[The implementation of computer model in research of dynamics of proliferation of cells of thyroid gland follicle].

The article deals with the results of computational experiments in research of dynamics of proliferation of cells of thyroid gland follicle in normal condition and in the case of malignant neoplasm. The model studies demonstrated that the chronic increase of parameter of proliferation of cells of thyroid gland follicle results in abnormal behavior of numbers of cell cenosis of thyroid gland follicle. The stationary state interrupts, the auto-oscillations occur with transition to irregular oscillations with unpredictable cell proliferation and further to the "black hole" effect. It is demonstrated that the present medical biologic experimental data and theory propositions concerning the structural functional organization of thyroid gland on cell level permit to develop mathematical models for quantitative analysis of numbers of cell cenosis of thyroid gland follicle in normal conditions. The technique of modeling of regulative mechanisms of living systems and equations of cell cenosis regulations was used

[Computer simulation of thyroid regulatory mechanisms in health and malignancy].

The paper describes a computer model for regulation of the number of thyroid follicular cells in health and malignancy. The authors'computer program for mathematical simulation of the regulatory mechanisms of a thyroid follicular cellular community cannot be now referred to as good commercial products. For commercialization of this product, it is necessary to draw up a direct relation of the introduced corrected values from the actually existing normal values, such as the peripheral blood concentrations of thyroid hormones or the mean values of endocrine tissue mitotic activity. However, the described computer program has been also used in researches by our scientific group in the study of thyroid cancer. The available biological experimental data and theoretical provisions on thyroid structural and functional organization at the cellular level allow one to construct mathematical models for quantitative analysis of the regulation of the size of a cellular community of a thyroid follicle in health and abnormalities, by using the method for simulation of the regulatory mechanisms of living systems and the equations of cellular community regulatory communities.

[Procedure for determination of individual sensitivity to antitumor drugs].

The present paper proposes to employ the cultured tumor cells of the breast and chick fibroblasts after long-term cultivation (for above 24 days) to determine their individual drug sensitivity and, as a criterion of cell damage, to use the percent of destruction of the cell layer formed in the wells 24 hours after drug insertion. It also presents the comparative results of tests of 2 cellular models that have been used to determine the in vitro sensitivity of the cells of breast cancer and chick fibroblasts to melfalan and its complex compound with copper acetylacetonate - MOK*M. At the same time, the cytotoxic activity of MOK*M and melfalan against tumor cells has been not shown to differ greatly (16.02+/-1.85 and 15.71+/-0.65% cell layer destruction, respectively), but the same activity of MOK*M against the model of intact cells (chick fibroblasts) was much less (15.23+/-1.97%) than that of melfalan (95.39+/-1.11%). The test system proposed by the authors is of certain informative value and it may be used for the determination of the individual sensitivity of tumor cells to antitumor drugs.

[Differential trypan blue staining of tumor cells for the determination of apoptosis].

The present paper suggests that trypan blue should be used for differential staining of tumor cells in order to determine apoptosis. The proposed method makes it possible to simplify operations, to reduce the time required to carry out a test, and to enhance the precision of determining the course of apoptosis during routine investigations. The paper also gives data of the comparative studies of the content of apoptotic cells in experimental melanoma B-16 though differential trypan blue staining and classical morphostructural analysis. The difference in the values obtained by these two methods is shown to be 35%.

[Morphological changes in experimental melanoma B-16 effected by a conjugate of polyvinylpyrrolidone and metal-organic complex of copper and melphalan].

Data on morphological alterations in experimental melanoma B-16 induced by conjugate of polyvinylpyrrolidone and metal-organic complex of copper and melfalon are presented. The antitumor effect of the synthetic conjugate, which inhibits melanoma-16 growth, is adequately tolerated by bone marrow cells. Conjugate accumulation among the dividing cells inhibits the proliferatrive capability of malignant cells and causes other specific morphological changes to occur. It was found that polyvinylpyrrolidone conjugate can be used as a carrier whenever there is a problem of making certain drugs soluble and, thus, more marketable.

[Rat reproductive cells as biological indicators of the effect of environmental factors]. / Reproduktivnye kletki krys kak biologicheskie indikatory vliianiia faktorov okruzhaiushchei sredy.

The effect of one-month-long inhalation exposure of male rats to sulphureous anhydrate, phenol, ammonia or to the mixture of these substances with formaldehyde and lead acetate, on the parameters of spermatogenesis was studied. The exposure of animals to sulphureous anhydrate or to the mixture indicated above resulted in the asynchronization of spermatogenic cycle, which was more significant than that caused by the separate application of substances studied. This mixture induced a 20% reduction in the number of tubules, containing spermatogonia, and a 20-30% increase in the number of tubules, containing spermatozoa. The structural and functional study of spermatogenic epithelium, considering stages of spermatogenic cycle, indicated that some cell populations form associations that are targets for the toxic agents studied.

[Genetic effects of the new pesticide rovicurt]. / Geneticheskie éffekty novogo pestitsida rovikurta.

Administration of rovicoort, a new pesticide, in various single doses (75, 150, 380 mg/kg) to mongrel white male rats intragastrically on empty stomach showed that the doses of 75 and 150 mg/kg stimulated adaptive mechanisms of the organism resulting in the increase of diameter of both seminiferous tubules and the number of Sertoli cells as well as fertilizing capacity of spermatozoa. Dominant lethal mutation in early postmeiotic cells was induced after the drug exposure of 150 and 380 mg/kg doses.