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The intrinsic nature of CRISPR-Cas in conferring immunity to bacteria and archaea has been repurposed to combat pathogenic agents in mammalian and plant cells. In this regard, CRISPR-Cas13 systems have proved their remarkable potential for single-strand RNA viruses targeting. Here, different types of Cas13 orthologs were applied to knockdown foot-and-mouth disease virus (FMDV), a highly contagious disease of a wide variety of species with genetically diverse strains and is widely geographically distributed. Using programmable CRISPR RNAs capable of targeting conserved regions of the viral genome, all Cas13s from CRISPR system type VI (subtype A/B/D) could comprehensively target and repress different serotypes of FMDV virus. This approach has the potential to destroy all strains of a virus as targets the ultra-conserved regions of genome. We experimentally compared the silencing efficiency of CRISPR and RNAi by designing the most effective short hairpin RNAs according to our developed scoring system and observed comparable results. This study showed successful usage of various Cas13 enzymes for suppression of FMDV, which provides a flexible strategy to battle with other animal infectious RNA viruses, an underdeveloped field in the biotechnology scope.
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Crimean-Congo hemorrhagic fever [CCHF] is a severe infectious viral disease caused by a tick borne virus which can lead to fatal hemorrhagic disease in humans. It has been reported from some continents including Africa, Asia and Europe. Virus is transmitted to human mainly through tick bite, whose acquire infection from reservoirs wild and domesticated mammalians and ostriches. Currently no approved vaccine or drug is available for CCHF and prevention is mainly based on biosecurity measures. Ribavirin is the only approved drug that has been used in some countries to treat human disease, however some new studies did not prove the Ribavirin efficacy. Different strategies to design effective vaccines, have been conducted through years, from inactivated virus to nucleotide-based ones including DNA and mRNA vaccines. In this study we review of pioneering vaccine candidate platforms.
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Avian encephalomyelitis (AE) is an important infectious poultry disease worldwide that is caused by avian encephalomyelitis virus (AEV). The causative virus can be transmitted both horizontally and vertically. In the present study, an AEV suspected outbreak with typical neurological signs occurred in broilers. Histopathological examination, RT-PCR assay and full genome sequencing were applied to confirm the presence of AEV. Phylogenetic analysis of the full genome sequence showed that the detected AEV strain at 7055 nucleotide length is classified in cluster I and is closely related to vaccinal USA and China originated isolates. Although, the outbreaks of AE in progeny of vaccinated breeders have been reported previously, the source of infection was unknown. Based on the results obtained in this study, the outbreaks are vaccine-originated. This study provides the first whole genome analysis of AEV from Iran and reveals that the AEV possesses a hepatitis C virus-like internal ribosome entry site.
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Pollos , Virus de la Encefalomielitis Aviar , Genoma Viral , Filogenia , Enfermedades de las Aves de Corral , Animales , Irán , Genoma Viral/genética , Enfermedades de las Aves de Corral/virología , Enfermedades de las Aves de Corral/transmisión , Pollos/virología , Virus de la Encefalomielitis Aviar/genética , Transmisión Vertical de Enfermedad Infecciosa , Brotes de Enfermedades , Secuenciación Completa del Genoma , Infecciones por Avulavirus/virología , Infecciones por Avulavirus/veterinariaRESUMEN
The whole genomic sequence of fowl adenovirus C (FAdV-4) strain FAdV-4/Pasouk, isolated from chickens with hepatitis-hydropericardium syndrome (HHS) from an outbreak in Iran, has been deposited in GenBank under accession number ON652872. Notably, this FAdV-4 isolate exhibited significant genetic similarities to contemporary isolates originating from China, indicating a shared ancestry.
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Newcastle disease virus (NDV) belongs to the Avulavirus genus and Paramyxoviridae family virus that causes acute, highly infectious Newcastle disease in poultry. The two proteins of haemagglutinin neuraminidase (HN) and fusion (F) are key virulence factors with an important role in its immunogenicity. Genotype VII NDV is still among the most serious viral hazards to the poultry industry worldwide. In this study, a commercial vector vaccine (HVT-NDV) was evaluated compared to the conventional vaccination strategy against Iranian genotype VII. This experiment showed that the group receiving the conventional vaccination strategy had higher antibodies, fewer clinical signs, and lower viral loads in tracheal swabs and feces. Also, two vaccine groups showed significant difference, which could have resulted from two extra vaccine doses in the conventional group. However, except for antibody levels in commercial chickens in the Iran new-generation vaccine, this difference was minor. Further, both groups showed 100% protection in the challenge study. Despite the phylogenetic gap between the NDV-F gene placed in the vector vaccine and the challenge virus (genotypes I and VII, respectively), the rHVT-NDV vaccine offered strong clinical protection and decreased challenge virus shedding considerably.
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Enfermedad de Newcastle , Enfermedades de las Aves de Corral , Vacunas Virales , Animales , Virus de la Enfermedad de Newcastle , Pollos , Filogenia , Convección , Irán , Vacunas Sintéticas/genética , Vacunación/veterinaria , Genotipo , Anticuerpos AntiviralesRESUMEN
Gastrointestinal symptoms in poultry are caused by several factors, such as infecting viruses. Several avian picornaviruses can cause diarrhea in these valuable animals. Poultry flocks in Iran suffer from gastrointestinal diseases, and information on picornaviruses is limited. In this study, two genera of avian picornaviruses were isolated from poultry and identified by the viral metagenomics. Fecal samples were collected from broiler chicken flocks affected with diarrhea from Gilan province Iran. The results showed that Eastern chicken flocks carried two genera of picornaviridae belonging to Sicinivirus A (SiV A) and Megrivirus C (MeV C). The Western chicken flocks carried SiV A based on whole-genome sequencing data. SiV A had type II IRES and MeV C contained a type IVB IRES 5'UTR. Phylogenetic results showed that all these three picornaviruses were similar to the Hungarian isolates. Interestingly, two different picornavirus genera were simultaneously co-infected with Eastern flocks. This phenomenon could increase and facilitate the recombination and evolution rate of picornaviruses and consequently cause this diversity of gastrointestinal diseases in poultry. This is the first report and complete genome sequencing of Sicinivirus and Megrivirus in Iran. Further studies are needed to evaluate the pathogenic potential of these picornaviruses.
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Picornaviridae , Enfermedades de las Aves de Corral , Animales , Pollos , Filogenia , Irán , Genoma Viral , Diarrea/veterinaria , Diarrea/genéticaRESUMEN
Marek's disease is a significant illness in chickens and a potential threat to the poultry industry worldwide. Marek's disease virus (MDV) causes immunosuppression and lymphoproliferative disease in chickens, but the turkey is an unusual host for the virus, and tumors caused by MDV in turkeys are unique. This study sampled 15 asymptomatic commercial turkey flocks (five spleens from each flock) at slaughter. Gallid alphaherpesvirus 2 (GaHV-2) was identified by PCR of spleen samples of two flocks. A phylogenetic analysis of the Meq gene was also performed. Sequencing and phylogenetic analysis revealed that the turkey GaHV-2 had genetic similarity with GaHV-2 strains recently detected in the Iranian commercial layer and breeder turkey flocks. This is the first time MDV has been detected in turkey flocks of Iran, and therefore, further assays including experimental inoculation to demonstrate pathotype characteristics in vivo are needed.
Nota de investigación- Virus de la enfermedad de Marek en parvadas comerciales de pavos en Irán. La enfermedad de Marek es una enfermedad importante de los pollos y una amenaza potencial para la industria avícola en todo el mundo. El virus de la enfermedad de Marek (MDV) causa inmunosupresión y una enfermedad linfoproliferativa en pollos, pero el pavo es un huésped inusual para este virus y los tumores causados por el virus de la enfermedad de Marek en pavos son únicos. En este estudio se recolectaron muestras de 15 lotes de pavos comerciales asintomáticos (cinco bazos de cada lote) en el momento del sacrificio. El Gallid alphaherpesvirus 2 (GaHV-2) fue identificado por PCR de muestras de bazo de dos parvadas. También se realizó un análisis filogenético del gene Meq. La secuenciación y el análisis filogenético revelaron que el GaHV-2 de pavo tenía una similitud genética con las cepas de GaHV-2 detectadas recientemente en parvadas de postura comerciales y de pavos reproductores iraníes. Esta es la primera vez que se detecta el virus de Marek en parvadas de pavos de Irán y, por lo tanto, se necesitan más ensayos, incluida la inoculación experimental, para demostrar las características del patotipo in vivo.
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Herpesvirus Gallináceo 2 , Enfermedad de Marek , Filogenia , Enfermedades de las Aves de Corral , Pavos , Animales , Enfermedad de Marek/virología , Enfermedad de Marek/epidemiología , Irán/epidemiología , Herpesvirus Gallináceo 2/genética , Herpesvirus Gallináceo 2/aislamiento & purificación , Herpesvirus Gallináceo 2/clasificación , Enfermedades de las Aves de Corral/virología , Enfermedades de las Aves de Corral/epidemiologíaRESUMEN
BACKGROUND: Infectious haematopoietic necrosis (IHN) is known as one of the most contagious systemic viral diseases in salmonids which can lead to significant mortality rates and negative impacts on the salmonid farming industry. Infectious haematopoietic necrosis virus (IHNV) was first detected in rainbow trout (Oncorhynchus mykiss) farms in Iran in 2003. OBJECTIVES: We conducted the present study to determine the detection of IHN genotypes in rainbow trout (O. mykiss) in farms in the central parts of Iran, using molecular and phylogenetic techniques. METHODS: Samples were collected from fries exhibiting clinical signs such as darkening of the skin, abdominal swelling, and loss of appetite. Phylogenetic analysis was performed by the neighbour-joining method, using MEGA 5.1 software. For phylogenetic analysis and genotyping of IHNV from central parts of Iran, the sequences of the glycoprotein gene were determined for two Iranian isolates (Jahad-UT1 and Jahad-UT2). RESULTS: Phylogenetic analysis revealed that the detected strains (Jahad-UT1 and Jahad-UT2 isolates) are closely related (97.23%-100%) to European isolates within genogroup 'E'. CONCLUSIONS: This finding indicates that Jahad-UT1 and Jahad-UT2 isolates have been widely transferred to Iran from European countries. Moreover, the nucleotide diversity of these Iranian isolates showed a close relationship with the North American and Asian isolates, although the Iranian isolates were collected from a smaller geographical area and within a shorter time period between 2014 and 2015.
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Enfermedades de los Peces , Virus de la Necrosis Hematopoyética Infecciosa , Oncorhynchus mykiss , Infecciones por Rhabdoviridae , Animales , Virus de la Necrosis Hematopoyética Infecciosa/genética , Irán/epidemiología , Filogenia , Genotipo , Enfermedades de los Peces/epidemiología , Infecciones por Rhabdoviridae/epidemiología , Infecciones por Rhabdoviridae/veterinaria , Glicoproteínas/genéticaRESUMEN
Marek's disease (MD) is a highly contagious, lymphoproliferative poultry disease caused by the oncogenic herpesvirus, serotype 1 Marek's disease virus (MDV-1), or Gallid herpesvirus 2 (GaHV-2). MDV strains have shown a continued evolution of virulence leading to immune failure, and MD cases continue to occur or surge. Meq, the major MDV-1 oncoprotein, induces T-cell neoplastic transformation through several mechanisms including inhibition of apoptosis, cell cycle regulation, and serum-anchorage independent growth. There is no current information on the MDV serotypes and pathotypes circulating in vaccinated commercial farms in Iran, where the birds are vaccinated at the hatchery with GaHV-2 and Meleagrid herpesvirus 1 (MeHV-1) vaccines. This study reports the molecular characterization of a GaHV-2 strain detected in 19 flocks of Iranian layer farms exhibiting MDV-1-like clinical signs and visceral lymphomas. Based on sequencing and phylogenetic analysis of the Meq gene, the Iranian GaHV-2 isolates could be divided into two separate clades regarding molecular features. The clade containing strains was closely related to Italian, Indian, and Hungarian virulent isolates, and the clade was related to American very virulent plus (vv+) isolates. For the first time, the MDV-1 virus was characterized by an outbreak in poultry flocks in Iran. Although MDV-1 strains obtained in Iran's present outbreak are presumably related to virulent (v) and vv+ pathotypes based on nucleotide, amino acid, and phylogenetic analysis of the viruses, they are not confirmed so far. Thus, it is highly recommended to perform further analyses to demonstrate the pathotype characteristics in vivo.
Caracterización molecular y análisis filogenético del virus de la enfermedad de Marek en Irán. La enfermedad de Marek (MD) es una enfermedad altamente contagiosa linfoproliferativa en la avicultura causada por el herpesvirus oncogénico, el virus de la enfermedad de Marek de serotipo 1 (MDV-1) o Gallid herpesvirus 2 (GaHV-2). Las cepas del virus de Marek han mostrado una evolución continua de virulencia que conduce a una falla inmunológica, y los casos de Marek continúan ocurriendo o aumentando. El gene Meq, codifica la principal oncoproteína de MDV-1, induce la transformación neoplásica de células T a través de varios mecanismos que incluyen la inhibición de la apoptosis, la regulación del ciclo celular y el crecimiento independiente del anclaje sérico. No hay información actual sobre los serotipos y patotipos del virus de Marek que circulan en las granjas comerciales vacunadas en Irán, donde las aves se vacunan en la planta de incubación con las vacunas GaHV-2 y Meleagrid herpesvirus 1 (MeHV-1). Este estudio reporta la caracterización molecular de una cepa del Gallid herpesvirus 2 detectada en 19 lotes de granjas de aves de postura iraníes que presentaron signos clínicos sugestivos del serotipo 1 del virus de la enfermedad de Marek y linfomas viscerales. Según la secuenciación y el análisis filogenético del gene Meq, los aislamientos iraníes de GaHV-2 podrían dividirse en dos clados separados con respecto a las características moleculares. El clado que contenía las cepas estaba estrechamente relacionado con los aislados virulentos de Italia, India y de Hungria y el clado estaba relacionado con los aislados americanos muy virulentos plus (vv+). Por primera vez, el serotipo 1 del virus de la enfermedad de Marek se caracterizó por un brote en parvadas avícolas en Irán. Aunque las cepas del virus de Marek, serotipo 1 obtenidas en el brote actual de Irán están presuntamente relacionadas con patotipos virulentos (v) y muy virulentos plus según el análisis de nucleótidos, aminoácidos y filogenético de los virus, hasta el momento no se han confirmado. Por lo tanto, se recomienda realizar más análisis para demostrar las características del patotipo in vivo.
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Herpesvirus Gallináceo 2 , Enfermedad de Marek , Enfermedades de las Aves de Corral , Aminoácidos , Animales , Pollos , Irán/epidemiología , Nucleótidos , Proteínas Oncogénicas/genética , Filogenia , Aves de CorralRESUMEN
OBJECTIVE: Himetobi P virus (HiPV) is an insect virus belonging to the genus Cripavirus in the Dicistroviridae family within the Picornavirales order. Himetobi P strain. Sh.Moghaddam is the first study reported, was isolated from the Laodelphax striatellus (small brown planthopper) of an internal chicken organ in Iran. DATA DESCRIPTION: Genomic analysis showed a nucleotide identity of 93.16% with the family Dicistroviridae, genus Triatovirus, and species Himetobi P. The genome assembly comprised 9227 bp, with a 38.8% GC content. Annotation of the genome showed 2 ORF, a total of 2 genes: including 2 coding sequences (CDs) (total) and 8 Miss features. Thus, the whole-genome sequence presented in this study serves as a platform for detecting new genes that may contribute to the pathogenicity of the Himetobi P strain. Sh.Moghaddam.
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Hemípteros , Virus ARN , Animales , Genómica , Hemípteros/genética , Secuenciación de Nucleótidos de Alto Rendimiento , Análisis de Secuencia de ADNRESUMEN
As neoplastic viruses have been affecting Iranian chicken farms more frequently in recent years, the first step in prevention may therefore be to genetically characterize and systematically identify their source and origin. Recently, we published a phylogenetic analysis based on the meq gene of Gallid alphaherpesvirus 2, commonly known as serotype 1 Marek's disease virus (MDV-1), that circulated in Iranian backyard and commercial chickens. In the current study, we are reporting for the first time the identification of a 298 aa meq protein containing only two PPPP motifs from an MDV-1-infected unvaccinated backyard turkey. This protein length has never been reported from any turkey species before. According to phylogenetic analysis, a close genetic relationship (0.68%) to several chicken-origin isolates such as the American vv + 648A strain was found. In addition, we identified a standard meq protein from a MDV-1-infected commercial chicken farm. In corroboration with our previous finding from other Iranian provinces, it is likely that the highly identical MDV-1 viruses currently circulating in Iranian chicken farms, which may be indicative of human role in the spread of the virus, have similar Eurasian origin. Our data suggest that regardless of the meq size, MDV-1 circulating in Iran are from different origins. On the other hand, meq sequences from bird species other than chicken have been reported but are very few. Our investigation suggests MDV-1 circulating in turkey do not have species-specific sequences.
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Herpesvirus Gallináceo 2 , Enfermedad de Marek , Enfermedades de las Aves de Corral , Animales , Pollos , Herpesvirus Gallináceo 2/genética , Humanos , Irán/epidemiología , Enfermedad de Marek/epidemiología , Enfermedad de Marek/prevención & control , Filogenia , Aves de Corral , Enfermedades de las Aves de Corral/epidemiologíaRESUMEN
Infectious bronchitis virus (IBV) and avian influenza virus (AIV) are two major respiratory infections in chickens. The coinfection of these viruses can cause significant financial losses and severe complications in the poultry industry across the world. To examine transcriptome profile changes during the early stages of infection, differential transcriptional profiles in tracheal tissue of three infected groups (i.e., IBV, AIV, and coinfected) were compared with the control group. Specific-pathogen-free chickens were challenged with Iranian variant-2-like IBV (IS/1494), UT-Barin isolates of H9N2 (A/chicken/Mashhad/UT-Barin/2017), and IBV-AIV coinfection; then, RNA was extracted from tracheal tissue. The Illumina RNA-sequencing (RNA-seq) technique was employed to investigate changes in the Transcriptome. Up- and downregulated differentially expressed genes (DEGs) were detected in the trachea transcriptome of all groups. The Kyoto Encyclopedia of Genes and Genomes pathway and Gene Ontology databases were examined to identify possible relationships between DEGs. In the experimental groups, upregulated genes were higher compared to downregulated genes. A more severe immune response was observed in the coinfected group; further, cytokine-cytokine receptor interaction, RIG-I-like receptor signaling, Toll-like receptor signaling, NOD-like receptor signaling, Janus kinase/signal transducer, and activator of transcription, and apoptotic pathways were important upregulated genes in this group. The findings of this paper may give a better understanding of transcriptome changes in the trachea during the early stages of infection with these viruses.
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Bronquitis , Coinfección , Infecciones por Coronavirus , Virus de la Bronquitis Infecciosa , Subtipo H9N2 del Virus de la Influenza A , Gripe Aviar , Enfermedades de las Aves de Corral , Animales , Bronquitis/genética , Bronquitis/veterinaria , Pollos , Perfilación de la Expresión Génica , Virus de la Bronquitis Infecciosa/genética , Subtipo H9N2 del Virus de la Influenza A/genética , Gripe Aviar/genética , Irán , Enfermedades de las Aves de Corral/genética , ARN , Tráquea , Transcriptoma/genéticaRESUMEN
Infectious bronchitis virus (IBV) is one of the major threats to the poultry industry, with significant economic consequences. Despite strict measures, the disease is difficult to control worldwide. Experimental evidence demonstrates that the severity of IBV is affected by the genetic background of the chicken, and the selection of appropriate breeds can increase production efficiency. Therefore, the aim of the present study was to assess the strength of the immune response to IBV in tracheal tissues of Ross 308 and Cobb 500 broiler chickens by evaluating transcriptome changes, focusing on immune responses and the viral load in tracheal tissues two days after IBV infection. We identified 899 and 1350 differentially expressed genes (DEGs) in the Cobb 500 and Ross 308 experimental groups compared to their respective control groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated the involvement of signaling pathways (Toll-like receptor [TLR], NOD-like receptor [NLR], and RIG-I-like receptor [RLR] signaling pathways). Interestingly, the RLR signaling pathway appears to be affected only in the Cobb hybrid. Furthermore, the viral loads in tracheal samples obtained from the Ross challenged group were significantly higher than those of the Cobb challenged group. The results of this study indicated that the host transcriptional response to IBV infection as well as the viral load can differ by hybrid. Furthermore, genes such as TLR-3, ChIFN-α, MDA5, LGP2, IRF-7, NF-κB, and TRIM25 may interfere with IBV proliferation.
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Infecciones por Coronavirus , Virus de la Bronquitis Infecciosa , Enfermedades de las Aves de Corral , Animales , Pollos , Infecciones por Coronavirus/genética , Infecciones por Coronavirus/veterinaria , Virus de la Bronquitis Infecciosa/genética , Transcriptoma , Carga ViralRESUMEN
Wild aquatic birds are the main natural host reservoir of avian influenza viruses (AIV). Migratory aquatic birds can translocate AI viruses over wide geographic distances. AIV may be transmitted reciprocally at the wild bird-poultry interface, increasing viral variability and potentially driving the zoonotic potential of these viruses. A cross-sectional study on AIV and several further avian viral pathogens conducted in 396 trapped migratory aquatic birds traded at live bird markets (LBM) in northern Iran identified 11 AIV-positive cases. The 10 identified H9N2 viral sequences fell into wild bird H9 lineage Y439; in addition, an H10N3 virus of Eurasian lineage was detected. Ten samples contained low viral loads of avian coronavirus but could not be further characterized. Although traditional trading of live-trapped wild birds provides income for hunters, particularly during fall migration periods, it increases the risk of introducing new AIV strains from the natural reservoir to poultry kept at LBMs and, potentially, to traders and customers. Banning these birds from poultry trading lines would lower such risks considerably.
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BACKGROUND AND OBJECTIVES: Avian respiratory disease complex (RDC) is one of the most detrimental economic diseases that affected different parts of the world. Various pathogens cause the disease, but the most significant viral pathogens include avian influenza virus (AIV), infectious bronchitis virus (IBV), and Newcastle disease virus (NDV) are the most prevalent. To detect these pathogens, various methods have been discovered in the last decades. Detection and characterization of viruses by metagenomics methods have improved our knowledge about the role of virome in the avian complex respiratory disease. MATERIALS AND METHODS: This research investigates the viral pathogen populations that mostly participate in emerging these diseases using the NGS method RNA-sequencing. In surveillance of ten broiler farms from different cities with respiratory symptoms, trachea samples were collected to determine the pathogenic virome causing the disease. RESULTS: In this metagenomics analysis, nine viral families were identified, comprising 72.82% of RNA viruses, 24.32% of RT viruses, and 2.86% of DNA viruses. RNA viruses had the highest contribution to the respiratory disease complex instead of disease, including paramyxoviridae, orthomyxoviridae, coronaviridae, and picornaviridae viruses. Other viruses from the RNA viruses and DNA virus families were also identified in addition to these results. CONCLUSION: This research suggests that studies of pathogenic viromes in different diseases can help monitor different diseases and predict their future occurrence.
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Following recent Newcastle disease virus (NDV) outbreaks in Iranian poultry farms which were mostly associated with lesions of the avian gastrointestinal tract, it was speculated that the scale of the outbreaks could be attributed in part to co-circulating infectious agents or a new NDV genotype/subgenotype. This speculation was due to the isolation of a few 5th panzootic subgenotype VII.2 viruses from Iranian poultry farms in 2017. Samples from different species of commercial and domestic birds were collected from different provinces of Iran, 19 of which were selected for the current study. Phylogenetic analyses showed that the recent outbreaks have been caused by only one agent, i.e. the distinctive NDV subgenotype VII.1.1 (previously known VIIl) viruses that seem to be circulating predominantly in Iran, but have also been sporadically reported from Iraq among neighbouring countries. At most, 96.3-96.7% BLAST identity to non-Iranian VII.1.1 isolates was observed. Genetic distance values of <1% were indicative of high similarity between the isolates, but the values were approaximately 2% when the current isolates were compared to the earliest recorded Iranian VII.1.1 viruses isolated in 2010. Using Bayesian analysis, annual mutation rates of 1.7156E-3 (strict) and 1.9902E-3 (relaxed) over 11 years were obtained. In addition, we report that our laboratories have not detected any genotype XIII strains since 2011. Following up on previous reports, we concluded that currently, and except in Columbiforms, subgenotype VII.1.1 may likely be the predominant subgenotype in many bird species in Iran despite the subgenotype VII.2 being predominant in neighbouring countries.
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Enfermedad de Newcastle , Enfermedades de las Aves de Corral , Animales , Teorema de Bayes , Pollos , Genotipo , Irán/epidemiología , Enfermedad de Newcastle/epidemiología , Virus de la Enfermedad de Newcastle/genética , FilogeniaRESUMEN
The present study aimed to evaluate the effects of inactivated vaccines combining Mass and Dutch variants as vaccine boosters after H120 priming on inhibiting variant 2 viral load in the kidneys (as the target organ) and reducing fecal shedding. Ciliostasis score and antibody response were investigated as well. A total of 150 specific-pathogen-free (SPF) chicken were divided into six groups. All groups were vaccinated with a single dose of attenuated H120 vaccine except for two (no vaccine groups). Then, three groups received booster vaccines with inactivated polyvalent vaccines. At the 42 day of age, all groups were challenged with variant 2 viruses except for one (no vaccine group). Next, antibody response and infectious bronchitis virus viral load in kidneys and fecal shedding were evaluated by the enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction. Then the ciliostasis score was investigated. In general, a vaccination program including a mass serotype attenuated vaccine (H120) as priming and polyvalent vaccines can significantly protect chickens against variant 2 infection through reducing viral load in kidneys and fecal shedding. Furthermore, the vaccination program can decrease ciliostasis in the epithelial ciliary tissue.
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Infecciones por Coronavirus , Virus de la Bronquitis Infecciosa , Enfermedades de las Aves de Corral , Vacunas Virales , Animales , Anticuerpos Antivirales , Pollos , Infecciones por Coronavirus/prevención & control , Infecciones por Coronavirus/veterinaria , Enfermedades de las Aves de Corral/prevención & control , Vacunación , Vacunas de Productos InactivadosRESUMEN
BACKGROUND AND OBJECTIVES: Infection with Infectious bronchitis virus (IBV) and avian pathogenic Escherichia coli (APEC) is an important respiratory infection worldwide. Apoptosis is a physiological process of cell death that occurs as part of normal development and responds to a variety of physiological and pathophysiological stimuli. The identification of molecular mechanisms of action or inaction of key apoptotic proteins is important. This study aimed to investigate apoptotic related genes in the trachea tissue of infected (IBV variant 2, and APEC serotype O78: K80) SPF chickens group compared to the control group. MATERIALS AND METHODS: Forty SPF chickens was divided into 2 groups. Differential transcriptional profile in the infected SPF chickens trachea tissue was compared to those of control group in the early stage of infection by Illumina RNA-seq technique paired-end and strand-specific sequencing. Differentially expressed genes (DEGs) of transcriptome profiling of the trachea from the infected group were identified. Gene ontology category, KEGG pathway, and STRING analysis were analyzed to identify relationships among differentially expressed genes. RESULTS: Twenty-eight apoptotic genes were identified. They consisted of six pathways related to cell death: the extrinsic pathway, intrinsic pathway, endoplasmic reticulum stress pathway, MAPK signaling pathway, and cell death by NFkB and activates mTOR pathway and some regulator and apoptosis inhibitors. CONCLUSION: All of the apoptotic genes in our study were up-regulated. Among these genes, the more fold change value was for TRADD and BCL2A1 genes, and the less fold change value was for MAP3K14, NFKB1, PIK3CB, and ITPR2 genes.
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Avian infectious bronchitis virus is one of the most important gammacoronaviruses, which causes a highly contagious disease. In this study, we investigated changes in the proteome of kidney tissue of specific-pathogen-free (SPF) chickens that were infected with an isolate of the nephrotropic variant 2 genotype (IS/1494/06) of avian coronavirus. Twenty 1-day-old SPF White Leghorn chickens were randomly divided into two groups, each comprising 10 chickens, which were kept in separate positive-pressure isolators. Chickens in group A served as a virus-free control group up to the end of the experiment, whereas chickens in group B were inoculated with 0.1 ml of 104.5 EID50 of the IBV/chicken/Iran/UTIVO-C/2014 isolate of IBV, and kidney tissue samples were collected at 2 and 7 days post-inoculation (dpi) from both groups. Sequencing of five protein spots at 2 dpi and 22 spots at 7 dpi that showed differential expression by two-dimensional electrophoresis (2DE) along with fold change greater than 2 was done by MS-MALDI/TOF/TOF. Furthermore, the corresponding protein-protein interaction (PPI) networks at 2 and 7 dpi were identified to develop a detailed understanding of the mechanism of molecular pathogenesis. Topological graph analysis of this undirected PPI network revealed the effect of 10 genes in the 2 dpi PPI network and nine genes in the 7 dpi PPI network during virus pathogenesis. Proteins that were found by 2DE analysis and MS/TOF-TOF mass spectrometry to be down- or upregulated were subjected to PPI network analysis to identify interactions with other cellular components. The results show that cellular metabolism was altered due to viral infection. Additionally, multifunctional heat shock proteins with a significant role in host cell survival may be employed circuitously by the virus to reach its target. The data from this study suggest that the process of pathogenesis that occurs during avian coronavirus infection involves the regulation of vital cellular processes and the gradual disruption of critical cellular functions.