Evaluating the Impact of Oleocanthal and Oleacein on Skin Aging: Results of a Randomized Clinical Study.

The prevalence of skin aging and the request for effective treatments have driven dermatological research towards natural solutions. This study investigates the anti-aging efficacy of two bioactive natural polyphenols, Oleocanthal and Oleacein, in a skincare formulation. A single-blind, randomized clinical trial involved 70 participants, using a comprehensive exclusion criterion to ensure participant safety and study integrity. Participants applied the Oleocanthal and Oleacein 1% serum formulation twice daily for 30 days. The efficacy was objectively assessed using the VISIA® Skin Analysis System at baseline, after 15 days, and after 30 days. Results indicated significant wrinkle reduction in most groups. For women aged 45-79 years, the mean change was -33.91% (95% CI: -46.75% to -21.07%). For men aged 20-44 years, it was -51.93% (95% CI: -76.54% to -27.33%), and for men aged 45-79 years, it was -46.56% (95% CI: -58.32% to -34.81%). For women aged 20-44 years, the change was -25.68% (95% CI: -63.91% to 12.54%), not statistically significant. These findings highlight the potential of EVOO-derived polyphenols in anti-aging skincare, particularly for older adults. This research paves the way for further exploration into natural compounds in dermatology, particularly for aging skin management.

Fresh vs. frozen embryo transfer in assisted reproductive techniques: a single center retrospective cohort study and ethical-legal implications.

OBJECTIVE: Several studies have shown higher pregnancy rates and better perinatal outcomes with frozen embryo transfers than with fresh techniques, with better results in patients with polycystic ovary syndrome (PCOS) but with a higher rate of pregnancy complications such as preeclampsia. This retrospective cohort study aims to compare the cumulative live birth rates, maternal and neonatal complications of fresh embryo transfers (ET) and frozen-embryo transfers (FET) in infertile women who underwent assisted reproduction techniques (ART) at the Azienda Ospedaliera Ospedali Riuniti (AOOR) Villa Sofia Cervello, Palermo, Italy. In addition, the authors have focused on the legislative and ethical complexities which such a procedure entails. PATIENTS AND METHODS: Out of 475 women undergoing in vitro fertilization programs from January 2017 to January 2021, 128 were enrolled; 70 patients underwent ET, and 58 patients FET. The main outcome measure was live birth rates. Secondary outcomes were clinical pregnancy, ongoing pregnancy, pregnancy loss, low birth weight (LBW), ectopic pregnancy, and obstetrical and perinatal complications. RESULTS: The cumulative live birth rates were similar between the fresh transfer (95.7%) and frozen transfer (93.1%). Biochemical pregnancy rates, clinical pregnancy, ongoing pregnancy, and pregnancy loss were similar between the groups. CONCLUSIONS: Obstetrical outcomes were not statistically different between the two groups; a higher preterm delivery rate was reported in the FET group. ET birth weights were notably lower for singletons compared to the freeze-all strategy. ET patients also had higher LBW rates, with a 2.5-fold higher rate compared to FET. No significant differences were found in cumulative live birth rates between ET and FET, which is consistent with earlier studies. FET protocols are linked to higher neonatal birth weight and lower risk of LBW than fresh ET. The ethical and legal quandaries inherent in such techniques, as technology moves on and outpaces current legislative frameworks, cannot be discounted.

Update on fertility preservation: new opportunities and challenges in the fight against infertility.

Abstract: Infertility has been characterized as a disease by the World Health Organization (WHO) and reportedly affects about 10-12% of couples worldwide, while the incidence is even higher in Italy, at about 15%. The issue of iatrogenic infertility arising from treatments that can compromise an individual's reproductive capacity, it is necessary to inform patients of the possible damage on their future fertility and on the possibilities to preserve it. The complexities inherent in the various techniques and approaches aimed at preserving fertility should be expounded upon thoroughly to the patients, who should also receive proper psychological assistance and counseling, which ought to take into account the ethical distinctive challenges and the possible misgivings that may be caused in patients. Ovarian Tissue Cryopreservation (OTC) and ovarian tissue transplantation (OTT) can constitute a valuable part of the clinical armamentarium for preserving fertility, although the data are still inconclusive, particularly in over-36 patients. The multidisciplinary nature of the healthcare teams involved in such interventions is of paramount importance to optimize results.

Gender differences in adverse outcomes after blunt trauma.

BACKGROUND: High testosterone and low estradiol levels induce immunosuppression and adverse outcome after trauma in male animals. Gender-based outcome differences in human trauma have not been investigated. In order to test our hypothesis that female gender is associated with improved outcome after trauma, we conducted an inception cohort study at the R. Adams Cowley Shock Trauma Center, the adult trauma resource center for the state of Maryland. METHODS: All were blunt trauma patients (18,892) admitted from 1983 to 1995, stratified by Injury Severity Score (ISS) and age. Gender differences in mortality; nosocomial infection; and preinjury diabetes and cardiac, pulmonary, and liver diseases were determined. RESULTS: No significant differences in preinjury diseases were identified. Death and gender were independent variables in all groups except for patients who developed pneumonia. Male patients had a higher incidence of pneumonia in all groups except age 18 to 45, with an ISS < 15. The association between male gender and pneumonia was strongest in the age 46 to 65, ISS > 30 subgroup (p < 0.01). Among those with pneumonia, female patients were at 2.8 to 5.6 times higher risk for death than were male patients. CONCLUSION: These data suggest that gender has no relation to mortality in blunt trauma patients who do not develop pneumonia. In contrast, male gender was significantly associated with an increased incidence of pneumonia after injury, and female patients with pneumonia were at significantly higher risk for mortality.

Social cognitive predictors of nutritional risk in rural elderly adults.

According to Social Cognitive Theory (Bandura, 1997), nutritional risk would be predicted by perceptions of nutrition efficacy, which in turn would be predicted from four sources: modeling, verbal support, physiological conditions, and nutrition habits. In telephone interviews with 154 rural elderly adults (44 men, 110 women; mean age = 74.4 years), nutritional risk was measured with Nutritional Risk Index (NRI), Nutritional Screening Initiative (NSI), and seven-high-risk-nutrients consumed less than 50 percent of RDA (Diet Plus Analysis). Nutrition-efficacy was measured with a new twenty-five-item scale of Perceived Nutrition Barriers (PNB). Sources of nutrition-efficacy included: mealtime modeling--shared meals, household size; verbal support--people talk to each day, hours talked, number of confidants; physiological conditions that may affect nutrition--age, body mass, medications, disability, negative affect, and nutrition habits--daily food variety, use of meal services. Path analysis was performed with each measure of nutritional risk (NRI, NSI, 7-high-risk-nutrients) as a criterion variable, nutrition-efficacy (PNB) as a possible mediating variable, and sources of nutrition-efficacy as predictor variables. Social Cognitive variables accounted for 58 percent of variance in NRI, 49 percent of variance in NSI, and 29 percent of variance in seven-high-risk-nutrients. Nutritional risk was directly predicted by large households, few shared meals, few confidants, high body mass, many medications, and few daily foods; it was indirectly predicted (via PNB) by high levels of negative affect. Perceived Nutrition Barriers (PNB) most often mentioned were food cost, eating alone, food tastelessness, transportation to the store, and chewing difficulty.

The brain-to-liver mercury ratio increases with aging in mice.

There was no significant change in the total amount of mercury in organs (lung, heart, kidney, brain, and liver) from male C57BL/6J mice ranging in age from 133 to 904 days of age maintained under conventional conditions with no known source of mercury exposure other than background concentrations. The lowest values were found in the liver and the highest in the brain, with considerable variation in the mercury content between individual mice for all organs examined. The ratio of mercury in the brain to that in the liver, however, was found to significantly increase with aging in an exponential manner. A similar result was found for the ratio of brain to kidney mercury. We conclude that older mice are less able to maintain low brain-to-liver ratios of mercury regardless of the total body content of mercury. Dietary mercury ranging from 200 to 20,000 ppm Hg had little or no influence on the life span of Drosophila fruit flies, suggesting that the effect of mercury is probably not on life span itself but on other factors associated with the aging process such as neurological disfunction.