1.
Biochemistry (Mosc)
; 72(1): 21-8, 2007 Jan.
Artículo
en Inglés
| MEDLINE
| ID: mdl-17309433
RESUMEN
Substrate specificity of Escherichia coli thymidine phosphorylase to thymidine derivatives modified at 5' -, 3' -, and 2' ,3' - positions of the sugar moiety was studied. Equilibrium and kinetic constants (K(m), K(I), k(cat)) of the phosphorolysis reaction have been determined for 20 thymidine analogs. The results are compared with X-ray and molecular dynamics data. The most important hydrogen bonds in the enzyme-substrate complex are revealed.