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1.
Biomed Rep ; 21(5): 161, 2024 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-39268408

RESUMEN

Nanoparticles (NPs) are one of the promising strategies to deal with bacterial infections. As the main subset of NPs, metal and metal oxide NPs show destructive power against bacteria by releasing metal ions, direct contact of cell membranes and antibiotic delivery. Recently, a number of researchers have focused on the antibacterial activity of zinc oxide nanoparticles (ZnO NPs) against Staphylococcus aureus (S. aureus). Currently, there is a lack of a comprehensive review on ZnO NPs against S. aureus. Therefore, in this review, the antibacterial activity against S. aureus of ZnO NPs made by various synthetic methods was summarized, particularly the green synthetic ZnO NPs. The synergistic antibacterial effect against S. aureus of ZnO NPs with antibiotics was also summarized. Furthermore, the present review also emphasized the enhanced activities against S. aureus of ZnO nanocomposites, nano-hybrids and functional ZnO NPs.

2.
Vascular ; 31(6): 1262-1264, 2023 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-35739076

RESUMEN

OBJECTIVE: To investigate the influence of peripheral artery disease (PAD) on the risk of mortality among coronavirus disease 2019 (COVID-19) patients based on adjusted effect estimates. METHODS: Systematic searches were performed through electronic databases. A random-effect model was applied to calculate the pooled effect and corresponding 95% confidence interval (CI). Inconsistency index (I2) was used to evaluate the heterogeneity across studies. Sensitivity analysis, subgroup analysis, and Begg's test were all implemented. RESULTS: On the basis of 16 eligible studies with 142,832 COVID-19 patients, the meta-analysis showed that PAD significantly increased the risk for mortality among COVID-19 patients (pooled effect = 1.29, 95% CI: 1.10-1.51). The significant association was also observed in the subgroup analysis stratified by hospitalized patients, mean age ≥ 60 years, Europe and North America. Sensitivity analysis verified the robustness of our findings. Begg's test (P = 0.15) showed there was no potential publication bias. CONCLUSIONS: COVID-19 patients with PAD may have a greater risk of mortality. Clinicians and nursing staff are supposed to identify and monitor these high-risk patients in a timely manner and provide appropriate clinical treatment for them.


Asunto(s)
COVID-19 , Enfermedad Arterial Periférica , Humanos , Persona de Mediana Edad , Bases de Datos Factuales , Europa (Continente) , Enfermedad Arterial Periférica/diagnóstico , Enfermedad Arterial Periférica/terapia , Metaanálisis como Asunto
3.
J Virol ; 96(17): e0078222, 2022 09 14.
Artículo en Inglés | MEDLINE | ID: mdl-36005760

RESUMEN

Members of the genus Hepacivirus have a broad range of hosts, with at least 14 species identified. To date, a highly pathogenic hepacivirus causing severe disease in animals has not been found. Here, by using high-throughput sequencing, a new hepacivirus was identified as the dominant and highly pathogenic virus in severe acute hepatitis outbreaks in bamboo rats (Rhizomys pruinosus), with ≈80% mortality; this virus emerged in February 2020 in two bamboo rat farms in China. Hepaciviral genome copies in bamboo rat liver were significantly higher than in other organs. Genomic sequences of hepacivirus strains from 12 sick bamboo rats were found to share 85.3 to 100% nucleotide (nt) identity and 94.9 to 100% amino acid (aa) identity and to share 79.7 to 87.8% nt and 90.4 to 97.8% aa identities with previously reported bamboo rat hepaciviruses of Vietnam and China. Sequence analysis further revealed the simultaneous circulation of genetically divergent hepacivirus variants within the two outbreaks. Phylogenetic analysis showed that hepacivirus strains from the present and previous studies formed an independent clade comprised of at least two genotypes, clearly different from all other known species, suggesting a novel species within the genus Hepacivirus. This is the first report of a non-human-infecting hepacivirus causing potentially fatal infection of bamboo rats, and the associated hepatitis in the animals potentially can be used to develop a surrogate model for the study of hepatitis C virus infection in humans and for the development of therapeutic strategies. IMPORTANCE Members of the genus Hepacivirus have a broad host range, with at least 14 species identified, but none is highly pathogenic to its host except for hepatitis C virus, which causes severe liver diseases in humans. In this study, a new liver-tropic hepacivirus species was identified by high-throughput sequencing as the pathogen associated with two outbreaks of severely acute hepatitis in hoary bamboo rats (Rhizomys pruinosus) on two farms in Hainan Province, China; this is the first reported highly pathogenic animal hepacivirus to our knowledge. Further phylogenetic analysis suggested that the hepaciviruses derived from hoary bamboo rats in either the current or previous studies represent a novel species within the genus Hepacivirus. This finding is a breakthrough that has significantly updated our understanding about the pathogenicity of animal hepaciviruses, and the hepacivirus-associated hepatitis in bamboo rats may have a use as an animal infection model to understand HCV infection and develop therapeutic strategies.


Asunto(s)
Hepacivirus , Hepatitis C , Animales , China/epidemiología , Brotes de Enfermedades , Hepacivirus/genética , Humanos , Filogenia
4.
Int Immunopharmacol ; 111: 109088, 2022 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-35921779

RESUMEN

OBJECTIVE: The aim of this study was to address the association between interstitial lung disease and the risk for severity and mortality among patients with coronavirus disease 2019 (COVID-19). METHODS: The electronic databases of PubMed, Web of Science and EMBASE were systematically searched. The pooled effect size with 95 % confidence interval (CI) was computed by a random-effects meta-analysis model. Heterogeneity test, sensitivity analysis, subgroup analysis, meta-regression analysis, Begg's test and Egger's test were performed. RESULTS: A total of sixteen eligible studies with 217,260 COVID-19 patients were enrolled in this meta-analysis. The findings based on adjusted effect estimates indicated that pre-existing interstitial lung disease was significantly associated with higher risk for COVID-19 severity (pooled effect = 1.34 [95 % CI: 1.16-1.55]) and mortality (pooled effect = 1.26 [95 % CI: 1.09-1.46]). Consistent results were observed in the subgroup analysis stratified by sample size, age, the percentage of male patients, study design, setting, the methods for adjustment and the factors for adjustment. The results of meta-regression demonstrated that sample size, age and region might be the potential sources of heterogeneity. Sensitivity analysis exhibited that our results were stable and robust. No publication bias was observed in Egger's test and Begg's test. CONCLUSION: This meta-analysis on the basis of adjusted effect estimates demonstrated that pre-existing interstitial lung disease was independently associated with significantly higher risk for COVID-19 severity and mortality.


Asunto(s)
COVID-19 , Enfermedades Pulmonares Intersticiales , Humanos , Masculino , Sesgo de Publicación
5.
Am J Emerg Med ; 58: 281-285, 2022 08.
Artículo en Inglés | MEDLINE | ID: mdl-35753290

RESUMEN

OBJECTIVE: This study aimed to evaluate whether there was a significant relationship between anemia and the risk for mortality among coronavirus disease 2019 (COVID-19) patients by a quantitative meta-analysis based on the adjusted effect estimates. METHODS: A systematic search was conducted in electronic databases to identify all published literature. A random-effects meta-analysis model was used to estimate the pooled effect size and 95% confidence interval (CI). Heterogeneity test, Begg's test, subgroup analysis and meta-regression were performed. RESULTS: Twenty-three articles with 573,928 COVID-19 patients were included in the quantitative meta-analysis. There was a significant association between anemia and an elevated risk of COVID-19 mortality (pooled effect size = 1.47, 95% CI [1.30-1.67]). We observed this significant association in the further subgroup analyses by age, proportion of males, sample size, study design, region and setting. Sensitivity analysis exhibited that our results were reliable. Begg's test showed that there was no publication bias. Meta-regression indicated that the tested variables might not be the source of heterogeneity. CONCLUSION: Our meta-analysis based on risk factors-adjusted effect estimates indicated that anemia was independently associated with a significantly elevated risk for mortality among COVID-19 patients.


Asunto(s)
Anemia , COVID-19 , Anemia/complicaciones , Anemia/epidemiología , Manejo de Datos , Humanos , Masculino , Factores de Riesgo
6.
Exp Ther Med ; 23(6): 415, 2022 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-35601071

RESUMEN

Aortic dissection is a serious acute cardiovascular disease with rapid onset, progression and a high mortality rate. Due to the range of different branching vessels involved, the clinical symptoms are complex and diverse. The typical clinical symptom is a severe tearing pain in the chest, back or abdomen, but some patients also have atypical symptoms, which are easily missed or misdiagnosed and can be life-threatening. The present study reports a case of painless type B aortic dissection, initially diagnosed as ileus. The objective of this study is to enhance the clinical understanding of painless aortic dissection so that the disease can be quickly and accurately detected, and treated in a timely manner, thereby improving patient outcomes.

8.
Autophagy ; 17(11): 3865-3874, 2021 11.
Artículo en Inglés | MEDLINE | ID: mdl-33446043

RESUMEN

Autophagic pathways are regulated mechanisms that play important roles in lysosome-mediated cellular degradation. Yet, the contribution of different autophagic pathways in lysosomal targeting, and characterization of the extent and specificity in their degradome remains largely uncharacterized. By undertaking a multiplex quantitative mass spectrometry approach, we have previously analyzed the lysosomal proteome during chaperone-mediated autophagy (CMA)-stimulated conditions in cancer cells. Here, we have extended our multiplex quantitative mass spectrometry and bioinformatics analysis on the proteome from isolated lysosomes to gain a comprehensive view of the temporal enriched lysosomal content upon non-macroautophagy-activated conditions. In parallel, we describe the functional dependency of LAMP2A on, and to what degree the presence of KFERQ-like motifs in proteins influences, their lysosomal targeting. These findings establish a framework for a better understanding of the degradome mediated by autophagic pathways beyond macroautophagy, and present characterization of the impact of LAMP2A in lysosomal targeting in cancer cells.Abbreviations: CMA: chaperone-mediated autophagy; ER: endoplasmic reticulum; EIF4A1: eukaryotic translation initiation factor 4A1; eMI: endosomal microautophagy; FC: fold change; GO: gene ontology; ISR: integrated stress response; LAMP2A: lysosomal associated membrane protein 2A; MA: macroautophagy; MI: microautophagy; MS: mass spectrometry; PCA: principal component analysis; TAX1BP1: Tax1 binding protein 1.


Asunto(s)
Proteína 2 de la Membrana Asociada a los Lisosomas/metabolismo , Lisosomas/metabolismo , Proteoma/metabolismo , Autofagia , Glucosa/deficiencia , Humanos , Proteómica
9.
Br J Cancer ; 123(7): 1164-1177, 2020 09.
Artículo en Inglés | MEDLINE | ID: mdl-32636467

RESUMEN

BACKGROUND: Deubiquitinating enzymes (DUBs) are linked to cancer progression and dissemination, yet less is known about their regulation and impact on epithelial-mesenchymal transition (EMT). METHODS: An integrative translational approach combining systematic computational analyses of The Cancer Genome Atlas cancer cohorts with CRISPR genetics, biochemistry and immunohistochemistry methodologies to identify and assess the role of human DUBs in EMT. RESULTS: We identify a previously undiscovered biological function of STAM-binding protein like 1 (STAMBPL1) deubiquitinase in the EMT process in lung and breast carcinomas. We show that STAMBPL1 expression can be regulated by mutant p53 and that its catalytic activity is required to affect the transcription factor SNAI1. Accordingly, genetic depletion and CRISPR-mediated gene knockout of STAMBPL1 leads to marked recovery of epithelial markers, SNAI1 destabilisation and impaired migratory capacity of cancer cells. Reversely, STAMBPL1 expression reprogrammes cells towards a mesenchymal phenotype. A significant STAMBPL1-SNAI1 co-signature was observed across multiple tumour types. Importantly, STAMBPL1 is highly expressed in metastatic tissues compared to matched primary tumour of the same lung cancer patient and its expression predicts poor prognosis. CONCLUSIONS: Our study provides a novel concept of oncogenic regulation of a DUB and presents a new role and predictive value of STAMBPL1 in the EMT process across multiple carcinomas.


Asunto(s)
Neoplasias de la Mama/patología , Transición Epitelial-Mesenquimal , Neoplasias Pulmonares/patología , Péptido Hidrolasas/fisiología , Línea Celular Tumoral , Enzimas Desubicuitinizantes/fisiología , Femenino , Humanos , Péptido Hidrolasas/análisis , Factores de Transcripción de la Familia Snail/análisis , Factores de Transcripción de la Familia Snail/fisiología , Proteína p53 Supresora de Tumor/genética
10.
Neural Netw ; 122: 308-319, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31739269

RESUMEN

High-frequency deep brain stimulation (HF-DBS) of the subthalamic nucleus (STN), globus pallidus interna (GPi) and globus pallidus externa (GPe) are often considered as effective methods for the treatment of Parkinson's disease (PD). However, the stimulation of a single nucleus by HF-DBS can cause specific physical damage, produce side effects and usually consume more electrical energy. Therefore, we use a biophysically-based model of basal ganglia-thalamic circuits to explore more effective stimulation patterns to reduce adverse effects and save energy. In this paper, we computationally investigate the combined DBS of two nuclei with the phase deviation between two stimulation waveforms (CDBS). Three different stimulation combination strategies are proposed, i.e., STN and GPe CDBS (SED), STN and GPi CDBS (SID), as well as GPi and GPe CDBS (GGD). Resultantly, it is found that anti-phase CDBS is more effective in improving parkinsonian dynamical properties, including desynchronization of neurons and the recovery of the thalamus relay ability. Detailed simulation investigation shows that anti-phase SED and GGD are superior to SID. Besides, the energy consumption can be largely reduced by SED and GGD (72.5% and 65.5%), compared to HF-DBS. These results provide new insights into the optimal stimulation parameter and target choice of PD, which may be helpful for the clinical practice.


Asunto(s)
Estimulación Encefálica Profunda/métodos , Globo Pálido/fisiopatología , Modelos Neurológicos , Enfermedad de Parkinson/terapia , Núcleo Subtalámico/fisiopatología , Tálamo/fisiopatología , Biofisica , Humanos , Neuronas/fisiología , Enfermedad de Parkinson/fisiopatología
11.
Autophagy ; 15(9): 1558-1571, 2019 09.
Artículo en Inglés | MEDLINE | ID: mdl-30821613

RESUMEN

Chaperone-mediated autophagy (CMA) is a lysosomal degradation pathway of select soluble proteins. Nearly one-third of the soluble proteins are predicted to be recognized by this pathway, yet only a minor fraction of this proteome has been identified as CMA substrates in cancer cells. Here, we undertook a quantitative multiplex mass spectrometry approach to study the proteome of isolated lysosomes in cancer cells during CMA-activated conditions. By integrating bioinformatics analyses, we identified and categorized proteins of multiple cellular pathways that were specifically targeted by CMA. Beyond verifying metabolic pathways, we show that multiple components involved in select biological processes, including cellular translation, was specifically targeted for degradation by CMA. In particular, several proteins of the translation initiation complex were identified as bona fide CMA substrates in multiple cancer cell lines of distinct origin and we show that CMA suppresses cellular translation. We further show that the identified CMA substrates display high expression in multiple primary cancers compared to their normal counterparts. Combined, these findings uncover cellular processes affected by CMA and reveal a new role for CMA in the control of translation in cancer cells. Abbreviations: 6-AN: 6-aminonicotinamide; ACTB: actin beta; AR7: atypical retinoid 7; CHX: cycloheximide; CMA: chaperone-mediated autophagy; CQ: chloroquine; CTS: cathepsins; DDX3X: DEAD-box helicase 3 X-linked; EEF2: eukaryotic translation elongation factor 2; EIF4A1: eukaryotic translation initiation factor 4A1; EIF4H: eukaryotic translation initiation factor 4H; GEO: Gene Expression Omnibus; GO: Gene Ontology; GSEA: gene set enrichment analysis; HK2: hexokinase 2; HSPA8/HSC70: heat shock protein family A (Hsp70) member 8; LAMP: lysosomal-associated membrane protein; LDHA: lactate dehydrogenase A; NES: normalized enrichment score; NFKBIA: NFKB inhibitor alpha; PCA: principle component analysis; PQ: paraquat; S.D.: standard deviation; SUnSET: surface sensing of translation; TMT: tandem mass tags; TOMM40/TOM40: translocase of outer mitochondrial membrane 40.


Asunto(s)
Autofagia Mediada por Chaperones/genética , Lisosomas/metabolismo , Neoplasias/metabolismo , Biosíntesis de Proteínas/genética , Proteoma/metabolismo , Línea Celular Tumoral , Autofagia Mediada por Chaperones/efectos de los fármacos , ARN Helicasas DEAD-box/genética , ARN Helicasas DEAD-box/metabolismo , Factor 4F Eucariótico de Iniciación/genética , Factor 4F Eucariótico de Iniciación/metabolismo , Factores Eucarióticos de Iniciación/genética , Factores Eucarióticos de Iniciación/metabolismo , Ontología de Genes , Proteínas del Choque Térmico HSC70/metabolismo , Humanos , Proteína 2 de la Membrana Asociada a los Lisosomas/metabolismo , Lisosomas/enzimología , Lisosomas/genética , Neoplasias/genética , Biosíntesis de Proteínas/efectos de los fármacos , Proteolisis , Proteoma/genética
12.
Life Sci Alliance ; 2(1)2019 02.
Artículo en Inglés | MEDLINE | ID: mdl-30718377

RESUMEN

RecQ-like helicase 4 (RECQL4) is mutated in patients suffering from the Rothmund-Thomson syndrome, a genetic disease characterized by premature aging, skeletal malformations, and high cancer susceptibility. Known roles of RECQL4 in DNA replication and repair provide a possible explanation of chromosome instability observed in patient cells. Here, we demonstrate that RECQL4 is a microtubule-associated protein (MAP) localizing to the mitotic spindle. RECQL4 depletion in M-phase-arrested frog egg extracts does not affect spindle assembly per se, but interferes with maintaining chromosome alignment at the metaphase plate. Low doses of nocodazole depolymerize RECQL4-depleted spindles more easily, suggesting abnormal microtubule-kinetochore interaction. Surprisingly, inter-kinetochore distance of sister chromatids is larger in depleted extracts and patient fibroblasts. Consistent with a role to maintain stable chromosome alignment, RECQL4 down-regulation in HeLa cells causes chromosome misalignment and delays mitotic progression. Importantly, these chromosome alignment defects are independent from RECQL4's reported roles in DNA replication and damage repair. Our data elucidate a novel function of RECQL4 in mitosis, and defects in mitotic chromosome alignment might be a contributing factor for the Rothmund-Thomson syndrome.


Asunto(s)
Metafase/genética , Proteínas Asociadas a Microtúbulos/genética , RecQ Helicasas/genética , RecQ Helicasas/metabolismo , Síndrome Rothmund-Thomson/enzimología , Animales , Cromatina/metabolismo , Inestabilidad Cromosómica/genética , Segregación Cromosómica/genética , Codón sin Sentido/genética , Reparación del ADN , Replicación del ADN , Mutación del Sistema de Lectura/genética , Células HEK293 , Células HeLa , Humanos , Cinetocoros/metabolismo , Microtúbulos/metabolismo , Óvulo/enzimología , Huso Acromático/enzimología , Xenopus/genética
13.
Biochem Biophys Res Commun ; 499(2): 314-320, 2018 05 05.
Artículo en Inglés | MEDLINE | ID: mdl-29574159

RESUMEN

Malate Dehydrogenase (MDH) 1 has recently been shown to be highly expressed and display prognostic value in non-small cell lung carcinomas (NSCLCs). However, it is not known how MDH1 expression is regulated and there is no current molecular or chemical strategy that specifically targets MDH1. This may be due to structural and enzymatic similarities with its isoenzyme, malate dehydrogenase 2 (MDH2). However, MDH1 and MDH2 are encoded by distinct genes and this opens up the possibility for modulation at the expression level. Here, we screened in silico for microRNAs (miRs) that selectively targets the 3'UTR region of MDH1. These analyses revealed that mir-126-5p has three binding sites in the 3'UTR region of MDH1. Additionally, we show that expression of miR-126-5p suppresses the enzymatic activity of MDH1, mitochondrial respiration and caused cell death in NSCLC cell lines.


Asunto(s)
Carcinoma de Pulmón de Células no Pequeñas/enzimología , Carcinoma de Pulmón de Células no Pequeñas/genética , Neoplasias Pulmonares/enzimología , Neoplasias Pulmonares/genética , Malato Deshidrogenasa/metabolismo , MicroARNs/metabolismo , Carcinoma de Pulmón de Células no Pequeñas/patología , Muerte Celular , Línea Celular Tumoral , Proliferación Celular , Respiración de la Célula , Células Clonales , Humanos , Neoplasias Pulmonares/patología , MicroARNs/genética , Mitocondrias/metabolismo
14.
J Appl Oral Sci ; 26: e20170113, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29489935

RESUMEN

Dental caries is a chronic progressive disease occurring in the tooth hard tissue due to multiple factors, in which bacteria are the initial cause. Both Streptococcus mutans and Streptococcus sanguinis are main members of oral biofilm. Helicobacter pylori may also be detected in dental plaque, playing an important role in the development of dental caries. OBJECTIVE: The aim of this study was to investigate the effect of H. pylori culture supernatant on S. mutans and S. sanguinis dual-species biofilm and to evaluate its potential ability on affecting dental health. MATERIAL AND METHODS: The effect of H. pylori supernatant on single-species and dual-species biofilm was measured by colony forming units counting and fluorescence in situ hybridization (FISH) assay, respectively. The effect of H. pylori supernatant on S. mutans and S. sanguinis extracellular polysaccharides (EPS) production was measured by both confocal laser scanning microscopy observation and anthrone-sulfuric acid method. The effect of H. pylori supernatant on S. mutans gene expression was measured by quantitative real-time PCR (qRT-PCR) assays. RESULTS: H. pylori supernatant could inhibit both S. mutans and S. sanguinis biofilm formation and EPS production. S. sanguinis inhibition rate was significantly higher than that of S. mutans. Finally, S. mutans bacteriocin and acidogenicity related genes expression were affected by H. pylori culture supernatant. CONCLUSION: Our results showed that H. pylori could destroy the balance between S. mutans and S. sanguinis in oral biofilm, creating an advantageous environment for S. mutans, which became the dominant bacteria, promoting the formation and development of dental caries.


Asunto(s)
Biopelículas , Placa Dental/microbiología , Helicobacter pylori/fisiología , Streptococcus mutans/fisiología , Streptococcus sanguis/fisiología , Recuento de Colonia Microbiana , Caries Dental/microbiología , Expresión Génica , Helicobacter pylori/genética , Hibridación Fluorescente in Situ , Microscopía Confocal , Plancton/crecimiento & desarrollo , Polisacáridos Bacterianos/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Streptococcus mutans/genética , Streptococcus sanguis/genética , Factores de Tiempo
15.
Oncol Lett ; 15(2): 2625-2630, 2018 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-29434983

RESUMEN

The aims of the present study were to identify key genes and pathways associated with hepatocellular carcinoma (HCC) progression and predict compounds potentially associated with this type of carcinogenesis. The gene expression profile data of the GSE49515 dataset was obtained from the Gene Expression Omnibus database. The limma software package was used to identify the differentially expressed genes (DEGs). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed using the Biological Networks Gene Ontology tool and the Database for Annotation, Visualization and Integrated Discovery, respectively. The Michigan Molecular Interactions database plugin within the Cytoscape software platform was used to perform protein-protein interaction (PPI) network analysis. Chemical-gene interaction data for HCC were obtained from the Comparative Toxicogenomics Database to evaluate the associations between drugs and specific genes. A total of 302 DEGs, including 231 downregulated and 71 upregulated, were identified. Cytokine-cytokine receptor interaction and chemokine signaling were the significantly enriched pathways. Additionally, PPI network analysis indicated a total of 13 highest degree hub nodes, including FBJ murine osteosarcoma viral oncogene homolog (FOS) and DNA damage-inducible transcript 3 protein (DDIT3). Chemical-gene interaction analysis revealed that FUN and FOS were targeted by >500 compounds, while >200 genes were targeted by 2,3,7,8-tetrachlorodibenzodioxin and benzo(α)pyrene. In conclusion, the present study demonstrated that FOS, DDIT3, the cytokine-cytokine receptor interaction pathway and the chemokine signaling pathway may be key genes and pathways associated with the development of HCC. Furthermore, exposure to 2,3,7,8-tetrachlorodibenzodioxin or benzo(α)pyrene may lead to hepatocarcinogenesis.

16.
J. appl. oral sci ; 26: e20170113, 2018. graf
Artículo en Inglés | LILACS, BBO | ID: biblio-893693

RESUMEN

Abstract Dental caries is a chronic progressive disease occurring in the tooth hard tissue due to multiple factors, in which bacteria are the initial cause. Both Streptococcus mutans and Streptococcus sanguinis are main members of oral biofilm. Helicobacter pylori may also be detected in dental plaque, playing an important role in the development of dental caries. Objective The aim of this study was to investigate the effect of H. pylori culture supernatant on S. mutans and S. sanguinis dual-species biofilm and to evaluate its potential ability on affecting dental health. Material and methods The effect of H. pylori supernatant on single-species and dual-species biofilm was measured by colony forming units counting and fluorescence in situ hybridization (FISH) assay, respectively. The effect of H. pylori supernatant on S. mutans and S. sanguinis extracellular polysaccharides (EPS) production was measured by both confocal laser scanning microscopy observation and anthrone-sulfuric acid method. The effect of H. pylori supernatant on S. mutans gene expression was measured by quantitative real-time PCR (qRT-PCR) assays. Results H. pylori supernatant could inhibit both S. mutans and S. sanguinis biofilm formation and EPS production. S. sanguinis inhibition rate was significantly higher than that of S. mutans. Finally, S. mutans bacteriocin and acidogenicity related genes expression were affected by H. pylori culture supernatant. Conclusion Our results showed that H. pylori could destroy the balance between S. mutans and S. sanguinis in oral biofilm, creating an advantageous environment for S. mutans, which became the dominant bacteria, promoting the formation and development of dental caries.


Asunto(s)
Streptococcus mutans/fisiología , Streptococcus sanguis/fisiología , Helicobacter pylori/fisiología , Biopelículas , Placa Dental/microbiología , Plancton/crecimiento & desarrollo , Polisacáridos Bacterianos/metabolismo , Streptococcus mutans/genética , Streptococcus sanguis/genética , Factores de Tiempo , Recuento de Colonia Microbiana , Expresión Génica , Helicobacter pylori/genética , Hibridación Fluorescente in Situ , Microscopía Confocal , Caries Dental/microbiología , Reacción en Cadena en Tiempo Real de la Polimerasa
17.
Sci Rep ; 7(1): 7206, 2017 08 03.
Artículo en Inglés | MEDLINE | ID: mdl-28775282

RESUMEN

Dental caries is closely associated with the microbial dybiosis between acidogenic/aciduric pathogens and alkali-generating commensal bacteria colonized in the oral cavity. Our recent studies have shown that arginine may represent a promising anti-caries agent by modulating microbial composition in an in vitro consortium. However, the effect of arginine on the oral microbiota has yet to be comprehensively delineated in either clinical cohort or in vitro biofilm models that better represent the microbial diversity of oral cavity. Here, by employing a clinical cohort and a saliva-derived biofilm model, we demonstrated that arginine treatment could favorably modulate the oral microbiota of caries-active individuals. Specifically, treatment with arginine-containing dentifrice normalized the oral microbiota of caries-active individuals similar to that of caries-free controls in terms of microbial structure, abundance of typical species, enzymatic activities of glycolysis and alkali-generation related enzymes and their corresponding transcripts. Moreover, we found that combinatory use of arginine with fluoride could better enrich alkali-generating Streptococcus sanguinis and suppress acidogenic/aciduric Streptococcus mutans, and thus significantly retard the demineralizing capability of saliva-derived oral biofilm. Hence, we propose that fluoride and arginine have a potential synergistic effect in maintaining an eco-friendly oral microbial equilibrium in favor of better caries management.


Asunto(s)
Arginina/farmacología , Microbiota/efectos de los fármacos , Boca/microbiología , Biopelículas/efectos de los fármacos , Caries Dental/tratamiento farmacológico , Caries Dental/microbiología , Disbiosis , Humanos , ARN Ribosómico 16S/genética , Saliva/microbiología , Streptococcus sanguis/efectos de los fármacos , Streptococcus sanguis/genética , Streptococcus sanguis/metabolismo
18.
Methods Mol Biol ; 1475: 41-54, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-27631796

RESUMEN

One of the few proteins that have SUMO E3 ligase activity is the 358 kDa nucleoporin RanBP2 (Nup358). While small fragments of RanBP2 can stimulate SUMOylation in vitro, the physiologically relevant E3 ligase is a stable multi-subunit complex comprised of RanBP2, SUMOylated RanGAP1, and Ubc9. Here, we provide a detailed protocol to in vitro reconstitute the RanBP2 SUMO E3 ligase complex. With the exception of RanBP2, reconstitution involves untagged full-length proteins. We describe the bacterial expression and purification of all complex components, namely an 86 kDa His-tagged RanBP2 fragment, the SUMO E2-conjugating enzyme Ubc9, RanGAP1, and SUMO1, and we provide a protocol for quantitative SUMOylation of RanGAP1. Finally, we present details for the assembly and final purification of the catalytically active RanBP2/RanGAP1*SUMO1/Ubc9 complex.


Asunto(s)
Proteínas Activadoras de GTPasa/metabolismo , Chaperonas Moleculares/metabolismo , Proteínas de Complejo Poro Nuclear/metabolismo , Procesamiento Proteico-Postraduccional , Proteómica/métodos , Proteína SUMO-1/metabolismo , Enzimas Ubiquitina-Conjugadoras/metabolismo , Clonación Molecular , Escherichia coli/genética , Escherichia coli/metabolismo , Proteínas Activadoras de GTPasa/genética , Expresión Génica , Vectores Genéticos/química , Vectores Genéticos/metabolismo , Humanos , Chaperonas Moleculares/genética , Proteínas de Complejo Poro Nuclear/genética , Unión Proteica , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Proteína SUMO-1/genética , Sumoilación , Enzimas Ubiquitina-Conjugadoras/genética
19.
Diagn Pathol ; 8: 69, 2013 Apr 30.
Artículo en Inglés | MEDLINE | ID: mdl-23631806

RESUMEN

OBJECTIVE: Accumulating evidence for differential expression of microRNA-224 (miR-224) in various types of human cancer suggests that it may be play a crucial role in tumor biology. The previous microarray detection also shown that miR-224 was one of miRNAs with significant upregulation in cervical cancer tissues relative to adjacent normal tissues. However, little is known about the function of miR-224 in human cervical cancer. The aim of this study was to investigate the clinical significance of miR-224 expression in cervical cancer. METHODS: MiR-224 expression in 126 pairs of fresh human cervical cancer and adjacent normal tissues was measured by real-time quantitative RT-PCR assay. RESULTS: miR-224 expression was significantly upregulated in cervical cancer tissues when compared with corresponding adjacent normal tissues (P<0.001). It was also significantly higher in the cancerous tissues of patients with advanced FIGO stage cervical cancer than those with early FIGO stage (P=0.02). In addition, miR-224 was expressed at significantly higher levels in lymph node metastasis-positive patients than in lymph node metastasis-negative patients (P=0.008). Moreover, we found that lesser differentiated tumors expressed higher miR-224 (P=0.03). Finally, there were sufficient evidence to confirm its value in the status of vascular invasion (P=0.01) and human papillomavirus (HPV) infection (P=0.02) in cervical cancer. More importantly, Kaplan-Meier analysis showed that cervical cancer patients with high miR-224 expression tend to have shorter overall survival. In multivariate analysis stratified for known prognostic variables, miR-224 was identified as an independent prognostic marker. CONCLUSION: Our data indicated that miR-224 upregulation was associated with aggressive progression and poor prognosis in cervical cancer. MiR-224 was identified for the first time as an independent marker for predicting the clinical outcome of cervical cancer patients. VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2170449349527493.


Asunto(s)
Biomarcadores de Tumor/genética , Regulación Neoplásica de la Expresión Génica/genética , MicroARNs/genética , Neoplasias del Cuello Uterino/genética , Adulto , Anciano , Progresión de la Enfermedad , Femenino , Humanos , MicroARNs/metabolismo , Persona de Mediana Edad , Pronóstico , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Regulación hacia Arriba/fisiología , Neoplasias del Cuello Uterino/diagnóstico , Neoplasias del Cuello Uterino/patología
20.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 30(5): 493-7, 2012 Oct.
Artículo en Chino | MEDLINE | ID: mdl-23173313

RESUMEN

OBJECTIVE: To analyze the mechanism of fluorine by systemic analysis of fluorination-demineralization-remineralization experiments. METHODS: The enamel specimens were randomly assigned to untreated group (group A), non-fluoride group (group B), low-fluoride group (group C) and high-fluoride group (group D). The in vitro model of fluoride enamel was established in group C and D. Based on that, the establishment of demineralization model and remineralization experiment by pH-cycling in group B, C and D were followed. All enamel specimens were observed by stereomicroscope and scanning electron microscope and compared in surface microhardness value. RESULTS: There was distinct difference in micro-morphologic appearance on fluoride enamel surface. Artificial caries of fluoride enamel showed a relatively complete surface, the surface microhardness after demineralization and remineralization in fluoride group was higher than non-fluoride group (P < 0.05). CONCLUSION: The fluorinated enamel can enhance cariostatic potential and remineralization capacity of dental enamel.


Asunto(s)
Flúor , Remineralización Dental , Caries Dental , Esmalte Dental , Fluoruros , Humanos , Fosfatos , Desmineralización Dental
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