RESUMEN
This study describes the pathomorphological alterations of bovine tuberculosis through gross and histopathological examinations, assessment of the distribution of lesions and the demonstration of mycobacteria. Samples from lungs, liver, small intestine, their regional lymph nodes and retropharyngeal lymph nodes were collected from 84 cattle with tuberculosis from the Allgäu, Germany. Organs were evaluated grossly, histopathologically and by transmission electron microscopy. Mycobacteria and mycobacterial antigens were demonstrated using acid-fast staining and immunohistochemistry (IHC). Bacteriological tests revealed Mycobacterium caprae in all animals. Gross alterations were classified into five patterns (I to V) with an additional pattern of acute exudative pulmonary inflammation (pattern VI). Histological lesions were classified into four types (1-4) with additional lesions occurring in lungs only. Acid-fast staining revealed a low number of bacteria in all tissues, while IHC showed comparatively more mycobacterial antigens within the lesions and also at their periphery. The alimentary tract (68%) was the main portal of entry followed by an aerosol infection (19%). It was assumed that the observed lesions reflect a continuous primary period of infection; there were no lesions typical of a secondary (post-primary) period, as reported in man and also described in the older literature on bovine tuberculosis. The broad spectrum of changes described formerly was not observed in the present cases and the route of infection and nature of acid fast staining showed differences when compared with previous studies of naturally-occurring bovine tuberculosis.
Asunto(s)
Enfermedades de los Bovinos/patología , Tuberculosis/veterinaria , Animales , Bovinos , Tuberculosis/patologíaRESUMEN
BACKGROUND: Zinc deficiency-like (ZDL) syndrome is an inherited defect of Fleckvieh calves, with striking similarity to bovine hereditary zinc deficiency (BHZD). However, the causative mutation in a phospholipase D4 encoding gene (PLD4) shows no connection to zinc metabolism. OBJECTIVES: To describe clinical signs, laboratory variables, and pathological findings of ZDL syndrome and their utility to differentiate ZDL from BHZD and infectious diseases with similar phenotype. ANIMALS: Nine hospitalized calves with crusting dermatitis and confirmed mutation in PLD4 and medical records from 25 calves with crusting dermatitis or suspected zinc deficiency. METHODS: Prospective and retrospective case series. RESULTS: The 9 calves (age: 5-53 weeks) displayed a moderate to severe crusting dermatitis mainly on the head, ventrum, and joints. Respiratory and digestive tract inflammations were frequently observed. Zinc supplementation did not lead to remission of clinical signs in 4 calves. Laboratory variables revealed slight anemia in 8 calves, hypoalbuminemia in 6 calves, but reduced serum zinc concentrations in only 3 calves. Mucosal erosions/ulcerations were present in 7 calves and thymus atrophy or reduced thymic weights in 8 calves. Histologically, skin lesions were indistinguishable from BHZD. Retrospective analysis of medical records revealed the presence of this phenotype since 1988 and pedigree analysis revealed a common ancestor of several affected calves. CONCLUSIONS AND CLINICAL IMPORTANCE: ZDL syndrome should be suspected in Fleckvieh calves with crusting dermatitis together with diarrhea or respiratory tract inflammations without response to oral zinc supplementation. Definite diagnosis requires molecular genetic confirmation of the PLD4 mutation.
Asunto(s)
Enfermedades de los Bovinos/patología , Zinc/sangre , Animales , Bovinos , Enfermedades de los Bovinos/diagnóstico , Enfermedades de los Bovinos/genética , Dermatitis/diagnóstico , Dermatitis/genética , Dermatitis/veterinaria , Femenino , Masculino , Errores Innatos del Metabolismo de los Metales/diagnóstico , Errores Innatos del Metabolismo de los Metales/genética , Estudios Prospectivos , Estudios Retrospectivos , Síndrome , Zinc/uso terapéuticoRESUMEN
The diagnosis of canine intestinal lymphoma by morphological examination is challenging, especially when endoscopic tissue specimens are used. The utility of detection of antigen receptor gene rearrangement by polymerase chain reaction (PARR) in canine lymphoma has been well established, but its usefulness to distinguish enteritis and intestinal lymphoma remains unclear. In this retrospective study we assessed clonality of 29 primary canine intestinal lymphoma, 14 enteritis and 15 healthy control cases by PARR analysis, using formalin-fixed, paraffin-embedded full-thickness tissue specimens. We could detect monoclonal rearrangements in 22 of 29 canine intestinal lymphomas [76%; 95% confidence interval (CI) 56-90%] and polyclonal rearrangements in all of the enteritis and healthy control cases (100%; CI 88-100%). We revealed a predominance of T-cell phenotype compared to B-cell phenotype (85%; CI 65-96% and 15%; CI 4-35%, respectively). We showed that PARR analysis contributes to differentiation of canine intestinal lymphoma from enteritis and to phenotyping of lymphomas.
Asunto(s)
Enfermedades de los Perros/diagnóstico , Enteritis/veterinaria , Neoplasias Intestinales/veterinaria , Linfoma/veterinaria , Reacción en Cadena de la Polimerasa/veterinaria , Animales , Cartilla de ADN , Diagnóstico Diferencial , Enfermedades de los Perros/genética , Enfermedades de los Perros/patología , Perros , Enteritis/patología , Femenino , Alemania , Inmunoquímica , Neoplasias Intestinales/diagnóstico , Neoplasias Intestinales/genética , Neoplasias Intestinales/patología , Linfoma/diagnóstico , Linfoma/genética , Linfoma/patología , Masculino , Reacción en Cadena de la Polimerasa/métodos , Receptores de Antígenos de Linfocitos B/análisis , Receptores de Antígenos de Linfocitos T/análisis , Estudios RetrospectivosRESUMEN
A 16-year-old Friesian gelding with relapsing colic was humanely destroyed during diagnostic laparotomy due to suspected abdominal neoplasia. On post-mortem examination, the pancreas appeared as a firm mass (20 × 8 × 8 cm). The cut surface had a lobular structure with multiple cavities. Histological examination revealed severe chronic fibrosing pancreatitis with acinar-ductal metaplasia and duct dysplasia, which was considered to be the cause of the recurrent colic. Formation of tubular complexes within a background of acinar-ductal metaplasia is similar to the regressive lesions detected in the human pancreas in the context of inflammation, duct obstruction, cystic fibrosis and neoplasia. Pancreatic acinar-ductal metaplasia and ductal dysplasia are considered to be preneoplastic conditions in man and in the mouse.
Asunto(s)
Enfermedades de los Caballos/patología , Conductos Pancreáticos/patología , Pancreatitis Crónica/veterinaria , Animales , Caballos , Masculino , Metaplasia , Pancreatitis Crónica/patologíaRESUMEN
PCV2 can be divided into three different genotypes: PCV2a, PCV2b and PCV2c. Since 2004/2005 PCV2b has become the predominant genotype in the domestic pig population worldwide. In the years 2010 and 2012 PCV2b mutant strains (mPCV2), classified as PCV2b-1C strains, were detected in porcine circovirus diseases (PCVD) affected pigs in China and the United States, respectively. Within one year (April 2013-April 2014) newly emerging mPCV2 strains were isolated in seven German pig farms routinely vaccinating against PCV2. Histopathological, clinical and molecular biological findings including in-situ hybridization (ISH) and real-time PCR indicate PCVD in the affected animals. Characterized isolates from five farms were closely related to the PCV2b-1C reference strain BDH (GenBank no. HM038017), whereas strains from two other farms were only 99.1% and 99.0% identical (based on the nucleotide sequence of the complete genome) to mPCV2 strain BDH, respectively.
Asunto(s)
Infecciones por Circoviridae/veterinaria , Circovirus/genética , Enfermedades Transmisibles Emergentes/veterinaria , Sus scrofa , Enfermedades de los Porcinos/epidemiología , Enfermedades de los Porcinos/virología , Animales , Secuencia de Bases , Infecciones por Circoviridae/epidemiología , Enfermedades Transmisibles Emergentes/virología , Genotipo , Alemania/epidemiología , Hibridación in Situ/veterinaria , Datos de Secuencia Molecular , Reacción en Cadena en Tiempo Real de la Polimerasa/veterinaria , Análisis de Secuencia de ADN/veterinaria , PorcinosRESUMEN
Current knowledge on bovine besnoitiosis, caused by the emerging apicomplexan pathogen Besnoitia besnoiti, is still fragmentary. So far, studies dealing with ultrastructural pathology focused mainly on the easily accessible chronic stage, whereas ultrastructural investigations of tachyzoites were confined to in vitro studies. In the study presented here, the ultrastructural pathology of naturally B. besnoiti-infected cattle in the acute and chronic disease stages and experimentally B. besnoiti-infected mice was monitored. Further, the ultrastructure of tachyzoites and bradyzoites was investigated. Skin samples of two adult Limousin cows and one adult Limousin bull naturally infected with B. besnoiti and liver and skin samples of gamma-interferon knockout mice infected with B. besnoiti were examined in semithin sections stained with toluidine blue and safranin and in ultrathin sections contrasted with uranyl acetate and lead citrate. Samples of vessel walls of the bull and nasal mucosa of one cow were examined by scanning electron microscopy. Few tachyzoites-like endozoites were detected for the first time in bovine skin, and large numbers of tachyzoites were detected in murine skin and liver. Within tissue cysts in bovine skin, numerous bradyzoites were observed displaying signs of degeneration. Tachyzoites had apicomplexan endozoite ultrastructure. B. besnoiti tachyzoites and bradyzoites differed in shape and the number of amylopectin granules. Transmission and scanning electron microscopy confirmed the presence of two different cyst wall layers, and the present results on cyst wall ultrastructure were in accordance with those previously obtained by histological sections.
Asunto(s)
Enfermedades de los Bovinos/parasitología , Coccidiosis/veterinaria , Sarcocystidae/ultraestructura , Animales , Bovinos , Enfermedades de los Bovinos/patología , Enfermedad Crónica , Coccidiosis/parasitología , Femenino , Regulación de la Expresión Génica , Interferón gamma/genética , Interferón gamma/metabolismo , Masculino , Ratones , Ratones Noqueados , Microscopía Electrónica de Rastreo , Piel/patologíaRESUMEN
The pathogenesis of bovine besnoitiosis, a disease of increasing concern within Europe, is still incompletely understood. In this study, disease progression after natural infection with the causative apicomplexan Besnoitia besnoiti was monitored in histological skin sections of 5 individual female cattle over time. High-frequency skin sampling of 2 cattle with mild and 2 with severe acute, subacute, and chronic besnoitiosis, as well as from 1 animal during subclinical disease, enabled documentation from the beginning of the disease. Skin sections were stained with hematoxylin and eosin and Giemsa, periodic acid-Schiff reaction, and anti-Besnoitia immunohistochemistry. In all 4 clinically affected animals, tachyzoite-like endozoites could be detected for the first time by immunohistochemistry, and tissue cyst evolution was monitored. Besnoitiosis-associated lesions were not detected in the animal showing the subclinical course. Because of the inconsistency of the nomenclature of Besnoitia tissue cyst layers in the literature, a new nomenclature for B. besnoiti cyst wall layers is proposed: tissue cysts consist of a hypertrophied host cell with enlarged nuclei, an intracytoplasmic parasitophorous vacuole with bradyzoites, a sometimes vacuolated inner cyst wall, and an outer cyst wall in more developed cysts. Inner and outer cyst walls can be readily distinguished by using special stains. In 1 animal, extracystic B. besnoiti zoites were immunohistochemically detected during the chronic stage. At necropsy, the 2 severely affected cows displayed large numbers of B. besnoiti cysts in a variety of tissues, including the corium of the claws, contributing mainly to the development of chronic laminitis in these 2 cases.
Asunto(s)
Enfermedades de los Bovinos/patología , Coccidiosis/veterinaria , Enfermedad Aguda , Animales , Infecciones Asintomáticas , Bovinos , Enfermedades de los Bovinos/parasitología , Enfermedad Crónica , Coccidiosis/patología , Progresión de la Enfermedad , Femenino , Masculino , Sarcocystidae , Piel/patologíaAsunto(s)
Characidae/parasitología , Enfermedades de los Peces/patología , Enfermedades de los Peces/parasitología , Infecciones por Mesomycetozoea/patología , Infecciones por Mesomycetozoea/parasitología , Animales , Mesomycetozoea/genética , Microscopía Electrónica de Rastreo , Microscopía Electrónica de Transmisión , ARN Ribosómico 18S/genética , Piel/patologíaRESUMEN
Pancreatic neuroendocrine tumors of glucagon-producing cells are extremely rare in domestic animals. In this report, we describe for the first time, to our knowledge, the incidental finding of multiple glucagon-producing neuroendocrine tumors of the pancreas of a horse. The animal was euthanized due to severe local infection after tooth extraction. On postmortem examination, multiple white nodules of up to 4 cm in diameter were observed in the pancreas. Histologically, pancreatic nodules had the appearance of neuroendocrine neoplasms with positive immunoreactivity for glucagon, synaptophysin, chromogranin A, and neuron-specific enolase. Electron microscopy revealed numerous electron-dense granules, similar to those observed in normal pancreatic alpha cells, in the neoplastic cells. In addition, the left adrenal gland showed multiple hyperplastic foci and adenomas in the medulla that were identified as pheochromocytomas. Based on the morphologic appearance and immunohistochemical staining pattern of pancreatic nodules, a diagnosis of multiple glucagon-producing neuroendocrine tumors was made.
Asunto(s)
Glucagón/metabolismo , Enfermedades de los Caballos/metabolismo , Enfermedades de los Caballos/patología , Tumores Neuroendocrinos/veterinaria , Neoplasias Pancreáticas/veterinaria , Animales , Caballos , Inmunohistoquímica/veterinaria , Microscopía Electrónica/veterinaria , Tumores Neuroendocrinos/metabolismo , Tumores Neuroendocrinos/patología , Neoplasias Pancreáticas/metabolismo , Neoplasias Pancreáticas/patologíaRESUMEN
BACKGROUND: Etiology of hemorrhagic gastroenteritis (HGE) syndrome in dogs is unknown and histopathologic and microbial investigations have only been performed post mortem. OBJECTIVE: To identify characteristic intra vitam endoscopic and histologic mucosal lesions, as well as bacterial species, within the mucosa of dogs with HGE. ANIMALS: Ten dogs diagnosed with HGE were included. Eleven dogs with gastroduodenoscopy and different intestinal diseases were used as controls for microbial changes. Dogs pretreated with antibiotics or diagnosed with any disease known to cause bloody diarrhea were excluded from the study. METHODS: In this prospective study, gastrointestinal biopsies were collected from 10 dogs with HGE. Endoscopic and histologic changes were assessed according to WSAVA guidelines. Biopsies from the stomach, duodenum, ileum, and colon were investigated by histology and by immunohistochemistry for the presence of Clostridium spp. and parvovirus. The first duodenal biopsy taken with a sterile forceps was submitted for bacterial culture. RESULTS: Acute mucosal lesions were only found in the intestines, not in the stomach. Clostridium spp., identified as Clostridium perfringens in 6/9 cases, were detected on the small intestinal mucosa in all dogs with HGE, either by culture or immunohistopathology. In the control group, C. perfringens could only be cultured in one of 11 dogs. CONCLUSIONS AND CLINICAL IMPORTANCE: The results of this study demonstrate an apparent association between C. perfringens and the occurrence of acute hemorrhagic diarrhea. The term "HGE," which implies the involvement of the stomach, should be renamed as "acute hemorrhagic diarrhea syndrome."
Asunto(s)
Infecciones por Clostridium/veterinaria , Clostridium perfringens/crecimiento & desarrollo , Enfermedades de los Perros/microbiología , Enfermedades de los Perros/patología , Hemorragia Gastrointestinal/veterinaria , Animales , Biopsia/veterinaria , Infecciones por Clostridium/microbiología , Infecciones por Clostridium/patología , Recuento de Colonia Microbiana , Perros , Hemorragia Gastrointestinal/microbiología , Hemorragia Gastrointestinal/patología , Inmunohistoquímica/veterinaria , Mucosa Intestinal/microbiología , Mucosa Intestinal/fisiología , Estudios Prospectivos , Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónRESUMEN
OBJECTIVE: Odontogenic tumours in the dog and cat, as well as in other domestic animals and in man occur rarely and can be difficult to diagnose. In the present study a great number of canine and feline odontogenic tumours were investigated histopathologically and classified to provide an appropriate basis for the evaluation of these tumours. MATERIAL AND METHODS: In a retrospective study of a total of 1390 canine and 317 feline oral tumours from the years 1977 to 2007, the tumours of odontogenic origin were selected, characterized histopathologically and classified according to the current human WHO-classification of odontogenic tumours (2005) and the current veterinary WHO-classification of odontogenic tumours (2003). RESULTS: 18% (n=250) of the canine and 3.2% (n=10) of the feline oral tumours proved to be of odontogenic origin. They could be divided into benign (epithelial, epithelial and ectomesenchymal, ectomesenchymal) and malignant (carcinomas and sarcomas) odontogenic tumours with a total of 12 different entities. The odontogenic fibroma was the most common canine (n=167) and feline (n=4) odontogenic tumour. The second most common odontogenic tumour for canines was ameloblastoma (n=74) and that for felines was ameloblastic fibroma (n=2). Four of the 12 entities could be classified according to both WHO-classifications of odontogenic tumours. Seven and two of the 12 entities could only be classified according to the current human WHO-classification and veterinary WHO-classification, respectively. CONCLUSION AND CLINICAL RELEVANCE: The prognostic evaluation of tumours is of the greatest clinical relevance and calls for an absolutely certain diagnosis. Particularly in the case of the rare and histomorphologically complex odontogenic tumours the current veterinary WHO-classification does not meet this requirement and needs to be revised and extended. The human WHO-classification proved to be more efficient when compared to the veterinary one.
Asunto(s)
Enfermedades de los Gatos/patología , Enfermedades de los Perros/patología , Neoplasias de la Boca/veterinaria , Tumores Odontogénicos/veterinaria , Animales , Enfermedades de los Gatos/clasificación , Gatos , Diagnóstico Diferencial , Enfermedades de los Perros/clasificación , Perros , Neoplasias de la Boca/clasificación , Neoplasias de la Boca/patología , Tumores Odontogénicos/clasificación , Tumores Odontogénicos/patología , Pronóstico , Estudios Retrospectivos , Organización Mundial de la SaludRESUMEN
Intersexuality is a rare congenital abnormality in domestic animals. It is reported in numerous species including the swine, goat, horse, cat, and dog. The present work provides an overview of the variety of intersexual conditions known in different dog breeds. Each case was reclassified based on the described gonadal constitution, reproductive tract abnormalities and karyogram, and categorised according to the stages normal sex development is undergoing resulting in three main categories: (1) sex chromosome disorders, (2) disorders of gonadal sex development, and (3) disorders of phenotypic sex development. Reclassification disclosed that the current classification scheme and terminology are inconsistently used in literature masking the real occurrence and frequency of various intersex conditions in dogs. For establishment of an individual, precise and definite diagnosis, introduction of a new nomenclature is proposed as recently recommended for humans. The new terminology is based on the gonosomal constellation and gonadal constitution, contributes to a systematic classification of canine intersex cases, and replaces the common but confusing diagnoses "true hermaphrodite" and "pseudohermaphrodite". The literature survey was supplemented by adding the results from own investigations in a German Pinscher and Berger Picard dog with bilateral ovotestes and ambiguous external genitalia. The diagnostic approach and clinical, pathomorphological and cytogenetic findings were described in detail.
Asunto(s)
Trastornos del Desarrollo Sexual/veterinaria , Enfermedades de los Perros/clasificación , Terminología como Asunto , Animales , Trastornos del Desarrollo Sexual/clasificación , Perros , Femenino , Masculino , Ovario , Fenotipo , Trastornos de los Cromosomas Sexuales/clasificación , Trastornos de los Cromosomas Sexuales/veterinaria , Maduración Sexual , TestículoRESUMEN
This report describes an uncommon case of cryptococcosis in an apparently immunocompetent cat caused by Cryptococcus magnus. An amputation of the complete left foreleg and excision of the ipsilateral cervical lymph node were performed in a young-adult male Domestic Shorthair cat due to suspicion of a tumor. Granulomatous dermatitis, panniculitis, myositis, and lymphadenitis were diagnosed histologically. Intralesional, numerous round-to-ovoid yeast cells showing no capsule were detected within macrophages using special staining methods. The tissue material cultured on Sabouraud's glucose agar at 26°C yielded abundant growth of yeast colonies. Morphological, physiological, and molecular analyses of the yeasts demonstrated that the fungus was C. magnus. Response to treatment with fluconazole was fast and effective, and one year after the end of the therapy no further clinical signs of infection were observed.
Asunto(s)
Enfermedades de los Gatos/microbiología , Criptococosis/veterinaria , Cryptococcus/aislamiento & purificación , Animales , Antifúngicos/uso terapéutico , Secuencia de Bases , Enfermedades de los Gatos/tratamiento farmacológico , Enfermedades de los Gatos/patología , Gatos , Criptococosis/tratamiento farmacológico , Criptococosis/microbiología , Criptococosis/patología , Cryptococcus/genética , ADN Intergénico/genética , Fluconazol/uso terapéutico , Masculino , Datos de Secuencia Molecular , ARN de Hongos/genética , ARN Ribosómico/genéticaRESUMEN
Intestinal obstruction resulting from an intramural foreign body-associated pyogranuloma was diagnosed in four dogs. Vomiting and weight loss were the main clinical signs. On physical examination, a mass in the abdomen was detected in three dogs. Abdominal radiography demonstrated the presence of soft tissue opacity in three of the dogs and gas-filled dilated intestinal loops in all four dogs. Abdominal ultrasonography showed hyperkinetic fluid-filled dilated intestinal loops and a hypoechoic small intestinal mass in all the dogs. Exploratory coeliotomy confirmed the presence of a jejunal mass, which was removed by resection and anastomosis in all the dogs. In one of the dogs a linear foreign body was also found cranial to the mass and was removed through a separate enterotomy incision. The lesions were diagnosed as foreign body-associated intestinal pyogranulomas on histological examination. Three dogs recovered without complications, but the fourth showed signs of septic peritonitis four days after surgery and was euthanased at the owner's request. The other three dogs remained disease-free 12 to 42 months after surgery.
Asunto(s)
Enfermedades de los Perros/diagnóstico , Granuloma de Cuerpo Extraño/veterinaria , Enfermedades Intestinales/veterinaria , Obstrucción Intestinal/veterinaria , Animales , Enfermedades de los Perros/cirugía , Perros , Resultado Fatal , Femenino , Granuloma de Cuerpo Extraño/complicaciones , Granuloma de Cuerpo Extraño/patología , Enfermedades Intestinales/patología , Obstrucción Intestinal/diagnóstico , Obstrucción Intestinal/etiología , Masculino , Radiografía Abdominal/veterinaria , Ultrasonografía/veterinariaRESUMEN
Post-mortem examination was performed on a horse that died after exhibiting signs of colic. Gross findings included haemoperitoneum and a large round encapsulated mass located in the sublumbar area cranial to the left kidney. On sectioning the mass was solid red to brown and small nodules of similar tissue were noted at the periphery of the mass. The spleen was firm and three nodules were found in one thyroid gland. Microscopically, the abdominal mass, adjacent nodules, the spleen and one thyroid nodule consisted of clusters and cords of round to oval neoplastic cells, separated by a fine collagen and reticulin fibre network. Immunohistochemically, tumour cells expressed chromogranin A, synaptophysin and neuron-specific enolase, but did not express cytokeratin. The findings were consistent with a metastatic extra-adrenal sympathetic paraganglioma.
Asunto(s)
Enfermedades del Sistema Nervioso Autónomo/veterinaria , Enfermedades de los Caballos/patología , Paraganglioma Extraadrenal/veterinaria , Neoplasias Peritoneales/veterinaria , Neoplasias de la Tiroides/veterinaria , Animales , Enfermedades del Sistema Nervioso Autónomo/patología , Cromogranina A/metabolismo , Resultado Fatal , Enfermedades de los Caballos/metabolismo , Caballos , Inmunohistoquímica , Masculino , Proteínas Nucleares/metabolismo , Paraganglioma Extraadrenal/metabolismo , Paraganglioma Extraadrenal/secundario , Neoplasias Peritoneales/metabolismo , Neoplasias Peritoneales/secundario , Sinaptofisina/metabolismo , Glándula Tiroides/patología , Neoplasias de la Tiroides/metabolismo , Neoplasias de la Tiroides/patologíaRESUMEN
BACKGROUND: Nonregenerative cytopenias such as nonregenerative anemia, neutropenia, and thrombocytopenia in cats with feline leukemia virus (FeLV) antigen are assumed to be caused by the underlying FeLV infection. In addition, cats with negative FeLV antigen-test results that have cytopenias of unknown etiology often are suspected to suffer from latent FeLV infection that is responsible for the nonregenerative cytopenias. OBJECTIVE: The purpose of this study was to assess the role of latent FeLV infection by polymerase chain reaction (PCR) in bone marrow of cats with nonregenerative cytopenias that had negative FeLV antigen test results in blood. ANIMALS: Thirty-seven cats were included in the patient group. Inclusion criteria were (1) nonregenerative cytopenia of unknown origin and (2) negative FeLV antigen test result. Antigenemia was determined by detection of free FeLV p27 antigen by ELISA in serum. Furthermore, 7 cats with positive antigen test results with nonregenerative cytopenia were included as control group I, and 30 cats with negative antigen test results without nonregenerative cytopenia were included as control group II. METHODS: Whole blood and bone marrow samples were tested by 2 different PCR assays detecting sequences of the envelope or long terminal repeat genes. FeLV immunohistochemistry was performed in bone marrow samples. RESULTS: Two of the 37 cats (5.4%) in the patient group were positive on the bone marrow PCR results and thus were latently infected with FeLV. CONCLUSIONS AND CLINICAL IMPORTANCE: The findings of this study suggest that FeLV latency is rare in cats with nonregenerative cytopenias.
Asunto(s)
Enfermedades de los Gatos/etiología , Enfermedades Hematológicas/veterinaria , Virus de la Leucemia Felina , Infecciones por Retroviridae/veterinaria , Infecciones Tumorales por Virus/veterinaria , Animales , Gatos , Femenino , Enfermedades Hematológicas/complicaciones , Enfermedades Hematológicas/virología , Inmunohistoquímica/veterinaria , Masculino , Reacción en Cadena de la Polimerasa/veterinaria , Infecciones por Retroviridae/complicaciones , Infecciones Tumorales por Virus/complicaciones , Latencia del VirusRESUMEN
Besnoitia besnoiti was in vitro isolated during the first recorded outbreak of bovine besnoitiosis in Germany. Molecular characterization of the new isolate, named Bb-GER1, revealed almost 100% identity with other B. besnoiti isolates obtained in Portugal, Spain, Israel or South Africa, when partial sequences of the 18S ribosomal RNA gene, of the internal transcribed spacer 1 and of the 5.8S RNA gene were compared. Cystozoites obtained from skin tissue of one bull were infectious for gamma-interferon knockout (GKO) mice by intraperitoneal (ip) inoculation. Tachyzoites were detected in the peritoneal cavity, spleen, liver and lung of the mice 5 days post-infection. The parasite could be maintained in GKO mice by ip inoculation for at least 5 passages. Peritoneal washings containing tachyzoites were obtained from infected mice and used to infect five cell lines (Vero, MARC-145, NA42/13, BHK(21), KH-R). The best growth of tachyzoites was observed in BHK(21) cells, but replication occurred to a smaller extent also in MARC-145, NA42/13 and KH-R cells. Subsequent comparative analyses revealed that after direct infection of these cell lines with cystozoites derived from bovine skin, the growth was best in NA42/13 cells. Considerable replication was also observed in the BHK(21) and KH-R cell lines. Our observations on the growth characteristics of Bb-GER1 partially contrast those for other isolates. The preferential growth in particular cell lines may be characteristic for particular B. besnoiti isolates. A potential association between growth properties and differences in virulence remains to be established. This is the first in vitro isolation of B. besnoiti from cattle in Germany.
Asunto(s)
Enfermedades de los Bovinos/parasitología , Coccidiosis/veterinaria , ADN Protozoario/genética , Filogenia , Sarcocystidae/clasificación , Sarcocystidae/aislamiento & purificación , Animales , Bovinos , Enfermedades de los Bovinos/epidemiología , Línea Celular , Coccidiosis/epidemiología , Coccidiosis/parasitología , Cartilla de ADN , ADN Espaciador Ribosómico , Brotes de Enfermedades/veterinaria , Femenino , Alemania/epidemiología , Masculino , Ratones , Ratones Noqueados , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa/veterinaria , ARN Ribosómico 18S/genética , ARN Ribosómico 5.8S/genética , Sarcocystidae/genética , Análisis de Secuencia de ADN , Piel/parasitologíaRESUMEN
A juvenile dwarf rabbit (Oryctolagus cuniculus) with clinical signs of dyspnea and suspected ascites was submitted for necropsy. The main macroscopic findings were a watery red pleural effusion and some whitish striated foci in the lungs. In addition, there were multifocal scars in the cortex of the kidneys. The histologic examination of the lungs showed a severe granulomatous pneumonia with detection of acid-fast bacilli, in the kidneys, an interstitial chronic lymphoplasmacellular nephritis with interstitial fibrosis, and in the brain, a multifocal granulomatous and partly necrotizing encephalitis with detection of spores, suggestive of encephalitozoonosis. In the lungs, Mycobacterium genavense was verified by polymerase chain reaction and 16S ribosomal RNA gene sequencing. To our knowledge, this is the first report of an M. genavense infection in a rabbit, with the lungs being the only affected organ. Therefore, an aerogen infection seems to be the most contemplable way of infection.
Asunto(s)
Infecciones por Mycobacterium/veterinaria , Mycobacterium/inmunología , Neumonía/microbiología , Conejos/microbiología , Animales , ADN Bacteriano/química , ADN Bacteriano/genética , Resultado Fatal , Histocitoquímica/veterinaria , Masculino , Mycobacterium/genética , Infecciones por Mycobacterium/inmunología , Infecciones por Mycobacterium/microbiología , Neumonía/inmunología , ARN Ribosómico 16S/química , ARN Ribosómico 16S/genética , Conejos/inmunología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinariaRESUMEN
Canine angiostrongylosis is a nematode infection in domestic dogs and wild carnivores. Few single case reports describing the occurrence of this disease in Germany exist and until recently angiostrongylosis has not been considered endemic in this country. The present report focuses on clinical, pathological and parasitological findings in two cases of fatal disseminated canine angiostrongylosis associated with multifocal haemorrhages in the central nervous system. Both animals, which lived in Germany, presented with rapidly progressive neurological signs including depression, ataxia, unilateral central blindness and epileptic seizures. Blood work revealed grossly elevated D-dimers and mild thrombocytopenia. Both animals were subsequently euthanised due to progressive clinical aggravation. Necropsy showed cerebral and lung haemorrhages in both animals. Multiple sections of nematode larvae consistent with Angiostrongylus vasorum were identified on histopathological sections of the brain, heart, kidney and lung in both animals and a predominantly granulomatous inflammation with the occurrence of multinucleated giant cells was observed. Adult nematodes were found in the larger lung arteries of one dog and Angiostrongylus infection was subsequently confirmed by PCR-analysis and sequencing in both dogs. A. vasorum larvae were not detected by faecal Baermann examination performed in one of the dogs. It was concluded that canine angiostrongylosis should be considered as differential diagnosis in dogs in Germany, even if faecal examination is negative. There is currently still a lack of studies investigating the occurrence of angiostrongylosis in dogs and intermediate hosts in Germany which would be necessary to survey the endemic realities of this disease.
Asunto(s)
Angiostrongylus , Hemorragia Cerebral/veterinaria , Enfermedades de los Perros/parasitología , Infecciones por Strongylida/veterinaria , Animales , Encéfalo/patología , Hemorragia Cerebral/complicaciones , Hemorragia Cerebral/epidemiología , Hemorragia Cerebral/parasitología , Enfermedades de los Perros/epidemiología , Perros , Resultado Fatal , Femenino , Alemania/epidemiología , Pulmón/patología , Masculino , Infecciones por Strongylida/complicaciones , Infecciones por Strongylida/epidemiología , Infecciones por Strongylida/parasitologíaRESUMEN
In the present study endoscopically diagnosed changes of the Fürstenberg's rosette were examined histologically. For this purpose the teats of 200 slaughtered cows were inspected. In 19 cases proliferations in the region of the Fürstenberg's rosette were found. Additionally, tissue samples were taken from the Fürstenberg's rosette of 26 cows which showed milk flow disorders due to stenoses of this part of the teat. Teats of slaughtered cows as well as biopsy specimens were fixed in 5% formaldehyde and embedded in paraffin and in plastic. All specimens were stained according to H.E., Giemsa and Turnbull, respectively. Histologically, the proliferations of the Fürstenberg's rosette consisted of fibrovascular tissue (granulation tissue) covered by keratinized squamous epithelium (teat canal epithelium) in eleven cases. In the other cases double-layered cuboid epithelium (cisternal epithelium) was found additionally. The formation of granulation tissue and deposition of blood pigment was observed exclusively in areas covered by stratified epithelium. Therefore a traumatic lesion of the teat canal was supposed to be the cause of the histological findings. In contrast to several reports in the literature, however, no indications of an inversion of the teat canal epithelium were found. On the basis of these findings, the hitherto assumed idea of pathogenesis of proliferations of the Fürstenberg's rosette was modified and a new pathogenesis pattern was developed.