RESUMEN
Stimforte in a wide range of concentrations (15-225 µg/mL) totally inhibits the cytopathic activity of hepatitis C virus (HCV) in the Vero-V cell culture. Interferons (IFN) play the most important role in the suppression of infection when the drug is introduced into the culture before the infection. When Stimforte is introduced after the infection, the mechanism of action seems to be different. The activators of IFN production are mainly (or exclusively) the ligands of receptor complexes TLR-4 and NOD-2 contained in the drug. The action of these substances is probably synergistic, similar to the action of LPS and MDP in Vero-V cells.
Asunto(s)
Antivirales/farmacología , Hepatitis C/tratamiento farmacológico , Compuestos Orgánicos/farmacología , Acetilmuramil-Alanil-Isoglutamina/farmacología , Adyuvantes Inmunológicos/administración & dosificación , Adyuvantes Inmunológicos/farmacología , Animales , Antivirales/administración & dosificación , Supervivencia Celular/efectos de los fármacos , Chlorocebus aethiops , Relación Dosis-Respuesta a Droga , Hepacivirus/efectos de los fármacos , Hepacivirus/fisiología , Hepatitis C/inmunología , Interferones/metabolismo , Lipopolisacáridos/administración & dosificación , Lipopolisacáridos/farmacología , Proteína Adaptadora de Señalización NOD2/metabolismo , Compuestos Orgánicos/administración & dosificación , Receptor Toll-Like 4/metabolismo , Células Vero , Replicación Viral/efectos de los fármacosRESUMEN
The induction profile of endogenous interferons of three types under the action of meglumine acridone acetate and sodium acridone acetate, as well as the characteristics of the kinetics of their accumulation and circulation in the blood of experimental animals are described. Meglumine acridone acetate provided balance in induction, production and circulation of the interferons in the blood vs. sodium aeridone acetate under the same conditions. The optimal dose of meglumine acridone acetate (2.0-3.5 mg/mouse) with effective, consecutive and overlapping induction-production of the interferons ensured their prolonged (for 72 hours) circulation in the blood after a single administration.
Asunto(s)
Acridonas/administración & dosificación , Citocinas/biosíntesis , Inductores de Interferón/administración & dosificación , Interferón-alfa/biosíntesis , Interferón gamma/biosíntesis , Meglumina/administración & dosificación , Animales , Citocinas/sangre , Femenino , Interferón-alfa/sangre , Interferón gamma/sangre , Ratones , Ratones Endogámicos CBA , Factores de TiempoRESUMEN
Acute experiments were performed on rabbits to study the responses of neurons in the anterior, ventromedial, and posterior nuclei of the hypothalamus to single, paired, and rhythmic stimulation of the vestibular nerve and lateral vestibular nucleus of Deiters. The data obtained showed that neurons of the posterior nucleus of the hypothalamus were the most sensitive. Three types of response were seen from hypothalamic neurons, with short, long, and intermediate latent periods. This provides evidence that ascending afferent spike activity from the lateral vestibular nucleus of Deiters to the hypothalamus is mediated by mono-, oligo-, and polysynaptic pathways.
