Dynamic changes of microbial community and moisture ratio during bio-drying of sludge after electro-dewatering.

Using electro-dewatering as the pretreatment process for sludge bio-drying can improve the dewatering performance. It was innovatively investigated including the microbial mechanism and the kinetics of moisture removal by bio-drying with electro-dewatered sludge in this study. Two bio-drying processes using electro-dewatered sludge (EDS) and sludge added cornstalk conditioner (CSS) were compared. Microbial community analysis showed that the abundance of Bacteroidetes increased from 4.21% to 16.67% after electro-dewatering. The dominant phyla were Bacteroidetes (36.79%), Proteobacteria (32.35%), and Actinobacteria (24.58%) at the end of EDS bio-drying. Network analysis revealed that the co-occurrence patterns in EDS included 40 nodes and 97 edges. The prediction results of the Kyoto Encyclopedia of Genes and Genomes demonstrated that the relative abundances of carbohydrate metabolism and metabolism of terpenoids and polyketides in sludge decreased, while the relative abundances of lipid metabolism, xenobiotic biodegradation and metabolism increased after electro-dewatering. Five thin layer drying kinetic models were analyzed to estimate the bio-drying kinetic parameters. The Page's model could be better fitted to the results and the highest R2 was 0.9570 in the EDS. The new coefficients k (0.1637) and n (1.2097) were obtained. The results provided mechanism and data support for exploring and applying bio-drying technology after sludge electro-dewatering.

Semi-solid state promotes the methane production during anaerobic co-digestion of chicken manure with corn straw comparison to wet and high-solid state.

Total solid content (TS) is an important factor for biogas production during anaerobic digestion. In this study, we explored the influence of different TS (5% wet, 15% semi-solid and 25% solid state) on the relative cumulative methane production (RCMP) during anaerobic co-digestion of chicken manure with corn straw. Results showed that total ammonium nitrogen and free ammonia nitrogen concentration increased with the increase of TS. Ammonium nitrogen in treatments at 15% TS was 2.25-2.76 times as high as that at 5% TS, which was below 3 times. The highest chemical oxygen demand removal and RCMP were obtained in the treatment of 15% TS with a ratio of 2:1 chicken manure: corn straw (based on TS). The RCMP in the treatments of 15% TS were 3.63-4.59 times higher than that of 5% TS based on the volume of substrates. The abundance of Caldicoprobacter improving the degradation of corn straw was significantly positively correlated with the RCMP, and the average abundance of Caldicoprobacter at 15% TS was 8.33 and 7.02 times higher than that at 5% and 25% TS, respectively. Structural equation models analysis suggested that TS significantly impacted the RCMP by indirectly impacting free ammonia nitrogen and microbial abundance. These findings indicated semi-solid state (15% TS) decreased ammonia nitrogen releasing and improved the abundance of Caldicoprobacter, and increased RCMP during anaerobic co-digestion of chicken manure with corn straw.

Fate of antibiotic resistance genes during high solid anaerobic digestion with pig manure: Focused on different starting modes.

As an emerging technology, high solid anaerobic digestion (HSAD) was usually hampered by the long lag phase of methane production. A reasonable starting mode enabled fast startup in HSAD, which was scarcely reported. This study established 5 starting modes for HSAD with pig manure. The results showed that system T4 (biogas slurry once and then autologous leachate reflux) had the shortest lag phase. Starting modes had a total effect of 36.6% on gas production, among which 17.1% affected gas production directly and 19.5% affected it through other factors. About 12/17 of antibiotic resistance genes (ARGs) and 3 mobile genetic elements (MGEs) were effectively reduced during HSAD. System T4 had the highest microbial diversity and the largest number of unique OTUs. MGEs explained most for ARGs variation (>50%), followed by microbial community. Most of the potential host genera for ARGs belonged to Firmicutes phyla, which could be decreased by starting modes.

Lignite as additives accelerates the removal of antibiotic resistance genes during poultry litter composting.

Antibiotic resistance genes (ARGs) in animal manure are a great threat to human health. This study investigated the effects of lignite addition at three levels (5%, 10%, 15% w/w) on the profiles of ARGs and the bacterial communities during poultry litter composting. Lignite addition effectively promoted the removal of manure-borne ARGs. After 65 days of composting, the relative abundances of ARGs decreased by 8.9% in control (no lignite), and by 15.8%, 27.7% and 41.5% in 5%, 10% and 15% lignite treatments, respectively. Although the total mobile genetic elements were enriched after composting, the enrichment of the intI-1 gene was significantly lower in the 10% and 15% lignite treatments compared with control. Network analysis indicated that Actinobacteria and Firmicutes were potential bacterial hosts for ARGs. Redundancy analysis showed that bacterial community succession played a key role in the shifts of ARGs. Taken together, this study provides evidence that lignite as additives promoted the removal efficacy of ARGs during composting of poultry litter.

Fate of antibiotic resistance genes during high-solid anaerobic co-digestion of pig manure with lignite.

Lignite could be used to promote methane production during high-solid anaerobic co-digestion (HS-AcoD) of pig manure, however, the effects of lignite amendment on the fate of ARGs during HS-AcoD are unknown. Here, we explored the influence of lignite (0%, 8%, 16%, 32%, and 64%) on the fate of ARGs during HS-AcoD of pig manure. The results showed that 16% lignite reduced the absolute abundance of ARGs by 28.71% compared with the 0% lignite treatment. Variation partitioning analysis suggested the combined effect of microbial community, mobile genetic elements (MGEs) and environmental factors was the major driver shaping the pattern of ARGs. The potential hosts of ARGs were Bifidobacterium, Lactobacillus, Tissierella and Streptococcus. Structural equation models analysis suggested lignite indirectly impacted the pattern of ARGs by significantly reducing the abundance of microbial community and MGEs. These findings give an insight into the mechanistic understanding of the lignite influence on the reduction of ARGs during HS-AcoD.

Biodegradation of volatile solids and water mass balance of bio-drying sewage sludge after electro-dewatering pretreatment.

Using pressurized electro-osmotic dewatering (PEOD) as the pretreatment process for sewage sludge (SS) bio-drying can improve the dewatering performance, but the kinetics of volatile solids biodegradation and the water mass balance are still unknown. These processes were first investigated in this study. Experiments were conducted with three different initial materials, which were composed of SS, bio-dried product and SS dewatered by PEOD (EDSS) as different mass ratios. Six kinetic models and a nonlinear regression method were used to estimate the kinetic parameters, and the models were analyzed using four statistical indicators. Satisfactory fitting of the proposed kinetic model to the experimental data was achieved. Through the water mass balance, the results showed that EDSS had the best dewatering performance for bio-drying. EDSS provided the most appropriate conditions for the bio-drying process; the highest correlation coefficient was 0.9291 and the total water removal rate was 51.13% in the bio-drying of all EDSS.

Pressurized electro-dewatering of activated sludge: Analysis of electrode configurations (anode).

An electric field and mechanical pressure combined are considered an effective electro-dewatering (PED) technology for activated sludge. Here, the curved surface anodes were used for electro-dewatering to improve the effective anode area, and the PED characteristics were investigated for three anode types (flat plate, sawtooth plate and wave plate). First, evaluation methodology of the modified energy consumption ( [Formula: see text] ) and the modified processing capacity ( [Formula: see text] ) were established, with electro-dewatering factor (ξED), to evaluate the PED efficiency of different anode configurations under three raw sludge processing capacity modes. Second, the solid content distribution was analyzed by the layered method, and the electric field and current density distribution were analyzed by the finite element method. Finally, the gas emission mechanism of the curved surface anodes was discussed. When the raw sludge processing capacity and dewatering time (10 min) were the same, nearly the same extent of dewatering was achieved for the wave plate anode as for the flat plate anode. The total filtrate amount was 69.5 g and 59.0 g for the wave plate and flat plate anodes, respectively, and [Formula: see text] increased by 17.8% for the former. Under the same raw sludge thickness, the dewatering percentages in area A of the sawtooth plate and wave plate anodes were 10% and 11%, respectively, higher than that of the flat plate anode. However, according to numeric simulation results, the current density at the tips of the sawtooth plate anode can reach 740-770 A/m2, which can reduce its service life as compared to flat plate anode. In area D, gas was more easily emitted from the wave plate anode than from the flat plate anode, reducing the influence of the gas barrier layer on the electrical contact between the sludge cake and the anode.

Sewage sludge drying method combining pressurized electro-osmotic dewatering with subsequent bio-drying.

In this study, pressurized electro-osmotic dewatering (PEOD) as a pretreatment process, instead of the conventional practice of adding bulking agents, for sewage sludge bio-drying was proposed. Initially, various parameters were optimized for obtaining dewatered sewage sludge (DSS), treated by an efficient, quick, and energy-saving PEOD process. The results show that the moisture content (MC) of sewage sludge could decrease from 83.41% to 60.0% within 7.5 min in the optimum conditions of the PEOD process. Subsequently, two DSS bio-drying tests were carried out to investigate the effects of inoculation. The highest temperature (68.1 °C) was obtained for T2 (inoculation), which was 3.6 °C higher than that for T1 (non- inoculation). The MC accumulative removal rate for T1 (41.49%) was slightly less than that for T2 (44.60%). Lastly, the volatile solid degradation dynamics model parameters were measured. The degradation rate constants (k) for T1 and T2 were 0.00501 and 0.00498, respectively.

[Effects of irrigation using dairy effluent on grain yield, phosphorus utilization and distribu- tion in soil profile in winter wheat-summer maize rotation system].

Field experiments of winter wheat-summer maize rotation were conducted in North China Plain irrigation area to explore the effects of wheat season irrigation with dairy effluent on grain yield, phosphorus uptake, accumulative phosphorus usage efficiency and phosphorus accumulation in soil. The results showed that the irrigation with dairy effluent significantly improved the yields of winter wheat and summer maize. With the increasing of P2O5 carried by dairy effluent into soil, winter wheat yield increased at first and then decreased. When the P2O5 increased 137 kg · hm(-2), winter wheat yield increased to the maximum (7646.4 kg · hm(-2)) and the phosphorus utilization rate was the highest (24.8%). But excessive phosphorus decreased the winter wheat yield and phosphorus utilization efficiency. Summer maize yield and phosphorus uptake increased with the increase of P2O5 carried by dairy effluent. The summer maize yield increased by 2222.4-2628.6 kg · hm(-2) and the phosphorus uptake increased by 13.9-21.1 kg · hm(-2) in contrast to the control (CK). Under conventional phosphorus fertilization at 88 kg · hm(-2), and the summer maize yield increased by 2235.0 kg · hm(-2) compared with CK. As the time of irrigation with dairy effluent increasing, the grain yield increased more significantly. The cumulative phosphorus utilization in this rotation system increased year by year. After six seasons of crop harvest, the cumulative phosphorus utilization rate increased into 40.0%-47.7%. Under the experimental condition, two times of irrigation with the dairy effluents in the winter wheat-summer maize rotation system was the best operating mode.

Influence of NaOH and thermal pretreatment on dewatered activated sludge solubilisation and subsequent anaerobic digestion: Focused on high-solid state.

In this study, the influence of NaOH and thermal pretreatment of dewatered activated sludge (DAS) on the high-solid solubilisation and anaerobic digestion was separately investigated by monitoring common parameters. The results indicated that COD, proteins and carbohydrates were efficiently solubilised in both NaOH and thermal pretreated DAS samples. For NaOH pretreatment, the concentrations of volatile fatty acids (VFAs) and total ammonium nitrogen (TAN) firstly increased followed by decreasing with NaOH dose increasing. However, they decreased with the severity of thermal pretreatment. During the batch digestion experiments (at 37°C), for 80mg NaOHg(-1) total solid (TS) DAS pretreatment it resulted in a 6.99% decrease in cumulative methane yield (CMY) compared to untreated DAS. While for 80, 100, 120°C and 20mg NaOH pretreatment, CMY increased by 15%, 42%, 71% and 35%, respectively, in comparison to untreated DAS.

Experimental investigation on water treatment by the combined nano MgO-nanofiltration technique.

A combined system using nano MgO and nanofiltration (NF) membrane was established to purify polluted water in this experiment. The turbidity, permanganate index, UVA254, colony counts and the concentrations of NO3-, NO2-, NH4+, Fe, Mn and Mg of the effluents from each unit of this combined system were measured to investigate the pollutant removal of this system. Based on the results obtained, the combined nano MgO-NF system could efficiently remove many kinds of pollutants in this experiment, including organic matter, nitrogen species, heavy metals, suspended solids and bacteria. And the effluents could meet the standard of drinking water. Furthermore, increasing the nano MgO dosage could not elevate the removal ratio of the pollutants, but only increase the Mg content of the effluent. Thus, 0.05 g L(-1) of nano MgO may be a suitable dosage for 2,000 L of polluted water treatment. Also, the operating pressure of NF membrane had no significant effect on pollutant removal when the operating pressure of NF membrane was increased from 0.3 to 0.9 MPa.