1.
STAR Protoc
; 4(3): 102391, 2023 Sep 15.
Artículo
en Inglés
| MEDLINE
| ID: mdl-37405925
RESUMEN
Here, we present a protocol for isolating human hepatocytes and neural progenitor cells from normal and nonalcoholic steatohepatitis livers. We describe steps for perfusion for scaled-up liver cell isolation and optimization of chemical digestion to achieve maximal yield and cell viability. We then detail a liver cell cryopreservation and potential applications, such as the use of human liver cells as a tool to link experimental and translational research.