Nicorandil attenuates cognitive impairment after traumatic brain injury via inhibiting oxidative stress and inflammation: Involvement of BDNF and NGF.

BACKGROUND AND PURPOSE: Cognitive impairment is a prevalent adverse consequence of traumatic brain injury (TBI). The neuroprotective effects of nicorandil (N-(2-hydroxyethyl)-nicotinamide nitrate) has been previously documented, yet its protective effects against cognitive dysfunction post-TBI remain unclear. Hence, the present study was aimed to evaluate whether nicorandil attenuates cognitive dysfunction in TBI rats and the underlying mechanism behind this process. METHODS: The TBI model was established with a controlled cortical impact (CCI). The effects of nicorandil on cognitive dysfunction of rats with TBI were examined through Novel object recognition (NOR) test, Y-maze test, and Morris water maze (MWM) task. After behavioral tests, hippocampal tissue was collected for Quantitative real-time PCR, Western blot analysis, and Enzyme-linked immunosorbent assay (ELISA) assay. RESULTS: We observed that nicorandil administration effectively ameliorates learning and memory impairment in TBI rats. Alongside, nicorandil treatment attenuated oxidative stress in the hippocampus of TBI rats, characterized by the decreased reactive oxygen species generation, malondialdehyde, and protein carbonyls levels, and concurrent promotion of antioxidant-related factors (including superoxide dismutase, glutathione peroxidase, and catalase) activities. Additionally, nicorandil treatment attenuated the inflammatory response in the hippocampus of TBI rat, as evidenced by the upregulated levels of interleukin (IL)-1ß, IL-6, and tumor necrosis factor-α (TNF-α), as well as the downregulated level of IL-10. Mechanistically, nicorandil treatment significantly enhanced the mRNA and protein levels of neurotrophic factors, brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in the hippocampus of TBI rats. CONCLUSION: These findings suggest that nicorandil mitigates cognitive impairment after TBI by suppressing oxidative stress and inflammation, potentially through enhancing BDNF and NGF levels.

Cultivation of aquaculture feed Isochrysis zhangjiangensis in low-cost wave driven floating photobioreactor without aeration device.

This study aimed to use floating photobioreactor (PBR) to produce microalgae biomass for aquaculture applications, and this was tested with cultivation of Isochrysis zhangjiangensis. The highest cell density of 16.1 ±â€¯0.61 × 106 cell L-1 was obtained in an outdoor culture with a depth of 5.0 cm in 1.0 m2 floating PBR, but deeper culture resulted in higher biomass productivity. Large-scale cultivation at size of 10 m2 (1000 L) produced the highest cell density of 17.8 × 106 cell L-1 and highest biomass productivity of 0.115 g L-1 d-1, which was at the same level as that for flat-panel PBR (100 L). This developed technique provides an innovative approach to produce microalgae on site for use as fresh aquaculture feed, as well as fresh cells for use as seed inoculums for large-area aquaculture water bodies. This approach provides not only a low-cost microalgae production system but also better integration between microalgae production and aquaculture.

Large-scale cultivation of Spirulina in a floating horizontal photobioreactor without aeration or an agitation device.

A low-cost floating photobioreactor (PBR) without the use of aeration and/or an agitation device, in which carbon was supplied in the form of bicarbonate and only wave energy was utilized for mixing, was developed in our previous study. Scaling up is a common challenge in the practical application of PBRs and has not yet been demonstrated for this new design. To fill this gap, cultivation of Spirulina platensis was conducted in this study. The results demonstrated that S. platensis had the highest productivity at 0.3 mol L-1 sodium bicarbonate, but the highest carbon utilization (104 ± 2.6%) was obtained at 0.1 mol L-1. Culture of Spirulina aerated with pure oxygen resulted in only minor inhibition of growth, indicating that its productivity will not be significantly reduced even if dissolved oxygen is accumulated to a high level due to intermittent mixing resulting from the use of wave energy. In cultivation using a floating horizontal photobioreactor at the 1.0 m2 scale, the highest biomass concentration of 2.24 ± 0.05 g L-1 was obtained with a culture depth of 5.0 cm and the highest biomass productivity of 18.9 g m-2 day-1 was obtained with a depth of 10.0 cm. This PBR was scaled up to 10 m2 (1000 L) with few challenges; biomass concentration and productivity during ocean testing were little different than those at the 1.0 m2 (100 L) scale. However, the larger PBR had an apparent carbon utilization efficiency of 45.0 ± 2.8%, significantly higher than the 39.4 ± 0.9% obtained at the 1 m2 scale. These results verified the ease of scaling up floating horizontal photobioreactors and showed their great potential in commercial applications.