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The solution aggregation structure of conjugated polymers is crucial to the morphology and resultant optoelectronic properties of organic electronics and is of considerable interest in the field. Precise characterizations of the solution aggregation structures of organic photovoltaic (OPV) blends and their temperature-dependent variations remain challenging. In this work, the temperature-dependent solution aggregation structures of three representative high-efficiency OPV blends using small-angle X-ray/neutron scattering are systematically probed. Three cases of solution processing resiliency are elucidated in state-of-the-art OPV blends. The exceptional processing resiliency of high-efficiency PBQx-TF blends can be attributed to the minimal changes in the multiscale solution aggregation structure at elevated temperatures. Importantly, a new parameter, the percentage of acceptors distributed within polymer aggregates (Ф), for the first time in OPV blend solution, establishes a direct correlation between Ф and performance is quantified. The device performance is well correlated with the Kuhn length of the cylinder related to polymer aggregates L1 at the small scale and the Ф at the large scale. Optimal device performance is achieved with L1 at ≈30 nm and Ф within the range of 60 ± 5%. This study represents a significant advancement in the aggregation structure research of organic electronics.
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The controlled peptide self-assembly and disassembly are not only implicated in many cellular processes but also possess huge application potential in a wide range of biotechnology and biomedicine. ß-sheet peptide assemblies possess high kinetic stability, so it is usually hard to disassemble them rapidly. Here, we reported that both the self-assembly and disassembly of a designed short ß-sheet peptide IIIGGHK could be well harnessed through the variations of concentration, pH, and mechanical stirring. Microscopic imaging, neutron scattering, and infrared spectroscopy were used to track the assembly and disassembly processes upon these stimuli, especially the interconversion between thin, left-handed protofibrils and higher-order nanotubes with superstructural right-handedness. The underlying rationale for these controlled disassembly processes mainly lies in the fact that the specific His-His interactions between protofibrils were responsive to these stimuli. By taking advantage of the peptide self-assembly and disassembly, the encapsulation of the hydrophobic drug curcumin and its rapid release upon stimuli were achieved. Additionally, the peptide hydrogels facilitated the differentiation of neural cells while maintaining low cell cytotoxicity. We believe that such dynamic and reversible structural transformation in this work provides a distinctive paradigm for controlling the peptide self-assembly and disassembly, thus laying a foundation for practical applications of peptide assemblies.
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Nanotubos de Péptidos , Nanotubos , Nanotubos de Péptidos/química , Péptidos/farmacología , Péptidos/química , Conformación Proteica en Lámina betaRESUMEN
Benefiting from the photovoltaic material innovation and delicate device optimization, high-efficiency solar cells employing polymeric materials are thriving. Reducing the gap of cost, efficiency, and stability is the critical challenge faced by the emerging solar cells such as organics, quantum dots and perovskites. Poly(3-alkylthiophene) demonstrates great potential in organic solar cells and quantum dot solar cells as the active layer or the hole transport layer due to its large scalability, excellent photoelectric performance, and favorable hydrophobicity. The present low efficiency and insufficient stability, restrict its commercial application. In this work, a facile strategy of blending two simple polythiophenes is put forward to manipulate the film microstructure and enhance the device efficiency and thermal stability of solar cells. The introduction of P3PT can improve the power conversion efficiency (PCE) of a benchmark cost-effective blend P3HT:O-IDTBR to 7.41%, and the developed ternary solar cells also exhibit increased thermal stability. More strikingly, the quantum dot solar cells with the dual-polythiophene hole transport layer achieve the highest PCE of 10.51%, which is among the topmost efficiencies for quantum dots/polythiophene solar cells. Together, this work provides an effective route to simultaneously optimize the device efficiency and thermal stability of solar cells.
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Organic solar cells (OSCs) have potential for applications in wearable electronics. Except for high power conversion efficiency (PCE), excellent tensile properties and mechanical stability are required for achieving high-performance wearable OSCs, while the present metrics barely meet the stretchable requirements. Herein, this work proposes a facile and low-cost strategy for constructing intrinsically stretchable OSCs by introducing a readily accessible polymer elastomer as a diluent for all-polymer photovoltaic blends. Remarkably, record-high stretchability with a fracture strain of up to 1000% and mechanical stability with elastic recovery >90% under cyclic tensile tests are realized in the OSCs active layers for the first time. Specifically, the tensile properties of best-performing all-polymer photovoltaic blends are increased by up to 250 times after blending. Previously unattainable performance metrics (fracture strain >50% and PCE >10%) are achieved simultaneously for the resulting photovoltaic films. Furthermore, an overall evaluation parameter y is proposed for the efficiency-cost- stretchability balance of photovoltaic blend films. The y value of dilute-absorber system is two orders of magnitude greater than those of prior state-of-the-art systems. Additionally, intrinsically stretchable devices are prepared to showcase the mechanical stability. Overall, this work offers a new avenue for constructing and comprehensively evaluating intrinsically stretchable organic electronic films.
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Surfactant-like short peptides are a kind of ideal model for the study of chiral self-assembly. At present, there are few studies on the chiral self-assembly of multicharged surfactant-like peptides. In this study, we adopted a series of short peptides of Ac-I4KGK-NH2 with different combinations of L-lysine and D-lysine residues as the model molecules. TEM, AFM and SANS results showed that Ac-I4LKGLK-NH2, Ac-I4LKGDK-NH2, and Ac-I4DKGLK-NH2 formed the morphologies of nanofibers, and Ac-I4DKGDK-NH2 formed nanoribbons. All the self-assembled nanofibers, including the intermediate nanofibers of Ac-I4DKGDK-NH2 nanoribbons, showed the chirality of left handedness. Based on the molecular simulation results, it has been demonstrated that the supramolecular chirality was directly dictated by the orientation of single ß strand. The insertion of glycine residue demolished the effect of lysine residues on the single strand conformation due to its high conformational flexibility. The replacement of L-isoleucine with Da-isoleucine also confirmed that the isoleucine residues involved in the ß-sheet determined the supramolecular handedness. This study provides a profound mechanism of the chiral self-assembly of short peptides. We hope that it will improve the regulation of chiral molecular self-assembly with achiral glycine, as well.
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Nanofibras , Nanotubos de Carbono , Surfactantes Pulmonares , Nanofibras/química , Glicina , Tensoactivos/química , Lisina/química , Isoleucina , Lateralidad Funcional , Péptidos/química , LipoproteínasRESUMEN
A small-angle neutron scattering (SANS) instrument at the China Spallation Neutron Source (CSNS) is an operating instrument for studying structures and inhomogeneities with dimensions ranging from 1 to 100 nm. Preparing multiple samples at once and measuring them sequentially is a common approach in SANS experiments to reduce neutron beamline wastes and increase experimental efficiency. We present the development of an automatic sample changer for the SANS instrument, including system design, thermal simulation, optimization analysis, structure design details, and temperature control test results. It features a two-row construction that can hold 18 samples on each row. The controllable temperature range is -30 to 300 °C. Furthermore, neutron scattering experiments on SANS at CSNS proved that this instrument has good temperature control performance and low background. This automatic sample changer is optimized for usage at SANS and will be offered to other researchers through the user program.
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Neutrones , Sincrotrones , Temperatura , Dispersión del Ángulo Pequeño , Simulación por Computador , Difracción de NeutronesRESUMEN
The formation of reactive metabolites (RMs) is thought to be one of the pathogeneses for some idiosyncratic adverse drug reactions (IADRs) which are considered one of the leading causes of some drug attritions and/or recalls. Minimizing or eliminating the formation of RMs via chemical modification is a useful tactic to reduce the risk of IADRs and time-dependent inhibition (TDI) of cytochrome P450 enzymes (CYPs). The RMs should be carefully handled before making a go-no-go decision. Herein, we highlight the role of RMs in the occurrence of IADRs and CYP TDI, the risk of structural alerts, the approaches of RM assessment at the discovery stage and strategies to minimize or eliminate RM liability. Finally, some considerations for handling a RM-positive drug candidate are suggested.
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Efectos Colaterales y Reacciones Adversas Relacionados con Medicamentos , Humanos , Efectos Colaterales y Reacciones Adversas Relacionados con Medicamentos/prevención & control , Descubrimiento de Drogas , Sistema Enzimático del Citocromo P-450 , Evaluación Preclínica de MedicamentosRESUMEN
HYPOTHESIS: Self-assembly of peptides is influenced by both molecular structure and external conditions, which dictate the delicate balance of different non-covalent interactions that driving the self-assembling process. The shifting of terminal charge residue is expected to influence the non-covalent interactions and their interplay, thereby affecting the morphologies of self-assemblies. Therefore, the morphology transition can be realized by shifting the position of the terminal charge residue. EXPERIMENTS: The structure transition from thin nanofibers to giant nanotubes is realized by simply shifting the C-terminal lysine of ultrashort Ac-I3K-NH2 to its N-terminus. The morphologies and detailed structure information of the self-assemblies formed by these two peptides are investigated systemically by a combination of different experimental techniques. The effect of terminal residue on the morphologies of the self-assemblies is well presented and the underlying mechanism is revealed. FINDINGS: Giant nanotubes with a bilayer shell structure can be self-assembled by the ultrashort peptide Ac-KI3-NH2 with the lysine residue close to the N-terminal. The Ac-KI3-NH2 dimerization through intermolecular C-terminal H-bonding promotes the formation of a bola-form geometry, which is responsible for the wide nanotube assembly formation. The evolution process of Ac-KI3-NH2 nanotubes follows the "growing width" model. Such a morphological transformation with the terminal lysine shift is applicable to other analogues and thus provides a facile approach for the self-assembly of wide peptide nanotubes, which can expand the library of good template structures for the prediction of peptide nanostructures.
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Nanotubos de Péptidos , Nanotubos , Estructura Secundaria de Proteína , Lisina , Nanotubos/química , Péptidos/químicaRESUMEN
Lignin nanoparticles (LNPs) are usually produced from lignin solution through supersaturation. The structure of the lignin in solution is still poorly understood due to structural variability of isolated lignins. Here, lignins were extracted from different plants to establish a general pattern of their structure in several lignin solvents. Lignin molecules (lignin subunits) and larger aggregates were observed in dimethyl sulfoxide (DMSO), ethylene glycol (EG) and 0.1â N NaOD solutions by small-angle neutron scattering (SANS). It was proposed that the aggregates were composed of lignin subunits with a higher molecular weight and a higher ratio of the aliphatic to phenolic hydroxyl groups. The size, shape, and compactness are important factors that affect the uses of the LNPs, which were obtained from the SANS data for the first time. A discrepancy in the radius between SANS and DLS was discovered, pointing to a large hydration shell around the LNPs in aqueous solutions. The cytotoxicity of the corncob lignin, kraft lignin, and their LNPs were measured and compared.
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Lignina , Nanopartículas , Dimetilsulfóxido , Glicoles de Etileno , Lignina/química , Dispersión del Ángulo Pequeño , Solventes/químicaRESUMEN
The charge carrier transport of conjugated polymer thin film is mainly decided by the crystalline domain and intercrystallite connection. High-density tie-chain can provide an effective bridge between crystalline domains. Herein, the tie-chain connection behavior is optimized by decreasing the crystal region length (lc ) and increasing the crystallization rate. Poly[4-(4,4-bis(2-octyldodecyl)-4H-cyclopenta[1,2-b:5,4-b']dithiophen-2-yl)-alt-[1,2,5]-thiadiazolo[3,4-c]pyridine] (PCDTPT-ODD) is dissolved in nonpolar solvent isooctane and high ordered rod-like aggregations are formed. As the temperature increases, the changes in solution state and crystallization behavior lead to three different chain arrangement morphologies in the films: 1) at 25 °C, large and separated crystal regions are formed; 2) at 55 °C, small and well-connected crystal regions are formed due to faster crystallization rate and smaller nucleus size; 3) at 90 °C, the amorphous film is formed. Further results show that the film prepared at 55 °C has a smaller crystal region length (lc , 7.6 nm) and higher tie-chains content. Thus, the film exhibits the best device mobility of 2.3 × 10-3 cm2 V-1 s-1 . This result shows the great influence of crystallization kinetics on the microstructure of conjugated polymer films and provides an effective way for the optimization of the intercrystallite tie-chain.
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The elastic storage and release of mechanical energy has been key to many developments throughout the history of mankind. Resilience, absent hysteresis, has been an elusive goal to achieve, particularly at large deformations. Using a low-crosslink-density polyacrylamide hydrogel at 96% water content having hyperbranched silica nanoparticles (HBSPs) as the major junction points, a hysteresis-free material is realized. The fatigue-free characteristic of these composite hydrogels is evidenced by the invariance of the stress-strain curves at strain ratios of 4, even after 5000 cycles. At a strain ratio of 7, only a 1.3% hysteresis is observed. A markedly increased strain-ratio-at-break of 11.5 is observed. The unique attributes of these resilient hydrogels are manifested in the high-fidelity detection of dynamic deformations under cyclic loading over a broad range of frequencies, difficult to achieve with other materials.
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Cellulose-dissolving ionic liquids (ILs) have been used in biomass pretreatment for over a decade. Cellulose solubility in the ILs is strongly inhibited by water, which has negative impacts on IL pretreatment and reuse of the recycled ILs. Here, a distillation and aeration apparatus was used as the reactor for biomass pretreatment in dilute aqueous IL solutions and in recycled IL liquor without drying or purification. Four biomass types, switchgrass, miscanthus, sorghum and pine, were studied. X-ray diffraction (XRD) was used to measure the interaction between biomass and the IL. Small angle neutron scattering (SANS) was applied to monitor the changes of the pore structure in wet biomass samples. Satisfactory enzymatic hydrolysis results were obtained among all the pretreated samples.
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The RAS-RAF-MEK-ERK signaling pathway plays a key role to regulate multiple cellular functions. Acquired resistance to the first-generation RAF inhibitors that only targeted the bRAFV600E mutation prompted the need for a new generation of RAF inhibitors to target cancers bearing mutant RAS and wild type RAF activity by inhibition of paradoxical activation. Starting from the company's previously reported RAF inhibitor 1, extensive drug potency and drug-like properties optimizations led to the discovery of molecule 33 (SHR902275) with greatly improved in vitro potency and solubility. Molecule 33 exhibited good DMPK (Drug Metabolism and Pharmacokinetics) properties, excellent permeability, and outstanding mouse/rat oral PK. It was further evaluated in an in vivo RAS mutant Calu6 xenograft mouse model and demonstrated dose dependent efficacy. To achieve high exposure in a toxicity study, pro-drug 48 was also explored.
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Antineoplásicos/farmacología , Descubrimiento de Drogas , Inhibidores de Proteínas Quinasas/farmacología , Proteínas Proto-Oncogénicas B-raf/antagonistas & inhibidores , Animales , Antineoplásicos/síntesis química , Antineoplásicos/química , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Femenino , Humanos , Ratones , Ratones Desnudos , Modelos Moleculares , Estructura Molecular , Neoplasias Experimentales/tratamiento farmacológico , Neoplasias Experimentales/metabolismo , Neoplasias Experimentales/patología , Inhibidores de Proteínas Quinasas/síntesis química , Inhibidores de Proteínas Quinasas/química , Proteínas Proto-Oncogénicas B-raf/metabolismo , Ratas , Ratas Sprague-Dawley , Proteínas Recombinantes/metabolismo , Relación Estructura-ActividadRESUMEN
Aggregation-structure formation of conjugated polymers is a fundamental problem in the field of organic electronics and remains poorly understood. Herein, the molar mass dependence of the aggregation structure of a high-mobility conjugated copolymer (TDPP-Se) comprising thiophene-flanked diketopyrrolopyrrole and selenophene is thoroughly shown. Five batches of TDPP-Se are prepared with the number-average molecular weights (Mn ) varied greatly from 21 to 135 kg mol-1 . Small-angle neutron scattering and transmission electron microscopy are combined to probe the solution structure of these polymers, consistently using a deuterated solvent. All the polymers adopt 1D rod-like aggregation structures and the radius of the 1D rods is not sensitive to the Mn , while the length increases monotonically with Mn . By utilizing the ordered packing of the aggregated structure in solution, a highly aligned and ordered film is prepared and, thereafter, a reliable hole mobility of 13.8 cm2 V-1 s-1 is realized in organic thin-film transistors with the moderate Mn batch via bar coating. The hole mobility is among the highest values reported for diketopyrrolopyrrole-based polymers. This work paves the way to visualize the real aggregated structure of polymer semiconductors in solution and sheds light on the microstructure control of high-performance electronic devices.
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Although it is well-known proteins and their complexes are hierarchically organized and highly ordered structures, it remains a major challenge to replicate their hierarchical self-assembly process and to fabricate multihierarchical architectures with well-defined shapes and monodisperse characteristic sizes via peptide self-assembly. Here we describe an amphiphilic short peptide Ac-I3GGHK-NH2 that first preassembles into thin, left-handed ß-sheet nanofibrils, followed by their ordered packing into right-handed nanotubes. The key intermediate morphology and structures featuring the hierarchical process are simultaneously demonstrated. Further mechanistic exploration with the variants Ac-I3GGGK-NH2, Ac-I3GGFK-NH2, and Ac-I3GGDHDK-NH2 reveals the vital role of multiple His-His side chain interactions between nanofibrils in mediating higher-order assembly and architectures. Altogether, our findings not only advance current understanding of hierarchical assembly of peptides and proteins but also afford a paradigm of how to take advantage of side chain interactions to construct higher-order assemblies with enhanced complexities.
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Nanotubos , Péptidos , Interacciones Hidrofóbicas e Hidrofílicas , Nanotubos/química , Péptidos/química , Conformación Proteica en Lámina beta , Estructura Secundaria de ProteínaRESUMEN
With access to the solution structures of nanocomposites of coordination nanocages (CNCs) via scattering and chromatography techniques, their mysterious solution dynamics have been, for the first time, resolved, and interestingly, the surface macromolecules can be substituted by extra free macromolecules in solutions. Obvious exchange of macromolecules can be observed in the solution mixtures of CNC nanocomposites at high temperatures, revising the understanding of the dynamics of CNC nanocomposites. Being distinct from nanocomposites of a simple coordination complex, the quantified solution dynamics of CNC nanocomposites indicates a typical logarithmic time dependence with the dissociation of surface macromolecules as the thermodynamically limiting step, suggesting strongly coupled and hierarchically constrained dynamics among the surface macromolecules. Their dynamics can be activated only upon application of high temperature or selected solvents, and therefore, the rational design of polymer assemblies, for example, hybrid-arm star polymers with precisely controlled compositions and reprocessable, robust CNC-cross-linked supramolecular polymer networks, is facilitated.
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Pterostilbene, a natural bibenzjyl compound, has been demonstrated to have pleiotropic anticancer effects against a variety of cancer types. The aim of this study was carried out to disclose the metabolic profiles of pterostilbene using rat, dog and human hepatocytes. Metabolites were characterized by ultra-high-performance liquid chromatography/quadrupole Orbitrap mass spectrometry with electrospray ionization interface operating in positive ion mode. The structures of the metabolites were proposed by accurate MS, MS/MS spectra and based on their fragmentation patterns. A total of 12 metabolites, including six new ones, were detected and identified. M10 and M12 were unambiguously identified as pinostilbene and 3'-hydroxy-pterostilbene, respectively, by using reference standards. Our results revealed that pterostilbene was metabolized through the following pathways: (a) hydroxylation to form 3'-hydroxy-pterostilbene (M12), which further undergoes glucuronidation (M9), demethylation (M7) and GSH conjugation through the ortho-quinone intermediate; (b) demethylation to produce desmethyl-pterostilbene (M10), which is further subject to glucuronidation (M4); (c) direct conjugation with glucuronide (M11); and (d) direct sulfation (M8). Among the tested species, no significant species difference was observed. The current study provides valuable information on the metabolism of pterostilbene, which is helpful for us to understand the action of this compound.
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Hepatocitos/metabolismo , Estilbenos , Espectrometría de Masas en Tándem/métodos , Animales , Células Cultivadas , Cromatografía Líquida de Alta Presión/métodos , Perros , Humanos , Ratas , Ratas Sprague-Dawley , Estilbenos/análisis , Estilbenos/química , Estilbenos/metabolismoRESUMEN
The rational design and control of electrocatalysts at single-atomic sites could enable unprecedented atomic utilization and catalytic properties, yet it remains challenging in multimetallic alloys. Herein, the first example of isolated Rh atoms on ordered PtBi nanoplates (PtBi-Rh1 ) by atomic galvanic replacement, and their subsequent transformation into a tensile-strained Pt-Rh single-atom alloy (PtBi@PtRh1 ) via electrochemical dealloying are presented. Benefiting from the Rh1 -tailored Pt (110) surface with tensile strain, the PtBi@PtRh1 nanoplates exhibit record-high and all-round superior electrocatalytic performance including activity, selectivity, stability, and anti-poisoning ability toward ethanol oxidation in alkaline electrolytes. Density functional theory calculations reveal the synergism between effective Rh1 and tensile strain in boosting the adsorption of ethanol and key surface intermediates and the CC bond cleavage of the intermediates. The facile synthesis of the tensile-strained single-atom alloy provides a novel strategy to construct model nanostructures, accelerating the development of highly efficient electrocatalysts.
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Notoginsenoside Ft1 (NGFt1), a dammarane triterpene glycoside isolated from Panax notoginseng, showed potent effective in stimulating platelet aggregation in our previous assay, yet its pharmacokinetic behavior is still unclear. This study describes a rapid and sensitive ultra-high-pressure LC-tandem mass spectrometry assay for determining of NGFt1 in rat plasma. Methanol-mediated precipitation was used for sample pre-treatment. Chromatographic separation was achieved on a C18 column with gradient elution using water and acetonitrile as mobile phase. Determination was obtained using an electrospray ionization source in negative selected reaction monitoring (SRM) mode at the transitions of m/z 915.9 â m/z 783.8 and m/z 799.8 â m/z 637.8 for NGFt1 and internal standard, respectively. The assay was linear over the concentration range 0.25-2500 ng/mL (r > 0.995) with the lower limit of quantification of 0.25 ng/mL. The intra- and inter-day precisions (relative standard deviation, %) ranged 1.65%-9.84% and 2.46%-13.49%, respectively, whereas accuracy (relative recovery, %) ranged from 96.21% to 99.45%, respectively. The recovery ranged from 95.09% to 102.22% and the matrix effect from 98.29% to 100.13%. The analyte was stable under tested storage conditions. The method has been successfully applied to a preclinical pharmacokinetic study in rats after a single intravenous (2 mg/kg) and oral (50 mg/kg) administration.
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Cromatografía Líquida de Alta Presión/métodos , Saponinas/sangre , Saponinas/farmacocinética , Espectrometría de Masas en Tándem/métodos , Animales , Femenino , Modelos Lineales , Masculino , Ratas , Reproducibilidad de los Resultados , Saponinas/química , Sensibilidad y EspecificidadRESUMEN
INTRODUCTION: Drug metabolism is one of the most important processes involving a drug after administration. Undesirable pharmacokinetic properties may lead to drug discontinuation. In the past several decades, a number of drugs have been withdrawn from the market due to safety issues caused by metabolites, especially reactive metabolites (RMs). Area covered: The focus of this review is on the role that drug metabolites play in drug discovery and developmental stages, with particular emphasis on metabolism-guided lead optimization, safety assessment of drug metabolites, drug-drug interaction potential of metabolites, and RMs safety assessment. In addition, species-related metabolic differences are briefly covered. Expert opinion: For the safety assessment of drug metabolites, a number of factors should be given full consideration, such as dose, in vitro and in vivo correlations, in vivo animal toxicological findings, and the accumulation of metabolites in plasma and/or tissues. Several factors, especially dose and multiple metabolic pathways, can significantly affect the occurrence of idiosyncratic adverse drug reactions (IADRs). Multiple assays should be used to assess RMs and thus avoid false-negative results. There is no clear interplay between the formation of RMs and the occurrence of IADRs. Avoidance of structural alerts and decreasing dose are the most effective strategies in reducing the risk of IADRs.