Metabolic Reprogramming in Gliocyte Post-cerebral Ischemia/ Reperfusion: From Pathophysiology to Therapeutic Potential.

Ischemic stroke is a leading cause of disability and death worldwide. However, the clinical efficacy of recanalization therapy as a preferred option is significantly hindered by reperfusion injury. The transformation between different phenotypes of gliocytes is closely associated with cerebral ischemia/ reperfusion injury (CI/RI). Moreover, gliocyte polarization induces metabolic reprogramming, which refers to the shift in gliocyte phenotype and the overall transformation of the metabolic network to compensate for energy demand and building block requirements during CI/RI caused by hypoxia, energy deficiency, and oxidative stress. Within microglia, the pro-inflammatory phenotype exhibits upregulated glycolysis, pentose phosphate pathway, fatty acid synthesis, and glutamine synthesis, whereas the anti-inflammatory phenotype demonstrates enhanced mitochondrial oxidative phosphorylation and fatty acid oxidation. Reactive astrocytes display increased glycolysis but impaired glycogenolysis and reduced glutamate uptake after CI/RI. There is mounting evidence suggesting that manipulation of energy metabolism homeostasis can induce microglial cells and astrocytes to switch from neurotoxic to neuroprotective phenotypes. A comprehensive understanding of underlying mechanisms and manipulation strategies targeting metabolic pathways could potentially enable gliocytes to be reprogrammed toward beneficial functions while opening new therapeutic avenues for CI/RI treatment. This review provides an overview of current insights into metabolic reprogramming mechanisms in microglia and astrocytes within the pathophysiological context of CI/RI, along with potential pharmacological targets. Herein, we emphasize the potential of metabolic reprogramming of gliocytes as a therapeutic target for CI/RI and aim to offer a novel perspective in the treatment of CI/RI.

High butanol/acetone ratio featured ABE production using mixture of glucose and waste Pichia pastoris medium-based butyrate fermentation supernatant.

In this study, butanol (ABE) fermentations were implemented in a 7 L anaerobic fermentor, by directly using the mixture of glucose solution with the corn/waste Pichia pastoris medium-based butyrate fermentation supernatants (BFS II) as the co-substrate, followed by consecutively feeding of the BFS and concentrated glucose solution. When compared with the major index of ABE fermentation using 150 g/L corn-based medium, butanol concentration could be maintained at high level of 12.7-12.8 g/L, butanol/acetone (B/A) largely increased from ~ 2.0 to 4.4-5.0, butanol yield on total carbon sources increased from 0.32-0.34 to 0.39-0.41 (mol base) with a higher butyrate/glucose consumption ratio of 37%-53%. Efficient utilization of butyrate, SO42-, amino acids, oligosaccharides, etc. in BFS II and the intracellular NADH contributed to the ABE fermentation performance improvement. The proposed strategy could be considered as the second utilization of waste Pichia pastoris, which could save raw materials/operating costs, fully use the oligosaccharides/SO42- in BFS II to relieve the working loads in downstream waste water treatment process, and increase fermentation products diversity/flexibility to deal with the varied marketing prices and requirements.

Anaerobically Digesting Hazardous Waste Pichia pastoris Associated with Butyric Acid Cleaner Production.

Recombinant Pichia pastoris semisolid hazardous waste treatment is difficult and traditional solid waste treatment is not applicable. However, P. pastoris wastes have features of high density and enriched proteins/polysaccharides, which could supply nitrogen/carbon sources for butyric acid production. The waste P. pastoris was first treated using NaOH to form a waste yeast suspension, and then the suspension was mixed with glucose to obtain a starting medium containing 5.6 g DCW/L (dry cell weight) yeast to initiate butyrate fermentation. The suspension was intermediately supplemented to bring the total waste yeast concentration to 26.3 g DCW/L while continuously feeding the concentrated glucose solution. With the proposed strategy, butyrate concentration reached high levels of 51.0-54.0 g/L using Clostridium tyrobutyricum as the strain. Amino acids/oligosaccharides/SO4 2- in the suspension, raw material costs, complicated pretreatment process, and butyric acid cleaner production could be effectively utilized, reduced, eliminated, and realized. However, the apparent waste P. pastoris reduction rate was only 49% per batch, thus a "tanks in-series type's repeated waste treating system" model was developed to theoretically explore the possibility of increasing the waste yeast reduction rate R. The simulation results indicated that when setting the treatment unit numbers at 4, waste solid concentration could decrease from 26.3 to 3.37 g DCW/L and the hazardous waste yeast reduction rate R would increase from 49 to 97%.

Epitaxial growth of large-grain-size ferromagnetic monolayer CrI3 for valley Zeeman splitting enhancement.

Two-dimensional (2D) magnetic CrI3 has received considerable research attention because of its intrinsic features, including insulation, Ising ferromagnetism, and stacking-order-dependent magnetism, as well as potential in spintronic applications. However, the current strategy for the production of ambient-unstable CrI3 thin layer is limited to mechanical exfoliation, which normally suffers from uncontrollable layer thickness, small size, and low yet unpredictable yield. Here, via a confined vapor epitaxy (CVE) method, we demonstrate the mass production of flower-like CrI3 monolayers on mica. Interestingly, we discovered the crucial role of K ions on the mica surface in determining the morphology of monolayer CrI3, reacting with precursors to form a KIx buffer layer. Meanwhile, the transport agent affects the thickness and size of the as-grown CrI3. Moreover, the Curie temperature of CrI3 is greatly affected by the interaction between CrI3 and the substrate. The monolayer CrI3 on mica could act as a magnetic substrate for valley Zeeman splitting enhancement of WSe2. We reckon our work represents a major advancement in the mass production of monolayer 2D CrI3 and anticipate that our growth strategy may be extended to other transition metal halides.

[Carbon storage of Pinus thunbergii and Robinia pseudoacacia plantations on Nanchangshan Island, Changdao County of Shandong Province, China].

Using indigenous tree species to transform large area pure plantations has been an effective close-to-nature forest management mode in China islands. By using the biomass allometric equation and combined with the investigation data from sampling plots, the carbon storage of the dominant species Pinus thunbergii and Robinia pseudoacacia in the plantations on the Nanchangshan Island of Miaodao Archipelago in Changdao County of Shandong Province was estimated. The average carbon storage in the arbor layer of P. thunbergii and R. pseudoacacia plantations was 56.81 and 37.26 t x hm(-2), respectively, being higher than the average carbon stock (27.62 t x hm(-2)) of tree plantations in Shandong Province. Slope aspect and stand density were the important environmental and biological factors affecting the tree's carbon stock in the Island, respectively. There was no significant correlation between the average cumulative rate of biomass in the arbor layer of P. thunbergi plantation and the stand age. The P. thunbergii with good carbon sequestration function could be an ideal tree species on Nanchangshan Island.

Characteristics of penicillin bacterial residue.

IMPLICATIONS: During the production of penicillin, a mass of waste bacterial residue is generated. In the past, the bacterial residues have been used for food additives. Unfortunately, doubts of their suitability as a feedstock have been raised because of the small amount of antibiotics and the degradation products remaining in the bacterial residues. So, penicillin bacterial residue is one of the hazardous wastes. Therefore, penicillin bacterial residue should be managed in accordance with the hazardous waste. To get a right method, the penicillin bacterial residue was characterized.