RESUMEN
The ability to measure biomarkers, both specifically and selectively at the single-molecule level in biological fluids, has the potential to transform the diagnosis, monitoring, and therapeutic intervention of diseases. The use of nanopores has been gaining prominence in this area, not only for sequencing but more recently in screening applications. The selectivity of nanopore sensing can be substantially improved with the use of labels, but substantial challenges remain, especially when trying to differentiate between bound from unbound targets. Here highly sensitive and selective molecular probes made from nanoparticles (NPs) that self-assemble and dimerize upon binding to a biological target are designed. It is shown that both single and paired NPs can be successfully resolved and detected at the single-molecule nanopore sensing and can be used for applications such as antigen/antibody detection and microRNA (miRNA) sequence analysis. It is expected that such technology will contribute significantly to developing highly sensitive and selective strategies for the diagnosis and screening of diseases without the need for sample processing or amplification while requiring minimal sample volume.
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Nanotecnología , Técnicas Biosensibles , MicroARNs , NanoporosRESUMEN
The dynamics of evaporating water droplets on heated graphene-poly(dimethylsiloxane) (PDMS) composites is investigated experimentally and theoretically. By inserting graphene nucleates in PDMS, we report the effect of change in thermal resistance on the evaporation process of water droplets on the heated graphene-PDMS composite surface. By dispersing graphene within the PDMS matrix, the evaporation of water droplets is enhanced. The graphene nucleate density over the surface was controlled by varying graphene wt % from 0 to 2%, which in turn controls the thermal resistance and hence the evaporation rate. Experimentally, the maximum evaporation rate of 0.0044 µL/s was observed for the sample of 2 wt % graphene-PDMS composite. The evaporation rate on a 2 wt % graphene-PDMS composite surface is about 1.5 times higher compared to that of plain PDMS without graphene. A theoretical model confirms that the initial contact angle and the presence of thermal coupling between liquid droplets and the substrate play an important role in evaporation dynamics. Thermal conductance increases 3 times with the increase in graphene wt % from 0.1 to 2.0 wt % in PDMS. The heat-storing capacity of graphene is responsible for the enhanced evaporation. The experimental findings are in good agreement with theoretical results. These samples were found insensitive to degradation and may find potential applications where high efficiency and high heat flux are needed.
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Our results demonstrate that it is possible, by means of generation of buckles on the silver nanorod (AgNR) array PDMS substrate, to enhance the Raman signal of P. aeruginosa bacteria due to the formation of high density 'hot spots' among the AgNR arrays which provides better entrapment and increases the net effective contact area of bacteria with the metal surface.
Asunto(s)
Técnicas Biosensibles , Dimetilpolisiloxanos/química , Nanotubos/química , Pseudomonas aeruginosa/aislamiento & purificación , Plata/química , Espectrometría RamanRESUMEN
We report the fabrication of anisotropic superhydrophobic surface with dual-scale roughness by the deposition of silver nanorods arrays on prestretched poly(dimethylsiloxane) (PDMS) using oblique angle deposition and subsequent release of strain after the deposition, which resulted in the formation of microbuckles/wrinkles. The amplitude and periodicity of the wrinkles were tuned by varying the prestretching mechanical strain (ε) applied to the PDMS film from 0 to 30% prior to Ag nanorods deposition. The peaks and valleys in the surface topography of Ag nanorods arrays covered PDMS films lead to anisotropic wetting by water droplet. The droplet is free to move along the direction parallel to the wrinkles, but the droplet moving perpendicular to the wrinkles confront energy barrier leading to wetting anisotropy. The anisotropic wettability was tuned from 22 to 37° for 10-30% prestretched PDMS film. The dual scale roughness (nanorods on micro wrinkles) was found to be responsible for the superhydrophobicity (contact angle â¼155°) of the sample prepared for 30% prestretched PDMS film in perpendicular direction. The wetting behavior of the Ag nanorods PDMS film surface was reversibly tuned by applying the mechanical strain, which induces the change in the microscale roughness determined by amplitude (A) and periodicity (λ) of the buckles. Most interestingly, the water droplet also displayed the anisotropy in the roll-off angle. The effect of different A and λ on anisotropic wettability of Ag nanorods arrays/PDMS film was also demonstrated by lattice Boltzmann (LB) modeling. These findings may produce a promising way of controlling the direction of liquid flow such as in microfluidic devices and transportation of the microliter water droplets in a preset direction.