Inula viscosa (L.) Aiton Ethanolic Extract Inhibits the Growth of Human AGS and A549 Cancer Cell Lines.

The present study shows the chemical profile and cytotoxic properties of the ethanolic extracts of Inula viscosa from Northeast Algeria. The extract was obtained by maceration using ethanol. Its phenolic profile was determined using ultra-high-performance liquid chromatography coupled with a diode array detector and an electrospray mass spectrometer (UHPLC-DAD-ESI/MS), which allowed the identification and quantification of 17 compounds, 1,5-O-caffeoylquinic acid being the most abundant. The cytotoxic activity was assessed against human gastric cancer (AGS) and human non-small-cell lung cancer (A549) cell lines, whereas ethanolic extract elicited nearly 60 % and 40 % viability loss toward AGS and A549 cancer cells, respectively. Results also showed that cell death is caspase-independent and confirmed the involvement of RIPK1 and the necroptosis pathway in the toxicity induced by the I. viscosa extract. In addition, the ethanolic extract would not provoke morphological traits in the cancer cells. These findings suggest that I. viscosa can be a source of new antiproliferative drugs or used in preparation plant-derived pharmaceuticals.

Chemical Composition and Antioxidant, Anti-Inflammatory, and Enzyme Inhibitory Activities of an Endemic Species from Southern Algeria: Warionia saharae.

Warionia saharae Benth. & Coss. (Asteraceae) is an endemic species of North Africa naturally grown in the southwest of the Algerian Sahara. In the present study, this species' hydromethanolic leaf extract was investigated for its phenolic profile characterized by ultra-high-performance liquid chromatography coupled with a diode array detector and an electrospray mass spectrometer (UHPLC-DAD-ESI/MS). Additionally, the chemical composition of W. saharae was analyzed by gas chromatography-mass spectrometry, and its antioxidant potential was assessed through five in vitro tests: DPPH● scavenging activity, ABTS●+ scavenging assay, galvinoxyl scavenging activity, ferric reducing power (FRP), and cupric reducing antioxidant capacity. The UHPLC-DAD-ESI/MS analysis allowed the detection and quantification of 22 compounds, with taxifolin as the dominant compound. The GC-MS analysis allowed the identification of 37 compounds, and the antioxidant activity data indicate that W. saharae extract has a very high capacity to capture radicals due to its richness in compounds with antioxidant capacity. The extract also showed potent α-glucosidase inhibition as well as a good anti-inflammatory activity. However, weak anti-α-amylase and anticholinesterase activities were recorded. Moreover, an in silico docking study was performed to highlight possible interactions between three significant compounds identified in W. saharae extract and α-glucosidase enzyme.

Methoxyfenozide, a Molting Hormone Agonist, Affects Autogeny Capacity, Oviposition, Fecundity, and Fertility in Culex pipiens (Diptera: Culicidae).

The current study aimed to evaluate the effects of methoxyfenozide (RH-2485), an insect growth disrupter (IGD) belonging to molting hormone agonist class, against female adults of Culex pipiens L. under laboratory conditions. Lethal concentrations (LC50 = 24.54 µg/liter and LC90 = 70.79 µg/liter), previously determined against fourth instar larvae, were tested for adult female fertility, fecundity and oviposition after tarsal contact before mating and any bloodmeal. Methoxyfenozide was found to alter negatively their autogeny capacity and oviposition. A strong reduction of 56% and 72% (P < 0.001) in females' autogeny capacity was observed in both treated series, respectively. Alteration in oviposition were found to be higher with LC90 (OAI-LC90 = -0.62) than with the LC50 (OAI-LC50 = -0.42). Also fecundity and hatching rate (fertility) were significantly reduced in treated series as compared to controls. A significant reduction of 37.65 and 28.23% in fecundity and decrease of 56.85 and 71.87% in fertility were found, respectively in LC50 and LC90 treated series. Obtained data clearly demonstrated that methoxyfenozide have significant depressive effect on reproductive potential against medically important vector with minimizing ecotoxicological risks in mosquitoes management.