Applicability of evaluation metrics/schemes for human health burden attributable to regional ozone pollution: A case study in the Guangdong-Hong Kong-Macao Greater Bay Area (GBA), South China.

This is a study to identify the applicable/preferable short- and long-term metrics/schemes to evaluate the premature mortality attributable to the ozone pollution in the Guangdong-Hong Kong-Macao Greater Bay Area (GBA), one of the most representative populous ozone pollution regions in China, by comprehensively accounting the uncertainty sources. The discrepancy between the observation and the CAQRA reanalysis datasets (2013-2019) was investigated in terms of the concentration variation pattern, which determines the exposure metric change. A set of domestic short-term C-R coefficients for the all-age population were integrated using the meta-analysis respectively corresponding to the metrics of MDA1, MDA8, and Daily average. The dataset-based deviations of the short-term attributable factors (AFs) and their corresponding premature mortalities were respectively about 16.9 ± 13.3 % and <5 % based on MDA8, much smaller than other two metrics; and the MDA8-based evaluation results were the most sensitive to the deteriorative ozone pollution, with the maximum upward trends of 0.095-0.129 %/year. Accordingly, MDA8 was recognized as the most applicable short-term metric. For the long-term exposure, the domestic summer metric SMDA8 could not exactly represent the peak-season ozone maximum level in the GBA, with the deviation from 6MMDA8 as much as 30 %. By considering the ability of metric to represent the peak-season ozone, the relatively smaller dataset-based discrepancies of AFs (6MMDA8-WHO2021: 23.3 ± 16.9 %, AMDA8-T2016: 20.7 ± 15.8 %) and the attributable premature mortalities (6MMDA8-WHO2021: 5 %, AMDA8-T2016: 8 %), and the higher sensitivity of the evaluation results to the deteriorative ozone pollution (6MMDA8-WHO2021: 0.13 %;year, p = 0.01; AMDA8-T2016: 0.15 %/year, p = 0.03), the schemes of 6MMDA8-WHO2021 and AMDA8-T2016 were recognized relatively more preferable for the adult (≥25-year) long-term evaluation. Based on the recognized metric/schemes, the central and the eastern PRE areas of higher NO2 level in the GBA were experiencing the highest health burdens from 2013 to 2019.

Water-energy-vegetation nexus explain global geographical variation in surface urban heat island intensity.

Surface urban heat island (SUHI) is a key climate risk associated with urbanization. Previous case studies have suggested that precipitation (water), radiation (energy), and vegetation have important effects on urban warming, but there is a lack of research that combines these factors to explain the global geographic variation in SUHI intensity (SUHII). Here, we utilize remotely sensed and gridded datasets to propose a new water-energy-vegetation nexus concept that explains the global geographic variation of SUHII across four climate zones and seven major regions. We found that SUHII and its frequency increase from arid zones (0.36 ± 0.15 °C) to humid zones (2.28 ± 0.10 °C), but become weaker in the extreme humid zones (2.18 ± 0.15 °C). We revealed that from semi-arid/humid to humid zones, high precipitation is often coupled with high incoming solar radiation. The increased solar radiation can directly enhance the energy in the area, leading to higher SUHII and its frequency. Although solar radiation is high in arid zones (mainly in West, Central, and South Asia), water limitation leads to sparse natural vegetation, suppressing the cooling effect in rural areas and resulting in lower SUHII. In extreme humid regions (mainly in tropical areas), incoming solar radiation tends to flatten out, which, coupled with increased vegetation as hydrothermal conditions become more favorable, leads to more latent heat and reduces the intensity of SUHI. Overall, this study offers empirical evidence that the water-energy-vegetation nexus highly explains the global geographic variation of SUHII. The results can be used by urban planners seeking optimal SUHI mitigation strategies and for climate change modeling work.

Influence of natural ventilation design on the dispersion of pathogen-laden droplets in a coach bus.

Natural ventilation is an energy-efficient design approach to reduce infection risk (IR), but its optimized design in a coach bus environment is less studied. Based on a COVID-19 outbreak in a bus in Hunan, China, the indoor-outdoor coupled CFD modeling approach is adopted to comprehensively explore how optimized bus natural ventilation (e.g., opening/closing status of front/middle/rear windows (FW/MW/RW)) and ceiling wind catcher (WCH) affect the dispersion of pathogen-laden droplets (tracer gas, 5 µm, 50 µm) and IR. Other key influential factors including bus speed, infector's location, and ambient temperature (Tref) are also considered. Buses have unique natural ventilation airflow patterns: from bus rear to front, and air change rate per hour (ACH) increases linearly with bus speed. When driving at 60 km/h, ACH is only 6.14 h-1 and intake fractions of tracer gas (IFg) and 5 µm droplets (IFd) are up to 3372 ppm and 1394 ppm with ventilation through leakages on skylights and no windows open. When FW and RW are both open, ACH increases by 43.5 times to 267.50 h-1, and IFg and IFd drop rapidly by 1-2 orders of magnitude compared to when no windows are open. Utilizing a wind catcher and opening front windows significantly increases ACH (up to 8.8 times) and reduces IF (5-30 times) compared to only opening front windows. When the infector locates at the bus front with FW open, IFg and IFd of all passengers are <10 ppm. More droplets suspend and further spread in a higher Tref environment. It is recommended to open two pairs of windows or open front windows and utilize the wind catcher to reduce IR in coach buses.

A portable acetylcholinesterase-based electrochemical sensor for field detection of organophosphorus.

A portable acetylcholinesterase (AChE)-based electrochemical sensor based on a screen-printed carbon electrode (SPCE) and a miniature potentiostat was constructed for the rapid field detection of organophosphorus pesticides (OPs). Graphene (GR) and gold nanoparticles (AuNPs) were successively introduced onto SPCE for surface modification. Due to the synergistic effect of the two nanomaterials, the signal of the sensor has a significant enhancement. Take isocarbophos (ICP) as a model for chemical warfare agents (CAWs) and Ops; the SPCE/GR/AuNPs/AChE/Nafion sensor shows a wider linear range (0.1-2000 µg L-1), and a lower limit of detection (0.012 µg L-1) than SPCE/AChE/Nafion and SPCE/GR/AChE/Nafion sensors. Tests in actual fruit and tap water samples also yielded satisfactory results. Therefore, the proposed method can be used as a simple and cost-effective strategy for construction of portable electrochemical sensors for OP field detection.

Particle Deposition in Large-Scale Human Tracheobronchial Airways Predicted by Single-Path Modelling.

The health effects of particles are directly related to their deposition patterns (deposition site and amount) in human airways. However, estimating the particle trajectory in a large-scale human lung airway model is still a challenge. In this work, a truncated single-path, large-scale human airway model (G3-G10) with a stochastically coupled boundary method were employed to investigate the particle trajectory and the roles of their deposition mechanisms. The deposition patterns of particles with diameters (dp) of 1-10 µm are investigated under various inlet Reynolds numbers (Re = 100-2000). Inertial impaction, gravitational sedimentation, and combined mechanism were considered. With the increasing airway generations, the deposition of smaller particles (dp < 4 µm) increased due to gravitational sedimentation, while that of larger particles decreased due to inertial impaction. The obtained formulas of Stokes number and Re can predict the deposition efficiency due to the combined mechanism in the present model, and the prediction can be used to assess the dose-effect of atmospheric aerosols on the human body. Diseases in deeper generations are mainly attributed to the deposition of smaller particles under lower inhalation rates, while diseases at the proximal generations mainly result from the deposition of larger particles under higher inhalation rates.

Conversational head movement decreases close-contact exposure to expired respiratory droplets.

People constantly move their heads during conversation, as such movement is an important non-verbal mode of communication. Head movement alters the direction of people's expired air flow, therefore affecting their conversational partners' level of exposure. Nevertheless, there is a lack of understanding of the mechanism whereby head movement affects people's exposure. In this study, a dynamic meshing method in computational fluid dynamics was used to simulate the head movement of a human-shaped thermal manikin. Droplets were released during the oral expiration periods of the source manikin, during which it was either motionless, was shaking its head or was nodding its head, while the head of a face-to-face target manikin remained motionless. The results indicate that the target manikin had a high level of exposure to respiratory droplets when the source manikin was motionless, whereas the target manikin's level of exposure was significantly reduced when the source manikin was shaking or nodding its head. The source manikin had the highest level of self-exposure when it was nodding its head and the lowest level of self-exposure when its head was motionless. People's level of exposure during close contact is highly variable, highlighting the need for further investigations in more realistic conversational scenarios.

Thermal perception and lung function: a panel study in young adults with exercise under high outdoor temperature.

It has been observed that high temperature exposure is associated with a reduction in lung function and some possible biological mechanisms have been suggested. However, it is unclear if thermal perception plays a role in the association. From September 3rd to 15th, 2018, in Guangzhou, China, we repeatedly measured daily thermal perception and lung function among 126 participants with outdoor military training. We performed a linear mixed model and stratified analyses by the origin of students, gender, and the training period to evaluate the effects of thermal perception on lung function. A total of 399 measurements were collected. Per vote increase in thermal sensation vote towards the "hot" direction was associated with a - 0.04 L (95% CI: - 0.08 to - 0.01) decrease in forced vital capacity (FVC), and - 0.04 L (95% CI: - 0.08 to - 0.01) decrease in forced expiratory volume in 1 s (FEV1). Per grade increase towards the "very uncomfortable" direction for thermal comfort vote was associated with an increased percentage of forced expiratory volume in 1 s (FEV1%) by 1.52% (95% CI: 0.18 to 2.86). For thermal preference, with preferred cooler vote increased by one level, FVC and FEV1 decreased by - 0.05 L/s (95% CI: - 0.08 to - 0.02) and - 0.05L/s (95% CI: - 0.08 to - 0.02), respectively. The effects of thermal perception on lung function were stronger among non-local and in the first week of training. Our study suggests that in the same high-temperature environment, thermal perception is associated with lung function, even in healthy adults.

Predominance of inhalation route in short-range transmission of respiratory viruses: Investigation based on computational fluid dynamics.

During the Coronavirus disease 2019 pandemic, short-range virus transmission has been observed to have a higher risk of causing infection than long-range virus transmission. However, the roles played by the inhalation and large droplet routes cannot be distinguished in practice. A recent analytical study revealed the predominance of short-range inhalation over the large droplet spray route as causes of respiratory infections. In the current study, short-range exposure was analyzed via computational fluid dynamics (CFD) simulations using a discrete phase model. Detailed facial membranes, including eyes, nostrils, and a mouth, were considered. In CFD simulations, there is no need for a spherical approximation of the human head for estimating deposition nor the "anisokinetic aerosol sampling" approximation for estimating inhalation in the analytical model. We considered two scenarios (with two spheres [Scenario 1] and two human manikins [Scenario 2]), source-target distances of 0.2 to 2 m, and droplet diameters of 3 to 1,500 µm. The overall CFD exposure results agree well with data previously obtained from a simple analytical model. The CFD results confirm the predominance of the short-range inhalation route beyond 0.2 m for expiratory droplets smaller than 50 µm during talking and coughing. A critical droplet size of 87.5 µm was found to differentiate droplet behaviors. The number of droplets deposited on the target head exceeded those exposed to facial membranes, which implies a risk of exposure through the immediate surface route over a short range. Electronic Supplementary Material ESM: the Supplementary Materials are available in the online version of this article at 10.1007/s12273-022-0968-y.

Aqueous-phase chemistry of atmospheric phenolic compounds: A critical review of laboratory studies.

Phenolic compounds (PhCs) are crucial atmospheric pollutants typically emitted by biomass burning and receive particular concerns considering their toxicity, light-absorbing properties, and involvement in secondary organic aerosol (SOA) formation. A comprehensive understanding of the transformation mechanisms on chemical reactions in atmospheric waters (i.e., cloud/fog droplets and aerosol liquid water) is essential to predict more precisely the atmospheric fate and environmental impacts of PhCs. Laboratory studies play a core role in providing the fundamental knowledge of aqueous-phase chemical transformations in the atmosphere. This article critically reviews recent laboratory advances in SOA formation from the aqueous-phase reactions of PhCs. It focuses primarily on the aqueous oxidation of PhCs driven by two atmospheric reactive species: OH radicals and triplet excited state organics, including the important chemical kinetics and mechanisms. The effects of inorganic components (i.e., nitrate and nitrite) and transition metal ions (i.e., soluble iron) are highlighted on the aqueous-phase transformation of PhCs and on the properties and formation mechanisms of SOA. The review is concluded with the current knowledge gaps and future perspectives for a better understanding of the atmospheric transformation and SOA formation potential of PhCs.

Transcutaneous auricular vagus nerve stimulation in poststroke cognitive impairment: protocol for a randomised controlled trial.

BACKGROUND: As one of the most common stroke sequelae, poststroke cognitive impairment significantly impacts 17.6%-83% of survivors, affecting their rehabilitation, daily living and quality of life. Improving cognitive abilities among patients in stroke recovery is therefore critical and urgent. Transcutaneous auricular vagus nerve stimulation (TAVNS) is a non-invasive, safe, cost-effective treatment with great potential for improving the cognitive function of poststroke patients. This clinical research will evaluate the effectiveness, and help elucidate the possible underlying mechanisms, of TAVNS for improving poststroke cognitive function. METHODS AND ANALYSIS: A single-centre, parallel-group, allocation concealment, assessor-blinded randomised controlled clinical trial. We will allocate 88 recruited participants to the TAVNS or sham group for an intervention that will run for 8 weeks, 5 days per week with twice daily sessions lasting 30 min each. Blood tests will be performed and questionnaires issued at baseline and 8-week and 12 week follow-ups. Primary outcomes will be changes in cognitive function scores. Secondary outcomes will be changes in activities of daily living, quality of life and serum oxidative stress indicators. ETHICS AND DISSEMINATION: The Ethics Committee of the First Affiliated Hospital of Hunan University of Chinese Medicine has approved the protocol (No. HN-LL-YJSLW-2022200). Findings will be published in peer-reviewed academic journals and presented at scientific conferences. TRIAL REGISTRATION NUMBER: ChiCTR2200057808.

Role of pathogen-laden expiratory droplet dispersion and natural ventilation explaining a COVID-19 outbreak in a coach bus.

The influencing mechanism of droplet transmissions inside crowded and poorly ventilated buses on infection risks of respiratory diseases is still unclear. Based on experiments of one-infecting-seven COVID-19 outbreak with an index patient at bus rear, we conducted CFD simulations to investigate integrated effects of initial droplet diameters(tracer gas, 5 µm, 50 µm and 100 µm), natural air change rates per hour(ACH = 0.62, 2.27 and 5.66 h-1 related to bus speeds) and relative humidity(RH = 35% and 95%) on pathogen-laden droplet dispersion and infection risks. Outdoor pressure difference around bus surfaces introduces natural ventilation airflow entering from bus-rear skylight and leaving from the front one. When ACH = 0.62 h-1(idling state), the 30-min-exposure infection risk(TIR) of tracer gas is 15.3%(bus rear) - 11.1%(bus front), and decreases to 3.1%(bus rear)-1.3%(bus front) under ACH = 5.66 h-1(high bus speed).The TIR of large droplets(i.e., 100 µm/50 µm) is almost independent of ACH, with a peak value(∼3.1%) near the index patient, because over 99.5%/97.0% of droplets deposit locally due to gravity. Moreover, 5 µm droplets can disperse further with the increasing ventilation. However, TIR for 5 µm droplets at ACH = 5.66 h-1 stays relatively small for rear passengers(maximum 0.4%), and is even smaller in the bus middle and front(<0.1%). This study verifies that differing from general rooms, most 5 µm droplets deposit on the route through the long-and-narrow bus space with large-area surfaces(L∼11.4 m). Therefore, tracer gas can only simulate fine droplet with little deposition but cannot replace 5-100 µm droplet dispersion in coach buses.

Influence of urban spatial and socioeconomic parameters on PM2.5 at subdistrict level: A land use regression study in Shenzhen, China.

The intraurban distribution of PM2.5 concentration is influenced by various spatial, socioeconomic, and meteorological parameters. This study investigated the influence of 37 parameters on monthly average PM2.5 concentration at the subdistrict level with Pearson correlation analysis and land-use regression (LUR) using data from a subdistrict-level air pollution monitoring network in Shenzhen, China. Performance of LUR models is evaluated with leave-one-out-cross-validation (LOOCV) and holdout cross-validation (holdout CV). Pearson correlation analysis revealed that Normalized Difference Built-up Index, artificial land fraction, land surface temperature, and point-of-interest (POI) numbers of factories and industrial parks are significantly positively correlated with monthly average PM2.5 concentrations, while Normalized Difference Vegetation Index and Green View Factor show significant negative correlations. For the sparse national stations, robust LUR modelling may rely on a priori assumptions in direction of influence during the predictor selection process. The month-by-month spatial regression shows that RF models for both national stations and all stations show significantly inflated mean values of R2 compared with cross-validation results. For MLR models, inflation of both R2 and R2CV was detected when using only national stations and may indicate the restricted ability to predict spatial distribution of PM2.5 levels. Inflated within-sample R2 also exist in the spatiotemporal LUR models developed with only national stations, although not as significant as spatial LUR models. Our results suggest that a denser subdistrict level air pollutant monitoring network may improve the accuracy and robustness in intraurban spatial/spatiotemporal prediction of PM2.5 concentrations.

Numerical Investigations of Urban Pollutant Dispersion and Building Intake Fraction with Various 3D Building Configurations and Tree Plantings.

Rapid urbanisation and rising vehicular emissions aggravate urban air pollution. Outdoor pollutants could diffuse indoors through infiltration or ventilation, leading to residents' exposure. This study performed CFD simulations with a standard k-ε model to investigate the impacts of building configurations and tree planting on airflows, pollutant (CO) dispersion, and personal exposure in 3D urban micro-environments (aspect ratio = H/W = 30 m, building packing density λp = λf = 0.25) under neutral atmospheric conditions. The numerical models are well validated by wind tunnel data. The impacts of open space, central high-rise building and tree planting (leaf area density LAD= 1 m2/m3) with four approaching wind directions (parallel 0° and non-parallel 15°, 30°, 45°) are explored. Building intake fraction is adopted for exposure assessment. The change rates of demonstrate the impacts of different urban layouts on the traffic exhaust exposure on residents. The results show that open space increases the spatially-averaged velocity ratio (VR) for the whole area by 0.40−2.27%. Central high-rise building (2H) can increase wind speed by 4.73−23.36% and decrease the CO concentration by 4.39−23.00%. Central open space and high-rise building decrease under all four wind directions, by 6.56−16.08% and 9.59−24.70%, respectively. Tree planting reduces wind speed in all cases, raising by 14.89−50.19%. This work could provide helpful scientific references for public health and sustainable urban planning.

Insufficient ventilation led to a probable long-range airborne transmission of SARS-CoV-2 on two buses.

Uncertainty remains on the threshold of ventilation rate in airborne transmission of SARS-CoV-2. We analyzed a COVID-19 outbreak in January 2020 in Hunan Province, China, involving an infected 24-year-old man, Mr. X, taking two subsequent buses, B1 and B2, in the same afternoon. We investigated the possibility of airborne transmission and the ventilation conditions for its occurrence. The ventilation rates on the buses were measured using a tracer-concentration decay method with the original driver on the original route. We measured and calculated the spread of the exhaled virus-laden droplet tracer from the suspected index case. Ten additional passengers were found to be infected, with seven of them (including one asymptomatic) on B1 and two on B2 when Mr. X was present, and one passenger infected on the subsequent B1 trip. B1 and B2 had time-averaged ventilation rates of approximately 1.7 and 3.2 L/s per person, respectively. The difference in ventilation rates and exposure time could explain why B1 had a higher attack rate than B2. Airborne transmission due to poor ventilation below 3.2 L/s played a role in this two-bus outbreak of COVID-19.

Predominant airborne transmission and insignificant fomite transmission of SARS-CoV-2 in a two-bus COVID-19 outbreak originating from the same pre-symptomatic index case.

The number of people infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to increase worldwide, but despite extensive research, there remains significant uncertainty about the predominant routes of SARS-CoV-2 transmission. We conducted a mechanistic modeling and calculated the exposure dose and infection risk of each passenger in a two-bus COVID-19 outbreak in Hunan province, China. This outbreak originated from a single pre-symptomatic index case. Some human behavioral data related to exposure including boarding and alighting time of some passengers and seating position and mask wearing of all passengers were obtained from the available closed-circuit television images/clips and/or questionnaire survey. Least-squares fitting was performed to explore the effect of effective viral load on transmission risk, and the most likely quanta generation rate was also estimated. This study reveals the leading role of airborne SARS-CoV-2 transmission and negligible role of fomite transmission in a poorly ventilated indoor environment, highlighting the need for more targeted interventions in such environments. The quanta generation rate of the index case differed by a factor of 1.8 on the two buses and transmission occurred in the afternoon of the same day, indicating a time-varying effective viral load within a short period of five hours.

Effects of tree plantings and aspect ratios on pedestrian visual and thermal comfort using scaled outdoor experiments.

Urban trees ameliorate heat stress for urban dwellers. However, it is difficult to quantitatively assess the integrated impacts of tree planting and street layouts on visual and thermal comfort in simulations and urban field experiments. We conducted scaled outdoor experiments in Guangzhou to investigate the influence of tree plantings on pedestrian visual and thermal comfort in street canyons with various aspect ratios (H/W = 1, 2, 3; H = 1.2 m). We considered the effects of tree crown covers (big and small crown) and tree planting densities (ρ = 1, 0.5) on pedestrian illuminance level and two thermal comfort indices (Physiological Equivalent Temperature: PET and Index of Thermal Stress: ITS). When ρ = 1, trees in most cases reduce pedestrian illuminance (maximum 140.0klux) and improve visual comfort. Decreasing ρ from 1 to 0.5 increases the illuminance (maximum 179.5klux) in the streets with big crown trees (H/W = 1, 2) and in the street with small crown trees (H/W = 2). When ρ = 1 (H/W = 1, 2), big crown trees decrease the peak daytime PET (by about 4.0 °C) and ITS (by about 285 W). Small crown trees (ρ = 1, H/W = 1, 2) produce a warming effect on peak daytime PET (2.0-3.0 °C), but a reduction in ITS is observed when H/W = 2, 3. After reducing ρ from 1 to 0.5, big crown trees increase peak daytime thermal stress according to both indices when H/W = 1, 2. Small crown trees exhibit a similar PET cycle between ρ = 0.5 and ρ = 1 across various H/W, but their daytime reduction of ITS is less effective when ρ = 0.5 (H/W = 2). The discrepancies between PET and ITS are attributed to their different approaches to modelling radiation fluxes. The narrower the street, the lower the illuminance, PET, and ITS, while their increases caused by reduced ρ are limited in narrow streets. Our study informs some potential urban tree planting strategies and produces high-quality validation data for numerical simulations and theoretical models.

Regional modeling of secondary organic aerosol formation over eastern China: The impact of uptake coefficients of dicarbonyls and semivolatile process of primary organic aerosol.

Capturing the secondary organic aerosol (SOA) concentration using the chemical transport model is difficult due to a large knowledge gap of its formation mechanism. Previous studies demonstrated the uptake of dicarbonyls and semivolatile process of primary organic aerosol (POA) emissions are the significant sources of SOA. However, the uptake coefficients of dicarbonyls have large uncertainties and the SOA from the semivolatile process of POA emission remains unclear. We applied the revised reactive uptake parameterization, with "salting effects" for dicarbonyls, and updated approaches for POA to the Community Multiscale Air Quality Modeling System (CMAQ) simulations for October 2014 to study their impacts on modeling the SOA formation over eastern China. We introduce a method of quantifying crystalized or deliquescent aerosols to further improve the parameterization. The revised glyoxal uptake coefficients results in higher glyoxal SOA in the Beijing-Tianjin-Hebei region, where is typically under low relative humidity (RH) and high aerosol pH conditions. It gives lower glyoxal SOA in the Pearl River Delta region, where is typically under high RH and low pH conditions. The updated parameterization gives negligible methylglyoxal SOA due to the low uptake coefficients. The implementation of semivolatile process of POA and the approach for potential SOA from combustion sources will largely decrease the predicted POA and increase the modeled SOA concentrations over eastern China. The increased SOA from POA emissions could improve the model performance for organic carbon and SOA. It slightly improves the performance in PM2.5 modeling by compensating the reduction of modeled POA. This study indicates the mixed impact of a parameterization considering "salting effects" on modeling the dicarbonyls SOA in key regions of eastern China. It also demonstrates the improved performance by implementing the POA approaches in aerosol modeling using CMAQ. Meanwhile, the uncertainty in the revised reactive uptake parameterization and POA approaches is discussed.

Evidence for lack of transmission by close contact and surface touch in a restaurant outbreak of COVID-19.

BACKGROUND: Coronavirus disease 2019 (COVID-19) is primarily a respiratory disease that has become a global pandemic. Close contact plays an important role in infection spread, while fomite may also be a possible transmission route. Research during the COVID-19 pandemic has identified long-range airborne transmission as one of the important transmission routes although lack solid evidence. METHODS: We examined video data related to a restaurant associated COVID-19 outbreak in Guangzhou. We observed more than 40,000 surface touches and 13,000 episodes of close contacts in the restaurant during the entire lunch duration. These data allowed us to analyse infection risk via both the fomite and close contact routes. RESULTS: There is no significant correlation between the infection risk via both fomite and close contact routes among those who were not family members of the index case. We can thus rule out virus transmission via fomite contact and interpersonal close contact routes in the Guangzhou restaurant outbreak. The absence of a fomite route agrees with the COVID-19 literature. CONCLUSIONS: These results provide indirect evidence for the long-range airborne route dominating SARS-CoV-2 transmission in the restaurant. We note that the restaurant was poorly ventilated, allowing for increasing airborne SARS-CoV-2 concentration.

Characterization of dicarboxylic acids, oxoacids, and α-dicarbonyls in PM2.5 within the urban boundary layer in southern China: Sources and formation pathways.

Low-molecular-weight dicarboxylic acids, which are important components of secondary organic aerosols, have been extensively studied in recent years. Many studies have focused on ground-level observations and literature reports on the vertical distribution of the organic aerosols within the urban boundary layer are limited. In this study, the vertical profiles of dicarboxylic acids and related organic compounds (DCRCs) in PM2.5 were investigated at altitudinal levels (ground level and 488 m above the ground level) at the Canton Tower in Guangzhou, southern China, to elucidate their primary sources and secondary formation processes. The concentrations of DCRCs at ground level were generally higher than those at 488 m. Oxalic acid (C2) was the most abundant species, followed by succinic acid (C4) and malonic acid (C3) at both heights. The higher ratio of DCRCs-bound carbon to organic carbon (i.e., DCRCs-C/OC) at 488 m (4.8 ± 1.2%) relative to that at ground level (2.7 ± 0.5%) indicated a higher degree of aerosol aging at 488 m. The abundance of C2 was increased and the conversion of C4 to C3 was enhanced due to the photochemical oxidation of its homologues during long-range transport periods. The increase in C2 was associated with in-cloud processes during pollution periods. Principal component analysis showed that DCRCs were mainly derived from atmospheric secondary processing and biomass burning was also an important source of long-chain carboxylic acids during autumn in Guangzhou. Our results illustrate that secondary processing and biomass burning play prominent roles in controlling the abundance of DCRCs. Furthermore, DCRCs are affected by air masses from regional areas, oxidation of their precursors via vertical transport and in-cloud processes.

Deposition of ambient particles in the human respiratory system based on single particle analysis: A case study in the Pearl River Delta, China.

It is important to evaluate how ambient particles are deposited in the human respiratory system in view of the adverse effects they pose to human health. Traditional methods of investigating human exposure to ambient particles suffer from drawbacks related either to the lack of chemical information from particle number-based measurements or to the poor time resolution of mass-based measurements. To address these issues, in this study, human exposure to ambient particulate matter was investigated using single particle analysis, which provided chemical information with a high time resolution. Based on single particle measurements conducted in the Pearl River Delta, China, nine particle types were identified, and EC (elemental carbon) particles were determined to be the most dominant type of particle. In general, the submicron size mode was dominant in terms of the number concentration for all of the particle types, except for Na-rich and dust particles. On average, around 34% of particles were deposited in the human respiratory system with 13.9%, 7.9%, and 12.6% being distributed in the head, tracheobronchial, and pulmonary regions, respectively. The amount of Na-rich particles deposited was the highest, followed by EC. The overall deposition efficiencies of the Na-rich and dust particles were higher than those of the other particle types due to their higher efficiencies in the head region, which could be caused by the greater sedimentation and impaction rates of larger particles. In the head region, the Na-rich particles made the largest contribution (30.5%) due to their high deposition efficiency, whereas in the tracheobronchial and pulmonary regions, EC made the largest contribution due to its high concentration. In summary, the findings of this initial trial demonstrate the applicability of single particle analysis to the assessment of human exposure to ambient particles and its potential to support traditional methods of analysis.