RESUMEN
The genome sequences of enterohaemorrhagic E. coli O157:H7 strains show multiple open-reading frames with collagen-like sequences that are absent from the common laboratory strain K-12. These putative collagens are included in prophages embedded in O157:H7 genomes. These prophages carry numerous genes related to strain virulence and have been shown to be inducible and capable of disseminating virulence factors by horizontal gene transfer. We have cloned two collagen-like proteins from E. coli O157:H7 into a laboratory strain and analysed the structure and conformation of the recombinant proteins and several of their constituting domains by a variety of spectroscopic, biophysical, and electron microscopy techniques. We show that these molecules exhibit many of the characteristics of vertebrate collagens, including trimer formation and the presence of a collagen triple helical domain. They also contain a C-terminal trimerization domain, and a trimeric α-helical coiled-coil domain with an unusual amino acid sequence almost completely lacking leucine, valine or isoleucine residues. Intriguingly, these molecules show high thermal stability, with the collagen domain being more stable than those of vertebrate fibrillar collagens, which are much longer and post-translationally modified. Under the electron microscope, collagen-like proteins from E. coli O157:H7 show a dumbbell shape, with two globular domains joined by a hinged stalk. This morphology is consistent with their likely role as trimeric phage side-tail proteins that participate in the attachment of phage particles to E. coli target cells, either directly or through assembly with other phage tail proteins. Thus, collagen-like proteins in enterohaemorrhagic E. coli genomes may have a direct role in the dissemination of virulence-related genes through infection of harmless strains by induced bacteriophages.
Asunto(s)
Colágeno/metabolismo , Escherichia coli O157/metabolismo , Profagos/genética , Conformación Proteica , Proteínas Recombinantes/química , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , Colágeno/genética , Escherichia coli O157/genética , Escherichia coli O157/patogenicidad , Microscopía Electrónica de Rastreo , Datos de Secuencia Molecular , Sistemas de Lectura Abierta/genética , Estructura Terciaria de Proteína , Proteínas Recombinantes/ultraestructura , Análisis de Secuencia de ADN , Toxinas Shiga/genética , Especificidad de la Especie , Ultracentrifugación , VirulenciaAsunto(s)
Cromosomas Humanos X/genética , Empalme Alternativo , Secuencia de Aminoácidos , Secuencia de Bases , Mapeo Cromosómico , Biología Computacional , ADN Complementario/genética , Bases de Datos Genéticas , Bases de Datos de Proteínas , Exones , Humanos , Datos de Secuencia Molecular , Sistemas de Lectura Abierta/fisiología , Alineación de SecuenciaRESUMEN
The rapid pace at which genomic and proteomic data is being generated necessitates the development of tools and resources for managing data that allow integration of information from disparate sources. The Human Protein Reference Database (http://www.hprd.org) is a web-based resource based on open source technologies for protein information about several aspects of human proteins including protein-protein interactions, post-translational modifications, enzyme-substrate relationships and disease associations. This information was derived manually by a critical reading of the published literature by expert biologists and through bioinformatics analyses of the protein sequence. This database will assist in biomedical discoveries by serving as a resource of genomic and proteomic information and providing an integrated view of sequence, structure, function and protein networks in health and disease.
Asunto(s)
Bases de Datos de Proteínas , Proteínas/metabolismo , Proteómica , Biología Computacional , Enfermedad , Genómica , Humanos , Almacenamiento y Recuperación de la Información , Internet , Unión Proteica , Procesamiento Proteico-Postraduccional , Proteínas/química , Proteínas/genética , Proteoma/química , Proteoma/genética , Proteoma/metabolismo , Especificidad por Sustrato , Vocabulario ControladoRESUMEN
Human Protein Reference Database (HPRD) is an object database that integrates a wealth of information relevant to the function of human proteins in health and disease. Data pertaining to thousands of protein-protein interactions, posttranslational modifications, enzyme/substrate relationships, disease associations, tissue expression, and subcellular localization were extracted from the literature for a nonredundant set of 2750 human proteins. Almost all the information was obtained manually by biologists who read and interpreted >300,000 published articles during the annotation process. This database, which has an intuitive query interface allowing easy access to all the features of proteins, was built by using open source technologies and will be freely available at http://www.hprd.org to the academic community. This unified bioinformatics platform will be useful in cataloging and mining the large number of proteomic interactions and alterations that will be discovered in the postgenomic era.
