RESUMEN
OBJECTIVE: In this study, we analyzed gene expression levels of apoptotic (Fas, FasL, Bcl-2, Bax) and survival (CXCR1, CXCR2, IL-8) signal pathways of the urine-deprived bladder tissues and the relation of urinary tract infections with these pathways. MATERIAL AND METHODS: We included 37 patients admitted for renal transplantation between December 2009 and December 2012. Bladder mucosal samples were obtained at the time of transplantation and 6-8 weeks posttransplantation, at the time of ureteral catheter removal. RNA extraction and cDNA synthesis were done using guanidium-thiocyanate and colon filter methods. Expression analysis was studied with quantitative real-time polymerase chain reaction optimized with ROX dye and internal control ß-actin. RESULTS: According to our findings Fas, FasL, Bcl-2, and Bax expression was higher in urine-deprived bladder samples than those in the posttransplant samples (P < .05). Although Fas, FasL, Bcl-2, and Bax expression levels increased in pretransplant samples, there was an increase in posttransplant bladder samples; however, this increase was not as marked as those of pretransplant samples. IL-8, CXCR1, and CXCR2 expression was decreased at the pretransplant samples and increased in posttransplant bladder samples. CONCLUSIONS: We have found an upregulated apoptotic process and decreased survival signals at the urine-deprived bladder tissue. After transplantation, bladder tissue survival parameters were increased, demonstrating the importance of urinary flow for bladder tissue.
Asunto(s)
Proteínas Reguladoras de la Apoptosis/metabolismo , Interleucina-8/metabolismo , Trasplante de Riñón , Infecciones Urinarias/metabolismo , Urotelio/metabolismo , Adulto , Apoptosis/fisiología , Proteínas Reguladoras de la Apoptosis/genética , Proteína Ligando Fas/genética , Proteína Ligando Fas/metabolismo , Femenino , Humanos , Interleucina-8/genética , Masculino , Persona de Mediana Edad , Reacción en Cadena en Tiempo Real de la Polimerasa , OrinaRESUMEN
In order to gain the expected benefits from land consolidation projects, the carried out projects are required to provide optimum project conditions. On this account, the success of the project is stated by the aid of various indices. This study aims to present the utility of some metric parameters used in analyzing landscape fragmentation in assessing the land consolidation work. In this study the pre- and post- consolidation conditions of the villages of Serem and Beykoy (in Turkey) were used. In the light of the obtained results, it was concluded that these indicators can be used successfully to determine the success of land consolidation project.
