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After the publication of the article, an interested reader drew to the authors' attention that, in the western blots shown in Fig. 5C and D, a pair of data panels were inadvertently duplicated comparing between panels (C) and (D); in addition, the cell migration data shown in Fig. 7F on p. 1852 were selected incorrectly. The authors have examined their original data, and realize that these errors arose inadvertently as a consequence of their mishandling of their data. The revised versions of Figs. 5 and 7, featuring the corrected data for the caspase-8 experiment in Fig. 5C and alternative data for the cell migration assay experiments in Fig. 7F, are shown on the next two pages. The revised data shown for these Figures do not affect the overall conclusions reported in the paper. All the authors agree to the publication of this corrigendum, and are grateful to the Editor of Oncology Reports for allowing them the opportunity to publish this. Furthermore, the authors apologize to the readership for any inconvenience caused. [Oncology Reports 40: 1843-1854, 2018; DOI: 10.3892/or.2018.6593].
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Lung cancer is the most common cause of cancerassociated mortality. MicroRNAs (miRNAs), as oncogenes or tumor suppressor genes, serve crucial roles not only in tumorigenesis, but also in tumor invasion and metastasis. Although miRNAlet7a (let7a) has been reported to suppress cell growth in multiple cancer types, the biological mechanisms of let7a in lung adenocarcinoma are yet to be fully elucidated. In the present study, the molecular roles of let7a in lung adenocarcinoma were investigated by detecting its expression in lung adenocarcinoma tissues and exploring its roles in the regulation of lung cancer cell proliferation. Let7a expression was identified to be downregulated in lung adenocarcinoma tissues compared with normal tissues. Overexpression of let7a effectively suppressed cancer cell proliferation, migration and invasion in H1299 and A549 cells. Let7a also induced cell apoptosis and cell cycle arrest. Furthermore, let7a significantly inhibited cell growth by directly regulating cyclin D1 signals. This novel regulatory mechanism of let7a in lung adenocarcinoma provides possible avenues for future targeted therapies of lung cancer.
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Adenocarcinoma/patología , Biomarcadores de Tumor/metabolismo , Proliferación Celular , Ciclina D1/metabolismo , Regulación Neoplásica de la Expresión Génica , Neoplasias Pulmonares/patología , MicroARNs/genética , Adenocarcinoma/genética , Adenocarcinoma/metabolismo , Adulto , Anciano , Apoptosis , Biomarcadores de Tumor/genética , Estudios de Casos y Controles , Puntos de Control del Ciclo Celular , Movimiento Celular , Ciclina D1/genética , Femenino , Estudios de Seguimiento , Humanos , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/metabolismo , Masculino , Persona de Mediana Edad , Invasividad Neoplásica , Pronóstico , Transducción de Señal , Tasa de Supervivencia , Células Tumorales CultivadasRESUMEN
Hashing methods have been widely used for approximate nearest neighbor search in recent years due to its computational and storage effectiveness. Most existing multimodal hashing methods try to preserve the similarity relationship based on either metric distances or semantic labels in a procrustean way, while ignoring the intra-class and inter-class variations inherent in the metric space. In this paper, we propose a novel multimodal hashing method, termed as semantic neighbor graph hashing (SNGH), which aims to preserve the fine-grained similarity metric based on the semantic graph that is constructed by jointly pursuing the semantic supervision and the local neighborhood structure. Specifically, the semantic graph is constructed to capture the local similarity structure for the image modality and the text modality, respectively. Furthermore, we define a function based on the local similarity in particular to adaptively calculate multi-level similarities by encoding the intra-class and inter-class variations. After obtaining the unified hash codes, the logistic regression with kernel trick is employed to learn view-specific hash functions independently for each modality. Extensive experiments are conducted on four widely used multimodal data sets. The experimental results demonstrate the superiority of the proposed SNGH method compared with the state-of-the-art multimodal hashing methods.
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A refractive index (RI) tunable functional materials infiltrated side-hole ring fiber (SHRF) is proposed to generate 10 LP OAM states with 6 topology numbers. On the basis of perturbation theory, the basis of the SHRF is demonstrated to be the LP modes. After a fixed propagation distance of 0.03 m, 0.009 m and 0.012 m, the phase difference between the odd and even LP11x, LP21x,y, LP31x,y modes in the SHRF accumulate to ± π/2 respectively with na ranging from 1.412 to 1.44. Correspondingly, the output states are OAM ± 1x, OAM ± 2x,y, OAM ± 3x,y with a bandwidth of 380 nm, 100 nm and 80 nm respectively. The proposed fiber is easy to be fabricated with the mature fiber drawing technology and could facilitate the realization of all fiber based OAM system.
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The renal allograft survival rates of patients with immunoglobulin A nephropathy (IgAN), and patients with or without other glomerular diseases, have yet to be fully elucidated. In this study, the clinicopathological factors associated with long-term allograft survival for the prognosis of renal allograft recipients with IgAN were examined. All patients enrolled in this study were diagnosed with IgAN following clinical and pathological examinations. Patients underwent renal graft biopsy and were hospitalized at the Fuzhou General Hospital between June, 2004 and December, 2010. Common demographic and clinical indicators were recorded in patients who had graft loss and in those who had functional renal grafts. Forty-two of the 202 biopsy specimens (20.8%) met the diagnostic criteria for IgAN and were divided into two groups, the graft loss group (n=17) and the functional graft group (n=25). Patients were followed up for 1-257 months after kidney transplantation. The mean patient age was 40.6 ± 9.3 years at the time of renal graft biopsy. Examination results indicated concomitant proteinuria and hematuria in 25 patients (59.5%) and proteinuria alone in six patients (14.3%). Graft loss occurred in 17 patients during the follow-up period. Comparison of the graft loss and the functional graft groups indicated that patients in the graft loss group were more likely to have proteinuria (P=0.047), high creatinine levels at the time of biopsy (P=0.009), low glomerular filtration rates (P=0.013), low serum total protein (P=0.01), a high Banff score (P=0.001), extensive glomerulosclerosis (P=0.002), a greater likelihood of crescent formation (P=0.01), severe tubular atrophy (P=0.013) and more extensive interstitial fibrosis (P=0.033). However, the two groups showed no significant differences in blood pressure, hematuria, BUN, UA, Hb, TG and CHO levels. The allograft survival rate of patients with IgAN was identified to be similar to that of patients with and without other glomerular diseases.
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Aloinjertos/patología , Glomerulonefritis por IGA/patología , Glomerulonefritis por IGA/terapia , Supervivencia de Injerto , Trasplante de Riñón , Adulto , Estudios de Casos y Controles , Demografía , Femenino , Glomerulonefritis por IGA/mortalidad , Humanos , Masculino , Pronóstico , Tasa de SupervivenciaRESUMEN
BACKGROUND: The objective of this study was to evaluate the effect of bone marrow mesenchymal stem cells (BMSCs) on the apoptosis of granulosa cells (GCs) in rats. RESULTS: Cisplatin increased GC apoptosis from 0.59% to 13.04% in the control and cisplatin treatment groups, respectively, which was significantly reduced upon co-culture with BMSCs to 4.84%. Cisplatin treatment increased p21 and bax and decreased c-myc mRNA expression, which was reversed upon co-culture with BMSCs. As compared to young rats, increased apoptosis was observed in the perimenopausal rats (P < 0.001). After 3 months, the apoptosis rate in the BMSC group was significantly lower than that of the control group (P = 0.007). CONCLUSIONS: BMSC therapy may protect against GC apoptosis induced by cisplatin and perimenopause. Further studies are necessary to evaluate therapeutic efficacy of BMSCs.
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Células de la Médula Ósea/efectos de los fármacos , Cisplatino/administración & dosificación , Técnicas de Cocultivo/métodos , Inhibidor p21 de las Quinasas Dependientes de la Ciclina/metabolismo , Células de la Granulosa/efectos de los fármacos , Trasplante de Células Madre Mesenquimatosas , Células Madre Mesenquimatosas/efectos de los fármacos , Perimenopausia/fisiología , Animales , Apoptosis/efectos de los fármacos , Células de la Médula Ósea/fisiología , Células Cultivadas , Inhibidor p21 de las Quinasas Dependientes de la Ciclina/genética , Estrógenos/administración & dosificación , Femenino , Regulación de la Expresión Génica/efectos de los fármacos , Células de la Granulosa/fisiología , Células Madre Mesenquimatosas/fisiología , Proteínas Proto-Oncogénicas c-myc/genética , Proteínas Proto-Oncogénicas c-myc/metabolismo , Ratas , Ratas Sprague-Dawley , Proteína X Asociada a bcl-2/genética , Proteína X Asociada a bcl-2/metabolismoRESUMEN
OBJECTIVE: To establish a formula for estimating area under the concentration-versus-time curve (AUC) of mycophenolate sodium in Chinese renal allograft recipients with a limited sampling model. METHODS: A total of 35 renal allograft recipients were recruited from 2010 to 2013 to receive enteric-coated mycophenolate sodium (EC-MPS), calcineurin (CNI) and prednisone as immunosuppressive triple therapy. The serum concentration of mycophenolic acid (MPA) was assayed by enzyme multiplied immunoassay technique (EMIT) at pre-dose (C0), 0.5 (C0.5), 1.0 (C1), 1.5 (C1.5), 2.0 (C2), 3.0 (C3), 4.0 (C4), 6.0 (C6), 8.0 (C8) and 12.0 (C12) h post-dose respectively. Pharmacokinetic parameters of MPA (C0, C12, Cmax, Tmax, AUC0-12 h) were calculated by software WINNOLIN. Simplified formulae for estimation of MPA-AUC in tacrolimus (Tac) group or cyclosporin A (CsA) group were established by multiple stepwise regression analysis. RESULTS: There were variable MPA AUC0-12 h levels between 14 and 67 mg×h/L (mean: 37 ± 14). The MPA trough level (C0) had no correlations with MPA AUC0-12 h (r(2) = 0.090) . The simplified MPA AUC formula for Tac group was AUC = 5.678+1.718×C4+2.853×C6+1.812×C8+3.413×C12 with four sampling points (C4, C6, C8, C12). Estimated AUC with the formula had correlations with AUC0-12 h (r(2) = 0.890). The mean absolute predict error (APE) was 3.45% (0.41%-24.71%) and the proportion of APE above 15% stood at 11.1% (2/18) . In CsA group, the simplified MPA AUC formula was AUC = 7.072+1.525×C3+1.558×C4+ 1.573×C6+2.285×C8. The correlation was r(2) = 0.952, mean APE was 6.50% (0.02%-12.91%) and proportion of APE above 15% stood at 0. The above formulae were observed to have agreement with AUC0-12 h by Bland-Altman analysis. CONCLUSION: The simplified MPA AUC formulae with 4-point sampling provide an effective approach for estimating full MPA AUC0-12 h in Chinese renal recipients on EC-MPS plus tacrolimus or cyclosporin A.
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Trasplante de Riñón , Ácido Micofenólico/análogos & derivados , Adulto , Área Bajo la Curva , Monitoreo de Drogas , Femenino , Humanos , Masculino , Persona de Mediana Edad , Ácido Micofenólico/farmacocinética , Trasplante Homólogo , Adulto JovenRESUMEN
An important component of amyloid fibrils in dialysis-related amyloidosis (DRA) is beta(2)-microglobulin (beta(2)m) modified with advanced glycation end products (AGE). The amyloid deposits are located principally in joint structures, with adjacent chronic inflammatory reaction characterized by monocyte infiltration. This study examined the interaction of AGE-beta(2)m with human synovial fibroblasts and investigated the proinflammatory effects of that interaction. It was demonstrated that human synovial fibroblasts constitutively expressed the receptor for AGE (RAGE). RAGE expression was detected mainly in synovial intima and was upregulated in DRA synovium. (125)I-AGE-beta(2)m bound to immobilized human synovial fibroblasts in a specific, dose-dependent manner (K(d) of approximately 138.0 nM), and binding was inhibited by anti-RAGE IgG. Incubation of human synovial fibroblasts with AGE-beta(2)m induced degradation of this AGE-modified protein, as well as increased monocyte chemoattractant protein-1 (MCP-1) mRNA and protein expression. The amount of MCP-1 produced by AGE-beta(2)m-stimulated human synovial fibroblasts was sufficient to induce the chemotaxis of monocytes. MCP-1 synthesis resulted from engagement of RAGE, because the increase in MCP-1 synthesis was attenuated by preincubation of human synovial fibroblasts with anti-RAGE IgG. These data provide evidence of RAGE-mediated perturbation of human synoviocytes, which may be involved in the pathogenesis of inflammatory processes associated with DRA.