RESUMEN
Psittacid herpesvirus type 1 (PsHV-1) was isolated from a captive galah (Eolophus roseicapillus) in Japan that was suspected of having Pacheco's disease (PD), an acute fatal disease in psittacine birds. PsHV-1 has been classified into four genotypes based on the UL16 gene sequence. In the present study, we investigated the genetic and pathogenic characteristics of the isolated virus, FOY-1, compared with a reference strain, RSL-1. The FOY-1 strain was classified into PsHV-1 genotype 2. The FOY-1 strain was found to be less pathogenic to budgerigars than RSL-1, which was classified as genotype 4 in an in vivo study. This is the first report regarding the classification of originally isolated PsHV-1 in Japan and its characterization by animal infection experiment.
Asunto(s)
Alphaherpesvirinae/genética , Enfermedades de las Aves/virología , Cacatúas/virología , ADN Viral/química , Infecciones por Herpesviridae/veterinaria , Alphaherpesvirinae/aislamiento & purificación , Alphaherpesvirinae/patogenicidad , Animales , Secuencia de Bases , Enfermedades de las Aves/mortalidad , Infecciones por Herpesviridae/mortalidad , Infecciones por Herpesviridae/virología , Japón , Melopsittacus/virología , Datos de Secuencia Molecular , FilogeniaRESUMEN
Although birds infected with avian polyomavirus (APV) subclinically could be a source of infection, no epidemiological studies of APV in psittacine birds have been reported in Japan. In the present study, we investigated subclinical morbidity rate of APV in imported and domestically bred psittacine birds by polymerase chain reaction (PCR). Of 402 live birds from which blood or feather samples were taken between April, 2003 and March, 2004, 11 (2.7%) were found to be APV positive. The DNA sequences of the APV t/T antigen region were determined for five APV-positive randomly selected samples and were found to be conserved.
