The potential modulation of gut microbiota and oxidative stress by dietary carotenoid pigments.

Gut microbiota plays a crucial role in regulating the response to immune checkpoint therapy, therefore modulation of the microbiome with bioactive molecules like carotenoids might be a very effective strategy to reduce the risk of chronic diseases. This review highlights the bio-functional effect of carotenoids on Gut Microbiota modulation based on a bibliographic search of the different databases. The methodology given in the preferred reporting items for systematic reviews and meta-analyses (PRISMA) has been employed for developing this review using papers published over two decades considering keywords related to carotenoids and gut microbiota. Moreover, studies related to the health-promoting properties of carotenoids and their utilization in the modulation of gut microbiota have been presented. Results showed that there can be quantitative changes in intestinal bacteria as a function of the type of carotenoid. Due to the dependency on several factors, gut microbiota continues to be a broad and complex study subject. Carotenoids are promising in the modulation of Gut Microbiota, which favored the appearance of beneficial bacteria, resulting in the protection of villi and intestinal permeability. In conclusion, it can be stated that carotenoids may help to protect the integrity of the intestinal epithelium from pathogens and activate immune cells.

Gut microbiota plays an essential role in regulating the immune checkpoint therapyCarotenoids are promising molecules in the alteration of gut microbiotaCarotenoids activate the immune cells resulting in a low incidence of oxidative stress.

Plasma-induced nanostructured metallic silver surfaces: study of bacteriophobic effect to avoid bacterial adhesion on medical devices.

Biofilm formation in medical devices represents one of the major problems for the healthcare system, especially those that occur on implantable silicone-based devices. To provide a general solution to avoid biofilm formation in the first stages of development, this work studied how nanostructured metallic silver coatings hinder bacteria-surface interaction by preventing bacteria adhesion. The three studied silver nanostructures ("Sharp blades", "Thick blades" and "Leaves") combined superhydrophobic behavior with a physical impediment of the coating nanostructure that produced a bacteriophobic effect avoiding the adhesion mechanism of different bacterial strains. These silver nanostructures are immobilized on stretchable substrates through a polymeric thin film of plasma-polymerized penta-fluorophenyl methacrylate. The control over the nanostructures and therefore its bacteriophobic-bactericidal effect depends on the plasma polymerization conditions of the polymer. The characterization of this bacteriophobic effect through FE-SEM microscopy, live/dead cell staining, and direct bacterial adhesion counts, provided a complete mapping of how bacteria interact with the surface in each scenario. Results revealed that the bacterial adhesion was reduced by up to six orders of magnitude in comparison with uncoated surfaces thereby constituting an effective strategy to avoid the formation of biofilm on medical materials.

Study of the Human Albumin Role in the Formation of a Bacterial Biofilm on Urinary Devices Using QCM-D.

Catheter-associated urinary tract infections (CAUTIs) are the most common health care-associated infections due to rapid bacterial colonization+ and biofilm formation in urinary catheters. This behavior has been extensively documented in medical devices. However, there is a few literature works on CAUTI providing a model that allows the exhaustive study of biofilm formation in a urinary environment. The development of an effective model would be helpful to identify the factors that promote the biofilm formation and identify strategies to avoid it. In this work, we have developed a model to test biofilm formation on urinary medical device surfaces by simulating environmental and physical conditions using a quartz crystal microbalance with dissipation (QCM-D) module with an uropathogenic strain. Moreover, we used the developed model to study the role of human albumin present in artificial urine at high concentrations because of renal failure or heart-diseases in patients. Despite model limitations using artificial urine, these tests show that human albumin can be considered as a promoter of biofilm formation on hydrophobic surfaces, being a possible risk factor to developing a CAUTI.