[Proteomic methods of protein separation and analysis].

The review briefly presents principles and stages of execution of such highly effective methods of protein separation as capillary, 2D gel-electrophoresis, liquid chromatography, the possibility of their successful combination with tandem mass-spectrometry and application in various fields of proteomics. The main problems of proteomic analysis as well as ways of solving by using mass-spectrometry are examined. 2 main approaches during protein identification are described in the review, characteristics and possibilities of various top-down and bottom-up proteomic analytical programs are provided.

[Mass spectrometry in medicine and biotechnology].

History of development and improvement of tandem mass spectrometry, possibilities of its application at the contemporary stage in various fields of medicine and biotechnology including production of novel medicinal preparations, identification of biologically active substances, pathogenic microorganisms and causative agents of especially dangerous infections is given.

[Role of burrow microbiocenosis in plague enzootia].

The paper analyzes relationships of the plague bacilli to the representatives of different types of living organisms inhabiting the burrows. The authors give their own data on the qualitative and quantitative composition of indicoles of the burrow of little sousliks (Spermophillus pygmaeus). They assess the role of mutagenic agents in burrow microbiocenoses.

[Serum lipids in pulmonary tuberculosis patients with various types of diabetes mellitus]. / Lipidy syvorotki krovi u bol'nykh tuberkulezom legkikh s raznymi tipami sakharnogo diabeta.

In 12 patients with pulmonary tuberculosis and in 14 with the disease concurrent with types I and II diabetes mellitus, a somatotype was determined and lipid metabolism was studied by the blood levels of various lipid fractions, by the fractional composition of lipoproteins and atherogenicity coefficient. Lipid metabolic changes in patients with tuberculosis and in those with the accompanied abnormality were not found to depend upon sex, age, somatotype and the type of tuberculosis, but are apparently related with the severity of tuberculous intoxication. In the concurrent disease, lipid metabolic changes depended on the type of diabetes.