Evaluating drained water quality in a pilot worm-sludge treatment reed bed planted with Arundo donnas in the Mediterranean climate.

This study evaluated the impact of incorporating earthworms (Eisenia fetida) on the drained water quality from a sludge treatment reed bed. The experiment encompassed four setups of treatment beds in two replicates: planted with Arundo donax and addition of earthworms, planted without earthworms, unplanted with earthworms, and treatment bed without plants nor earthworms as control. The units were fed every two weeks with mixed sewage sludge, a blend of primary and secondary sludge over 24 cycles. The mixed sewage sludge had mean dry and volatile solid contents of 24.71 g.DS.L-1 (± 13.67) and 19.14 g.VS.L-1 (± 10.29) resulting a sludge loading rate of 43.59 kg.DS.m-2.year-1 (± 14.49). The inclusion of earthworms in the planted unit reduced release masses of total suspended solids, chemical oxygen demand, nitrate and phosphorous by 43, 45, 75 and 45 % compared to the planted unit. Plant biomass production increased by 43 % with the earthworm presence. The removal efficiency of the units improved after a ramp-up phase (after six months feeding) of which the concentration of TSS, COD and Escherichia coli met limits for water reuse while nitrogen components and phosphorous surpassed the limits. The planted unit with earthworms removed 99 and 99 % of TSS and COD, respectively. Overall, water loss namely through evapotranspiration and earthworm hydration need, positively correlated with pollutant concentration, and earthworm-planted unit had 46 % higher water loss compared to control unit.

Water balance analysis in a novel pilot-scale of the Worm-sludge treatment reed bed (W-STRB) planted with Arundo donax.

A one-year study of sludge treatment reed bed assisted with earthworms (W-STRB) was conducted in a temperate climate. The effects of using Eisenia fetid and Arundo donax on W-STRB water balance (WB) and dewatering efficiency (DE) were investigated. Four different bed configurations were tested: worm-planted (WP), planted (P), worm-control (W), and control (C), duplicated resulting in a total of eight units. The beds received a total of 24 cycles of mixed sewage sludge twice per month (average loading rate: 43.59 kg.DS. m-2.year-1). It was found seasonal variation played a significant role in WB and DE. During the dry season, the thickness of the residual sludge (RS) layer was less than 1 cm, with a dry solid (DS) content of over 80%, in contrast, the wet season indicated an increase in RS thickness to nearly 30 cm (DS < 15 % for all units). The WP unit exhibited the lowest RS accumulation, 22% less than the P, W, and C units. The subsurface layer had a 5% lower volatile solids (VS) content compared to the surface layers. After 132 days of a final resting, WP unit had the highest RS volume reduction of 65 % (DS = 71 % and VS = 53 %) and a RS thickness of 6 cm indicating a 10 % higher stabilization compared to P unit. The population of earthworms was 30% higher in the WP unit compared to the W unit. As the subsurface DS exceeded 20 % during the dry season, the population increased. The WP unit showed a 43% higher above-ground plant biomass compared to the P unit. In WB analysis, evapotranspiration (ET) was 46% higher in the WP unit (average daily ET = 5.44 mm in the dry season). The main process of water loss was through drainage and Awhile water content in RS layer was 57 % during feeding period. The water percolation rate of all units decreased by 99%, particularly during the wet season, reaching less than 0.1 m.day-1.

Sludge Treatment Reed Bed under different climates: A review using meta-analysis.

Sludge Treatment Reed Beds (STRBs) have been used worldwide over the past few decades. This review aims to overarchingly identify and appraise the currently available knowledge of STRB technology and discern climatic patterns through Meta-Analysis (MA). We systematically searched Google Scholar, Scopus, and Web of Science databases (up to Dec 2021) via a combination of keywords to identify English-language studies published in peer-reviewed journals. Of 142 potential articles, 73 studies met the present review objectives and inclusion criteria. Four STRB classifications including typical STRB, earthworm STRB, Sludge Treatment Electro Wetland (STEW), and earthworm STEW were found since 1990. The data and information on STRBs' configuration, operational parameters in terms of location, type of sewage sludge, study scale, Sludge Loading Rate (SLR), Dry Solid (DS), the proportion of Volatile Solid to DS (VS/DS), and their association with the feeding and resting modes were extracted from the selected articles. The analysis was focused on the interconnections between operational parameters and system efficiency for Temperate type 1 (low intensity of solar radiation), Temperate type 2 (high intensity of solar radiation), and Tropical climates. Based on MA, we found the average SLRs of 50, 70, and 101 Kg.DM.m-2.year-1 for Temperate type 1, Temperate type 2, and Tropical climates respectively, and DS during the feeding of 33 %, 35 %, and 40 %. A qualitative comparison of Arid and Polar climates was also performed given the reduced number of studies available in these climates. The volume of the sludge reduced was 60 % higher and the height of accumulated sludge was annually 2 cm in the earthworm STRBs, and STEWs compared to typical STRBs, which was 6 cm annually in Tropical climates. Correlation analysis, media characterization, list of plant species, and the removal efficiency of STRBs in the residual sludge and leachate are mentioned as well.

Targeting the metabolism of cancer stem cells by energy disruptor molecules.

Cancer stem cells (CSCs) have been identified in various tumor types. CSCs are believed to contribute to tumor metastasis and resistance to conventional therapy. So targeting these cells could be an effective strategy to eliminate tumors and a promising new type of cancer treatment. Alterations in metabolism play an essential role in CSC biology and their resistance to treatment. The metabolic properties pathways in CSCs are different from normal cells, and to some extent, are different from regular tumor cells. Interestingly, CSCs can use other nutrients for their metabolism and growth. The different metabolism causes increased sensitivity of CSCs to agents that disrupt cellular homeostasis. Compounds that interfere with the central metabolic pathways are known as energy disruptors and can reduce CSC survival. This review highlights the differences between regular cancer cells and CSC metabolism and discusses the action mechanisms of energy disruptors at the cellular and molecular levels.

Apoptosis Effects of Oxalis corniculata L. Extract on Human MCF-7 Breast Cancer Cell Line:.

Background: Recently, the non-toxic properties of natural plant products have gained more focus as anticancer agents. Therefore, this study aimed to assess the apoptosis effects of the ethanolic extract of Oxalis corniculata on the MCF-7 breast cancer cell line.Materials and Methods: In this experimental study, aerial parts of O. corniculata were collected in Lahijan city (Iran), and after confirmation, they were dried and extracted with ethanol for 24 h. Then, the total phenolic and flavonoid contents of the extract were measured. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay was used to measure the antioxidant properties of the extract. Selected cell lines (MCF-7 and human dermal fibroblast) were cultured in 6-wells dishes (1×106 cells/well). After 72 h of treating the extract, cytotoxicity was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The expression of apoptotic genes (such as p53, bcl-2, bax, and CD95) was studied by real-time polymerase chain reaction (PCR). Results: The extract's total phenolic content was 31.30±02 µg of gallic acid equivalents/mg of dry extract, and the total flavonoid content was 49.61±04 µg of quercetin as equivalents/mg of extract. The antioxidant activity ofO. corniculata was measured at the dose of 619.2 µg/µl, indicating that it decreases cancer cell viability and enhances apoptosis. Within the half maximal inhibitory concentrations, real-time PCR revealed substantial increases in p53 (P<0.001), CD95 (P<0.05), and bcl-2 expression (P<0.05) in MCF-7 cells treated with O. corniculata. Conclusion: This study suggests that O. corniculata may cause apoptosis by oxidative stress in cancer cells.[GMJ.2022;11:e2484].

Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound Regeneration.

Loss of skin integrity can lead to serious problems and even death. In this study, for the first time, the effect of exopolysaccharide (EPS) produced by cold-adapted yeast R. mucilaginosa sp. GUMS16 on a full-thickness wound in rats was evaluated. The GUMS16 strain's EPS was precipitated by adding cold ethanol and then lyophilized. Afterward, the EPS with polycaprolactone (PCL) and gelatin was fabricated into nanofibers with two single-needle and double-needle procedures. The rats' full-thickness wounds were treated with nanofibers and Hematoxylin and eosin (H&E) and Masson's Trichrome staining was done for studying the wound healing in rats. Obtained results from SEM, DLS, FTIR, and TGA showed that EPS has a carbohydrate chemical structure with an average diameter of 40 nm. Cell viability assessments showed that the 2% EPS loaded sample exhibits the highest cell activity. Moreover, in vivo implantation of nanofiber webs on the full-thickness wound on rat models displayed a faster healing rate when EPS was loaded into a nanofiber. These results suggest that the produced EPS can be used for skin tissue engineering applications.

A novel pilot and full-scale constructed wetland study for glass industry wastewater treatment.

Industrial wastewaters represent a serious threat to the environment due to their variable and complex composition. Though mostly mechanical systems are used for treatment of such wastewater, there is growing need for sustainable and cost-effective solutions, especially in low-income regions. In this study, a horizontal sub-surface flow Constructed Wetland (HSFCW) system was used for the first time to treat wastewater from a glass manufacturing industry in Iran. In order to de-risk the treatment approach, a pilot system consisting of a settling tank and a HSFCW was first tested for 4 months. The results of the pilot study were then used to build the full-scale CW system treating 10 m³/day. In general, the tested design proved to be very effective reaching high removal rates of BOD5, COD, and TSS (90, 90, and 99, respectively), as also for TN and TP (>90%). The high efficiency of the tested system allowed for the recycle and reuse of the treated effluent in the glass manufacturing processes, reducing this way the fresh water consumption in the glass industry and the related operational costs.

Production, characterization and biological activities of exopolysaccharides from a new cold-adapted yeast: Rhodotorula mucilaginosa sp. GUMS16.

Lately, it has been proved that yeast exopolysaccharides (EPS) are potentially applicable biopolymers, a fact that has led to incremental needs for their assessment. The current study is based on the biochemical and molecular level identification of the novel cold-adapted yeast Rhodotorula mucilaginosa sp. GUMS16. Possible antioxidant and antiproliferative activities, as well as extraction and characterization of the GUMS16-produced EPS, were assessed during the course of this study. The results indicated that the strain of GUMS16 is a cold-adapted yeast with growth capability at 4 °C and an approximate EPS production yield of 28.5 g/L which are characterized as highly branched beta-D-glucan having glucose and mannose residues (85:15 mol%, respectively) with an average molecular weight of 84 kDa. In comparison to hyaluronic acid, DPPH, and OH, the scavenging activity attributed to the GUMS16-produced EPS was higher alongside being dose-dependent. The biocompatibility profile of the EPS was well-recognized based on its zero-cytotoxicity rate on a normal cell model. Collectively, the favorable properties of the EPS accentuate their potential as biocompatible compound suitable for subsequent pharmaceutical and industrial applications.