RESUMEN
During an outbreak of Wellfleet Bay virus (WFBV) in common eiders ( Somateria mollissima) from the Cape Cod region of Massachusetts, several birds were diagnosed with trichomonosis consisting of multiple trichomonad species. Six birds were examined, with trichomonads found in ceca in four birds and associated typhlitis in three of these four birds. PCR and DNA sequencing utilizing trichomonad-specific primers targeting the ITS1 region of the ribosomal DNA (rDNA) revealed the presence of Tetratrichomonas gallinarum in the gastrointestinal tracts of five birds and Trichomonas spp. in the livers of two birds, one of which had a dual Te. gallinarum-Trichomonas gallinae infection. Sequence analysis revealed no variation between Te. gallinarum sequences whereas the ITS1 sequences obtained from the other Trichomonas spp. demonstrated the presence of multiple genotypes. One sequence had 100% identity to a Trichomonas sp. previously isolated from a Cooper's hawk ( Accipiter cooperii) and the other sequence was 100% identical to a previously described Tr. gallinae isolate obtained from a Pacific Coast band-tailed pigeon ( Patagioenas fasciata monilis). These findings suggest Te. gallinarum and other Trichomonas spp. possibly contributed to morbidity and mortality in this species. Furthermore, to the authors' knowledge, this is the first report of trichomonad-associated disease in a free-ranging sea duck.
Asunto(s)
Enfermedades de las Aves/parasitología , Patos , Infecciones Protozoarias en Animales/parasitología , Trichomonadida/aislamiento & purificación , Tricomoniasis/veterinaria , Animales , Animales Salvajes , ADN Protozoario/análisis , ADN Ribosómico/análisis , Femenino , Genotipo , Masculino , Massachusetts , Trichomonadida/genética , Trichomonas/genética , Trichomonas/aislamiento & purificación , Tricomoniasis/parasitologíaRESUMEN
There are reports of horses with acute onset acquired cervical scoliosis and cutaneous analgesia. The underlying dorsal gray column myelitis that produces these neurologic signs has been only presumptively attributed to migration of Parelaphostrongylus tenuis within the spinal cord. Despite previous confirmation brain by polymerase chain reaction testing, of P. tenuis within the brain of horses by polymerase chain reaction testing, genetic testing has failed to definitively identify the presence of this parasite in cases of equine myelitis. This case report provides molecular confirmation via polymerase chain reaction of P. tenuis within the cervical spinal cord of a horse with scoliosis and cutaneous analgesia.
Asunto(s)
Infecciones del Sistema Nervioso Central/veterinaria , Enfermedades de los Caballos/parasitología , Meningitis/veterinaria , Metastrongyloidea , Mielitis/veterinaria , Escoliosis/veterinaria , Infecciones por Strongylida/veterinaria , Animales , Infecciones del Sistema Nervioso Central/complicaciones , Infecciones del Sistema Nervioso Central/diagnóstico , Infecciones del Sistema Nervioso Central/parasitología , Femenino , Enfermedades de los Caballos/diagnóstico , Caballos , Meningitis/diagnóstico , Meningitis/parasitología , Mielitis/diagnóstico , Mielitis/parasitología , Reacción en Cadena de la Polimerasa/veterinaria , Escoliosis/etiología , Escoliosis/parasitología , Infecciones por Strongylida/complicaciones , Infecciones por Strongylida/parasitologíaRESUMEN
Field isolates of coccidia from 20 natural outbreaks in the chukar partridge ( Alectoris chukar ) were received from gamebird farms in 10 U.S. states. These were propagated in the laboratory and identified by microscopy and PCR. Of 20 samples, 18 were Eimeria kofoidi, two were Eimeria legionensis only, and one was a mixture of the two species. One isolate of E. kofoidi also contained an unidentified species detected only by PCR, nucleotide sequencing, and phylogenetic analysis. The efficacy of anticoccidial drugs against chukar coccidia was tested with experimental infections in battery cages. Isolates of E. kofoidi were used to infect 2-wk-old chukars. Anticoccidial products were given in the feed at levels approved for other poultry or for chukars. Tests were terminated at 6 days postinoculation with measurement of weight gains, fecal diarrhea scores, and necropsy to observe for lesion severity. Lasalocid (120 ppm) was moderately effective in one test. When tested against four field isolates, other ionophores (monensin, salinomycin, semduramicin) showed moderate effectiveness in reducing lesions and improving weight gains. Rofenaid (a potentiated sulfa mixture), robenidine (30 ppm), diclazuril (2 ppm), and decoquinate (80 ppm) were highly effective. In a test of nine products against a highly virulent field isolate, only diclazuril (2 ppm) and clopidol (125 ppm) reduced the severity of lesions and improved weight gain relative to infected controls, suggesting the extent to which previous drug usage had selected for drug resistance.
Asunto(s)
Enfermedades de las Aves/tratamiento farmacológico , Coccidiosis/tratamiento farmacológico , Coccidiosis/veterinaria , Coccidiostáticos/administración & dosificación , Eimeria/efectos de los fármacos , Galliformes/parasitología , Animales , Enfermedades de las Aves/epidemiología , Enfermedades de las Aves/parasitología , Coccidiosis/epidemiología , Coccidiosis/parasitología , Brotes de Enfermedades , Eimeria/genética , Eimeria/aislamiento & purificación , Eimeria/fisiología , Filogenia , Estados Unidos/epidemiologíaRESUMEN
A new method to amplify coccidia DNA by polymerase chain reaction (PCR) was developed by placing freeze-thawed oocysts in Ready-to-Go PCR bead tubes and using a 5-min initial heat denaturation step. Positive PCR reactions were found in 3 of 3 samples containing 20 or 50 oocysts; when ≤5 oocysts were used, 1 of 3 samples was positive. This technique shows potential for effectively and efficiently detecting and identifying oocysts from soil, feces, and other matter.
Asunto(s)
ADN Protozoario/aislamiento & purificación , Eimeria/genética , Eimeria/aislamiento & purificación , Heces/parasitología , Reacción en Cadena de la Polimerasa/métodos , Suelo/parasitología , Animales , Enfermedades de las Aves/diagnóstico , Enfermedades de las Aves/parasitología , Aves , Coccidiosis/diagnóstico , Coccidiosis/parasitología , ADN Protozoario/química , Microesferas , Oocistos/químicaRESUMEN
The white-tailed deer (Odocoileus virginianus) is a common and widespread North American game species. To evaluate the incidence, clinical manifestations, demography, and pathology of bacterial and parasitic dermatologic diseases in white-tailed deer in the southeastern United States, we retrospectively evaluated white-tailed deer cases submitted to the Southeastern Cooperative Wildlife Disease Study from 1975 to 2012. Among 2569 deer examined, bacterial or parasitic dermatologic disease was diagnosed in 88 (3.4%) individuals, with Demodex spp (n = 37; 42.0%) and Dermatophilus congolensis (n = 19; 21.6%) as the most common causes. Demodicosis was significantly more common in deer older than 2 years and was most often detected in the fall; no statistically significant sex predilection was identified. Affected animals had patchy to generalized alopecia, often distributed over the head, neck, limbs, and trunk; microscopic lesions included epidermal crusts and cutaneous nodules with mild perifollicular, lymphoplasmacytic inflammation. Dermatophilosis was most common in males younger than 1 year that were often found dead. Crusting, erythema, and alopecia occurred on the face, ears, and distal extremities. Less commonly, infectious dermatologic diseases were associated with other bacteria (n = 13; 14.8%), fungi (n = 5; 5.7%), ectoparasites (chiggers, lice, mites, and ticks; n = 11; 12.5%), and larval nematodes (n = 7; 8.0%). Population-level effects of these diseases in white-tailed deer are likely minimal; however, due to their dramatic presentation, demodicosis, dermatophilosis, and other infectious skin diseases can be of concern to hunters and, in some cases, may have zoonotic potential.
Asunto(s)
Infecciones por Actinomycetales/veterinaria , Ciervos/microbiología , Ciervos/parasitología , Infestaciones por Ácaros/veterinaria , Infecciones por Actinomycetales/epidemiología , Infecciones por Actinomycetales/patología , Factores de Edad , Alopecia/veterinaria , Animales , Eritema/veterinaria , Historia del Siglo XX , Historia del Siglo XXI , Incidencia , Infestaciones por Ácaros/epidemiología , Infestaciones por Ácaros/patología , Sudeste de Estados Unidos/epidemiologíaRESUMEN
Free-roaming cat populations have been identified as a significant public health threat and are a source for several zoonotic diseases including rabies, toxoplasmosis, cutaneous larval migrans because of various nematode parasites, plague, tularemia and murine typhus. Several of these diseases are reported to cause mortality in humans and can cause other important health issues including abortion, blindness, pruritic skin rashes and other various symptoms. A recent case of rabies in a young girl from California that likely was transmitted by a free-roaming cat underscores that free-roaming cats can be a source of zoonotic diseases. Increased attention has been placed on trap-neuter-release (TNR) programmes as a viable tool to manage cat populations. However, some studies have shown that TNR leads to increased immigration of unneutered cats into neutered populations as well as increased kitten survival in neutered groups. These compensatory mechanisms in neutered groups leading to increased kitten survival and immigration would confound rabies vaccination campaigns and produce naïve populations of cats that can serve as source of zoonotic disease agents owing to lack of immunity. This manuscript is a review of the various diseases of free-roaming cats and the public health implications associated with the cat populations.
Asunto(s)
Animales Salvajes , Enfermedades de los Gatos/transmisión , Rabia/veterinaria , Toxoplasmosis Animal/transmisión , Zoonosis/transmisión , Animales , Castración , Enfermedades de los Gatos/parasitología , Enfermedades de los Gatos/virología , Gatos , Humanos , Larva Migrans/transmisión , Larva Migrans/veterinaria , Regulación de la Población , Salud Pública , Rabia/transmisiónRESUMEN
An adult male guinea pig (Cavia porcellus) with a 1-month history of hind limb paresis, torticollis, and seizures was euthanized and submitted for necropsy. Gross examination was unremarkable, but histologic examination revealed multifocal eosinophilic and lymphoplasmacytic choriomeningitis and cross sections of nematode parasites within the leptomeninges of the midbrain and diencephalon. Morphologic features of the nematode were consistent with a metastrongyle, and the parasite was identified as Parelaphostrongylus tenuis by polymerase chain reaction testing and nucleotide sequencing. Further questioning of the owner revealed that the guinea pig was fed grass from a yard often grazed by white-tailed deer (Odocoileus virginianus). To the authors' knowledge, this is the first report of a naturally occurring P. tenuis infection in a guinea pig.
Asunto(s)
Ciervos/parasitología , Cobayas/parasitología , Meningitis/veterinaria , Metastrongyloidea/aislamiento & purificación , Enfermedades de los Roedores/parasitología , Infecciones por Strongylida/veterinaria , Animales , Diagnóstico Diferencial , Masculino , Meninges/parasitología , Meninges/patología , Meningitis/parasitología , Meningitis/patología , Metastrongyloidea/genética , Paresia/veterinaria , Poaceae/parasitología , Enfermedades de los Roedores/patología , Convulsiones/veterinaria , Infecciones por Strongylida/parasitología , Infecciones por Strongylida/patología , Tortícolis/veterinariaRESUMEN
We report the first documented occurrence of an outbreak of trichomonosis in a free-ranging small flock of Eurasian collared doves (Streptopelia decaocto) and African collared dove hybrids (Streptopelia risoria) in the Caribbean. In total, 18 birds were examined, including six African collared dove x Eurasian collared dove hybrids and 12 Eurasian collared doves. The affected age class consisted of adults. Sex distribution was equal. With a flock population size of 200 birds, mortality rate for the outbreak was estimated at 15-20%. Living birds were weak, showing evidence of mucus-stained beaks and open-mouth breathing. Caseous ulcerative yellow lesions were restricted to the upper gastrointestinal tract, with the exception of one bird, which had lesions in the upper gastrointestinal tract and in the liver. Ninety-four percent (17/18) of the affected birds had multiple extensive lesions. Lesions located on the roof of the oral cavity extended in 33% (6/18) into the orbit and in 11% (2/18) into the braincase. Using wet-mount microscopy, we were able to confirm Trichomonas gallinae in 22% (4/18) of the sampled animals. Fifteen samples submitted for PCR analysis tested positive. Sequence analysis of the internal transcribed spacer 1 (ITS-1) region of the ribosomal RNA (rRNA) revealed two distinct genotypes of Trichomonas. One sequence had 100% identity to the prototype T. gallinae isolate, whereas the other sequences had 98-100% identity to recently described Trichomonas-like parabasalid. On the basis of gross and histologic findings, along with the sequence results from the columbids in this report, it is likely that this Trichomonas-like parabasalid is pathogenic.
Asunto(s)
Enfermedades de las Aves/parasitología , Columbidae , Parabasalidea/aislamiento & purificación , Tricomoniasis/veterinaria , Trichomonas/aislamiento & purificación , Animales , Enfermedades de las Aves/epidemiología , Enfermedades de las Aves/patología , Región del Caribe/epidemiología , ADN Espaciador Ribosómico/genética , Femenino , Genotipo , Masculino , Parabasalidea/clasificación , Parabasalidea/genética , Reacción en Cadena de la Polimerasa/veterinaria , Trichomonas/clasificación , Trichomonas/genética , Tricomoniasis/epidemiología , Tricomoniasis/parasitología , Tricomoniasis/patologíaRESUMEN
Experiments were conducted to determine whether chukar partridge (Alectoris chukar) chicks would develop protective immunity after inoculation with coccidia. Young chukar chicks in battery cages inoculated with 100 or more oocysts of Eimeria kofoidi or Eimeria legionensis had significant protection at challenge 4 wk later, as measured by greatly reduced oocyst shedding and improved weight gain as compared with unvaccinated, challenged controls. However, when birds were housed in litter pens and vaccinated by various regimens (including two species of chukar coccidia at 100/dose), coccidiosis rapidly spread through all treatments and caused significant mortality. Vaccination with Coccivac-T or with 100 oocysts of Eimeria dispersa did not prevent mortality resulting from accidental contamination, and feed treatment with a Lactobacillus competitive-exclusion product had no benefit. Most if not all of the mortality was from E. kofoidi. This study illustrated the natural fecundity of chukar coccidia in a floor-pen environment where multiplication rate and reinfection combine to produce clinical disease from a small original exposure. Further, these results cast doubt on the potential use of low doses of live oocysts as a vaccine in the chukar partridge.
Asunto(s)
Coccidiosis/veterinaria , Eimeria/fisiología , Galliformes , Enfermedades de las Aves de Corral/prevención & control , Vacunas Antiprotozoos/inmunología , Vacunación/veterinaria , Animales , Coccidiosis/inmunología , Coccidiosis/prevención & control , Eimeria/crecimiento & desarrollo , Lactobacillus , Oocistos/fisiología , Enfermedades de las Aves de Corral/inmunología , Vacunas Antiprotozoos/administración & dosificación , Especificidad de la EspecieRESUMEN
To determine whether chemotherapeutic compounds available for use in domestic poultry are effective at controlling coccidiosis in northern bobwhites (Colinus virginianus), we tested 13 chemotherapeutic anticoccidials including amprolium (250 parts per million [ppm]), clopidol (125 ppm), diclazuril (1 ppm and 2 ppm), decoquinate (30 ppm), lasalocid (120 ppm), monensin (90 ppm), narasin/nicarbazin (36/36 ppm), robenidine (33 ppm), roxarsone (50 ppm), sulfadimethoxine/ ormetoprin (125/75 ppm), salinomycin (60 ppm), semduramicin (25 ppm), and zoalene (125 ppm and 150 ppm). Three tests were conducted using two replicates of 10 birds each: Infected, unmedicated controls and medicated birds were challenged with 1 x 10(6) oocysts of a field isolate consisting primarily of Eimeria lettyae. Subsequently, we tested clopidol, lasalocid, salinomycin, diclazuril (1 ppm), and monensin against mixed-species field isolates containing E. lettyae, E. dispersa, E. colini, or all. Weight gain, gross intestinal lesions, severity of diarrhea, and feed conversion ratio (FCR) 6 days postinfection were recorded. Lesion score, as previously reported, was unreliable as a measure of severity of infection in comparison with weight gain, fecal scores, and FCR. Excellent to good efficacy was found in clopidol, decoquinate, diclazuril (1 ppm and 2 ppm), and in lasalocid, narasin and nicarbazin, robenidine, sulfadimethoxine/ormetoprin, and zoalene (150 ppm). Marginal protection was found using monensin, salinomycin, semduramicin, or a roxarsone/semduramicin combination. Amprolium, roxarsone, and zoalene (125 ppm) were ineffective at controlling coccidia. Two of the six isolates tested against diclazuril 1 ppm and clopidol demonstrated a high degree of resistance, but none of the six isolates was resistant to lasalocid. Four of the eight isolates showed mild to moderate, and moderate to high, resistance against monensin and salinomycin, respectively. These findings indicate that several available compounds are effective at controlling coccidiosis in bobwhites.
Asunto(s)
Coccidiosis/veterinaria , Coccidiostáticos/uso terapéutico , Enfermedades de las Aves de Corral/prevención & control , Codorniz , Alimentación Animal , Animales , Coccidiosis/prevención & control , Eimeria , Metabolismo Energético , Aumento de Peso/efectos de los fármacosRESUMEN
An outbreak of coccidiosis in laboratory-reared Chinese ring-necked pheasants (Phasianus colchicus) resulted in high morbidity and moderate mortality. The outbreak was associated with a breach in biosecurity caused by the cleaning of a sewer line with a mechanical device, resulting in extensive splattering of fecal material throughout the "clean room" where birds were held prior to use in coccidiosis experiments. Mortality and morbidity in the affected birds were seen exactly 5 days after the incident, after birds had been moved to another room for experimental use, corresponding closely with the known prepatent or preclinical period of Eimeria phasiani and Eimeria colchici. Gross lesions in the affected birds varied from dehydration to intestinal and ventricular hemorrhage. Microscopic examination confirmed a diagnosis of severe intestinal coccidiosis. This report underscores the ease of contamination of experimental birds leading to coccidiosis outbreaks during breaches of management and biosecurity.
Asunto(s)
Animales de Laboratorio , Enfermedades de las Aves/parasitología , Coccidiosis/veterinaria , Galliformes , Crianza de Animales Domésticos , Animales , Enfermedades de las Aves/transmisión , Coccidiosis/transmisión , Vivienda para AnimalesRESUMEN
A moribund 5-year-old female northern river otter (Lontra canadensis) was found on the bank of a river known to be extensively contaminated with mercury. It exhibited severe ataxia and scleral injection, made no attempt to flee, and died shortly thereafter of drowning. Tissue mercury levels were among the highest ever reported for a free-living terrestrial mammal: kidney, 353 microg/g; liver, 221 microg/g; muscle, 121 microg/g; brain (three replicates from cerebellum), 142, 151, 151 microg/g (all dry weights); and fur, 183 ug/g (fresh weight). Histopathologic findings including severe, diffuse, chronic glomerulosclerosis and moderate interstitial fibrosis were the presumptive cause of clinical signs and death. This is one of a few reports to document the death of a free-living mammal from presumed mercury poisoning.
Asunto(s)
Intoxicación por Mercurio/veterinaria , Nutrias , Animales , Animales Salvajes , Resultado Fatal , Femenino , Intoxicación por Mercurio/mortalidad , Intoxicación por Mercurio/patologíaRESUMEN
Fresh ceca samples from turkeys in North Carolina infected with Histomonas meleagridis were collected at necropsy, inoculated into warmed Dwyers medium, and sent by overnight courier to our laboratory at The University of Georgia. Further incubation at 40 C yielded positive cultures from all four samples. PCR and DNA sequencing confirmed the presence of H. meleagridis. To further establish conditions for survival in transit, we infected turkeys with H. meleagridis, euthanatized the birds 10 days postinfection, and allowed carcasses to incubate at room temperature for either 2 or 24 hr. After incubation, samples of cecal contents (0.5 g) were placed in Dwyers medium and held at 4, 25, or 30 C for 6, 18, 24, 48, 72, 96, or 120 hr, simulating holding conditions during transit. Samples were placed in a 40 C incubator at the specified times and examined daily for histomonad growth by light microscopy. Positive histomonad growth was detected from cecal samples obtained from the 2-hr incubated carcass and from cultures held at 30 C for 6, 18, 24, 48, and 72 hr. No growth was seen from cultures held at 25 or 4 C or at any temperature from the carcass allowed to incubate for 24 hr at room temperature. These results suggest that positive isolation can be made from field samples, provided that material is collected at warm temperatures and transported rapidly to the laboratory.
Asunto(s)
Técnicas de Cultivo/veterinaria , Enfermedades de las Aves de Corral/parasitología , Infecciones Protozoarias en Animales/parasitología , Trichomonadida/fisiología , Pavos , Animales , Enfermedades de las Aves de Corral/diagnóstico , Infecciones Protozoarias en Animales/diagnóstico , Manejo de EspecímenesAsunto(s)
Encefalitis/veterinaria , Enfermedades de los Caballos/patología , Metastrongyloidea/genética , Infecciones por Strongylida/veterinaria , Animales , Secuencia de Bases , ADN de Helmintos/química , ADN de Helmintos/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Encefalitis/microbiología , Encefalitis/patología , Enfermedades de los Caballos/microbiología , Caballos , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa/veterinaria , Análisis de Secuencia de ADN , Infecciones por Strongylida/microbiología , Infecciones por Strongylida/patologíaRESUMEN
To determine whether northern bobwhite quail (Colinus virginianus) could be immunized against Eimeria lettyae by a low-dose inoculation of oocysts, we inoculated 30 birds each with either 100 or 1000 oocysts at 2 days of age (given orally by pipette). Four weeks after immunization, the immunized birds and unimmunized controls were challenged with 1 x 10(6) E. lettyae oocysts. Eight days after challenge, birds were killed and weighed, and their intestines examined for gross lesions. Effectiveness of the immunization was measured by analyzing weight gain, intestinal lesions, severity of diarrhea, feed conversion ratio, and oocyst production. After challenge, birds immunized with 100 or 1000 oocysts gained an average of 33.3 g and 28.9 g, respectively, whereas unimmunized challenged birds gained an average of 11.5 g. Immunized quail produced approximately 99.7% fewer oocysts, had minimal gross intestinal and cecal lesions, had minimal diarrhea, and had a 50% lower feed conversion ratio compared to unimmunized challenged controls. These findings indicate that vaccination is a viable option for controlling coccidiosis in quail and that further research into vaccination is warranted.
Asunto(s)
Coccidiosis/veterinaria , Eimeria/inmunología , Enfermedades de las Aves de Corral/prevención & control , Vacunas Antiprotozoos/inmunología , Animales , Coccidiosis/prevención & control , Colinus , Relación Dosis-Respuesta Inmunológica , PavosRESUMEN
The value of wildlife has been widely ignored or under-rated in the past by the international community. At most, wildlife was considered from the limited aesthetic and touristic aspects. This situation has changed somewhat. In the majority of the veterinary profession, which is largely livestock-oriented, wildlife is increasingly considered in terms of wild animal production and occupies just as relevant a position as domestic animal production. Some economists are now trying to quantify the informal nature of a large portion of the wildlife sector. The importance of wildlife to local communities is now globally recognised in community-based or participatory natural resources management programmes. The authors highlight not only the economic importance of wildlife (which amounts to billions of United States dollars world-wide), through consumptive and non-consumptive uses, but also the present and potential nutritional value, the ecological role as well as the socio-cultural significance of wildlife for human societies of both the developed and the developing worlds. Also addressed in this chapter is a discussion on one of the main threats to wildlife conservation which consists of the reduction or even retrieval of the different values wildlife can offer.
Asunto(s)
Animales Salvajes , Valor de la Vida , Crianza de Animales Domésticos/economía , Animales , Animales Salvajes/fisiología , Conservación de los Recursos Naturales/economía , Países Desarrollados/economía , Países en Desarrollo/economía , Ecosistema , Abastecimiento de Alimentos/economía , Humanos , Carne/economía , Carne/normas , Valor Nutritivo , Valor de la Vida/economíaRESUMEN
Over a period of a year, samples of water, foam, microbial mat, soil and air were obtained from areas associated with the cooling canal of a nuclear power station. The seventeen sample sites included water in the cooling canal that was thermally enriched and soil and water adjacent to, upstream, downstream and at a distance from the generator. Air samples were taken at the plant and at various distances from the plant. Fifty-two species of thermotolerant and thermophilic fungi were isolated. Of these, eleven species are grouped as opportunistic Mucorales or opportunistic Aspergillus sp. One veterinary pathogen was also isolated (Dactylaria gallopava). The opportunistic/pathogenic fungi were found primarily in the intake bay, the discharge bay and the cooling canal. Smaller numbers were obtained at both upstream and downstream locations. Soil samples near the cooling canal reflected an enrichment of thermophilous organisms, the previously mentioned opportunistic Mucorales and Aspergillus spp. Their numbers were found to be greater than that usually encountered in a mesophilic environment. However, air and soil samples taken at various distances from the power station indicated no greater abundance of these thermophilous fungi than would be expected from a thermal enriched environment. Our results indicate that there was no significant dissemination of thermophilous fungi from the thermal enriched effluents to the adjacent environment. These findings are consistent with the results of other investigators.