Host-Parasitoid Relationship between Apis mellifera (Linnaeus, 1758) and Senotainia tricuspis (Meigen, 1838) (Diptera, Sarcophagidae): Fly Aggression Behavior and Infestation Rates of Senotainiosis.

Senotainia tricuspis (Meigen, 1838) is a Sarcophagid dipteran endoparasitoid of Apis mellifera L., and myiasis, caused by this fly, is reported in several European, North African and Middle Eastern countries. Nevertheless, very little knowledge concerning the aggression and parasitisation behavior of S. tricuspis toward A. mellifera is available in the scientific literature, and the temporal pattern of aggression remains unclear. The aim of this investigation was to describe the aggressive behavior of S. tricuspis and to provide data on pupation and adult emergence in order to identify further tools for the control of senotainiosis in beekeeping. Data were collected in an apiary in Pisa province (Tuscany, Italy), where observations of aggressive behavior were conducted indirectly by using a VHS camera and also directly by an observer. Four behavioral categories of the attack were described. A total of 55 aggressions, 21 beecatchers, 104 chases and 6 parasitization events were recorded with the camera. Slow-motion recording analyses of the parasitization episodes resulted in contact of at least 1/6 s between the parasitoid and the host. Through four days of direct observations, a total of 1633 aggression events were recorded. The daily temporal pattern of the number of aggressions showed two main peaks: one during the morning hours (i.e., from 10:00 to 11:00) and one in the afternoon (i.e., from 15:00 to 17:00). The morphometric data on the first-instars of S. tricuspis allowed us to hypothesize a penetration in the bee through its prothoracic spiracle as a modality of entrance in the host body. Third-instars successfully pupate when sinking in topsoil or clay soil, and adults emerge when left to a 4 °C overwintering period of six months. Furthermore, the high mortality rate of those larvae that did not sink and did not pupate successfully suggests that reaching a certain depth in the soil is a determining factor for larvae survival and that mulch and/or minimum soil tillage could prevent severe senotainiosis in apiaries.

Evaluation of a smartphone-based methodology that integrates long-term tracking of mobility, place experiences, heart rate variability, and subjective well-being.

This study presents MyGävle, a smartphone application that merge long-term tracking of mobility data, heart rate variability and subjective and objective well-being records. Developed to address the challenges faced in researching healthy and sustainable lifestyles, this app serves as a pioneering implementation of Real-life Long-term Methodology (ReaLM). After eight months' use by 257 participants from Gävle (Sweden), we evaluate the completeness, accuracy, validity, and consistency of all data collected. MyGävle produced remarkable results as a ReaLM method. On average, it precisely tracked participants daily locations for approximately 8 h and accurately collected heart-rate variability values throughout the day (12 h) and night (6 h). Participants reported 5115 subjective place experiences (ranging from 160 to 120 per week) and seasonal participation, although declining, is accurate. Our findings indicate that the amount of data collected through smartphone sensors, fitness wristbands and in-app questionnaires is consistent enough to be leveraged for integrated assessments of habits, environmental exposure, and subjective and physiological well-being. Yet, considerable variation exists across individuals; thus diagnostic analysis must precede use of these datasets in any particular research endeavors. By doing so we can maximise the potential of ReaLM research to delve into real life conditions conducive to healthy living habits while also considering broader sustainability goals.

Analysis of pandemic outdoor recreation and green infrastructure in Nordic cities to enhance urban resilience.

Recent empirical research has confirmed the importance of green infrastructure and outdoor recreation to urban people's well-being during the COVID-19 pandemic. However, only a few studies provide cross-city analyses. We analyse outdoor recreation behaviour across four Nordic cities ranging from metropolitan areas to a middle-sized city. We collected map-based survey data from residents (n = 469-4992) in spring 2020 and spatially analyse green infrastructure near mapped outdoor recreation sites and respondents' places of residence. Our statistical examination reveals how the interplay among access to green infrastructure across cities and at respondents' residential location, together with respondents' socio-demographic profiles and lockdown policies or pandemic restrictions, affects outdoor recreation behaviour. The results highlight that for pandemic resilience, the history of Nordic spatial planning is important. To support well-being in exceptional situations as well as in the long term, green infrastructure planning should prioritise nature wedges in and close to cities and support small-scale green infrastructure.

Pro-environmental habits: An underexplored research agenda in sustainability science.

Habits are the fundamental basis for many of our daily actions and can be powerful barriers to behavioural change. Still, habits are not included in most narratives, theories, and interventions applied to sustainable behaviour. One reason societies struggle to reach policy goals and people fail to change towards more pro-environmental lifestyles might be that many behaviours are now bound by strong habits that override knowledge and intentions to act. In this perspective article, we provide three arguments for why pro-environmental habits are a needed research agenda in sustainability science: (1) habit theory highlights how behaviour is heavily reliant on automatic processes, (2) the environmental context sets boundary conditions for behaviour, shape habits, and cues action responses, and (3) our habits and past behaviour shape our values and self-identity. These arguments highlight the transformative potential of looking at sustainable behaviours through a habit lens. We believe a research agenda on pro-environmental habits could generate a more holistic understanding of sustainable behaviours and complement today's dominating approaches which emphasize reasoned decisions and intrinsic motivations such as values, norms, and intentions to understand and predict pro-environmental behaviour. We highlight evident knowledge gaps and practical benefits of considering habit theory to promote pro-environmental behaviours, and how habit architecture could be utilized as a strong leverage point when designing, modifying, and building urban environments.

Effect of Honey and Syrup Diets Enriched with 1,3-1,6 ß-Glucans on Honeybee Survival Rate and Phenoloxidase Activity (Apis mellifera L. 1758).

ß-glucans can activate the animal innate immune system by acting as immune-modulators and inducing various stimulatory effects. The aim of this study was to investigate the effect of 1,3-1,6 ß-glucans administered orally for 96 h on Apis mellifera workers (newly emerged and nurse bees). ß-glucans were included in honey and syrup. Survival rate and phenoloxidase activity were measured. In both newly emerged and nurse bees, ß-glucans supplementation did not affect survival rate (p > 0.05). Conversely, phenoloxidase activity was higher in both newly emerged bees (p = 0.048) and nurse bees (p = 0.014) fed with a honey diet enriched with ß-glucans compared to those fed with only honey. In both the newly emerged and nurse bees, no statistical differences in phenoloxidase activity were recorded between the group fed with a syrup-based diet enriched with ß-glucans and the control group (p > 0.05). The absence of significant variation in survival suggests that the potential negative effect of ß-glucans in healthy bees could be mitigated by their metabolism. Conversely, the inclusion of ß-glucans in a honey-based diet determined an increase of phenoloxidase activity, suggesting that the effect of ß-glucan inclusion in the diet of healthy bees on phenoloxidase activity could be linked to the type of base-diet. Further investigations on ß-glucans metabolism in bees, on molecular mechanism of phenoloxidase activation by 1,3-1,6 ß-glucans, and relative thresholds are desirable. Moreover, investigation on the combined action of honey and ß-glucans on phenoloxidase activity are needed.

Visiting nearby natural settings supported wellbeing during Sweden's "soft-touch" pandemic restrictions.

The coronavirus pandemic entailed varying restrictions on access, movement and social behavior in populations around the world. Knowledge about how people coped with "soft-touch" restrictions can inform urban spatial planning strategies that enhance resilience against future pandemics. We analyzed data from an online place-based survey on 2845 places across Sweden that respondents abstained from visiting, visited with similar frequency, or visited more frequently in spring 2020 as compared to before the pandemic. In spatial logistic regression models, we relate geographical and sociodemographic properties of places (fields, forests, water, residential population density and daytime population density) to self-perceived changes in wellbeing from visiting the given place less or more often, respectively. Abstaining from visiting places with natural features located in areas of high residential density was associated with a self-perceived negative influence on wellbeing. Yet, fields, forests and water were strongly associated with places people claimed wellbeing benefits from during pandemic restrictions. The further a visited place was from the respondent's home, the more likely it was to have a positive wellbeing influence. As an illustrative case, we map our models onto the landscape of Stockholm, showing that some neighborhoods are likely more resilient than others when coping with pandemic restrictions. Both the most and least resilient neighborhoods span the socio-economic spectrum. Urban planning will do well to enable equitable, easy access to natural settings by foot or bike, to increase pandemic preparedness as well as support climate change mitigation and biodiversity protection.

The regenerative compatibility: A synergy between healthy ecosystems, environmental attitudes, and restorative experiences.

Urban nature is and will be the most common provider of nature interactions for humankind. The restorative benefits of nature exposure are renown and creating human habitats that simultaneously support people's wellbeing and ecological sustainability is an urgent priority. In this study, we investigate how the relationship between environmental attitudes and healthy ecosystems influences restorative experiences combining a place-based online survey with geographical data on ecosystem health in Stockholm (Sweden). Using spatial regression, we predict the 544 restorative experiences (from 325 respondents), with people's environmental attitudes, natural land covers, ecosystem health, and the statistical interactions among these variables as predictors. Our results show that restorative experiences can happen anywhere in the urban landscape, but when they occur in natural environments, the combined levels of biodiversity and ecological connectivity are better predicting factor than the mere presence of nature. That is, healthy ecosystems seem to be more important than just any nature for restorative experiences. Moreover, the statistical interaction between one's environmental attitudes and natural environments predict almost all restorative experiences better than when these variables are independent predictors. This suggests that there is synergistic compatibility between environmental attitudes and healthy ecosystems that triggers restorative processes. We call this synergy regenerative compatibility. Regenerative compatibility is an unexploited potential that emerges when people's attitudes and ecosystems are aligned in sustainability. We consider regenerative compatibility a valuable leverage point to transform towards ecologically sustainable and healthy urban systems. To this end, we encourage multifaceted policy interventions that regenerate human-nature relationships holistically rather than implement atomistic solutions.

Human-nature relationships in context. Experiential, psychological, and contextual dimensions that shape children's desire to protect nature.

What relationship with nature shapes children's desire to protect the environment? This study crosses conventional disciplinary boundaries to explore this question. I use qualitative and quantitative methods to analyse experiential, psychological, and contextual dimensions of Human-Nature Connection (HNC) before and after children participate in a project of nature conservation. The results from the interviews (N = 25) suggest that experiential aspects of saving animals enhance children's appreciation and understanding for animals, nature, and nature conservation. However, the analysis of children's psychological HNC (N = 158) shows no statistical difference before and after children participate in the project. Analysing the third dimension-children's contextual HNC-provides further insights. Including children's contextual relations with home, nature, and city, not only improves the prediction of their desire to work for nature, but also exposes a form of Human-Nature Disconnection (HND) shaped by children's closeness to cities that negatively influence it. Overall, combining experiential, psychological, and contextual dimensions of HNC provides rich insights to advance the conceptualisation and assessment of human-nature relationships. People's relationship with nature is better conceived and analysed as systems of relations between mind, body, culture, and environment, which progress through complex dynamics. Future assessments of HNC and HND would benefit from short-term qualitative and long-term quantitative evaluations that explicitly acknowledge their spatial and cultural contexts. This approach would offer novel and valuable insights to promote the psychological and social determinants of resilient sustainable society.

Nosema ceranae infection in honeybee samples from Tuscanian Archipelago (Central Italy) investigated by two qPCR methods.

Nosema apis and Nosema ceranae are microsporidian parasite worldwide spread causing an emerging infectious disease of European honeybee Apis mellifera. The Nosema presence was deeply investigated in several countries but low information are presents about islands. In this investigation was evaluated the presence N. ceranae and N. apis in apiaries located in Tuscanian Archipelago islands (Central Italy). For N. ceranae detection, two different Real-Time PCR (qPCR) methods, the 16S rRNA and Hsp70 gene amplification qPCR, were performed on honey bee samples; while, for N. apis only the 16S rRNA qPCR amplification was performed. On all islands, only N. ceranae was present, while N. apis was not found in the samples. The two qPCR showed significant difference (p < 0.040) in N. ceranae spores quantification. The single-copy Hsp70 gene method qPCR assay systematically detected a lower amount of N. ceranae copies compared to the multi-copy 16S rRNA gene method.

The undisciplinary journey: early-career perspectives in sustainability science.

The establishment of interdisciplinary Master's and PhD programs in sustainability science is opening up an exciting arena filled with opportunities for early-career scholars to address pressing sustainability challenges. However, embarking upon an interdisciplinary endeavor as an early-career scholar poses a unique set of challenges: to develop an individual scientific identity and a strong and specific methodological skill-set, while at the same time gaining the ability to understand and communicate between different epistemologies. Here, we explore the challenges and opportunities that emerge from a new kind of interdisciplinary journey, which we describe as 'undisciplinary.' Undisciplinary describes (1) the space or condition of early-career researchers with early interdisciplinary backgrounds, (2) the process of the journey, and (3) the orientation which aids scholars to address the complex nature of today's sustainability challenges. The undisciplinary journey is an iterative and reflexive process of balancing methodological groundedness and epistemological agility to engage in rigorous sustainability science. The paper draws upon insights from a collective journey of broad discussion, reflection, and learning, including a survey on educational backgrounds of different generations of sustainability scholars, participatory forum theater, and a panel discussion at the Resilience 2014 conference (Montpellier, France). Based on the results from this diversity of methods, we suggest that there is now a new and distinct generation of sustainability scholars that start their careers with interdisciplinary training, as opposed to only engaging in interdisciplinary research once strong disciplinary foundations have been built. We further identify methodological groundedness and epistemological agility as guiding competencies to become capable sustainability scientists and discuss the implications of an undisciplinary journey in the current institutional context of universities and research centers. In this paper, we propose a simple framework to help early-career sustainability scholars and well-established scientists successfully navigate what can sometimes be an uncomfortable space in education and research, with the ultimate aim of producing and engaging in rigorous and impactful sustainability science.

Fostering Children's Connection to Nature Through Authentic Situations: The Case of Saving Salamanders at School.

The aim of this paper is to explore how children learn to form new relationships with nature. It draws on a longitudinal case study of children participating in a stewardship project involving the conservation of salamanders during the school day in Stockholm, Sweden. The qualitative method includes two waves of data collection: when a group of 10-year-old children participated in the project (2015) and 2 years after they participated (2017). We conducted 49 interviews with children as well as using participant observations and questionnaires. We found indications that children developed sympathy for salamanders and increased concern and care for nature, and that such relationships persisted 2 years after participation. Our rich qualitative data suggest that whole situations of sufficient unpredictability triggering free exploration of the area, direct sensory contact and significant experiences of interacting with a species were important for children's development of affective relationships with the salamander species and with nature in an open-ended sense. Saving the lives of trapped animals enabled direct sensory interaction, feedback, increased understanding, and development of new skills for dynamically exploring further ways of saving species in an interactive process experienced as deeply meaningful, enjoyable and connecting. The behavioral setting instilled a sense of pride and commitment, and the high degree of responsibility given to the children while exploring the habitat during authentic situations enriched children's enjoyment. The study has implications for the design of education programs that aim to connect children with nature and for a child-sensitive urban policy that supports authentic nature situations in close spatial proximity to preschools and schools.

Proteinase pattern of honeybee prepupae from healthy and American Foulbrood infected bees investigated by zymography.

American foulbrood disease (AFB) is the main devastating disease that affects honeybees' brood, caused by Paenibacillus larvae. The trend of the research on AFB has addressed the mechanisms by which P. larvae bacteria kill honeybee larvae. Since prepupae could react to the infection of AFB by increasing protease synthesis, the aim of this work was to compare protease activity in worker prepupae belonging to healthy colonies and to colonies affected by AFB. This investigation was performed by zymography. In gel, proteolytic activity was observed in prepupae extracts belonging only to the healthy colonies. In the prepupae extracts, 2D zimography followed by protein identification by MS allowed to detect Trypsin-1 and Chymotrypsin-1, which were not observed in diseased specimens. Further investigations are needed to clarify the involvement of these proteinases in the immune response of honeybee larvae and the mechanisms by which P. larvae inhibits protease production in its host.

A Framework to Assess Where and How Children Connect to Nature.

The design of the green infrastructure in urban areas largely ignores how people's relation to nature, or human-nature connection (HNC), can be nurtured. One practical reason for this is the lack of a framework to guide the assessment of where people, and more importantly children, experience significant nature situations and establish nature routines. This paper develops such a framework. We employed a mixed-method approach to understand what qualities of nature situations connect children to nature (RQ1), what constitutes children's HNC (RQ2), and how significant nature situations and children's HNC relate to each other over time (RQ3). We first interviewed professionals in the field of connecting children to nature (N = 26), performed inductive thematic analysis of these interviews, and then further examined the inductive findings by surveying specialists (N = 275). We identified 16 qualities of significant nature situations (e.g., "awe," "engagement of senses," "involvement of mentors") and 10 abilities that constitute children's HNC (e.g., "feeling comfortable in natural spaces," "feeling attached to natural spaces," "taking care of nature"). We elaborated three principles to answer our research questions: (1) significant nature situations are various and with differing consequences for children's HNC; (2) children's HNC is a complex embodied ability; (3) children's HNC progresses over time through diverse nature routines. Together, these findings form the Assessment framework for Children's Human Nature Situations (ACHUNAS). ACHUNAS is a comprehensive framework that outlines what to quantify or qualify when assessing "child-nature connecting" environments. It guides the assessment of where and how children connect to nature, stimulating both the design of nature-connecting human habitats as well as pedagogical approaches to HNC.

Infectivity of DWV associated to flower pollen: experimental evidence of a horizontal transmission route.

Deformed wing virus (DWV) is a honeybee pathogen whose presence is generally associated with infestation of the colony by the mite Varroa destructor, leading to the onset of infections responsible for the collapse of the bee colony. DWV contaminates bee products such as royal jelly, bee-bread and honey stored within the infected hive. Outside the hive, DWV has been found in pollen loads collected directly from infected as well as uninfected forager bees. It has been shown that the introduction of virus-contaminated pollen into a DWV-free hive results in the production of virus-contaminated food, whose role in the development of infected bees from virus-free eggs has been experimentally demonstrated. The aim of this study was twofold: (i) to ascertain the presence of DWV on pollen collected directly from flowers visited by honeybees and then quantify the viral load and (ii) determine whether the virus associated with pollen is infective. The results of our investigation provide evidence that DWV is present on pollen sampled directly from visited flowers and that, following injection in individuals belonging to the pollinator species Apis mellifera, it is able to establish an active infection, as indicated by the presence of replicating virus in the head of the injected bees. We also provide the first indication that the pollinator species Osmia cornuta is susceptible to DWV infection.