COMPARATIVE CHARACTERISTICS OF SPERMOGRAPHIC INDICATORS IN IDIOPATHIC FORMS OF INFERTILITY IN MEN FROM RADIOACTIVELY CONTAMINATED AND CONVENTIONALLY CLEAN REGIONS OF UKRAINE. / PORIVNIaL'NA KhARAKTERYSTYKA POKAZNYKIV SPERMOGRAMY PRY IDIOPATYChNYKh FORMAKh BEZPLIDDIa U ChOLOVIKIV Z RADIATsIY̆NO ZABRUDNENYKh TA UMOVNO ChYSTYKh REGIONIV UKRAÏNY.

OBJECTIVE: the compare of men reproductive function with idiopathic infertility on the grounds of spermogram tests from the radioactively contaminated and conventionally clean regions of Ukraine. MATERIALS AND METHODS: There are 143 men (group I) with idiopathic infertility in the form of asthenozoospermia of the II degree living in the territories of the Kyiv region were investigated in the Department of Sexual Pathology and Andrology of the SI «Institute of Urology of the National Academy of Medical Sciences of Ukraine¼ and exposed to ionizing radiation due to the Chornobyl accident in 1986. All individuals in the I group were born from parents who were in the accident at the Chornobyl Nuclear Power Plant in the area of radioactive contamination and perma- nently resided in areas with a high level of radioactivity. The control group was made up of persons born and living in the Ivano-Frankivsk region with a similar with a similar state of health (group II, n = 157). The mean age of the studied group I was (28.3 ± 2.4) years, and persons of the II group were (30.1 ± 2.8) years. Diagnosis of II was per- formed ambulatory in accordance with WHO and Ministry of Health of Ukraine standards. The research was to study and compare spermogram parameters in patients from both groups. RESULTS: It has been established that the reproductive problems of men with idiopathic infertility living in radia- tively contaminated areas of the Kyiv region of Ukraine consist in a significant violation of the quality indicators of ejaculate (mobility by the pool A+B is less than 1.6 times, р < 0.05) correlating with modifications of physicochemi- cals (the average time of liquefaction is 1.4 times greater, р < 0.05) and biochemical (decrease of levels of fructose by 2.2 times, citrate - by 1.6 times, р < 0.001-0.05) investigation. CONCLUSIONS: The study found that the level of general indicators of spermogram is significantly worse in the regions of Ukraine who were exposed to radioactive contamination due to the Chornobyl accident, in contrast to the indicators of persons from conventionally clean regions.

Phospholipid composition of human sperm and seminal plasma in relation to sperm fertility.

The phospholipid and fatty acid composition of sperm was studied in 8 healthy and 16 infertile men. Infertile men randomly formed from the patients with normal semen parameters according to WHO criterion. Therefore, all semen parameters of infertile patients were similar to the same characteristics of the semen of healthy men, except the abnormal forms. The amount of abnormal forms in infertile men was significantly higher than in healthy men. Sperm from infertile men show a drastic loss of phosphatidyl ethanolamine. At the same time, the significant increase of phosphatidyl serine in the sperm and seminal plasma of sterile patients was found. Lysophosphatidyl serine in the sperm of the infertile men was detected. Fatty acid composition of the semen of infertile men was altered. The levels of stearic and n-3 polyunsaturated fatty acids (eicosopentaenoic and docosahexaenoic acids) was dramatically lowered, but the values of some n-6 polyunsaturated fatty acids (linolenic and docosatetraenoic) acids increased. There was significant positive correlation between docosahexaenoic acid and sperm motility (r = .82, p < .001) and negative correlation between linolenic acid and spermatozoa motility (r = -0.58. p < .05). Infertility of men with normal semen quality can originate from the disorder of sperm lipid metabolism. The drastic loss of phosphatidyl ethanolamine and n-3 polyunsaturated fatty acids with simultaneous enhancement of phosphatidyl serine and some n-6 polyunsaturated fatty acids in sperm could be an important cause of male infertility.