Copper sulfate induced toxicological impact on in-vivo zebrafish larval model protected due to acacetin via anti-inflammatory and glutathione redox mechanism.

Copper sulfate (CuSO4) as industrial effluent is intentionally or unintentionally released into water bodies and accumulates in the fish. Because of its numerous applications, CuSO4 can be hazardous to non-target creatures, producing direct alterations in fish habitats. Acacetin is a flavonoid present in all vascular plants that are extensively dispersed in plant pigments and responsible for many natural hues. However, the impact of acacetin on mitigating the toxic effect of CuSO4 in the in-vivo conditions is not known. The toxicity of acacetin was determined by measuring the survival, deformities and heart rate after treatment with various concentrations to larvae. The protective effect of acacetin was also observed in CuSO4 exposed zebrafish larvae by reducing malformation, mortality rate and oxidative stress. Meanwhile, the acacetin-protected larvae from CuSO4 effects through the molecular mechanism by suppressing pro-inflammatory genes (COX-2, TNF-α and IL-1) and upregulating antioxidant genes (GPx, GST and GR). Overall, our findings suggest that acacetin can act as a protective barrier against CuSO4-induced inflammation in an in-vivo zebrafish larval model.

Enhancement of biogas production from microalgal biomass through cellulolytic bacterial pretreatment.

Generation of bioenergy from microalgal biomass has been a focus of interest in recent years. The recalcitrant nature of microalgal biomass owing to its high cellulose content limits methane generation. Thus, the present study investigates the effect of bacterial-based biological pretreatment on liquefaction of the microalga Chlorella vulgaris prior to anaerobic biodegradation to gain insights into energy efficient biomethanation. Liquefaction of microalgae resulted in a higher biomass stress index of about 18% in the experimental (pretreated with cellulose-secreting bacteria) vs. 11.8% in the control (non-pretreated) group. Mathematical modelling of the biomethanation studies implied that bacterial pretreatment had a greater influence on sustainable methane recovery, with a methane yield of about 0.08 (g Chemical Oxygen Demand/g Chemical Oxygen Demand), than did control pretreatment, with a yield of 0.04 (g Chemical Oxygen Demand/g Chemical Oxygen Demand). Energetic analysis of the proposed method of pretreatment showed a positive energy ratio of 1.04.