RESUMEN
Supraphysiological ROS levels can lead to apoptosis, lipid peroxidation, and DNA and protein damage. This pilot study aimed to investigate the sperm oxidative damage in subfertile men, to describe the relationship between the antioxidant system and ROS. Sixty-four semen samples were categorised according to the evaluated routine parameters (WHO, WHO laboratory manual for the examination and processing of human semen, 2010). Results were cross-referenced with the DNA damage [Comet (n = 53) and TUNEL (n = 49) assays], antioxidant enzyme activity [SOD (n = 51), CAT (n = 48) and GST (n = 48)], and content of total thiols (n = 36), lipid hydroperoxides (n = 35) and MDA (n = 31). Compared to pathospermic samples, normozoospermic presented 40%-45% fewer spermatozoa with fragmented DNA, 19% fewer hydroperoxides, and slightly higher total thiols and MDA levels. Asthenozoospermic/asthenoteratozoospermic samples had the lowest GST activity. SOD and CAT showed a similar trend. Our results evidenced significant positive correlations between DNA damage and immotile spermatozoa; SOD and CAT, GST and total thiols; CAT and GST; total thiols and sperm concentration; and MDA levels and head/midpiece abnormalities and hydroperoxides. This work contributes to the existing body of knowledge by showing that the oxidative status correlates with the classic sperm analysis parameters. Oxidative stress and DNA damage evaluation might be a valuable diagnostic and prognostic tool in cases of idiopathic male subfertility.
Asunto(s)
Antioxidantes , Infertilidad Masculina , Antioxidantes/metabolismo , Daño del ADN , Humanos , Infertilidad Masculina/metabolismo , Masculino , Estrés Oxidativo , Proyectos Piloto , Semen , Espermatozoides/metabolismoRESUMEN
A cross-sectional pilot study was conducted in men followed in fertility consultations, from the portuguese Trás-os-Montes and Alto Douro region, in order to associate several lifestyle factors with the spermatic parameters. Of a total of 522 men, 373 were compared based on the occupational exposure to harmful factors, smoking habits and practice of physical exercise per week, and the other 149 men according to their body mass index (normal weight vs. overweight vs. obesity). In the absence of harmful occupational factors, physical exercise seems to be associated with sperm quality improvement, whether individuals smoke or not. When exposed to harmful environments, non-smokers that practice physical exercise more than two times per week tended to present the best vitality, normal morphology and sperm concentration (p > .05). However, if they smoke, physical exercise seems not enough to enhance the spermatic parameters. The BMI correlated negatively with the spermatic quality, especially with sperm concentration (p < .05). Concerning men that did not present lifestyle risks associated, the motility, midpiece and tail abnormalities, and teratozoospermia index were significantly worse on obese individuals comparing to overweight men (p < .05). Thus, patients should also be recommended to control their weight and to have a BMI under 30 kg/m2 .
Asunto(s)
Índice de Masa Corporal , Ejercicio Físico , Exposición Profesional/efectos adversos , Análisis de Semen/estadística & datos numéricos , Fumar/efectos adversos , Adulto , Humanos , Estilo de Vida , Masculino , Persona de Mediana Edad , Proyectos Piloto , Adulto JovenRESUMEN
Apresenta-se o conteúdo de Enfermagem Materno-Infantil, Enfermagem Neuro-Psiquiátrica, Educaçäo Moral e Cívica e Psicologia das Realaçöes Humanas e Etica, com os objetivos, as unidades, atividades e carga horária.
Asunto(s)
Asistentes de Enfermería , Curriculum , Educación en Enfermería , Prácticas ClínicasRESUMEN
Apresenta-se os conteúdos dos programas de Enfermagem Médica e cirúrgica, com seus objetivos, unidades, atividades e carga horária.
Asunto(s)
Asistentes de Enfermería , Curriculum , Educación en EnfermeríaRESUMEN
Apresenta-se o conteúdo de Fundamentos de Enfermagem com os objetivos, as unidades, atividades e carga horária.
