Effects of 2.4 GHz radiofrequency electromagnetic field (RF-EMF) on glioblastoma cells (U -118 MG).

INTRODUCTION AND OBJECTIVE: Mobile phones and Wi-Fi are the most commonly used forms of telecommunications. Initiated with the first generation, the mobile telephony is currently in its fifth generation without being screened extensively for any biological effects that it may have on humans or on animals. Some studies indicate that high frequency electromagnetic radiation emitted by mobile phone and Wi-Fi connection can have a negative effect upon human health, and can cause cancer, including brain tumour. OBJECTIVE: The aim of the study was to investigate the influence of 2.4 GHz radiofrequency electromagnetic field (RF-EMF) on the proliferation and morphology of normal (human embryonic kidney cell line Hek-293) and cancer cells (glioblastoma cell line U-118 MG). MATERIAL AND METHODS: The cell cultures were incubated in RF-EMF at the frequency of 2.4 GHz, with or without dielectric screen, for 24, 48 and 72h. In order to analyse the influence of the electromagnetic field on cell lines, Cytotoxicity test Cell Counting Kit-8 was performed. To protect cells against emission of the electromagnetic field, a dielectric screen was used. RESULTS: It was found that 2.4 GHz RF electromagnetic field exposure caused a significant decrease in viability of U-118 MG and Hek-293 cells. The impact of the electromagnetic field was strongest in the case of cancer cells, and the decrease in their survival was much greater compared to the healthy (normal) cells of the Hek-293 line. CONCLUSIONS: Results of the study indicate that using a radio frequency electromagnetic field (2.4 GHz) has a clearly negative effect on the metabolic activity of glioblastoma cells. RF-EMF has much less impact on reducing the viability of normal cells (Hek -293) than cancer cells.

Morphological and cytophysiological changes in selected lines of normal and cancer human cells under the influence of a radio-frequency electromagnetic field.

INTRODUCTION: Currently, mobile phones and Wi-Fi are the most commonly used forms of telecommunication. The popularity of mobile telecommunications has made it necessary to investigate the problem more comprehensively and cautiously assess the possible risks, because never before in history has such a substantial proportion of the population been exposed to microwaves at comparably high levels. Some studies indicate that the high frequency electromagnetic radiation emitted by mobile phone and Wi-Fi connections can have a negative effect on human health, and can cause cancer. OBJECTIVE: The aim of the study was to investigate the influence of the radiofrquency electromagnetic field (RF-EMF) on the metaboloc activity and morphology of normal human cells (fibroblasts) and cancer cells (prostate cancer cells). MATERIAL AND METHODS: The cell cultures (human fibroblasts and prostate cancer cells) were exposed to RF-EMF at the frequency of 2.5 GHz for 24, 48 and 72h. To quantify changes in cell viability, the Cell Counting Kit - 8 was used. RESULTS: It was found that the RF electromagnetic field exposure caused a significant decrease in the viability of fibroblasts, and a significant increase in cancer cells. Morphological analysis did not show significant changes in both cell lines after exposure to RF-EMF. CONCLUSIONS: On the basis of the obtained results, the hypothesis can be formulated that a high frequency electromagnetic field can have harmful effects on human cells.

Effect of low-frequency electric field screening on motility of human sperm.

INTRODUCTION: The human body is constantly exposed to an extremely low electromagnetic field (ELF-EMF), in particular at 50 Hz, emitted by power lines, domestic distribution lines, electrical appliances, etc. It is assumed that the increase in electromagnetic exposure may cause adverse effects upon human health, as well as raising concerns regarding the impact on human fertility. OBJECTIVE: The aim of this in vitro study was to investigate the influence of ELF-EMF with a frequency of 50 Hz on the motility of human sperm. At the same time, the effectiveness of the dielectric screen constructed by ADR Technology ® in absorbing the emitted radiation was examined. MATERIAL AND METHODS: Semen samples of 20 patients were exposed to the influence of an extremely low electromagnetic field. After 5, 15 and 30 min., spermatozoa motility was analysed using a computer-assisted spermatozoa motility analysis system. The following sperm motility parameters were examined: 1) velocity straight linear motility; 2) cross-beat frequency; 3) lateral head displacement; 4) homogeneity of progressive motility velocity. RESULTS: It was found that the ELF-EMF presented a negative effect on the motility of human spermatozoa. A significant decrease in spermatozoa motility speed and a significant increase in lateral head deviation values were observed under the influence of the electromagnetic field. ELF-EMF did not show an effect on either lateral head displacement or homogeneity of progressive motility velocity. CONCLUSIONS: A positive effect of the dielectric screen ADR Technology® was found. This effect compensated spermatozoa motility changes induced with ELF-EMF.