Physio-Chemical and Biological Characterization of Novel HPC (Hydroxypropylcellulose):HAP (Hydroxyapatite):PLA (Poly Lactic Acid) Electrospun Nanofibers as Implantable Material for Bone Regenerative Application.

The research on extracellular matrix (ECM) is new and developing area that covers cell proliferation and differentiation and ensures improved cell viability for different biomedical applications. Extracellular matrix not only maintains biological functions but also exhibits properties such as tuned or natural material degradation within a given time period, active cell binding and cellular uptake for tissue engineering applications. The principal objective of this study is classified into two categories. The first phase is optimization of various electrospinning parameters with different concentrations of HAP-HPC/PLA(hydroxyapatite-hydroxypropylcellulose/poly lactic acid). The second phase is in vitro biological evaluation of the optimized mat using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay for bone regeneration applications. Conductivity and dielectric constant were optimized for the production of thin fiber and bead free nanofibrous mat. With this optimization, the mechanical strength of all compositions was found to be enhanced, of which the ratio of 70:30 hit a maximum of 9.53 MPa (megapascal). Cytotoxicity analysis was completed for all the compositions on MG63 cell lines for various durations and showed maximum cell viability on 70:30 composition for more than 48 hrs. Hence, this investigation concludes that the optimized nanofibrous mat can be deployed as an ideal material for bone regenerative applications. In vivo study confirms the HAP-HPC-PLA sample shows more cells and bone formation at 8 weeks than 4 weeks.

Optimisation of microwave-assisted extraction (MAE) of anthraquinone and flavonoids from Senna alata (L.) Roxb.

This paper investigates the optimum processing conditions of microwave assisted extraction (MAE) of anthraquinone (aloe emodin, AE) and flavonoids (kaempferol 3-gentiobioside, K3G and kaempferol, KA) from Senna alata (L.) Roxb. The kinetic study indicates that MAE showed a greater extraction rate, compared to ultrasonic-assisted and maceration, due to the enhanced power which altered the leaf microstructures. The optimisation was undertaken using one-factor-at-a-time, two-level factorial design and central composite design were used to maximise the yield of the target compounds. The optimum yield of K3G (4.27 mg/g DW), KA (8.54 mg/g DW) and AE (0.86 mg/g DW) was obtained at 90.5% ethanol, microwave power of 18.6 W/mL with a desirability of 0.82. In addition, the yield of K3G and KA is correlated positively with the antioxidant activity.

Biodiesel synthesized from waste cooking oil in a continuous microwave assisted reactor reduced PM and NOx emissions.

A synergistic effect of the activated limestone-based catalyst (LBC) and microwave irradiation on the transesterification of waste cooking oil (WCO) was screened using a two-level factorial design and response surface methodology. The catalyst was prepared using a wet-impregnation method and was characterised for its surface element, surface morphology, surface area and porosity. The reaction was performed in a purpose-built continuous microwave assisted reactor (CMAR), while the conversion and yield of biodiesel were measured using a gas chromatography. The results showed that the catalyst loading, methanol to oil molar ratio and the reaction time significantly affect the WCO conversion. The optimum conversion of oil to biodiesel up to 96.65% was achieved at catalyst loading of 5.47 wt%, methanol to oil molar ratio of 12.21:1 and the reaction time of 55.26 min. The application of CMAR in this work reduced the transesterification time by about 77% compared to the reaction time needed for a conventional reactor. The biodiesel produced in this work met the specification of American Society for Testing and Materials (ASTM D6751). Engine test results shows the biodiesel has a lower NOx and particulate matters emissions compared to petrodiesel.

Effect of Solution Properties and Operating Parameters on Needleless Electrospinning of Poly(Ethylene Oxide) Nanofibers Loaded with Bovine Serum Albumin.

BACKGROUND: This paper presents the effect of solution properties and operating parameters of polyethylene oxide (PEO) based nanofiber using a wire electrode-based needleless electrospinning. METHODS: The feed solution was prepared using a PEO dissolved in water or a water-ethanol mixture. The PEO solution is blended with Bovine Serum Albumin protein (BSA) as a model drug to study the effect of the electrospinning process on the stability of the loaded protein. The polymer solution properties such as viscosity, surface tension, and conductivity were controlled by adjusting the solvent and salt content. The morphology and fiber size distribution of the nanofiber was analyzed using scanning electron microscopy. RESULTS: The results show that the issue of a beaded nanofiber can be eliminated either by increasing the solution viscosity or by the addition of salt and ethanol to the PEO-water system. The addition of salt and solvent produced a high frequency of smaller fiber diameter ranging from 100 to 150 nm. The encapsulation of BSA in PEO nanofiber was characterized by three different spectroscopy techniques (i.e. circular dichroism, Fourier transform infrared, and fluorescence) and the results showed the BSA is well encapsulated in the PEO matrix with no changes in the protein structure. CONCLUSION: This work may serve as a useful guide for a drug delivery industry to process a nanofiber at a large and continuous scale with a blend of drugs in nanofiber using a wire electrode electrospinning.

An Overview of Chitosan Nanofibers and their Applications in the Drug Delivery Process.

Chitosan is a polycationic natural polymer which is abundant in nature. Chitosan has gained much attention as natural polymer in the biomedical field. The up to date drug delivery as well as the nanotechnology in controlled release of drugs from chitosan nanofibers are focused in this review. Electrospinning is one of the most established and widely used techniques for preparing nanofibers. This method is versatile and efficient for the production of continuous nanofibers. The chitosan-based nanofibers are emerging materials in the arena of biomaterials. Recent studies revealed that various drugs such as antibiotics, chemotherapeutic agents, proteins and anti-inflammatory analgesic drugs were successfully loaded onto electrospun nanofibers. Chitosan nanofibers have several outstanding properties for different significant pharmaceutical applications such as wound dressing, tissue engineering, enzyme immobilization, and drug delivery systems. This review highlights different issues of chitosan nanofibers in drug delivery applications, starting from the preparation of chitosan nanofibers, followed by giving an idea about the biocompatibility and degradation of chitosan nanofibers, then describing how to load the drug into the nanofibers. Finally, the major applications of chitosan nanofibers in drug delivery systems.