Assessment of Malondialdehyde and Organochlorine Pesticides in Aplastic Anemia Severity Groups: Insights Into Oxidative Stress and Exposure.

Background There is little evidence that pesticide exposure is the primary cause of acquired aplastic anemia (AAA), even though the prevalence of aplastic anemia (AA) is substantially higher in underdeveloped countries than in affluent countries. AA caused by pesticides has not yet been fully understood. This study aimed to examine the potential link between plasma levels of malondialdehyde (MDA) and organochlorine pesticides (OCPs) as risk factors for developing AAA in the North Indian population. Methods This case-control study was conducted at a tertiary care hospital in North India. A total of 99 participants were chosen for the study, of whom 45 were cases of AA. These cases attended the Clinical Hematology department over a period of 1.5 years (May 2018 to November 2019). Forty-five controls were age and sex-matched, apparently healthy subjects. Written informed consent was obtained from each subject before performing the study. Exclusion criteria included patients unwilling to give consent, those using medication to treat AA, those genetically predisposed to AA, those with characteristics including granuloma and dysplasia of bone marrow, any other systemic illness, and subjects with a history of smoking, drinking, or using tobacco in any form. Gas chromatography-tandem mass spectrometry (GC-MS/MS) was used to evaluate the plasma levels of organochlorines. The estimation of plasma MDA, i.e., the lipid peroxide content, was measured. Results The severity of AA is significantly associated with plasma levels of α-Hexachlorocyclohexane (p = 0.040), Heptachlor (p = 0.006), Aldrin (p < 0.001), p,p'-Dichlorodiphenyldichloroethane (p = 0.004), Endosulfan sulfate (p = 0.010), and Methoxychlor (p = 0.001). There was a statistically non-significant difference in MDA levels between cases and controls (p = 0.145); however, a statistically significant linear increase in MDA levels (p < 0.001) was observed according to the severity of AA. Conclusion Our study suggests that oxidative stress may be linked to the severity of AA. Pesticide exposure (plasma organochlorine levels) could act as a stressor, potentially initiating an alarmin response of oxidative stress in the form of lipid peroxidation (MDA) from damaged tissue, which could then lead to suppression of hematopoiesis and be a possible factor in the development of AA.

Plasma Levels of Organochlorine Pesticides and Tumor Necrosis Factor-Alpha: A Potential Risk Factor for Developing Acquired Aplastic Anemia in the North Indian Population.

Background Pesticide exposure might have a contributory role in the development of acquired aplastic anemia (AA). However, the precise mechanisms of pesticide-induced AA remain unknown. In this case-control study, we conducted a comparative analysis of plasma levels of organochlorine pesticides (OCP) and tumor necrosis factor-alpha (TNF-alpha) between Indian patients diagnosed with AA and an age- and sex-matched control group. Methods This is an observational case-control study conducted at a tertiary care hospital in North India. In this study, 90 subjects were included, out of which 45 were diagnosed with AA according to the criteria of the International Agranulocytosis and Aplastic Anemia Study. Cases were compared with 45 controls. A trained interviewer gave all study subjects a questionnaire to collect data regarding demographic details, exposure to pesticides, and clinical history. Physical examination and routine laboratory investigations of each subject were performed. Both cases and controls were tested for their plasma levels of organochlorines as per established protocol by gas chromatography-mass spectrometry. TNF-alpha level was measured by enzyme-linked immunosorbent assay in each subject. Results There was a significant increase in plasma levels of delta hexachlorocyclohexane (delta HCH) (p = 0.02) and heptachlor (p = 0.00) in patients with AA as compared to controls. We observed nonsignificant trends towards higher levels of beta HCH (p = 0.643), aldrin (p = 0.399), and p,p'-Dichlorodiphenyltrichloroethane (p,p'-DDT) (p = 0.453) in patients with AA when compared to the controls. There were significantly higher TNF-alpha levels (p = 0.024) in cases as compared to the controls. Conclusion Our study concludes that patients with AA exhibited higher levels of delta-HCH, heptachlor, and TNF-alpha in comparison to the control group. There is a significant positive correlation of TNF alpha with OCPs (alpha HCH, lindane, delta HCH, heptachlor, aldrin, p,p'- DDD, and methoxychlor pesticides). These organochlorines may have accumulated in the fatty tissue of bone marrow because of their lipophilic nature. This suggests that they might have served as a neoantigen to trigger an increase in TNF-alpha production, which may have led to disrupted bone marrow function through cell-mediated immunity, leading to AA.

Antiresonant hollow-core fiber Bragg grating design.

Fiber Bragg gratings (FBGs) inscribed in hollow-core fibers hold a potential to revolutionize the field of gas photonics by enhancing the performance and versatility of hollow-core fiber-based matter cells. By effectively transforming these cells into cavities, FBGs can significantly extend the effective length of light-matter interactions. Traditional FBG inscription methods cannot be extended to hollow-core fibers, because light in the fundamental mode is predominantly confined to the hollow region where an index change cannot be induced. In this Letter, we propose a bi-thickness dual-ring hollow-core antiresonant fiber (DRHCF) design that achieves substantial overlap between the fundamental mode and cladding glass in a well-controlled manner, ensuring a strong FBG response with a minimal insertion loss. Through detailed numerical investigations, we demonstrate the feasibility of creating a high reflection FBG in the DRHCF using standard FBG inscription techniques. The proposed device is expected to have a length of <1 cm and the insertion loss of <0.3 dB, including splice loss.

Linearly polarized ytterbium laser enabled by an antiresonant hollow-core fiber inline polarizer.

We report a linearly polarized ytterbium-doped fiber (YDF) laser cavity configured by integrating an antiresonant hollow-core fiber-based inline polarizer. The 5-cm-long compact fiber polarizer was fusion spliced to a commercial large-mode-area, polarization-maintaining YDF. Near-diffraction-limited linearly polarized signal output with a polarization extinction ratio of > 21 dB was achieved for up to 25 W of power that was limited only by the available pump power. The performance of the hollow-core fiber polarizer was found to be temperature insensitive, which obviates the need for the precise temperature control required in all-fiber, high-power polarized laser cavities employing crossed fiber Bragg gratings. We used the tapering technique to scale down the geometry of the polarizing fiber and shift its operating wavelength by ∼100 nm, which makes it an attractive candidate for a variety of fiber laser applications.

Influence of processing on bioactive compounds, Type-II diabetes related enzyme regulation potential and antiurolithiatic potential of underutilized legume Macrotyloma uniflorum.

The hydro-alcoholic extract of raw and processed Macrotyloma uniflorum seeds, an underutilized food legume was analysed for its bioactive compounds, Type-II diabetes enzyme regulation and antiurolithiatic potential. The study aimed to establish and promote the introduction of these new grains and enlarge the market of novel functional foods. The seed extract had phenolic content of 35.6 and 30.4 mg GAE/g dm, for PAIYUR-2 and GPM-6 respectively. Chlorogenic, coumaric, vanillic and ellagic were the major and, sinapic and syringic were limiting phenolic acids. The raw seeds extract exhibited ferric ion reducing potential (1125 and 1236 mmol Fe II/mg extract dm), free radical inhibition (EC50, 3.58 and 3.78 g dm/g DPPH), hydroxyl ion inhibition (46.71 and 45.44%) and superoxide ion inhibition (36.93 and 33.37%) for PAIYUR-2 and GPM-6 respectively. Further, considerable α-amylase (49.34 & 45.89%) and α-glucosidase (62.72 & 60.33%) inhibition potentials were observed along with antiurolithiatic activity of 48.12 and 46.31% in PAIYUR-2 and GPM-6 respectively. Although, processing had significant (p ≤ 0.05) impact on grain quality but still the significant (p ≤ 0.05) functional properties were retained. This proves the grain utility as a functional food in maintaining human health.

Antibiofilm Potential of Alpha-Amylase from a Marine Bacterium, Pantoea agglomerans.

Bacterial biofilms are a big menace to industries and the environment and also in the health sector, accumulation of which is a major challenge. Despite intensive efforts to curb this issue, a definitive solution is yet to be achieved. Enzyme-templated disruption of the extracellular matrix of biofilm and its control and elimination are emerging as an efficient and greener strategy. The study describes the antibiofilm potential of alpha-amylase from the marine microorganism Pantoea agglomerans PCI05, against food-borne pathogens. Amylase exhibited stability in a wide pH range and retained 50% of its activity at temperatures as high as 100°C. Thermal analysis of the enzyme produced showed thermal stability, up to 130°C. From these findings, it can be envisaged that the alpha-amylase produced from P. agglomerans can be used for starch liquefaction; it was also evaluated for antibiofilm activity. Amylase from this marine bacterium was found to efficiently disrupt the preformed biofilms of food-borne pathogens such as Bacillus cereus, Serratia marcescens, Vibrio parahaemolyticus, Listeria monocytogenes, and Salmonella enterica enterica serotype Typhi based on the value of biofilm inhibitory concentrations.

All-solid antiresonant fiber design for high-efficiency three-level lasing in ytterbium-doped fiber lasers.

We propose and investigate an all-solid ytterbium-doped antiresonant fiber (YbARF) design to inherently suppress four-level lasing with >20 dB/m of selective loss and achieve high-efficiency three-level lasing while maintaining near-diffraction-limited operation with an ultra-large mode area of approximately 3630 µm2. The YbARF is designed such that the high-gain wavelengths corresponding to four-level lasing lie in the resonance band characterized by high confinement loss. This enables three-level lasing with high efficiency in a short (0.8-m-long) YbARF, making it a potential candidate for high-peak-power ultrafast lasers at 976 nm. We discuss fiber design considerations and detailed simulation results for three-level lasing performance in the YbARF, which promises >85% lasing efficiency in a single-pass pump configuration. These design concepts can be easily extended to suppress high-gain wavelengths in other rare-earth-doped (e.g., with thulium, erbium, and neodymium) fiber amplifiers or lasers.

Integration of an anti-resonant hollow-core fiber with a multimode Yb-doped fiber for high power near-diffraction-limited laser operation.

We proposed and demonstrated mode cleaning in a high-power fiber laser by integrating an anti-resonant hollow-core fiber (AR-HCF) into a multimode laser cavity of an ytterbium (Yb)-doped fiber (YDF). An in-house mode-matched AR-HCF was fusion-spliced to a commercial multimode LMA-YDF, ensuring efficient fundamental mode coupling. The AR-HCF inflicts a high propagation loss selectively on higher-order modes, facilitating fundamental mode operation. Thus, the AR-HCF works as an efficient spatial mode filter embedded in the multimode fiber laser cavity and reinforces preferential amplification of the fundamental mode. Beam quality factor enhancement was achieved from M2 = 2.09 to 1.39 at an output power of 57.7 W (pump-power limited). The beam quality can be further improved by refining the AR-HCF fabrication. The proposed technique has a great potential to be exploited in other multimode fiber laser cavities involving erbium- or thulium-doped fibers and obviates the need for complicated specialty active fiber designs. Compared with the commonly used fiber bending technique, our method can achieve an efficient higher-order mode suppression without inducing mode-field deterioration.

Anti-resonant hollow-core fiber fusion spliced to laser gain fiber for high-power beam delivery.

We present the selective excitation of the fundamental mode in an anti-resonant hollow-core fiber (ARHCF) fusion-spliced with a commercial large mode area (LMA) fiber. By designing and fabricating a single-ring ARHCF that is mode-matched to a LMA fiber and by splicing the two using a CO2 laser-based splicer, we achieve a coupling efficiency of 91.2% into the fundamental mode. We also demonstrate an all-fiber integration of an ARHCF with a commercial ytterbium-doped fiber in a laser cavity for beam delivery application. Coupling of the single-mode laser output beam into the fundamental mode of the ARHCF is demonstrated with 90.4% efficiency (<0.45dB loss) for up to 50 W continuous wave beam in a stable and alignment-free all-fiber laser setup.

Large-mode-area multicore Yb-doped fiber for an efficient high power 976 nm laser.

We present an efficient 976 nm laser generation from an ytterbium (Yb)-doped step-index multicore fiber (MCF) with six cores placed in a ring shape. Each of the six cores has a large-mode-area (LMA) and a low numerical aperture (NA), which makes the MCF equipped with the features of a large core-to-cladding area ratio and differential bending loss for wavelength and mode selection. Hence, the Yb-doped MCF benefits 976 nm laser generation by simultaneously suppressing unwanted 1030 nm emission and higher-order modes (HOMs). A 976 nm laser is obtained in a short piece (88 cm) of the Yb MCF, with a good slope efficiency of 46% with respect to launched pump power and the maximum output power of 25 W (pump power limited). A mode area of 1432 µm2 at the 976 nm is expected for the fundamental in-phase mode.

Physical modification of starch: changes in glycemic index, starch fractions, physicochemical and functional properties of heat-moisture treated buckwheat starch.

Native buckwheat starch was extracted and modified by heat-moisture treatment (HMT) with different treatment time (15, 30 and 45 min) to investigate its effect on physicochemical, morphological, functional properties, starch profile (rapidly digestible starch, RDS; slowly digestible starch, SDS and resistant starch, RS fractions) and expected glycemic index (eGI). Results revealed that with increasing time duration of HMT from 15 to 45 min, amylose content, pasting temperature and thermostability increased substantially whereas swelling power, solubility and viscosity parameters decreased. The SEM micrographs showed that HMT caused fissures in the granule and surface indentation. HMT-45 (starch treated for 45 min) had the lowest RDS content (29.33%) and the highest SDS (51.30%) and RS (8.21%) levels. The decreased hydrolysis rate, high amylose and RS content of HMT-45 resulted in a significant decrease in estimated glycemic index (eGI) values from 51.49% (Native) to 44.16% (HMT-45) thus indicating its role in prevention of non-insulin- dependent diabetes.

Development and storage stability of buckwheat chips using response surface methodology (RSM).

Processing conditions (potato level, frying temperature and frying time) were optimized for the development of buckwheat based chips using response surface methodology (RSM). Moisture content, oil uptake, color values, hardness and overall acceptability (OAA) were used as indices of product quality. The polynomial regression model was fitted with R2 values of 0.983, 0.982, 0.98, 0.996, 0.973, 0.984 and 0.985 for moisture content, oil uptake, L, a*, b* values, hardness, and OAA respectively indicating fitness of the models. Potato level and frying temperature showed a significant effect on all responses at linear and quadratic levels except frying temperature for OAA at the linear level and hardness at quadratic level. Frying time showed significant effect on a* value, b* value, hardness and OAA at linear level. Interaction between all processing variables had a significant effect on a* value. Interaction between potato level and frying temperature had significant negative effect on moisture content of buckwheat chips. Potato level (30%), frying temperature (169 °C) and frying time (51 s) were found to be the optimum processing conditions with maximum OAA (8.36). 0.33 aw was established as the water activity at which maximum stability of chips was shown. Buckwheat chips packed in both polypropylene (PP) and metallised polyester (MP) remained stable and acceptable for 6 months at RT whereas for 3 and 6 months in PP and MP films respectively at 37 °C.