RESUMEN
Dermatophilus-like bacteria were observed in histological examinations of samples of diseased foot skin from greater flamingos (Phoenicopterus roseus) living in zoological gardens in Switzerland. When grown on TSA-SB containing polymyxin B, the bacteria isolated from these skin samples formed hyphae, as is typical for Dermatophilus congolensis, but these bacteria were non-haemolytic. The closest relatives based on 16S rRNA gene sequences were the two members of the genus Arsenicicoccus, Arsenicicoccus bolidensis and Arsenicicoccus piscis. A representative of the isolated strains shared 34.3â% DNA-DNA relatedness with the type strain of A. bolidensis, 32.3â% with the type strain of A. piscis and 34.5â% with the type strain of D. congolensis, demonstrating that these strains do not belong to any of these species. The phenotypic characteristics differed from those of members of the genus Arsenicicoccus as well as from those of D. congolensis. The G+C content of strain KM 894/11(T) was 71.6 mol%. The most abundant fatty acids were iso-C15â:â0, summed feature 3 (including C16â:â1ω7c and/or iso-C15â:â0 2-OH) and C18â:â1ω9c. MK-8(H4) was the predominant menaquinone. Cell-wall structure analysis revealed that the peptidoglycan type was A3γ ll-Dpm-Gly (type A41.1). Based on genotypic and chemotaxonomic characteristics, the isolated strains represent a novel species within the genus Arsenicicoccus, for which the name Arsenicicoccus dermatophilus sp. nov. is proposed. The type strain is KM 894/11(T) (â=âDSM 25571(T)â=âCCUG 62181(T)â=âCCOS 690(T)), and strain KM 1/12 (â=âDSM 25572â=âCCUG 62182â=âCCOS 691) is a reference strain.
Asunto(s)
Actinomycetales/clasificación , Enfermedades de las Aves/microbiología , Aves/microbiología , Filogenia , Enfermedades Cutáneas Bacterianas/veterinaria , Piel/microbiología , Actinomycetales/genética , Actinomycetales/aislamiento & purificación , Animales , Animales de Zoológico/microbiología , Técnicas de Tipificación Bacteriana , Composición de Base , Pared Celular/química , ADN Bacteriano/genética , Ácidos Grasos/química , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Peptidoglicano/química , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Enfermedades Cutáneas Bacterianas/microbiología , Suiza , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMEN
Gram-negative, aerobic, motile, rod-shaped bacteria were isolated from the intestines of freshwater fish on two separate occasions. Colonies of both strains, JF3835(T) and JF4413, produced non-diffusible green pigment following 4-5 days incubation on Luria-Bertani agar. The most abundant fatty acids were summed feature 3 (comprising C(16â:â1)ω7c and/or C(15â:â0) iso 2-OH), C(16â:â0) and C(18â:â1)ω7c. The DNA G+C content was 62.9 mol%. Sequence analysis of the 16S rRNA gene indicated 100â% sequence similarity between the two strains. In comparison with recognized species, the new strains exhibited the greatest degree of sequence similarity with members of the Pseudomonas chlororaphis subspecies: P. chlororaphis subsp. chlororaphis (99.84â%), P. chlororaphis subsp. aurantiaca (99.75â%) and P. chlororaphis subsp. aureofaciens (99.40â%). While DNA-DNA relatedness confirmed the placement of strains JF3835(T) and JF4413 as members of the species P. chlororaphis, multilocus sequencing indicated that the strains formed a distinct cluster within it. On the basis of genotypic and phenotypic evidence, strains JF3835(T) and JF4413 represent a novel subspecies of the species P. chlororaphis, for which the name Pseudomonas chlororaphis subsp. piscium subsp. nov. is proposed. The type strain is JF3835(T) (=NCIMB 14478(T)=DSM 21509(T)).