < 0.001), anxiety (r = -0.37, p < 0.01), depression (r = -0.51, p < 0.001), and social fear (r = -0.30, p < 0.05) in the DPT. CONCLUSIONS: Problematic users of SM show AB towards SM-related cues in the DPT, which is more reliable for assessing AB than the Stroop task. Moreover, it is suggested that in clinical interventions we should work to change the AB towards SM-related stimuli and improve negative emotions to decrease risks of PSMU.

Negative Impacts of Sleep-Wake Rhythm Disturbances on Attention in Young Adults.

Sleep-wake rhythm disturbances have a negative impact on attention. However, how it affects attention and whether the restoration of regular rhythms can restore attention are unclear. This study aims to explore the effects of sleep-wake rhythm disturbances on three subfunctions of attention (alertness, orientation, and executive control) and the restoration of regular rhythms on these functions. Twenty-one participants in the experimental group (who experienced sleep-wake rhythm disturbances for at least one month; aged 18-26) were required to sleep regularly following a sleep schedule, whereas 20 participants in the control group (who maintained regular sleep for at least three months; aged 19-22) received no manipulation of their sleep. All participants were assessed using the attention network test three times in six days. All of them wore spectrum activity monitors and kept sleep diaries every day. The results showed that the effects of alertness and executive control in the experimental group were significantly lower than those in the control group. After five days of regular sleep, the difference in the alertness effect between the two groups significantly decreased. These results suggested that under natural conditions, sleep-wake rhythm disturbances could negatively influence alertness and executive control, and a short period of restoring a regular rhythm has a recovery effect on alertness.

A "Candidatus Liberibacter asiaticus"-secreted polypeptide suppresses plant immune responses in Nicotiana benthamiana and Citrus sinensis.

Citrus Huanglongbing (HLB), known as the most economically devastating disease in citrus industry, is mainly caused by phloem-restricted Gram-negative bacterium "Candidatus Liberibacter asiaticus" (CLas). To date, CLas is still unculturable in vitro, which has been dramatically delaying the research on its pathogenesis, and only few Sec-dependent effectors (SDEs) have been identified to elucidate the pathogenesis of CLas. Here, we confirmed that a CLas-secreted Sec-dependent polypeptide, namely SECP8 (CLIBASIA_05330), localized in nucleus, cytoplasm and cytoplasmic membrane, and showed remarkably higher transcript abundance in citrus than in psyllids. Potato virus X (PVX)-mediated transient expression assays indicated that mSECP8 (the mature form of SECP8) suppressed pro-apoptotic mouse protein BAX and Phytophthora infestans elicitin INF1-triggered hypersensitive response (HR) associated phenotypes, including cell death, H2O2 accumulation and callose deposition. Intriguingly, mSECP8 also inhibited SDE1 (CLIBASIA_05315)-induced water-soaked and dwarfing symptoms in Nicotiana benthamiana. In addition, mSECP8 can promote the susceptibility of transgenic Wanjincheng orange (Citrus sinensis) to CLas invasion and further HLB symptom development, and it contributes to the proliferation of Xanthomonas citri subsp. citri (Xcc). Moreover, the expression of ten immunity-related genes were significantly down-regulated in mSECP8 transgenic citrus than those in wide-type (WT) plants. Overall, we propose that mSECP8 may serve as a novel broad-spectrum suppressor of plant immunity, and provide the first evidence counteractive effect among CLas effectors. This study will enrich and provide new evidences for elucidating the pathogenic mechanisms of CLas in citrus host.

Processing stage flexibility of the SNARC effect: Task relevance or magnitude relevance?

Previous studies have shown that the processing stage of the spatial-numerical association of response codes (SNARC) effect is flexible. Two recent studies used the same experimental paradigm to check whether the SNARC effect occurred in the semantic-representation stage but reached contradictory conclusions, showing that the SNARC effect was influenced by a magnitude Stroop effect in a magnitude comparison task but not by a parity Stroop effect in a parity judgment task. Those two studies had two distinct operational factors: the task type (magnitude comparison task or parity judgment task, with the numerical magnitude information task-relevant or task-irrelevant) and the semantic representation stage-related interference information (magnitude or parity Stroop effect, with the interference information magnitude-relevant or magnitude-irrelevant). To determine which factor influenced the SNARC effect, in the present study, the Stroop effect was switched in the two tasks based on the previous studies. The findings of four experiments consistently showed that the SNARC effect was not influenced by the parity Stroop effect in the magnitude comparison task but was influenced by the magnitude Stroop effect in the parity judgment task. Combined with the results of those two contradictory studies, the findings indicated that regardless of the task type or the task relevance of numerical magnitude information, magnitude-relevant interference information was the primary factor to affect the SNARC effect. Furthermore, a two-stage processing model that explained the observed flexibility of the SNARC effect was proposed and discussed.