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1.
Elife ; 82019 04 29.
Artículo en Inglés | MEDLINE | ID: mdl-31033442

RESUMEN

After Salmonella is phagocytosed, it resides in an acidic vacuole. Its cytoplasm acidifies to pH 5.6; acidification activates pathogenicity island 2 (SPI-2). SPI-2 encodes a type three secretion system whose effectors modify the vacuole, driving endosomal tubulation. Using super-resolution imaging in single bacterial cells, we show that low pH induces expression of the SPI-2 SsrA/B signaling system. Single particle tracking, atomic force microscopy, and single molecule unzipping assays identified pH-dependent stimulation of DNA binding by SsrB. A so-called phosphomimetic form (D56E) was unable to bind to DNA in live cells. Acid-dependent DNA binding was not intrinsic to regulators, as PhoP and OmpR binding was not pH-sensitive. The low level of SPI-2 injectisomes observed in single cells is not due to fluctuating SsrB levels. This work highlights the surprising role that acid pH plays in virulence and intracellular lifestyles of Salmonella; modifying acid survival pathways represents a target for inhibiting Salmonella.


Asunto(s)
Ácidos/farmacología , Proteínas Bacterianas/metabolismo , Concentración de Iones de Hidrógeno , Proteínas de la Membrana/metabolismo , Conformación Molecular/efectos de los fármacos , Salmonella typhimurium/efectos de los fármacos , Salmonella typhimurium/metabolismo , Factores de Transcripción/metabolismo , Proteínas Bacterianas/efectos de los fármacos , Citoplasma , Proteínas de Unión al ADN/efectos de los fármacos , Proteínas de Unión al ADN/metabolismo , Regulación Bacteriana de la Expresión Génica , Histidina Quinasa/metabolismo , Proteínas de la Membrana/efectos de los fármacos , Regiones Promotoras Genéticas , Salmonella typhimurium/citología , Salmonella typhimurium/genética , Transactivadores/metabolismo , Factores de Transcripción/efectos de los fármacos , Vacuolas/metabolismo , Virulencia
2.
Nucleic Acids Res ; 46(19): 10216-10224, 2018 11 02.
Artículo en Inglés | MEDLINE | ID: mdl-30239908

RESUMEN

Heat-stable nucleoid structuring protein (H-NS) plays a crucial role in gene silencing within prokaryotic cells and is important in pathogenesis. It was reported that H-NS silences nearly 5% of the genome, yet the molecular mechanism of silencing is not well understood. Here, we employed a highly-sensitive single-molecule counting approach, and measured the dissociation constant (KD) of H-NS binding to single DNA binding sites. Charged residues in the linker domain of H-NS provided the most significant contribution to DNA binding affinity. Although H-NS was reported to prefer A/T-rich DNA (a feature of pathogenicity islands) over G/C-rich DNA, the dissociation constants obtained from such sites were nearly identical. Using a hairpin unzipping assay, we were able to uncouple non-specific DNA binding steps from nucleation site binding and subsequent polymerization. We propose a model in which H-NS initially engages with non-specific DNA via reasonably high affinity (∼60 nM KD) electrostatic interactions with basic residues in the linker domain. This initial contact enables H-NS to search along the DNA for specific nucleation sites that drive subsequent polymerization and gene silencing.


Asunto(s)
Proteínas Bacterianas/metabolismo , Proteínas de Unión al ADN/metabolismo , ADN/metabolismo , Proteínas Bacterianas/química , Sitios de Unión/genética , ADN/química , ADN Bacteriano/metabolismo , Proteínas de Unión al ADN/química , Regulación Bacteriana de la Expresión Génica , Silenciador del Gen , Islas Genómicas/genética , Unión Proteica , Imagen Individual de Molécula , Especificidad por Sustrato
3.
J Biol Chem ; 289(20): 13739-50, 2014 May 16.
Artículo en Inglés | MEDLINE | ID: mdl-24668810

RESUMEN

The locus of enterocyte effacement-encoded regulator (Ler) of enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC) functions to activate transcription of virulence genes silenced by the histone-like nucleoid-structuring protein (H-NS). Despite its important role in the bacterial gene regulation, the binding mode of Ler to DNA and its mechanism in alleviating genes repressed by H-NS are largely unknown. In this study, we use magnetic tweezers to demonstrate that Ler binds extended DNA through a largely noncooperative process, which results in DNA stiffening and DNA folding depending on protein concentration. We also show that Ler can replace prebound H-NS on DNA over a range of potassium and magnesium concentrations. Our findings reveal the DNA binding properties of Ler and shed light to further understand the anti-silencing activity of Ler.


Asunto(s)
Proteínas Bacterianas/metabolismo , Unión Competitiva , ADN Bacteriano/metabolismo , Proteínas de Unión al ADN/metabolismo , Escherichia coli Enterohemorrágica/metabolismo , Escherichia coli Enteropatógena/metabolismo , Proteínas de Escherichia coli/metabolismo , Transactivadores/metabolismo , ADN Bacteriano/química , Relación Dosis-Respuesta a Droga , Escherichia coli Enterohemorrágica/genética , Escherichia coli Enteropatógena/genética , Concentración de Iones de Hidrógeno , Cloruro de Magnesio/farmacología , Conformación de Ácido Nucleico , Cloruro de Potasio/farmacología , Unión Proteica/efectos de los fármacos , Especificidad por Sustrato , Temperatura
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