RESUMEN
The comparative analysis of the rat liver and blood serum creatine kinase, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and purine nucleoside phosphorylase post-radiation activity levels after a total two-hour long single and fractional exposure of the animals to low-intensity 900 MHz frequency electromagnetic field showed that the most sensitive enzymes to the both schedules of radiation are the liver creatine kinase, as well as the blood serum creatine kinase and alkaline phosphatase. According to the comparative analysis of the dynamics of changes in the activity level of the liver and blood serum creatine kinase, alanine aminotransferase, aspartate aminotransferase and purine nucleoside phosphorylase, both single and fractional radiation schedules do not affect the permeability of a hepatocyte cell membrane, but rather cause changes in their energetic metabolism. The correlation analysis of the post-radiation activity level changes of the investigated enzymes did not reveal a clear relationship between them. The dynamics of post-radiation changes in the activity of investigated enzyme levels following a single and short-term fractional schedules of radiation did not differ essentially.
Asunto(s)
Teléfono Celular , Hepatocitos/enzimología , Hígado/enzimología , Alanina Transaminasa/sangre , Fosfatasa Alcalina/sangre , Animales , Aspartato Aminotransferasas/sangre , Creatina Quinasa/sangre , Radiación Electromagnética , Hepatocitos/efectos de la radiación , Hígado/efectos de la radiación , Purina-Nucleósido Fosforilasa/sangre , RatasRESUMEN
The effects of a single exposure of rats to the whole-body roentgen irradiation at the doses of 3.5 Gy and 4.5 Gy on the activity of creatine kinase, purine nucleoside phosphorylase, alanine aminotransferase, aspartate aminotransferase, as well as on the state of the nuclear-nucleolar apparatus in rat hepatocytes on the 6th and 13th days after radiation exposure have been studied. Irradiation at the above doses induced changes in the levels of enzymatic activity of different values and different directions within the same time periods, as well as oscillating changes in this type of enzymatic activity over time. This demonstrates various radiosensitivity and adaptation abilities of these enzymatic activities. The changes in the enzymatic activity significantly correspond to the changes in the morphometric indices of nuclear-nucleolar apparatus of hepatocytes, as well as the distribution of hepatocytes within the ploidy classes: in particular, stabilization of the enzymatic activity on the 13th day after irradiation correlates with the increased transcriptional activity, which is detectable through the increased number of nucleoli per nucleus and the expanded space of a hepatocyte nucleus. The compensation mechanisms are likely to be targeted at the changes in the functional activity of surviving hepatocytes, rather than at the replacement of the damaged cells by the new ones.
Asunto(s)
Nucléolo Celular , Hepatocitos , Hígado , Radiación Ionizante , Alanina Transaminasa/metabolismo , Animales , Aspartato Aminotransferasas/metabolismo , Nucléolo Celular/enzimología , Nucléolo Celular/efectos de la radiación , Creatina Quinasa/metabolismo , Hepatocitos/enzimología , Hepatocitos/efectos de la radiación , Hígado/enzimología , Hígado/efectos de la radiación , Masculino , Ploidias , Purina-Nucleósido Fosforilasa/metabolismo , Ratas , Irradiación Corporal TotalRESUMEN
Purine nucleoside phosphorylase (PNP) catalyzes reversible phosphorolysis of purine deoxy- and ribonucleosides with formation (d)Rib-1-P and corresponding bases. PNP plays a leading role in the cell metabolism of nucleosides and nucleotides, as well as in maintaining the immune status of an organism. The major aim of the majority of studies on the PNP is the detection of highly effective inhibitors of this enzyme, derivatives ofpurine nucleosides used in medicine as immunosuppressors, which are essential for creating selective T-cell immunodeficiency in a human body for organ and tissue transplantation. The present work is devoted to the study of the effects of some synthetic derivatives of purine nucleosides on activity of highly purified PNP from rabbit spleen and also from human healthy and tumor tissues of lung and kidneys. Purine nucleoside analogues modified at various positions of both the heterocyclic base and carbohydrate residues have been investigated. Several compounds, including 8-mercapto-acyclovir, 8-bromo-9-(3,4-hydroxy-butyl)guanine, which demonstrated potent PNP inhibition, could be offered for subsequent study as immunosuppressors during organ and tissue transplantation.
Asunto(s)
Inhibidores Enzimáticos/farmacología , Guanosina/farmacología , Inosina/farmacología , Purina-Nucleósido Fosforilasa/antagonistas & inhibidores , Adenosina/análogos & derivados , Adenosina/farmacología , Animales , Guanosina/análogos & derivados , Humanos , Inosina/análogos & derivados , Riñón/enzimología , Pulmón/enzimología , Neoplasias/enzimología , Purina-Nucleósido Fosforilasa/metabolismo , ConejosRESUMEN
PNP catalyzes a reversible phosphorolysis of purine deoxy- and ribonucleosides with formation of (d)Rib-1-P and appropriate bases. PNP plays a leading role in the cell metabolism of nucleosides and nucleotides, as well as in maintaining the immune status of an organism. The major purpose of the majority of studies on the PNP is the detection of high-performance enzyme inhibitors, derivatives of the purine nucleosides, which are used in medicine as immunosuppressors. It is well known that the latter are necessary for creating a selective T-cell immunodeficiency in a human body under organs and tissue transplantation. The review discusses the issues related to deliberate synthesis of effective, metabolically inert, and low-toxic PNP inhibitors. It also analyzes the available studies on substrate and inhibitory properties of the analogues of purine nucleosides, as well as research on the structural factors which reinforce the inhibitor activity of those analogues. The inhibitors which are either used in medical practice or are currently at a stage of preclinical testing are described. The inhibitors which are more efficient in their influence on the PNF from tumorous tissues are of special interest. Using PNP inhibitors in case of a number of pathologies denotes the importance and promise of research on both the enzyme and the compounds affecting its activity.
Asunto(s)
Inhibidores Enzimáticos/uso terapéutico , Inmunosupresores/uso terapéutico , Purina-Nucleósido Fosforilasa/antagonistas & inhibidores , Animales , Biomarcadores/sangre , Inhibidores Enzimáticos/farmacología , Humanos , Síndromes de Inmunodeficiencia/tratamiento farmacológico , Síndromes de Inmunodeficiencia/inmunología , Inmunosupresores/farmacología , Purina-Nucleósido Fosforilasa/sangre , Purina-Nucleósido Fosforilasa/deficiencia , Purina-Nucleósido Fosforilasa/fisiología , Purinas/metabolismo , Especificidad por Sustrato , Linfocitos T/efectos de los fármacos , Linfocitos T/inmunologíaRESUMEN
The influence of carboxylic group modification with N-cyclohexyl-N'-beta-(4-methyl-morpholine)ethylcarbodiimide on the activity of creatine kinase was examined. The modification rate for M- and M'-subunits of the enzyme depends on the reagent concentration and the presence of Mg2+ ions. The process is described by linear dependences of logarithms of activity vs. time, indicating a pseudofirst order of reactions. The reagent inactivates M- and M'-subunits of the enzyme at approximately the same rate, modification being characterized by rate constants of 0.17 min-1 and K1 of 0.17 M. Mild alkaline hydrolysis (pH 9.2) of the modified enzyme leads to partial (30-60%) restoration of its activity. The addition of [14C]glycine methyl ester results in the irreversible incorporation of radioactivity into the protein. AMP and ATP gamma-(p-azido-anilide), the inhibitors acting competitively to nucleotide substrates, protect the enzyme against inactivation with the reagent, whereas creatine and creatine phosphate exert no influence on the modification rate of the enzyme. It is suggested that modification affects the aspartate or glutamate carboxyls at or near the ATP-binding sites in the M- and M'-subunits of the enzyme.
Asunto(s)
Creatina Quinasa/metabolismo , Músculos/enzimología , Animales , Sitios de Unión , CME-Carbodiimida/farmacología , Ácidos Carboxílicos , Fenómenos Químicos , Química , Creatina Quinasa/antagonistas & inhibidores , Indicadores y Reactivos , Cinética , ConejosRESUMEN
Creatine kinase from skeletal muscle (EC 2.7.3.2) was inactivated by means of imidazolides of AMP, ADP, ATP. Rates of the inactivation of the enzyme's M- and M'-subunits differ 50-100 fold and decrease in the presence of ADP and ATP. Differential spectrum of the native and modified enzymes corresponds to the spectrum of N,O-diacetyltyrosine. Kinetic curves of hydroxylamine-dependent destruction of N,O-diacetyltyrosine and of alteration of differential spectrum of the modified and native enzymes essentially coincide. The enzyme's inactivation appears to be caused mainly by the formation of a bond between nucleotide imidazolides activated carboxyl group of the active centre and OH-group of Tyr residue arranged in the close proximity. The stoichiometry of acyltyrosine formation is evaluated as 2.1 +/- 0.2 mole per mole of the functional dimer. Along with formation of ester bond between amino acid residues, a covalent attachment of 0.03-0.06 mole of [14C]nucleotides per mole of enzyme is observed. As the data of acid hydrolysis show, Im-ATP and Im-AMP block epsilon-amino group of Lys and guanidine group of Arg, respectively. Reasons of the multiple modification of creatine kinase by affinity reagents are discussed. The results obtained and literature data are summarised in the hypothetical scheme of disposition of various amino acid residues in the active centre of creatine kinase.
Asunto(s)
Nucleótidos de Adenina/farmacología , Creatina Quinasa/metabolismo , Imidazoles/farmacología , Músculos/enzimología , Tirosina/análogos & derivados , Marcadores de Afinidad , Animales , Sitios de Unión , Ácidos Carboxílicos , Creatina Quinasa/antagonistas & inhibidores , Activación Enzimática , Cinética , Conejos , Tirosina/metabolismoRESUMEN
A study of creatine kinase modification by ATP gamma-(N-(2-chloroethyl)-N-methyl)amide was performed. The attachment 1,7-1,8 moles of analogue per mole of functional dimer results in full inactivation of the enzyme. The substrates, ATP and ADP, protect the enzyme both against inactivation and covalent binding of analogue. The affinity modification rate depends on the reagent and magnesium ion concentrations and pH of the reaction mixture. The dissociation constants (1,0 and 1,5 mM) for the enzyme-analogue complexes and the affinity modification maximal rate constants (2,1 X 10(-3) and 1,2 X 10(-3) c-1) in the absence and presence of Mg2+ ions were estimated. Some differences in the affinity modification rates were observed for the nonidentical M and M'-subunits of creatine kinase. The data obtained are indicative of a histidine residue alkylation by the ATP analogue. This histidine (pK 7,7) may function as a general acid-base catalyst in deprotonation of the guanidinium group of creatine as the latter is phosphorylated by ATP.
Asunto(s)
Adenosina Trifosfato/análogos & derivados , Creatina Quinasa/antagonistas & inhibidores , Músculos/enzimología , Compuestos de Mostaza Nitrogenada/farmacología , Adenosina Trifosfato/metabolismo , Adenosina Trifosfato/farmacología , Marcadores de Afinidad , Animales , Sitios de Unión , Fenómenos Químicos , Química , Creatina Quinasa/metabolismo , Activación Enzimática , Técnicas In Vitro , Conformación Proteica , Conejos , Especificidad por SustratoRESUMEN
Periodate-oxidized ADP and ATP (oADP and oATP) are substrates and affinity reagents for creatine kinase from rabbit skeletal muscle. oADP and oATP modified a lysine epsilon-amino group in the nucleotide-binding site of the enzyme. Complete inactivation is observed upon binding 2 moles oADP per 1 mole of the enzyme dimer. Modification with oADP is described by a liner dependence of the log of enzyme activity on time, testifying to a pseudo-first-order of the reaction. The reaction rate constant (ki = 8.10(3) min-1) and dissociation constant for the reversible enzyme-oADP complex (Kd = 62 microM) were determined. ADP protected the enzyme from inactivation and covalent binding of the analog, whereas oADP covalently bound to the enzyme was phosphorylated by phosphocreatine. The data obtained allow to suggest that the epsilon-amino group of a lysine residue of the active site is located in close proximity to ribose of ATP and ADP forming a complex with the enzyme. This group seems essential for correct orientation of the nucleotide polyphosphate chain in the enzyme active center, but take no immediate part in the transphosphorylation process.
Asunto(s)
Adenosina Difosfato/metabolismo , Adenosina Trifosfato/metabolismo , Creatina Quinasa/metabolismo , Músculos/enzimología , Animales , Cromatografía de Afinidad , Cinética , Conejos , Especificidad por SustratoRESUMEN
Ultraviolet irradiation of creatine kinase (adenosine 5'-triphosphate: creatine-N-phosphotransferase, EC 2.7.3.2) in the presence of gamma-(p-azidoanilide)-[14C]-ATP results in a complete enzyme inactivation and covalent binding of two moles of analog per mole of enzyme. MgADP strongly protects the enzyme against inactivation. However, in the presence of MgADP the covalent binding of 0.9 moles of analog per mole of enzyme occurs without the enzyme inactivation. The rate of enzyme inactivation and covalent modification decreases in the presence of MgATP and creatine. These results indicate that gamma-(p-azidoanilide)-ATP is an affinity label for the active site of creatine kinase.