Long-Term Bone Mineral Density Changes in Kidney Transplant Recipients Treated with Denosumab: A Retrospective Study with Nonequivalent Control Group.

Data on the effectiveness of denosumab on osteoporosis after kidney transplantation are limited. We investigated the long-term bone mineral density (BMD) changes in kidney transplant recipients (KTRs) treated with denosumab compared to untreated KTRs. We enrolled KTRs treated with denosumab 60 mg/6 months for 4 years. An untreated group of sex and age-matched KTRs with a 1:1 ratio was included. The primary outcome was BMD changes assessed by Dual-energy X-ray Absorptiometry over 4 years. Data on serum creatinine, alkaline phosphatase (ALP), parathyroid hormone, and 25-hydroxyvitamin D were collected. All patients received oral cholecalciferol and calcium supplementation. 23 denosumab-treated KTRs were enrolled, and 23 untreated KTRs. The median time from transplant to the start of denosumab was 4 years (range 0:24). The denosumab group showed a significant increase from baseline in BMD at the lumbar spine (LS) (9.0 ± 10.7%, p < 0.001), and total hip (TH) (3.8 ± 7.9%, p = 0.041). The untreated group showed a significant decrease at all sites (- 3.0 ± 7%, p = 0.041 at the LS; - 6.3 ± 9.2%, p = 0.003 at the TH; - 6.7 ± 9.3%, p = 0.003 at the FN). The between-group differences in percent BMD changes were statistically significant at all sites. Similar results were found for the respective Z-scores. The ALP serum levels significantly decreased from baseline only in the denosumab group, with a significant between-group difference (p = 0.032). No significant differences in serum creatinine, hypocalcaemic events or acute graft rejection rates were observed. Four years of denosumab therapy were associated with increased BMD in KTRs, while untreated KTRs showed significant BMD losses at all sites.

The effects of prefrontal tDCS and hf-tRNS on the processing of positive and negative emotions evoked by video clips in first- and third-person.

The causal role of the cerebral hemispheres in positive and negative emotion processing remains uncertain. The Right Hemisphere Hypothesis proposes right hemispheric superiority for all emotions, while the Valence Hypothesis suggests the left/right hemisphere's primary involvement in positive/negative emotions, respectively. To address this, emotional video clips were presented during dorsolateral prefrontal cortex (DLPFC) electrical stimulation, incorporating a comparison of tDCS and high frequency tRNS stimulation techniques and manipulating perspective-taking (first-person vs third-person Point of View, POV). Four stimulation conditions were applied while participants were asked to rate emotional video valence: anodal/cathodal tDCS to the left/right DLPFC, reverse configuration (anodal/cathodal on the right/left DLPFC), bilateral hf-tRNS, and sham (control condition). Results revealed significant interactions between stimulation setup, emotional valence, and POV, implicating the DLPFC in emotions and perspective-taking. The right hemisphere played a crucial role in both positive and negative valence, supporting the Right Hemisphere Hypothesis. However, the complex interactions between the brain hemispheres and valence also supported the Valence Hypothesis. Both stimulation techniques (tDCS and tRNS) significantly modulated results. These findings support both hypotheses regarding hemispheric involvement in emotions, underscore the utility of video stimuli, and emphasize the importance of perspective-taking in this field, which is often overlooked.

Risk of fracture in women with glucocorticoid requiring diseases is independent from glucocorticoid use: An analysis on a nation-wide database.

OBJECTIVE: Glucocorticoid-induced osteoporosis (GIOP) is a common cause of secondary osteoporosis. However, glucocorticoid requiring diseases pose a risk themselves for fracture. The aim of the present study was to determine the risk of fracture associated with variety of glucocorticoid requiring diseases independently from glucocorticoid use and other risk factors for osteoporosis. METHODS: We conducted a retrospective cross-sectional analysis of a nation-wide cohort (DeFRACalc79 database). We used multivariable regression analysis adjusting for several risk factors for fracture and glucocorticoid intake to estimate the independent role of glucocorticoid requiring illnesses on fracture risk. RESULTS: We found that patients with rheumatoid arthritis, connective tissue diseases, chronic obstructive pulmonary disease (COPD) and neurological diseases were at greater risk of vertebral or hip fracture (crude ORs 1.31, 1.20, 1.92 and 2.97 respectively). After adjusting for potential confounders COPD and neurological diseases remained significantly associated with an increased risk of vertebral or hip fractures (aORs 1.33, 95 % CI 1.18-1.49 and 2.43, 95 % CI 2.17-2.74). Rheumatoid arthritis, COPD, IBD and neurological diseases also significantly increased the risk of non-vertebral, non-hip fractures (aORs 1.23, 1.42, 1.52 and 1.94 respectively). CONCLUSION: Some glucocorticoid requiring diseases were independently associated with an increased risk of fractures. COPD and neurological diseases with both vertebral and non-vertebral fracture risk while RA and IBD were independently associated only with non-vertebral, non-hip fractures.

Changing Decisions: The Interaction between Framing and Decoy Effects.

BACKGROUND: Cognitive biases are popular topics in psychology and marketing, as they refer to systematic cognitive tendencies in human thinking that deviate from logical and rational reasoning. The framing effect (FE) and the decoy effect (DE) are examples of cognitive biases that can influence decision making and consumer preferences. The FE involves how options are presented, while the DE involves the addition of a third option that influences the choice between the other two options. METHODS: We investigated the interaction between the FE and the DE in the case of both incongruent (ID) and congruent (CD) decoys in a sample of undergraduates (n = 471). The study had a two (positive vs. negative valence) × three (original, congruent decoy, incongruent decoy) within-subject design. RESULTS: The ID option reduces the FE in both positive- and negative-framed conditions compared to the controls, while adding the CD option increases the FE only in the positive-framed condition. Additionally, the inclusion of the CD option enhances the level of decision confidence, whereas no significant differences were found in the ID condition. CONCLUSIONS: Our findings gave new insights into the interplay between two of the most frequent cognitive biases.

Summer pruning in Mediterranean vineyards: is climate change affecting its perception, modalities, and effects?

Summer pruning encompasses a series of operations typically performed on the grapevine during the growing season. This review provides an update on the research conducted over the last 20 years on the modalities and strategies of main summer pruning operations, which include shoot positioning and thinning, shoot trimming, leaf removal, and cluster thinning, with a special focus on their adaptation to climate change occurring in Mediterranean areas. Three main novelties emerged from the survey. First, due to a common need to shelter clusters against overheating and sunburn-related damages, shoot thinning and leaf removal are practices that are now being applied in a much more cautious and conservative manner. Second, the meaning of summer pruning is evolving because operations are being used as precious tools to direct ripening toward a desired direction rather than being received passively. Third, some operations, such as leaf removal, have disclosed very high plasticity, which means that, depending on the timing and modalities of the intervention, yield can be either increased or decreased and ripening anticipated or postponed. In an era where economic and environmental sustainability have to find a good compromise, cluster thinning is increasingly being depicted as an extraordinary operation that should be left to occasional occurrences of overcropping. Moreover, summer pruning is a tool through which growers can, to an extent, exploit the potentialities offered by climate change. For instance, the crop-forcing technique, under the different configurations of single and double cropping within the same season, has been trialed promisingly in several regions and cultivars. The principle of forcing is to unlock the dormant bud during the first year by removing at least the young organs present on the shoot within a time window between the end of the flowering and pea-size stages. In particular, when it is applied in a double-cropping mode, the preliminary results related to Pinot noir, Grenache, Tempranillo, and Maturana tinta indicate that two harvests separated by 30-50 days can be obtained, with the latter having superior quality in terms of a lower level of pH and higher levels of acidity, anthocyanins, and phenolics.

With or without the Mask: Age Differences in Perceived Trustworthiness and Health during the COVID-19 Pandemic.

The COVID-19 pandemic and the obligation to wear surgical face masks have affected social interactions. Wearing a mask can cause impairments in face identification, emotion recognition, and trait impressions. The present study investigated, during the COVID-19 period, age-related differences in perceived trustworthiness (Study 1) and health (Study 2) when viewing faces with or without masks. Younger (YAs, 18-35 years) and older (OAs, over 65 years) adults' ratings were compared. Through a web-based platform, a series of neutral younger and older faces (YFs vs. OFs) were presented, on a computer screen, with or without a mask (Mask vs. No-Mask), and participants were asked to rate them on a 7-point scale. Furthermore, data collected during the pandemic (Mask and No-Mask conditions) were compared with ratings obtained before it (Pre-COVID condition). Perceived trustworthiness was lower in the No-Mask condition for both age groups compared to Mask and Pre-COVID conditions, which did not differ. For health ratings, no differences emerged for OAs between the conditions, whereas YAs' ratings were lower in both the Mask and No-Mask conditions compared to the Pre-COVID condition. The fear of contracting COVID-19 affected both trustworthiness and health ratings. Wearing a surgical face mask affects trait impressions for YAs and OAs, partly due to the fear of COVID-19. Trait impressions are also influenced by the age of the face to be evaluated.

A comparative study of fifteen cover crop species for orchard soil management: water uptake, root density traits and soil aggregate stability.

Increasing the use of cover crops (CCs) is a necessity in sustainable viticulture, although it might clash with possible excessive competition towards vines. Especially in a climate-change scenario, the latter feature should be minimized while maintaining ecosystem services. Aimed at identifying CCs for vineyard floor management, the trial characterized several species according to their evapotranspiration (ET) rates, root growth patterns, and soil aggregate stability potential. The study was performed in 2020 in Piacenza (Northern Italy) on 15 CC species grown in pots kept outdoor and classified as grasses (GR), legumes (LE) and creeping (CR). Together with bare soil (control), they were arranged in a complete randomized block design. CCs ET was assessed through a gravimetric method, starting before mowing and then repeated 2, 8, 17 and 25 days thereafter. Above-ground dry biomass (ADW), root length density (RLD), root dry weight (RDW) and root diameter class length (DCL) were measured, and mean weight diameter (MWD) was calculated within 0-20 cm depth. Before mowing, ET was the highest in LE (18.6 mm day-1) and the lowest in CR (8.1 mm day-1) the latter being even lower than the control (8.5 mm day-1). The high ET rates shown by LE were mainly related to very fast development after sowing, rather than to a higher transpiration per unit of leaf area. After mowing, the 15 species' ET reduction (%) plotted vs leaf area index (LAI, m2 m-2) yielded a very close fit (R2 = 0.94), suggesting that (i) a linear decrease in water use is expected anytime starting with an initial LAI of 5-6, (ii) a saturation effect seems to be reached beyond this limit. Selection of cover crop species to be used in the vineyard was mainly based on diurnal and seasonal water use rates as well as dynamic and extent of root growth patterns. Among GR, Festuca ovina stood out as the one with the lowest ET due to its "dwarfing" characteristics, making it suitable for a permanent inter-row covering. CR species confirmed their potential for under-vine grassing, assuring rapid soil coverage, lowest ET rates, and shallow root colonization.

Radiofrequency echographic multi-spectrometry and DXA for the evaluation of bone mineral density in a peritoneal dialysis setting.

BACKGROUND: The aim of this real-life cross-sectional explorative study was to compare radiofrequency echographic multi-spectrometry (REMS) with dual-energy X-rays absorptiometry (DXA) in the BMD assessment of patients receiving peritoneal dialysis (PD). Furthermore, we investigated the relationship between lumbar aortic calcifications (AOCs) and the DXA lumbar measurements. METHODS: Consecutive patients referring to the PD clinic of our hospital were included. Lumbar spine and femur scans were acquired with both techniques (including lumbar laterolateral DXA scans). The risk assessment of two fracture risk algorithms (FRAX® and DeFRA®) were compared. Cohen's k coefficients were used to assess the inter-technique agreement in the classification of patients as osteoporotic. Lumbar AOCs were estimated semi-quantitatively on laterolateral DXA scans. RESULTS: 41 patients were enrolled. No significant differences were documented between the BMD T-scores measured through DXA or REMS at the femur. At the lumbar spine, the DXA anteroposterior mean T-score (- 0.49 ± 1.98) was significantly higher than both the laterolateral DXA (- 1.66 ± 0.99) and the REMS (- 2.00 ± 1.94) measurements (p < 0.01 vs both). No significant differences were found between the DXA and REMS fracture risk estimates with both algorithms. The inter-technique Cohen's k coefficient (for the worst T-score, any site) was 0.421, p < 0.001. The discrepancy between the DXA laterolateral and anteroposterior lumbar T-score was positively associated with the AOCs extent and severity (r = 0.402, p < 0.01). CONCLUSIONS: Our data showed a promising agreement, in a real-life PD setting, between DXA and REMS BMD assessment and in the consequent fracture risk estimation and confirm the AOCs interference on the diagnostic accuracy of lumbar DXA.

UAV-Based Hyperspectral Monitoring Using Push-Broom and Snapshot Sensors: A Multisite Assessment for Precision Viticulture Applications.

Hyperspectral aerial imagery is becoming increasingly available due to both technology evolution and a somewhat affordable price tag. However, selecting a proper UAV + hyperspectral sensor combo to use in specific contexts is still challenging and lacks proper documental support. While selecting an UAV is more straightforward as it mostly relates with sensor compatibility, autonomy, reliability and cost, a hyperspectral sensor has much more to be considered. This note provides an assessment of two hyperspectral sensors (push-broom and snapshot) regarding practicality and suitability, within a precision viticulture context. The aim is to provide researchers, agronomists, winegrowers and UAV pilots with dependable data collection protocols and methods, enabling them to achieve faster processing techniques and helping to integrate multiple data sources. Furthermore, both the benefits and drawbacks of using each technology within a precision viticulture context are also highlighted. Hyperspectral sensors, UAVs, flight operations, and the processing methodology for each imaging type' datasets are presented through a qualitative and quantitative analysis. For this purpose, four vineyards in two countries were selected as case studies. This supports the extrapolation of both advantages and issues related with the two types of hyperspectral sensors used, in different contexts. Sensors' performance was compared through the evaluation of field operations complexity, processing time and qualitative accuracy of the results, namely the quality of the generated hyperspectral mosaics. The results shown an overall excellent geometrical quality, with no distortions or overlapping faults for both technologies, using the proposed mosaicking process and reconstruction. By resorting to the multi-site assessment, the qualitative and quantitative exchange of information throughout the UAV hyperspectral community is facilitated. In addition, all the major benefits and drawbacks of each hyperspectral sensor regarding its operation and data features are identified. Lastly, the operational complexity in the context of precision agriculture is also presented.

The Protective Role of Neurogenetic Components in Reducing Stress-Related Effects during Spaceflights: Evidence from the Age-Related Positive Memory Approach.

Fighting stress-related effects during spaceflight is crucial for a successful mission. Emotional, motivational, and cognitive mechanisms have already been shown to be involved in the decrease of negative emotions. However, emerging evidence is pointing to a neurogenetic profile that may render some individuals more prone than others to focusing on positive information in memory and increasing affective health. The relevance for adaptation to the space environment and the interaction with other stressors such as ionizing radiations is discussed. In particular, to clarify this approach better, we will draw from the psychology and aging literature data. Subsequently, we report on studies on candidate genes for sensitivity to positive memories. We review work on the following candidate genes that may be crucial in adaptation mechanisms: ADRA2B, COMT, 5HTTLPR, CB1, and TOMM40. The final aim is to show how the study of genetics and cell biology of positive memory can help us to reveal the underlying bottom-up pathways to also increasing positive effects during a space mission.

Simulating Extreme Environmental Conditions via Mental Imagery: The Case of Microgravity and Weight Estimation.

Mental imagery can be used for recreating an extreme environment experience. Here we assessed whether microgravity effects over cognition, that typically occur during a space mission, may be reproduced via mental imagery. Participants were randomly assigned to one of two conditions in which they were guided to imagine to be (1) in outer space or (2) in a nature scenario and subsequently estimate the weight of common objects. We found that only for those who engaged in a space scenario imagery, there was a decrease in object weight estimation compared with a prior rating. This finding is the first to indicate that the effects of weightlessness on cognition can be simulated via an imagery-based technique and add to the ongoing debate about the importance of trying to disentangle the effect of microgravity alone on human performance. Moreover, our findings ultimately suggest that imagery can be used as a less expensive simulated scenario for studying the impact of extreme environmental conditions over astronauts' cognition and behavior.

Assessing Grapevine Biophysical Parameters From Unmanned Aerial Vehicles Hyperspectral Imagery.

Over the last 50 years, many approaches for extracting plant key parameters from remotely sensed data have been developed, especially in the last decade with the spread of unmanned aerial vehicles (UAVs) in agriculture. Multispectral sensors are very useful for the elaboration of common vegetation indices (VIs), however, the spectral accuracy and range may not be enough. In this scenario, hyperspectral (HS) technologies are gaining particular attention thanks to the highest spectral resolution, which allows deep characterization of vegetative/soil response. Literature presents few papers encompassing UAV-based HS applications in vineyard, a challenging conditions respect to other crops due to high presence of bare soil, grass cover, shadows and high heterogeneity canopy structure with different leaf inclination. The purpose of this paper is to present the first contribution combining traditional and multivariate HS data elaboration techniques, supported by strong ground truthing of vine ecophysiological, vegetative and productive variables. Firstly the research describes the UAV image acquisition and processing workflow to generate a 50 bands HS orthomosaic of a study vineyard. Subsequently, the spectral data extracted from 60 sample vines were elaborated both investigating the relationship between traditional narrowband VIs and grapevine traits. Then, multivariate calibration models were built using a double approach based on Partial Least Square (PLS) regression and interval-PLS (iPLS), to evaluate the correlation performance between the biophysical parameters and HS imagery using the whole spectral range and a selection of more relevant bands applying a variable selection algorithm, respectively. All techniques (VIs, PLS and iPLS) provided satisfactory correlation performances for the ecophysiological (R 2 = 0.65), productive (R 2 = 0.48), and qualitative (R 2 = 0.63) grape parameters. The novelty of this work is represented by the first assessment of a UAV HS dataset with the expression of the entire vine ecosystem, from the physiological and vegetative state to grapes production and quality, using narrowband VIs and multivariate PLS regressions. A correct non-destructive estimation of key parameters in vineyard, above all physiological parameters which must be measured in a short time as they are extremely influenced by the variability of environmental conditions during the day, represents a powerful tool to support the winegrower in vineyard management.

Affective health and countermeasures in long-duration space exploration.

Background: Space research is shifting attention toward interplanetary expeditions. Therefore, whether long-duration spaceflight may influence affective health is becoming an urgent issue. Method: To this end, we undertook a literature search and reviewed several behavioral simulation studies on Earth that focused on affective components in space. We concluded with studies showing how spaceflight can impact on affective health of astronauts with a positively laden trajectory. Results: By analyzing the multifaceted theoretical concept of affective health, we show that there is a variety of affective states (e.g., stress, coping, adaptation, and resilience) that can be differently affected by spaceflight. Conclusions: Countermeasures geared toward promoting positive emotions could play a key role in positive adaptation to extreme environments and thus during long-duration space missions may benefit. Subjective resilience plays a mediating role in adaptation, but its definition needs to be deepened in order to develop robust countermeasures that may prevent the onset of emotional disorders.

"When did you see it?" The effect of emotional valence on temporal source memory in aging.

Previous studies consistently showed age-related differences in temporal judgment and temporal memory. Importantly, emotional valence plays a crucial role in older adults' information processing. In this study, we examined the effects of emotions at the intersection between time and memory, analysing age-related differences in a temporal source memory task. Twenty-five younger adults (age range 18-35), 25 old adults (age range 65-74), and 25 old-old adults (age range 75-84) saw a series of emotional pictures in three sessions separated by a one-day rest period. In the fourth session, participants were asked to indicate in which session (1, 2, or 3) they saw each picture. Results showed that old-old adults tended to collocate negative pictures distant in time, while positive stimuli were remembered as more recent than real, compared to neutral pictures. To a lower extent, people over 65 showed the same pattern of results. In contrast, emotional valence did not affect younger adults' temporal positioning of stimuli. Current findings fit well with the Socio-Emotional Selectivity Theory's assumptions and extended the literature on the positivity effect to temporal source memory.

Pharmacodynamics of Oral Cholecalciferol in Healthy Individuals with Vitamin D Deficiency: A Randomized Open-Label Study.

Comparative pharmacodynamic (PD) analyses on different dosing schedules for cholecalciferol supplementation are limited. This was an open-label, randomized, parallel-group study involving 75 healthy individuals deficient in vitamin D (baseline 25OHD < 20 ng/mL) receiving oral cholecalciferol with three different dosing regimens: Group A: 10,000 IU/day for 8 weeks followed by 1000 IU/day for 4 weeks; Group B: 50,000 IU/week for 12 weeks and Group C: 100,000 IU every other week for 12 weeks. Regulators of calcium and phosphate homeostasis, bone turnover markers and Wnt inhibitors were measured at baseline, Day 28, 53, 84, and 112. The 1,25OH2D increased at each time point. The increase was greater (p < 0.05) for group A vs. B and C at Day 28, and vs. group B at Day 56. No significant difference among groups was observed for the other biomarkers. The 24,25OH2D remained stable over time. PTH decreased at Day 84 and FGF-23 increased at all time points. CTX-I and PINP increased slightly at Day 28. BALP decreased from Day 56 onward. Dkk-1 increased from Day 56 onward, while sclerostin did not show significant changes. In healthy individuals deficient in vitamin D, vitamin D supplementation exerted effects on multiple regulators of calcium, phosphate and bone metabolism, without marked differences using the three regimens.

Subpicosecond metamagnetic phase transition in FeRh driven by non-equilibrium electron dynamics.

Femtosecond light-induced phase transitions between different macroscopic orders provide the possibility to tune the functional properties of condensed matter on ultrafast timescales. In first-order phase transitions, transient non-equilibrium phases and inherent phase coexistence often preclude non-ambiguous detection of transition precursors and their temporal onset. Here, we present a study combining time-resolved photoelectron spectroscopy and ab-initio electron dynamics calculations elucidating the transient subpicosecond processes governing the photoinduced generation of ferromagnetic order in antiferromagnetic FeRh. The transient photoemission spectra are accounted for by assuming that not only the occupation of electronic states is modified during the photoexcitation process. Instead, the photo-generated non-thermal distribution of electrons modifies the electronic band structure. The ferromagnetic phase of FeRh, characterized by a minority band near the Fermi energy, is established 350 ± 30 fs after the laser excitation. Ab-initio calculations indicate that the phase transition is initiated by a photoinduced Rh-to-Fe charge transfer.

Gas exchange, vine performance and modulation of secondary metabolism in Vitis vinifera L. cv Barbera following long-term nitrogen deficit.

MAIN CONCLUSION: A reprogramming of secondary metabolism to acclimate to nitrogen deficiency was seen in grapevine eliciting an accumulation of strigolactones and jasmonate. This response links with photosynthetic compensation and enhanced ripening. In addition to the metabolism directly related to nitrogen assimilation, long-term nitrogen depletion may affect plant secondary metabolism, in turn affecting grapevine performance. In this work, the effect of nitrogen deficit was investigated in V. vinifera cv. Barbera potted vines following three years of deprivation, using a combination of morpho-physiological assessments and mass spectrometry-based untargeted metabolomics. Plants grown under nitrogen limitation showed reduced growth and even more curtailed yields, lowered SPAD values, and a quite preserved leaf gas exchange, compared to plants grown under non-limiting nitrogen availability. Ripening was decidedly accelerated, and berry composition improved in terms of higher sugar and phenolic contents under nitrogen-limiting conditions. Metabolomics showed the broad involvement of secondary metabolism in acclimation to nitrogen deficiency, including a distinctive modulation of the phytohormone profile. Several nitrogen-containing metabolites were down accumulated under nitrogen-limiting conditions, including alkaloids, glucosinolates, hypoxanthine, and inosine. On the other hand, phenylpropanoids showed an accumulation trend. Concerning the recruitment of hormones, nitrogen deprivation elicited an accumulation of strigolactones and jasmonate. Noteworthy, both strigolactones and jasmonates have been previously related to increased photosynthetic efficiency under abiotic stress. Furthermore, the severe reduction of lateral shoot development we recorded in N-deprived vines is consistent with the accumulation of strigolactones. Overall, our results suggest that nitrogen deprivation induced a rather broad metabolic reprogramming, mainly including secondary metabolism and hormones profile, reflected in the modulation of photosynthetic performance, canopy growth, and possibly fruit quality.