Ultrasound assessment of gastric contents and volume in patients undergoing endoscopic endonasal transsphenoidal surgery: a prospective observational study.

Intraoperative ingestion of blood, cerebrospinal fluid, and irrigation fluid can lead to an increase in gastric volume, resulting in the potential risk of aspiration in patients after endoscopic endonasal transsphenoidal surgery (EETS). In this prospective observational study, we aimed to assess the volume of gastric contents in patients undergoing this neurosurgical procedure using ultrasound, and to determine the factors associated with volume change. Eighty-two patients diagnosed with pituitary adenoma were recruited consecutively. Semi-quantitative (Perlas scores: 0, 1 and 2) and quantitative (cross-sectional area, CSA) ultrasound assessments of the gastric antrum were performed immediately before and after surgery in the semi-recumbent and semi-recumbent right-lateral positions. Seven (8.5%) patients had antrum scores from preoperative grade 0 to postoperative grade 2; nine (11%) patients had antrum scores from preoperative grade 0 to postoperative grade 1. The mean ± standard deviation (SD) of increased gastric volume was 71.0 ± 33.1 mL and 236.5 ± 32.4 mL in postoperative grade 1 and 2 groups, respectively. Subgroup analysis showed that 11 (13.4%) patients (4 in grade 1 and all in grade 2) had postoperative estimated gastric volume > 1.5 mL kg-1 (mean ± SD 3.08 ± 1.67, range 1.51-5.01 mL kg-1). Logistic regression analysis revealed that older age, diabetes mellitus, and long surgical duration were independent risk factors for significant volume change (all P < 0.05). Our results showed a significant increase in gastric volume in some patients who underwent EETS. Bedside ultrasound measurements of gastric volume can be used to assess the postoperative aspiration risk, particularly in older diabetic patients with a longer surgical duration.

Evidence basis for using dexmedetomidine to enhance the quality of paravertebral block: A systematic review and meta-analysis of randomized controlled trials.

Background: Dexmedetomidine is considered an adjunct to local anaesthesia (LA) to prolong peripheral nerve block time. However, the results from a previous meta-analysis were not sufficient to support its use in paravertebral block (PVB). Therefore, we performed an updated meta-analysis to evaluate the efficacy of dexmedetomidine combined with LA in PVB. Methods: We performed an electronic database search from the date of establishment to April 2022. Randomized controlled trials (RCTs) investigating the combination of dexmedetomidine and LA compared with LA alone for PVB in adult patients were included. Postoperative pain scores, analgesic consumption, and adverse reactions were analyzed. Results: We identified 12 trials (701 patients) and found that the application of dexmedetomidine as a PVB adjunct reduced the postoperative pain severity of patients 12 and 24 h after surgery compared to a control group. Expressed as mean difference (MD) (95% CI), the results were -1.03 (-1.18, -0.88) (p < 0.00001, I2 = 79%) for 12 h and -1.08 (-1.24, -0.92) (p < 0.00001, I2 = 72%) for 24 h. Dexmedetomidine prolonged the duration of analgesia by at least 173.27 min (115.61, 230.93) (p < 0.00001, I2 = 81%) and reduced postoperative oral morphine consumption by 18.01 mg (-22.10, 13.92) (p < 0.00001, I2 = 19%). We also found no statistically significant differences in hemodynamic complications between the two groups. According to the GRADE system, we found that the level of evidence for postoperative pain scores at 12 and 24 h was rated as moderate. Conclusion: Our study shows that dexmedetomidine as an adjunct to LA improves the postoperative pain severity of patients after surgery and prolongs the duration of analgesia in PVB without increasing the incidence of adverse effects.

Fabrication, characterization, and photocatalytic degradation potential of chitosan-conjugated manganese magnetic nano-biocomposite for emerging dye pollutants.

The release of toxic dyes from different industries through industrial effluents cause hazardous effects to human and aquatic life. Therefore, the detoxification of such toxic pollutants is very important for a clean environment. In this regard, chitosan conjugated Mn-magnetic nano-biocomposite (Mn-CCMN) was synthesized, characterized, and applied for the photocatalytic decontamination of crystal violet (CV) dye in the contaminated aqueous system and industrial wastewater. The characterization was performed using SEM, XRD, EDX and FTIR spectroscopic techniques and determine PZC. The data shows the successfully synthesis of the resultant nano-biocomposite with amorphous nature and Fe and Mn were present therein. The SEM study revealed the flat and irregular shaped structure with porous surface and 87 ± 4 nm as mean particle size. The application of as-synthesized nano-biocomposite was determined as a photocatalyst for the eradication of CV in the aqueous environment under UV light illumination. The different photocatalysis conditions were studied for maximum degradation of CV and under the best experimental factors, tremendous CV degradation was attained in the real samples and industrial wastewater. The synthesized Mn-CCMN sustain excellent photocatalytic performance for four successive batches. The photocatalytic data was fitted to the pseudo-first-order kinetic model (R2 = 0.992) having 0.007672 min-1 and 0.0549 ppm/min as k and reaction rate, respectively. The statistical models such as response surface methodology (RSM) and artificial neural network-genetic algorithm (ANN-GA) were also applied for confirmation of the experimental data and the results showed a good agreement. It is deduced that the synthesized Mn-CCMN could be an excellent alternative to the present photocatalysts for the decontamination of toxic organic dyes from contaminated water and industrial wastewater.

Xenobiotics Targeting Cardiolipin Metabolism to Promote Thrombosis in Zebrafish.

Exposure to environmental pollutants is an important factor contributing to the development and severity of thrombosis. However, the important physiological molecules in the thrombotic processes affected by environmental exposures remain unknown. In this study, we show that exposure to environmental chemicals disrupts the equilibrium of cardiolipins (CLs), and directing CL synthesis promotes thrombosis. Using an untargeted metabolomics approach, approximately 3030 molecules were detected in zebrafish embryos exposed to 11 environmental chemicals and automatically clustered into a network. Interconnectivity among CLs and linoleates or isoxanthopterin was discovered through the highly consistent variations in the coregulated metabolites in the network. The chemical exposure resulted in significant upregulation of CLs through influencing the enzymatic activities of phospholipase A2, cardiolipin synthase, and lysocardiolipin acyltransferase. Consequently, metabolic disorders of CLs affected the levels of anticardiolipin antibodies, disrupted the homeostasis between platelet thromboxane A2 and endothelial prostacyclin, and promoted thrombotic events including heart ischemia and tachycardia. Our study thus reveals the common molecular mechanisms underlying the CL-induced thrombosis targeted by environmental exposures.

Visualized Metabolic Disorder and Its Chemical Inducer in Wild Crucian Carp from Taihu Lake, China.

A variety of anthropogenic chemicals can disrupt the equilibrium of intrinsic biological metabolites in organisms, leading to metabolic disorders and an increased risk of metabolic syndromes. However, exposure to pollutants that induce metabolic disorders in wildlife as a cause of adverse effects is unknown. In this study, approximately 3108 compounds, including 11 groups of metabolites and 388 pollutants, were simultaneously identified in the blood of wild crucian carp (Carassius auratus) captured in three bays of Taihu Lake, China. A visualized network linking thousands of co-regulated metabolites was automatically produced for the screened signals. This comprehensive view of the differences in blood metabolite profiles in carp from the north and south bays showed that triglycerides (TGs) were the intrinsic molecules most affected by differing environmental pollution in each bay. The regional differences in metabolite profiles were linked to exposure to screened perfluorinated compounds that displayed corresponding regional differences in concentrations and effects on TGs in in vivo exposure tests. Perfluoroundecanoic acid (PFUnDA) was the key pollutant responsible for the variation in blood TGs in wild crucian carp, and exposure to PFUnDA resulted in extremely high biological activity on lipid deposition in the liver tissues of crucian carp at environmental levels.

Upregulation of lncRNA RMRP promotes the activation of cardiac fibroblasts by regulating miR­613.

Long non­coding RNAs (lncRNAs) have been reported to serve a key role in a variety of cardiovascular diseases, including in cardiac fibrosis. The present study aimed to investigate the biological role and underlying mechanisms of the induction of cardiac fibroblasts by the lncRNA, RNA component of mitochondrial RNA processing endoribonuclease (RMRP). The results demonstrated that RMRP expression was upregulated in the presence of cardiac fibrosis in an abdominal aortic banding­treated rat model. Treatment with angiotensin II increased RMRP expression in cardiac fibroblasts, while the knockdown of RMRP by small­interfering RNA inhibited cardiac fibroblast proliferation, differentiation and collagen accumulation. To further investigate the underlying mechanisms of this interaction, microRNA (miR)­613 was predicted to be a target miR of RMRP and sequence alignment, luciferase activity and MS2 RNA immunoprecipitation were performed to detect the interaction between RMRP and miR­613. The results suggested that RMRP negatively regulated miR­613 in cardiac fibroblasts. Furthermore, miR­613 was indicated to mediate the promoting effect of RMRP on cardiac fibroblast activation. The current study suggested that RMRP promoted cardiac fibroblast activation by acting as a competing endogenous RNA for miR­613. Therefore, RMRP may be a novel target for the prevention or treatment of cardiac fibrosis.

Visualized Networking of Co-Regulated Lipids in Human Blood Based on High-Throughput Screening Data: Implications for Exposure Assessment.

Exposure to environmental chemicals could disturb lipidome homeostasis in biotas. Comprehensive identification and interpretation of lipid molecules in biological samples are of great importance to elucidate the potential changes in lipid homeostasis upon exposure to various environmental stimuli. In this study, a total of 156 human blood samples were collected including 108 general citizens (control group) and 48 employees in a municipal solid waste incineration (MSWI) plant (occupational exposure group). More than 1500 lipid molecules, belonging to five lipid classes, were screened in the blood samples by UPLC-QTOF-MS in the MSE acquisition mode. All of the coupled compounds with correlation coefficients ( R) of 0.7 or higher were selected for automated network correlation analysis. A global visual network was automatically produced from thousands of coregulated lipid species in the blood samples. In the automatically produced molecular network, the distributions of the major correlated lipids were in accordance with their metabolic pathways in the KEGG map. Different lipidomic profiles in the blood samples from the two groups of people were easily observed by this visualization technique. Among the intrinsic lipid classes, glycererides and sterol lipids might represent the most sensitively affected lipids upon exposure to various pollutants emitted from the MSWI plant. The visualized network of coregulated lipids identified in human blood presents a new approach for interpreting the metabolic relationships among the thousands of metabolites identified in toxicological and epidemiological studies.

lncRNA SRA1 Promotes the Activation of Cardiac Myofibroblasts Through Negative Regulation of miR-148b.

Long noncoding RNAs (lncRNAs) have been reported to play a key role in diversity cardiovascular diseases, including cardiac fibrosis. The present study aims to investigate the biological role of lncRNA SRA1 in the activation of cardiac myofibroblasts and the underlying mechanism. Results showed that the expression of SRA1 was upregulated accompanied by cardiac fibrosis in an abdominal aortic banding-treated rat model. Ang-II treatment increased the SRA1 expression of cardiac myofibroblasts, whereas SRA1 knockdown by siRNA inhibited the proliferation, myofibroblast conversion, and collagen production of cardiac myofibroblasts induced by Ang-II. SRA1 overexpression by pcDNA3.1 SRA1-stimulated cardiac myofibroblast activation. To further investigate the underlying mechanism, miR-148b was predicted to be a targeted microRNA of SRA1. Different methods, including sequence alignment, luciferase activity, and MS2 RNA immunoprecipitation were performed to detect the interaction between SRA1 and miR-148b, which suggested that SRA1 negatively regulated miR-148b in cardiac myofibroblasts. Moreover, miR-148b knockdown stimulated cardiac myofibroblast activation, and miR-148b mediated promoting effect of SRA1 on cardiac myofibroblast activation. Collectively, our study suggested that SRA1 promoted cardiac myofibroblast activation by acting as a competitive endogenous RNAs for miR-148b. SRA1 may be a novel potential target for the prevention or therapy of cardiac fibrosis.

Trophodynamics of Emerging Brominated Flame Retardants in the Aquatic Food Web of Lake Taihu: Relationship with Organism Metabolism across Trophic Levels.

Despite the increasing use and discharge of novel brominated flame retardants, little information is available about their trophodynamics in the aquatic food web, and their subsequent relationships to compound metabolism. In this study, concentrations of 2,4,6-tribromophenyl allyl ether (ATE), 1,2-dibromo-4-(1,2-dibromoethyl)cyclohexane (TBECH), tetrabromo- o-chlorotoluene (TBCT), pentabromobenzyl acrylate (PBBA), 1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE), bis(2-ethylhexyl)-3,4,5,6-tetrabromo-phthalate (TBPH), and decabromodiphenyl ethane (DBDPE) were measured in 17 species, including plankton, invertebrates, and fish from Lake Taihu, South China. Trophodynamics of the compounds were assessed, and metabolic rates were measured in the liver microsomes of crucian (trophic level [TL]: 2.93), catfish (TL: 3.86), and yellow-head catfish (TL: 4.3). Significantly positive relationships were found between trophic levels and lipid-normalized concentrations of ATE, BTBPE, and TBPH; their trophic magnification factors (TMFs) were 2.85, 2.83, and 2.42, respectively. Consistently, the three chemicals were resistant to metabolism in all fish microsomes. No significant relationship was observed for ßTBECH ( p = 0.116), and DBDPE underwent trophic dilution in the food web (TMFs = 0.37, p = 0.021). Moreover, these two chemicals showed steady metabolism with incubation time in all fish microsomes. TBCT and PBBA exhibited significant trophic magnifications in the food web (TMF = 4.56, 2.01). Though different metabolic rates were observed for the two compounds among the tested fish species, TBCT and PBBA both showed metabolic resistance in high-trophic-level fish. These results indicated that metabolism of organisms at high trophic levels plays an important role in the assessment of trophic magnification potentials of these flame retardant chemicals.

Screening of Chlorinated Paraffins and Unsaturated Analogues in Commercial Mixtures: Confirmation of Their Occurrences in the Atmosphere.

Characterizing the detailed compositions of chlorinated paraffins (CPs) commercial mixtures is crucial to understand their environmental sources, fates, and potential risks. In this study, dichloromethane (DCM)-enhanced UPLC-ESI-QTOFMS analysis combined with characteristic isotope chlorine peaks is applied to screen all CPs and their structural analogues in the three most commonly produced CP commercial mixtures (CP-42, CP-52, and CP-70). Mass fractions of total short-chain CPs (SCCPs), medium-chain CPs (MCCPs) and long-chain CPs (LCCPs) ranged from 0.64 to 31.9%, 0.64 to 21.8%, and 0.04 to 43.9%, respectively, in the three commercial mixtures. 113 unsaturated SCCPs, MCCPs, and LCCPs were identified in the commercial mixtures. The detailed mass percentages of saturated and unsaturated CPs with carbon numbers of 10-30, chlorine numbers of 5-28, and unsaturated degrees of 0-7 were characterized in all commercial mixtures. Occurrences of the predominant saturated and unsaturated CPs were further confirmed in air samples collected in Guangdong Province, one of the major CP production areas in China, over one year. The profiles of the detected compounds indicated that LCCPs in air samples might come mainly from the production and usage of CP-52, and unsaturated C24-29-LCCPs were specifically originated from CP-70 used in the area.

Trophodynamics of Organic Pollutants in Pelagic and Benthic Food Webs of Lake Dianchi: Importance of Ingested Sediment As Uptake Route.

Habitat is of great importance in determining the trophic transfer of pollutants in freshwater ecosystems; however, the major factors influencing chemical trophodynamics in pelagic and benthic food webs remain unclear. This study investigated the levels of p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE), polycyclic aromatic hydrocarbons (PAHs), and substituted PAHs (s-PAHs) in 2 plankton species, 6 invertebrate species, and 10 fish species collected from Lake Dianchi in southern China. Relatively high concentrations of PAHs and s-PAHs were detected with total concentrations of 11.4-1400 ng/g wet weight (ww) and 5.3-115 ng/g ww, respectively. Stable isotope analysis and stomach content analysis were applied to quantitatively determine the trophic level of individual organisms and discriminate between pelagic and benthic pathways, and the trophodynamics of the detected compounds in the two food webs were assessed. P,p'-DDE was found to exhibit relatively higher trophic magnification rate in the pelagic food web than in the benthic food web. In contrast, PAHs and s-PAHs exhibited greater dilution rates along the trophic levels in the pelagic food web. The lower species differences of pollutants accumulated in benthic organisms compared to pelagic organisms is attributable to extra uptake via ingested sediment in benthos. The average uptake proportions of PAHs and s-PAHs via ingested sediment in benthic biotas were estimated to be 31-77%, and that of p,p'-DDE was 46%. The uptake routes are of importance for assessing the trophic magnification potentials of organic pollutants, especially in eutrophic freshwater ecosystems.

High-Throughput Determination and Characterization of Short-, Medium-, and Long-Chain Chlorinated Paraffins in Human Blood.

The industrial chlorinated paraffins (CPs) are comprised of short-chain (SCCPs), medium chain (MCCPs), and long chain (LCCPs) CPs. Although SCCPs and MCCPs are environmentally ubiquitous, little is known about CPs in humans. This study established a method for simultaneous determination of 261 SCCP, MCCP, and LCCP congener groups in one injection by reversed ultrahigh-pressure liquid chromatography coupled with chlorine-enhanced electron spray ionization-quadrupole time-of-flight mass spectrometry. The method yielded good peak shapes, high sensitivities, and low coeluted interferences for all examined CPs. LCCPs with carbon numbers of 21 to 27 were detected in their standard technical mixtures, and MCCPs and LCCPs impurities were detected in the LCCP and MCCP standard technical mixtures, respectively, causing quantification deviations when these mixtures were used for calibration. After considering these impurities' contribution to the total concentrations, the quantification accuracies for ∑SCCPs, ∑MCCPs, and ∑LCCPs ranged from 95.1 ± 8.4% to 105.6 ± 9.2% in the eight CP technical mixtures. The method was successfully applied to determine CPs in about 6 g human blood samples from a general population, and estimated ∑SCCP, ∑MCCP, and ∑LCCP concentrations to be 370-35 000, 130-3200, and 22-530 ng/g lipid weight (n = 50), respectively. A comparison of blood and soil/air CP profiles from the same areas suggested a relatively higher potential for the accumulation of SCCPs, compared with MCCPs, in humans.

Organobromine compound profiling in human adipose: Assessment of sources of bromophenol.

Bromophenols (BRPs) have been widely detected in human tissues, however, relative proportions from natural products and/or anthropogenic flame retardants are not clear. 21 polybrominated diphenyl ethers (PBDEs), 15 MeO/OH-PBDEs, and 10 BRPs were simultaneously quantified in adipose collected from people from New York City, USA. An in vitro assay utilizing human liver microsomes was performed for detected predominant organobromine. High concentrations of 2,4,6-triBRP and PBDEs were observed, and extremely low concentrations of naturally occurring MeO/OH-PBDEs were detected. Similar biotransformatioin rates of BRPs and MeO/OH-PBDEs indicated that the relative high concentration of 2,4,6-triBRP in humans was not of natural origin. Significant correlation observed between concentrations of 2,4,6-triBRP and BDE-209 suggested that the two chemicals may share a common source. Both 2,4,6-triBRP and BDE-209 were detected in commercial ABS resins, suggesting that plastic products made from ABS resins could be potential sources of co-exposure of the two compounds for humans.