Mechanisms insights into Cd passivation in soil by lignin biochar: Transition from flooding to natural air-drying.

Biochar shows great potential in soil cadmium pollution treatment, however, the effect and mechanisms of biochar on cadmium passivation (CP) during the long-term process of soil from flooding to natural air-drying are not clear. In this study, a 300-day experiment was conducted to keep the flooded water level constant for the first 100 days and then dried naturally. Mechanisms of CP by lignin biochar (LBC) were analyzed through chemical analysis, FTIR-2D-COS, EEMs-PARAFAC, ultraviolet spectroscopy characterizations, and microbial community distribution of soil. Results showed that application of LBC results in rapid CP ratio in soil within 35 days, mainly in the residual and Fe-Mn bound states (total 72.80%). CP ratio further increased to 90.89% with water evaporation. The CP mechanisms include precipitation, electrostatic effect, humus complexation, and microbial remediation by promoting the propagation of fungi such as Penicillium and Trichoderma. Evaporation of water promoted the colonization of aerobic microorganisms and then increased the degree of soil humification and aromatization, thereby enhancing the cadmium passivation. Simultaneously, the biochar could reduce the relative abundance of plant pathogens in soil from 1.8% to 0.03% and the freshness index (ß/α) from 0.64 to 0.16, favoring crop growth and promoting carbon sequestration and emission reduction.

Efficient electrosynthesis of HO2- from air for sulfide control in sewers.

Electrochemically in-situ generation of oxygen and caustic soda is promising for sulfide management while suffers from scaling, poor inactivating capacity, hydrogen release and ammonia escape. In this study, the four-compartment electrochemical cell efficiently captured oxygen molecules from the air chamber to produce HO2- without generating toxic by-products. Meanwhile, the catalyst layer surface of PTFE/CB-GDE maintained a relatively balanced gas-liquid micro-environment, enabling the formation of enduring solid-liquid-gas interfaces for efficient HO2- electrosynthesis. A dramatic increase in HO2- generation rate from 453.3 mg L-1 h-1 to 575.4 mg L-1 h-1 was attained by advancement in operation parameters design (flow channels, electrolyte types, flow rates and circulation types). Stability testing resulted in the HO2- generation rate over 15 g L-1 and the current efficiency (CE) exceeding 85%, indicating a robust stable operational capacity. Furthermore, after 120 mg L-1 HO2- treatment, an increase of 11.1% in necrotic and apoptotic cells in the sewer biofilm was observed, higher than that achieved with the addition of NaOH, H2O2 method. The in-situ electrosynthesis strategy for HO2- represents a significance toward the practical implementation of sulfide abatement in sewers, holding the potential to treat various sulfide-containing wastewater.

Recent trends in the delivery of RNA drugs: Beyond the liver, more than vaccine.

RNAs are known for versatile functions and therapeutic utility. They have gained significant interest since the approval of several RNA drugs, including COVID-19 mRNA vaccines and therapeutic agents targeting liver diseases. There are increasing expectations for a new class of RNA drugs for broader applications. Successful development of RNA drugs for new applications hinges on understanding their diverse functions and structures. In this review, we explore the last five years of literature to understand current approaches to formulate a spectrum of RNA drugs, focusing on new efforts to expand their applications beyond vaccines and liver diseases.

BmPGPR-L4 is a negative regulator of the humoral immune response in the silkworm Bombyx mori.

Toll, immune deficiency and prophenoloxidase cascade represent vital immune signaling pathways in insects. Peptidoglycan recognition proteins (PGRPs) are innate immune receptors that activate and regulate the immune signaling pathways. Previously, we reported that BmPGPR-L4 was induced in the silkworm Bombyx mori larvae by bacteria and peptidoglycan challenges. Here, we focused on the function of BmPGRP-L4 in regulating the expression of antimicrobial peptides (AMPs). The hemolymph from BmPGRP-L4-silenced larvae exhibited an enhanced inhibitory effect on the growth of Escherichia coli, either by growth curve or inhibitory zone experiments. Coincidentally, most of the AMP genes were upregulated after RNAi of BmPGRP-L4. Oral administration of heat-inactivated E. coli and Staphylococcus aureus after RNAi of BmPGRP-L4 resulted in the increased expression of BmPGRP-L4 in different tissues of the silkworm larvae, revealing an auto-regulatory mechanism. By contrast, the expression of most AMP genes was downregulated by oral bacterial administration after RNAi of BmPGRP-L4. The above results demonstrate that BmPGRP-L4 recognizes bacterial pathogen-associated molecular patterns and negatively regulates AMP expression to achieve immunological homeostasis. As a negative regulator, BmPGPR-L4 is proposed to be involved in the feedback regulation of the immune signaling pathways of the silkworm to prevent excessive activation of the immune response.

Sulfur-containing substances in sewers: Transformation, transportation, and remediation.

Sulfur-containing substances in sewers frequently incur unpleasant odors, corrosion-related economic loss, and potential human health concerns. These observations are principally attributed to microbial reactions, particularly the involvement of sulfate-reducing bacteria (SRB) in sulfur reduction process. As a multivalent element, sulfur engages in complex bioreactions in both aerobic and anaerobic environments. Organic sulfides are also present in sewage, and these compounds possess the potential to undergo transformation and volatilization. In this paper, a comprehensive review was conducted on the present status regarding sulfur transformation, transportation, and remediation in sewers, including both inorganic and organic sulfur components. The review extensively addressed reactions occurring in the liquid and gas phase, as well as examined detection methods for various types of sulfur compounds and factors affecting sulfur transformation. Current remediation measures based on corresponding mechanisms were presented. Additionally, the impacts of measures implemented in sewers on the subsequent wastewater treatment plants were also discussed, aiming to attain better management of the entire wastewater system. Finally, challenges and prospects related to the issue of sulfur-containing substances in sewers were proposed to facilitate improved management and development of the urban water system.

Polyvinyl Chloride Microplastics Facilitate Nitrous Oxide Production in Partial Nitritation Systems.

Partial nitritation (PN) is an important partner with anammox in the sidestream line treating high-strength wastewater and primarily contributes to nitrous oxide (N2O) emissions in such a hybrid system, which also suffers from ubiquitous microplastics because of the growing usage and disposal levels of plastics. In this study, the influences of polyvinyl chloride microplastics (PVC-MPs) on N2O-contributing pathways were experimentally revealed to fill the knowledge gap on N2O emission from the PN system under microplastics stress. The long-term results showed that the overall PN performance was hardly affected by the low-dose PVC-MPs (0.5 mg/L) while obviously deteriorated by the high dose (5 mg/L). According to the batch tests, PVC-MPs reduced biomass-specific ammonia oxidation rates (AORs) by 5.78-21.94% and stimulated aerobic N2O production by 9.22-88.36%. Further, upon increasing dissolved oxygen concentrations from 0.3 to 0.9 mg O2/L, the degree of AOR inhibition increased but that of N2O stimulation was lightened. Site preference analysis in combination with metabolic inhibitors demonstrated that the contributions of hydroxylamine oxidation and heterotrophic denitrification to N2O production at 0.3 mg O2/L were enhanced by 18.84 and 10.34%, respectively, accompanied by a corresponding decreased contribution of nitrifier denitrification. Finally, the underlying mechanisms proposed for negative influences of PVC-MPs were bisphenol A leaching and reactive oxygen species production, which led to more cell death, altered sludge properties, and reshaped microbial communities, further resulting in enhanced N2O emission. Overall, this work implied that the ubiquitous microplastics are a hidden danger that cannot be ignored in the PN system.

Highly efficient Ni(II) adsorption by industrial lignin-based biochar: a pivotal role of dissolved substances within biochar.

In the context of carbon neutrality, promoting resource utilization of industrial alkali lignin addressing heavy metal pollution is crucial for China's pollution alleviation and carbon reduction. Microwave pyrolysis produced functionalized biochar from industrial alkali lignin for Ni(II) adsorption. LB400 achieved 343.15 mg g-1 saturated adsorption capacity in 30 min. Pseudo-second-order kinetic and Temkin isotherm models accurately described the adsorption, which was endothermic and spontaneous (ΔGÏ´ < 0, ΔHÏ´ > 0). Quantitative analysis revealed that both dissolved substances and carbon skeleton from biochar contributed to adsorption, with the former predominates (93.76%), including mineral precipitation NiCO3 (Qp) and adsorption of dissolved organic matter (QDOM). Surface complexation (Qc) and ion exchange (Qi) on the carbon skeleton accounted for 6.3%. Higher biochar preparation temperature reduced Ni(II) adsorption by dissolved substances. Overall, biochar which comes from the advantageous disposal of industrial lignin effectively removes Ni(II) contamination, encouraging ecologically sound treatment of heavy metal pollution and sustainable resource utilization.

Smaller Aerobic Granules Significantly Reduce N2O Production by Ammonia-Oxidizing Bacteria: Evidences from Biochemical and Isotopic Analyses.

The mitigation of nitrous oxide (N2O) is of primary significance to offset carbon footprints in aerobic granular sludge (AGS) systems. However, a significant knowledge gap still exists regarding the N2O production mechanism and its pathway contribution. To address this issue, the impact of varying granule sizes, dissolved oxygen (DO), and nitrite (NO2-) levels on N2O production by ammonia-oxidizing bacteria (AOB) during nitrification in AGS systems was comprehensively investigated. Biochemical and isotopic experiments revealed that increasing DO or decreasing NO2- levels reduced N2O emission factors (by 13.8 or 19.5%) and production rates (by 0.08 or 0.35 mg/g VSS/h) via weakening the role of the AOB denitrification pathway since increasing DO competed for more electrons required for AOB denitrification. Smaller granules (0.5 mm) preferred to diminish N2O production via enhancing the role of NH2OH pathway (i.e., 59.4-100% in the absence of NO2-), while larger granules (2.0 mm) induced conspicuously higher N2O production via the AOB denitrification pathway (approximately 100% at higher NO2- levels). Nitrifying AGS systems with a unified size of 0.5 mm achieved 42% N2O footprint reduction compared with the system with mixed sizes (0.5-2.0 mm) under optimal conditions (DO = 3.0 mg-O2/L and NO2- = 0 mg-N/L).

Mitigating greenhouse gas emissions from municipal wastewater treatment in China.

Municipal wastewater treatment plays an indispensable role in enhancing water quality by eliminating contaminants. While the process is vital, its environmental footprint, especially in terms of greenhouse gas (GHG) emissions, remains underexplored. Here we offer a comprehensive assessment of GHG emissions from wastewater treatment plants (WWTPs) across China. Our analyses reveal an estimated 1.54 (0.92-2.65) × 104 Gg release of GHGs (CO2-eq) in 2020, with a dominant contribution from N2O emissions and electricity consumption. We can foresee a 60-65% reduction potential in GHG emissions with promising advancements in wastewater treatment, such as cutting-edge biological techniques, intelligent wastewater strategies, and a shift towards renewable energy sources.

Unveiling the roles of biofilm in reducing N2O emission in a nitrifying integrated fixed-film activated sludge (IFAS) system.

Biofilm process such as integrated fixed-film activated sludge (IFAS) system has been preliminarily found to produce less nitrous oxide (N2O) than suspended sludge system. However, the N2O emission behaviors and underlying N2O mitigation mechanism in such hybrid system remain unclear. This study therefore aims to fully unveil the roles of biofilm in reducing N2O emission in a nitrifying IFAS system with the aid of some advanced technologies such as N2O microsensor and site-preference analysis. It was found that ammonia oxidation occurred mostly in the sludge flocs (˃ 86%) and biofilm could reduce N2O emission by 43.77% in a typical operating cycle. Biofilm not only reduced nitrite accumulation in nitrification process, inhibiting N2O production via nitrifier denitrification pathway, but also served as a N2O sink, promoting the reduction of N2O via endogenous denitrification. As a result, N2O emissions from the IFAS system were 50%-83% lower than those from the solo sludge flocs. Further, more N2O emission was reduced in the presence of biofilm with decreasing the dissolved oxygen level in the range of 0.5-3.0 mg O2/L. Microbial community and key enzyme analyses revealed that biofilm had relatively high microbial diversity and unique enzyme composition, providing a reasonable explanation for the changed contributions of different N2O production pathways and reduced N2O emission.

Competition between p53 and YY1 determines PHGDH expression and malignancy in bladder cancer.

PURPOSE: Serine metabolism is frequently dysregulated in many types of cancers and the tumor suppressor p53 is recently emerging as a key regulator of serine metabolism. However, the detailed mechanism remains unknown. Here, we investigate the role and underlying mechanisms of how p53 regulates the serine synthesis pathway (SSP) in bladder cancer (BLCA). METHODS: Two BLCA cell lines RT-4 (WT p53) and RT-112 (p53 R248Q) were manipulated by applying CRISPR/Cas9 to examine metabolic differences under WT and mutant p53 status. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) and non-targeted metabolomics analysis were adopted to identify metabolomes changes between WT and p53 mutant BLCA cells. Bioinformatics analysis using the cancer genome atlas and Gene Expression Omnibus datasets and immunohistochemistry (IHC) staining was used to investigate PHGDH expression. Loss-of-function of PHGDH and subcutaneous xenograft model was adopted to investigate the function of PHGDH in mice BLCA. Chromatin immunoprecipitation (Ch-IP) assay was performed to analyze the relationships between YY1, p53, SIRT1 and PHGDH expression. RESULTS: SSP is one of the most prominent dysregulated metabolic pathways by comparing the metabolomes changes between wild-type (WT) p53 and mutant p53 of BLCA cells. TP53 gene mutation shows a positive correlation with PHGDH expression in TCGA-BLCA database. PHGDH depletion disturbs the reactive oxygen species homeostasis and attenuates the xenograft growth in the mouse model. Further, we demonstrate WT p53 inhibits PHGDH expression by recruiting SIRT1 to the PHGDH promoter. Interestingly, the DNA binding motifs of YY1 and p53 in the PHGDH promoter are partially overlapped which causes competition between the two transcription factors. This competitive regulation of PHGDH is functionally linked to the xenograft growth in mice. CONCLUSION: YY1 drives PHGDH expression in the context of mutant p53 and promotes bladder tumorigenesis, which preliminarily explains the relationship between high-frequency mutations of p53 and dysfunctional serine metabolism in bladder cancer.

Evaluation of nitrogen removal and nitrous oxide turnovers in granule-based simultaneous nitrification and denitrification system.

Nitrous oxide (N2O) is an inevitable intermediate generated during the nitrogen removal process of granule-based simultaneous nitrification and denitrification (SND) system. In order to alleviate N2O production while maintaining a desired total nitrogen (TN) removal level in this system, a comprehensive evaluation of the contribution pathways and process parameters affecting N2O turnovers is keenly required. Therefore, mathematical models were applied to evaluate the impact of operating conditions and unravel potential mechanisms on TN removal performance and N2O production. Simulation results show that higher N2O production (11.6 %-14.2 %) occurs at higher dissolved oxygen (DO) concentrations, lower chemical oxygen demand (COD) levels, longer hydraulic retention time (HRT) and larger granule size in the granular SND system. The relative conversion rates of nitrogenous components in different regions within the granule influence N2O turnovers, with the nitrification process occurring only in the region 200 µm inward from the granule surface and denitrification working throughout the entire granule. In the inner region of the granule (0-300 µm), the heterotrophic bacteria (HB) denitrification pathway dominates N2O production as a source of N2O. While in the outer region (300-450 µm), HB denitrification acts as a sink for N2O and regulates N2O turnovers (i.e. production and reduction of N2O) together with the hydroxylamine (NH2OH) pathway that is the main contributor of N2O production. Moreover, simultaneous adjustment of multiple operating parameters within a certain range can lower the N2O production factor (<0.5 %) while achieving the desired TN removal efficiency (>80 %), resulting in a feasible N2O mitigation strategy.

Protein corona: Friend or foe? Co-opting serum proteins for nanoparticle delivery.

For systemically delivered nanoparticles to reach target tissues, they must first circulate long enough to reach the target and extravasate there. A challenge is that the particles end up engaging with serum proteins and undergo immune cell recognition and premature clearance. The serum protein binding, also known as protein corona formation, is difficult to prevent, even with artificial protection via "stealth" coating. Protein corona may be problematic as it can interfere with the interaction of targeting ligands with tissue-specific receptors and abrogate the so-called active targeting process, hence, the efficiency of drug delivery. However, recent studies show that serum protein binding to circulating nanoparticles may be actively exploited to enhance their downstream delivery. This review summarizes known issues of protein corona and traditional strategies to control the corona, such as avoiding or overriding its formation, as well as emerging efforts to enhance drug delivery to target organs via nanoparticles. It concludes with a discussion of prevailing challenges in exploiting protein corona for nanoparticle development.

Insights into N2O turnovers under polyethylene terephthalate microplastics stress in mainstream biological nitrogen removal process.

The ubiquitous microplastics in wastewater have raised growing concerns due to their unintended effects on microbial activities. However, whether and how microplastics affect nitrous oxide (N2O) (a potent greenhouse gas) turnovers in mainstream biological nitrogen removal (BNR) process remain unclear. This work therefore aimed to fill such knowledge gap by conducting both long-term and batch tests. After over 100 days of feeding with wastewater containing polyethylene terephthalate (PET) microplastics (0-500 µg/L), the long-term results showed that both production and reduction of N2O during denitrification were reduced, as well as the N2O production during nitrification. Accordingly, 60% reduction in N2O accumulation and 70% reduction in N2O production were observed in the denitrification and nitrification batch tests, respectively. Nevertheless, the long-term N2O emission factors under PET microplastics stress were comparable to that in the control reactor, mainly because PET microplastics led to more nitrite accumulation in anoxic period. With the aid of online N2O sensors and site-preference analysis, it was demonstrated that the heterotrophic bacteria pathway and ammonia oxidizing bacteria denitrification pathway for N2O production were negatively affected by PET microplastics, whereas a clear increase in the contribution of hydroxylamine pathway (+ 22.9%) was observed. Further investigation revealed that PET microplastics even at environmental level (i.e. 10 µg/L) significantly reshaped the BNR sludge characteristics (e.g. much larger particle size) and microbial communities (e.g. Thauera, Rhodobacte and Nitrospira) as well as the nitrogen metabolism pathways, which were chiefly responsible for the changes of N2O turnovers and N2O production pathways under the PET microplastics stress.

A single local delivery of paclitaxel and nucleic acids via an immunoactive polymer eliminates tumors and induces antitumor immunity.

Despite recent advances in cancer therapy, hard-to-reach, unidentified tumors remain a significant clinical challenge. A promising approach is to treat locatable and accessible tumors locally and stimulate antitumor immunity in situ to exert systemic effects against distant tumors. We hypothesize that a carrier of immunotherapeutics can play a critical role in activating antitumor immunity as an immunoadjuvant and a local retainer of drug combinations. Here, we develop a polyethyleneimine-lithocholic acid conjugate (2E'), which forms a hydrophobic core and cationic surface to codeliver hydrophobic small molecules and anionic nucleic acids and activates antigen-presenting cells via the intrinsic activities of 2E' components. 2E' delivers paclitaxel and small-interfering RNA (siRNA) targeting PD-L1 (or cyclic dinucleotide, [CDN]) to induce the immunogenic death of tumor cells and maintain the immunoactive tumor microenvironment, and further activates dendritic cells and macrophages, leveraging the activities of loaded drugs. A single local administration of 2E' or its combination with paclitaxel and PD-L1­targeting siRNA or CDN induces strong antitumor immunity, resulting in immediate regression of large established tumors, tumor-free survival, an abscopal effect on distant tumors, and resistance to rechallenge and metastasis in multiple models of murine tumors, including CT26 colon carcinoma, B16F10 melanoma, and 4T1 breast cancer. This study supports the finding that local administration of immunotherapeutics, when accompanied by the rationally designed carrier, can effectively protect the host from distant and recurrent diseases.

ZIF-8-derived photocatalyst membrane for water decontamination: From static adsorption-degradation to dynamic flow removal.

Photocatalysis has been considered a promising method for environmental purification. However, powder nanomaterials are not suitable for large-scale application due to the limit of low recyclability and energy-intensive operation. Integrating and depositing powder photocatalysts on monolithic substrates may solve these issues. In this study, a ZIF-8 photocatalyst membrane and its derived product (ZnS photocatalyst membrane) was constructed by a facile in-situ treatment of cellulose-based substrate (take filter paper as an example). Both the two nanomaterials were confirmed to be tightly anchored to filter paper with the aid of chemical interaction. Under visible light irradiation, excellent dynamic-flow photocatalytic removal efficiencies of methylene blue (MB) degradation (97% within 80 min, k = 0.042 ± 0.002 min-1) and Cr(VI) reduction (100% within 60 min, k = 0.116 ± 0.007 min-1) were achieved by the prepared ZIF-8 photocatalyst membrane and its derived ZnS photocatalyst, respectively. Considering the high MB adsorption capacity and facile regeneration process of ZIF-8 photocatalyst membrane, the adsorption-degradation strategy was promising for its universal applications. The MB degradation pathway and photocatalytic mechanisms were also explored. Ultimately, a comprehensive discussion on the advantages and implications of prepared photocatalyst membranes for photocatalytic water treatment was rationally proposed. This study provided a promising method for water decontamination and demonstrated the significant superiority of monolithic membrane for photocatalytic water treatment.

One-pot synthesis of oxygen-vacancy-rich Cu-doped UiO-66 for collaborative adsorption and photocatalytic degradation of ciprofloxacin.

UiO-66, as one of the most stable metal-organic frameworks (MOFs), has attracted a lot of attention in the field of adsorption and photocatalysis. However, this application of UiO-66 is still limited due to either the low accessibility of micropores or the poor electron-hole charge separation capability. This study aims to promote UiO-66 accessibility of micropores and charge separation through the construction of oxygen vacancies (OVs) and mesopore defects as well as copper incorporation. Herein, mesopore Cu doped UiO-66 with rich OVs was synthesized by a one-pot method and demonstrated high efficiency for the removal of ciprofloxacin (CIP) from the aquatic system. First of all, denatured mesopore defects were produced in Cu doped UiO-66 which possessed a 58% increase in specific surface area compared to UiO-66, facilitating the adsorption of molecular oxygen. Secondly, e- was preferentially trapped by OVs under light irradiation. Electron (e-) reacted rapidly with the surface adsorbed oxygen to generate superoxide radical (O2-). Meanwhile, copper incorporation increased the photocurrent and reduced the interfacial charge transfer resistance, thereby improving the charge separation efficiency. As a result, the adsorption efficiency and photocatalytic performance of mesopore Cu doped UiO-66 with OVs were 8.1 and 3.7 times higher than those of UiO-66, respectively. This study paved a way for the one-step synthesis of MOFs containing OVs and broadened the possibilities of practical applications for photo-induced removal of antibiotics from effluent.

Photocatalytic degradation of tetracycline by metal-organic frameworks modified with Bi2WO6 nanosheet under direct sunlight.

Porous metal-organic frameworks (MOFs) with visible-light response have attracted much attention in the field of environmental purification and solar energy conversion. In this study, MIL-100(Fe) was modified with Bi2WO6 nanosheets by a facile hydrothermal method to fabricate a photocatalyst with direct Z-scheme heterojunction. When treating the tetracycline (TC) solution under natural sunlight, 12 wt%MIL-100(Fe)/Bi2WO6 obtained the highest apparent rate constant of (6.59 ± 0.52)✕10-3 L mg-1 min-1, which was 16.1 and 3.9 times than that of pristine MIL-100(Fe) and Bi2WO6, respectively. In addition to explore the feasibility of sunlight-activated MIL-100(Fe)/Bi2WO6 to remove TC under various conditions, the degradation intermediates and their possible transformation pathway were provided with the aid of three-dimensional excitation-emission matrix spectra and liquid chromatography-mass spectrometry system. The results of Escherichia coli culture demonstrated that the biotoxicity variation of TC solution would first increase and then decrease with the photodegradation time. Ultimately, based on the results of bandgap calculation, radicals trapping and charge flow tracking experiments, the direct Z-scheme heterojunction between MIL-100(Fe) and Bi2WO6 nanosheets was confirmed and the photocatalytic mechanism for TC degradation was rationally proposed. This work enriched MOFs-based heterojunction photocatalysts and provided a promising method to eliminate hazardous TC from aqueous solution.

Modified MIL-100(Fe) for enhanced photocatalytic degradation of tetracycline under visible-light irradiation.

Iron-based metal-organic frameworks (MOFs) with low cost and excellent photocatalytic potential are extremely attractive in the field of energy utilization and environmental remediation. In this study, a novel In2S3/MIL-100(Fe) photocatalyst was successfully synthesized by a facile solvothermal method for the first time. Several technologies (such as X-ray diffraction, scanning electron microscope, transmission electron microscope, and X-ray photoelectron spectroscopy) were used to characterize the as-obtained samples and demonstrate the successful combination of MIL-100(Fe) and In2S3. Experimental results showed that 18% of tetracycline (TC) was adsorbed under dark condition and another 70% of TC was degraded under visible-light irradiation when treating 100 mL of TC solution (10 mg/L) with 30 mg of In2S3/MIL-100(Fe) composites. The corresponding TC removal efficiency was almost 1.9 and 1.6 times higher than that of pure MIL-100(Fe) and In2S3, respectively. The mechanism investigations revealed that the heterojunction composite exhibited superior charge transfer than either MIL-100(Fe) or In2S3, and this caused more efficient separation of electron-hole pairs. As a result, more radicals and holes were generated in the composite, leading to better photocatalytic performance. This work highlights the powerful combination of MOFs and semiconductor, which is a promising approach to fabricate heterojunction photocatalyst for wastewater purification.

Discovery of 2-((4,6-dimethylpyrimidin-2-yl)thio)-N-phenylacetamide derivatives as new potent and selective human sirtuin 2 inhibitors.

Human sirtuin 2 (SIRT2) plays pivotal roles in multiple biological processes such as cell cycle regulation, autophagy, immune and inflammatory responses. Dysregulation of SIRT2 was considered as a main aspect contributing to several human diseases, including cancer. Development of new potent and selective SIRT2 inhibitors is currently desirable, which may provide a new strategy for treatment of related diseases. Herein, a structure-based optimization approach led to new 2-((4,6-dimethylpyrimidin-2-yl)thio)-N-phenylacetamide derivatives as SIRT2 inhibitors. SAR analyses with new synthesized derivatives revealed a number of new potent SIRT2 inhibitors, among which 28e is the most potent inhibitor with an IC50 value of 42 nM. The selectivity analyses found that 28e has a very good selectivity to SIRT2 over SIRT1 and SIRT3. In cellular assays, 28e showed a potent ability to inhibit human breast cancer cell line MCF-7 and increase the acetylation of α-tubulin in a dose-dependent manner. This study will aid further efforts to develop highly potent and selective SIRT2 inhibitors for the treatment of cancer and other related diseases.