RESUMEN
Plants synthesize a plethora of chemical defence compounds, which vary between evolutionary lineages. We hypothesize that plants evolved the ability to utilize defence compounds synthesized and released by neighbouring heterospecific plants. In two experiments, we incubated clover (Trifolium repens L.) seedlings with individual benzoxazinoid (BX) compounds (2,4-dihydroxy-1,4-benzoxazin-3-one, 2-hydroxy-1,4-benzoxazin-3-one, benzoxazolinone, and 6-methoxy- benzoxazolin-2-one), a group of bioactive compounds produced by cereals, to allow clover BX uptake. Subsequently, we transplanted the seedlings into soil and quantified BX root and shoot content and invasion of root-knot nematodes in clover roots up to 8 weeks after transplantation. We show that clover root uptake of BXs substantially enhanced clover's resistance against the root-knot nematode Meloidogyne incognita. This effect lasted up to 6 weeks after the clover roots were exposed to the BXs. BXs were absorbed by clover roots, and then translocated to the shoots. As a result of clover metabolization, we detected the parent BXs and a range of their transformation products in the roots and shoots. Based on these novel findings, we envisage that co-cultivation of crop species with complementary and transferable chemical defence systems can add to plant protection.
Asunto(s)
Benzoxazinas , Raíces de Plantas , Trifolium , Tylenchoidea , Animales , Benzoxazinas/metabolismo , Raíces de Plantas/parasitología , Raíces de Plantas/metabolismo , Trifolium/metabolismo , Trifolium/parasitología , Tylenchoidea/fisiología , Enfermedades de las Plantas/parasitología , Grano Comestible/parasitología , Grano Comestible/metabolismo , Resistencia a la Enfermedad , Brotes de la Planta/metabolismo , Brotes de la Planta/parasitologíaRESUMEN
Metabolomic studies on root uptake and transformation of bioactive compounds, like cereal benzoxazinoids (BXs) in non-BX producing plants, are very limited. Therefore, a targeted mass-spectrometry-based metabolomics study was performed to elucidate the root uptake of BXs in white clover (Trifolium repens L.) and the impact of absorbed BXs on intrinsic clover secondary metabolites. Clover plants grew in a medium containing 100 µM of individual BXs (five aglycone and one glycoside BXs) for 3 weeks. Subsequently, plant tissues were analyzed by liquid chromatography-tandem mass spectrometry to quantify the BXs and clover secondary metabolite concentrations. All BXs were taken up by clover roots and translocated to the shoots. Upon uptake of 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA), 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA), 2-hydroxy-1,4-benzoxazin-3-one (HBOA), and 2-ß-d-glucopyranosyloxy-1,4-benzoxazin-3-one (HBOA-glc), the parent compounds and a range of transformation products were seen in the roots and shoots. The individual BX concentrations ranged from not detected (nd) to 469 µg/g of dry weight (dw) and from nd to 170 µg/g of dw in the roots and shoots, respectively. The root uptake of BXs altered the composition of intrinsic clover secondary metabolites. In particular, the concentration of flavonoids and the hormone abscisic acid increased substantially in comparison to control plants.
Asunto(s)
Benzoxazinas , Trifolium , Benzoxazinas/metabolismo , Grano Comestible/química , Ácido Abscísico/metabolismo , Trifolium/metabolismo , Medicago/metabolismo , Flavonoides/metabolismo , Raíces de Plantas/químicaRESUMEN
Phytotoxins are produced in plants including agricultural crops. Lupins and other plants of the Fabaceae family produce toxic alkaloids. These alkaloids have been studied in food and feed, however, the environmental fate of alkaloids produced by cultivated lupins is largely unknown. Therefore, we conducted an agricultural field experiment to investigate the occurrence of indole and quinolizidine alkaloids in lupin plant tissues, soil, soil pore water and in drainage water. During the field experiment, alkaloids were regularly quantified (median concentrations) in lupin (13-8.7 × 103 ng/g dry weight (dw)), and topsoils at depth 0-5 cm (0.1-10 ng/g dw), and depth 15-30 cm (0.2-8.5 ng/g dw), soil pore water (0.2-7.5 ng/L) and drainage water samples (0.4-18 ng/L). Lupanine was the dominant alkaloid in all collected samples. Cumulative amounts of alkaloids emitted via drainage water were around 0.1-11 mg/ha for individual alkaloids over one growing season. The total cumulative amount of alkaloid in drainage water was 14 mg/ha, which is a very small amount compared to the mass of alkaloid in the lupin biomass (11 kg/ha) and soil (0.02 kg/ha). Nearly half of the alkaloids were exported in the drainage water during high flow events, indicating that alkaloids transport preferentially via macropores. These findings indicate that drainage from lupin cultivated areas contribute to surface water contamination. The environmental and ecotoxicological relevance of alkaloids as newly identified aquatic micropollutants in areas with agricultural activities have yet to be assessed.
Asunto(s)
Alcaloides , Lupinus , Quinolizidinas , Indoles , Suelo , AguaRESUMEN
Phytotoxins - toxins produced by plants - are contaminants with the potential to impair drinking water quality. They encompass a large group of toxic, partially persistent compounds that have been detected in seepage waters and in shallow wells used for drinking water production. If phytotoxins enter wells used for drinking water production, it is essential to know if the drinking water treatment processes will remove them from the water phase. However, it is currently unknown whether phytotoxins remain stable during traditional groundwater treatment using sand filters as the main treatment process. The objective of this study is to investigate removal potential of phytotoxins in biological sand filters and to asses if the removal potential is similar at different waterworks. Microcosms were set up with filter sand and drinking water collected at different groundwater-based waterworks. To be able to monitor phytotoxin removal ptaquiloside, caudatoside, gramine, sparteine, jacobine N-oxide, senecionine N-oxide and caffeine were applied at initial concentrations of 300 µg L-1, which is approx. two orders of magnitude higher than currently detected in environment, but expected to cover extreme environmental conditions. Removal was monitored over a period of 14 days. Despite the high initial concentration, all filter sands removed ptaquiloside and caudatoside completely from the water phase and at waterworks where pellet softening was implemented (pH 8.4) prior to rapid sand filtration, complete removal occurred within the first 30 min. All filter sands removed gramine and sparteine, primarily by a biological process, while jacobine N-oxide, senecionine N-oxide and caffeine were recalcitrant in the filter sands. During degradation of ptaquiloside and caudatoside we observed formation and subsequent removal of degradation products pterosin B and A. Filter sands with the highest removal potential were characterised by high contents of deposited iron and manganese oxides and hence large specific surface areas. Difference between bacterial communities investigated by 16S rRNA gene analyses did not explain different removal in the filter sands. All five investigated filter sands showed similar degradation patterns regardless of water chemistry and waterworks of origin. In drinking water treatment systems biological sand filters might therefore remove phytotoxin contaminants such as ptaquiloside, caudatoside, gramine, sparteine, while for other compounds e.g. jacobine N-oxide, senecionine N-oxide further investigations involving more advanced treatment options are needed.
Asunto(s)
Agua Potable , Contaminantes Químicos del Agua , Purificación del Agua , Filtración , ARN Ribosómico 16S , Diálisis Renal , Arena , Dióxido de Silicio , Contaminantes Químicos del Agua/análisisRESUMEN
Phytotoxins are naturally produced toxins with potencies similar/higher than many anthropogenic micropollutants. Nevertheless, little is known regarding their environmental fate and off-field transport to streams. To fill this research gap, a network of six basins in the Midwestern United States with substantial soybean production was selected for the study. Stream water (n = 110), soybean plant tissues (n = 8), and soil samples (n = 16) were analyzed for 12 phytotoxins (5 alkaloids and 7 phytoestrogens) and 2 widely used herbicides (atrazine and metolachlor). Overall, at least 1 phytotoxin was detected in 82% of the samples, with as many as 11 phytotoxins detected in a single sample (median = 5), with a concentration range from below detection to 37 and 68 ng/L for alkaloids and phytoestrogens, respectively. In contrast, the herbicides were ubiquitously detected at substantially higher concentrations (atrazine: 99% and metolachlor: 83%; the concentrations range from below detection to 150 and 410 ng/L, respectively). There was an apparent seasonal pattern for phytotoxins, where occurrence prior to and during harvest season (September to November) and during the snow melt season (March) was higher than that in December-January. Runoff events increased phytotoxin and herbicide concentrations compared to those in base-flow conditions. Phytotoxin plant concentrations were orders of magnitude higher compared to those measured in soil and streams. These results demonstrate the potential exposure of aquatic and terrestrial organisms to soybean-derived phytotoxins.
RESUMEN
Heavy metal pollution in the environment has gained interest in the past few decades, as it has been found to accumulate in soil and water, as well as in the human body. Heavy metals present a serious health risk to humans when the concentration level and exposure time are increased. Although they have been extensively studied in the environment and food, little is known regarding their occurrence in the Kurdistan Region of northern Iraq. To fill this gap, we investigated the occurrence of some metals and heavy metals, namely aluminium (Al), barium (Ba), mercury (Hg), manganese (Mn), lead (Pb), vanadium (V) and zinc (Zn), in the blood of crude oil workers (40 men) who have worked in crude oil fields for at least 3 years-exposed group. In addition, a control group of 40 men was selected who have never worked or been close to crude oil fields. There is a significant correlation of metal concentrations between exposed and control groups, especially for Al, Ba, Hg, Mn and V (significant for all metals). Even though the correlation for Pb and Zn was weak, the concentrations were higher by 2-folds in the exposed group compared to the control group. Thus, the exposure caused an increase in the concentration of heavy metals by at least 1-fold in the blood of the exposed group compared to the control group. The environmental and ecotoxicological relevance of heavy metals in humans and the environment in areas with industrial activities have yet to be assessed.
Asunto(s)
Metales Pesados , Petróleo , Contaminantes del Suelo , China , Monitoreo del Ambiente , Humanos , Irak , Masculino , Metales Pesados/análisis , Medición de Riesgo , Contaminantes del Suelo/análisisRESUMEN
Pyrrolizidine alkaloids (PA)s are natural toxins produced by a variety of plants including ragwort. The PAs present a serious health risk to human and livestock. Although these compounds have been extensively studied in food and feed, little is known regarding their environmental fate. To fill this data gap, we investigated the occurrence of PAs in ragwort plants, soils and surface waters at three locations where ragwort was the dominant plant species to better understand their environmental distribution. The concentrations of PAs were quantified during the full growing season (April-November) and assessed in relation to rain events. PA concentrations ranged from 3.2-6.6 g/kg dry weight (dw) in plants, 0.8-4.0 mg/kg dw in soils, and 6.0-529 µg/L in surface waters. Maximum PA concentrations in the soil (4 mg/kg) and water (529 µg/L) were in mid-May just before flowering. The average distribution of PAs in water was approximately 5 g/10,000 L, compared to the average amounts present in ragwort (506 kg/ha), and soil (1.7 kg/ha). In general, concentrations of PAs increase in the soil and surface water following rain events.
Asunto(s)
Alcaloides de Pirrolicidina , Senecio , Pradera , Humanos , Lluvia , SueloRESUMEN
Pyrrolizidine alkaloids (PAs) are persistent mutagenic and carcinogenic compounds produced by many common plant species. Health authorities recommend minimising human exposure via food and medicinal products to ensure consumer health and safety. However, there is little awareness that PAs can contaminate water resources. Therefore, no regulations exist to limit PAs in drinking water. This study measured a PA base concentration of ~ 70 ng/L in stream water adjacent to an invasive PA-producing plant Petasites hybridus (Asteraceae). After intense rain the PA concentration increased tenfold. In addition, PAs measured up to 230 ng/L in seepage water from groundwater wells. The dominant PAs in both water types corresponded to the most abundant PAs in the plants (senkirkine, senecionine, senecionine N-oxide). The study presents the first discovery of persistent plant toxins in well water and their associated risks. In addition, it for the first time reports monocrotaline and monocrotaline N-oxide in Petasites sp.
RESUMEN
Pyrrolizidine alkaloids (PAs) are produced in plants as defence compounds against insects. PAs present a serious health risk to humans and livestock; therefore it is necessary to have a validated analytical method to monitor PAs in the environment. The objective of this work is to present an UPLC-MS/MS method for quantification of PAs in environmental samples of both soil and water. A fast, reliable, and sensitive approach is developed to identify and quantify PAs in soil and water. Sample preparation was performed by clean-up and pre-concentration of the samples using MCX solid phase extraction cartridges with full optimization, and then PAs were determined by UPLC coupled with TQ-MS. In the liquid chromatography, most of the parameters were optimized and tested including gradient time, solvents, additives, and pH of the mobile phases and flow rate. In addition, the MS parameters of cone voltage, desolvation temperature, cone flows, and collision energy were optimized. The instrument limit of detection (2-7 µg L-1) and limit of quantification (5-9 µg L-1) were determined experimentally, and the method was linearity validated up to 1000 µg L-1. The method was applied to analyse soil and surface water samples collected in April and May 2018 in Vejle, Borup, and Holte, Denmark. In total, 15 PAs were quantified and reported for the first time in environmental samples, in a range of 3-1349 µg kg-1 in soil and 4-270 µg L-1 in surface water.