RESUMEN
The aim of the present study was to characterize single nucleotide polymorphisms (SNPs) in the promoter region of the bovine paraoxonase 1 (PON1) gene and determine their relationship with PON1 serum activity. Blood samples were collected from 47 Holstein cows during the periparturient period. Serum PON1 was measured and DNA extracted for PCR and sequencing of the promoter region of the PON1 gene. Seven novel SNPs were identified and of these, five SNPs, at positions -105, -221, -392, -611 and -674, upstream of the start of the published mRNA sequence (NM_001046269.2), were found to be associated with variability in serum PON1 activity (P < 0.05). The SNPs at positions -221 and -611 were located in regions predicted to bind to transcription factors linked to the acute phase response.
