RESUMEN
The ability to detect and visualize cellular events and associated biological analytes is essential for the understanding of their physiological and pathological functions. Cysteine (Cys) plays a crucial role in biological systems and lysosomal homeostasis. This puts forward higher requirements on the performance of the probe. Herein, we rationally designed a coumarin-based probe for the reversible, specific, sensitive, and rapid detection of Cys based on pH regulating reactivity. The obtained probe (ECMA) introduces a morpholine moiety to target lysosomes, and α,ß-unsaturated-ketone with an electron-withdrawing CN group served as a reversible reaction site for Cys. Importantly, ECMA was successfully applied to the real-time monitoring of Cys dynamics in living cells. Furthermore, cell imaging clearly revealed that exogenous Cys could induce the up-regulation of lysosomal ROS, which provided a powerful tool for investigating the relationship between oxidative stress and lysosomal Cys.
Asunto(s)
Cisteína , Colorantes Fluorescentes , Lisosomas , Estrés Oxidativo , Lisosomas/metabolismo , Lisosomas/química , Colorantes Fluorescentes/química , Colorantes Fluorescentes/síntesis química , Cisteína/química , Cisteína/metabolismo , Estrés Oxidativo/efectos de los fármacos , Humanos , Concentración de Iones de Hidrógeno , Células HeLa , Imagen Óptica , Estructura Molecular , Cumarinas/química , Especies Reactivas de Oxígeno/metabolismoRESUMEN
6-Nitrobenzo[cd]indole-2(1H)-ketone (compound C2) exhibits an excellent germicidal effect against methicillin-resistant Staphylococcus aureus (MRSA). Mechanism studies show that C2 induces ROS over-production, cell membrane damage, and ATP and virulence factor down-regulation in bacteria. More importantly, C2 can inhibit biofilm formation and accelerate wound healing in a mouse infection model induced by MRSA.
