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Phaseolotoxin is an antimetabolite toxin produced by diverse pathovars of Pseudomonas syringae which affects various plants, causing diseases of economic importance. Phaseolotoxin contributes to the systemic dissemination of the pathogen in the plant, therefore it is recognized as a major virulence factor. Genetic traits such as the Pht cluster, appear defining to the toxigenic strains phaseolotoxin producers. Extensive research has contributed to our knowledge concerning the regulation of phaseolotoxin revealing a complex regulatory network that involves processes at the transcriptional and posttranscriptional levels, in which specific and global regulators participate. Even more, significant advances in understanding how specific signals, including host metabolites, nutrient sources, and physical parameters such as the temperature, can affect phaseolotoxin production have been made. A general overview of the phaseolotoxin regulation, focusing on the chemical and physical cues, and regulatory pathways involved in the expression of this major virulence factor will be given in the present work.
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Heavy metal pollution is a severe concern worldwide, owing to its harmful effects on ecosystems. Phytoremediation has been applied to remove heavy metals from water, soils, and sediments by using plants and associated microorganisms to restore contaminated sites. The Typha genus is one of the most important genera used in phytoremediation strategies because of its rapid growth rate, high biomass production, and the accumulation of heavy metals in its roots. Plant growth-promoting rhizobacteria have attracted much attention because they exert biochemical activities that improve plant growth, tolerance, and the accumulation of heavy metals in plant tissues. Because of their beneficial effects on plants, some studies have identified bacterial communities associated with the roots of Typha species growing in the presence of heavy metals. This review describes in detail the phytoremediation process and highlights the application of Typha species. Then, it describes bacterial communities associated with roots of Typha growing in natural ecosystems and wetlands contaminated with heavy metals. Data indicated that bacteria from the phylum Proteobacteria are the primary colonizers of the rhizosphere and root-endosphere of Typha species growing in contaminated and non-contaminated environments. Proteobacteria include bacteria that can grow in different environments due to their ability to use various carbon sources. Some bacterial species exert biochemical activities that contribute to plant growth and tolerance to heavy metals and enhance phytoremediation.
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Plant-associated bacteria in heavy-metal-contaminated environments could be a biotechnological tool to improve plant growth. The present work aimed to isolate lead- and cadmium-tolerant endophytic bacteria from the roots of Typha latifolia growing in a site contaminated with these heavy metals. Endophytic bacteria were characterized according to Pb and Cd tolerance, plant-growth-promoting rhizobacteria activities, and their effect on T. latifolia seedlings exposed and non-exposed to Pb and Cd. Pb-tolerant isolates were identified as Pseudomonas azotoformans JEP3, P. fluorescens JEP8, and P. gessardii JEP33, while Cd-tolerant bacteria were identified as P. veronii JEC8, JEC9, and JEC11. They all exert biochemical activities, including indole acetic acid synthesis, siderophore production, and phosphate solubilization. Plant-bacteria interaction assays showed that P. azotoformans JEP3, P. fluorescens JEP8, P. gessardii JEP33, and P. veronii JEC8, JEC9, JEC11 promote the growth of T. latifolia seedlings by increasing the root and shoot length, while in plants exposed to either 5 mg/L of Pb or 10 mg/L of Cd, all bacterial isolates increased the shoot length and the number of roots per plant, suggesting that they are plant-growth-promoting rhizobacteria that could contribute to T. latifolia adaptation to the heavy metal polluted site.
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Phaseolotoxin is a major virulence factor of the bean pathogen bacterium P. savastanoi pv. phaseolicola. This toxin plays a key role in the development of the halo blight disease in bean plants. So far, the signal transduction pathways involved in the synthesis of phaseolotoxin have not been elucidated. The influence of regulation mechanisms related to the oxidative stress response, in particular the OxyR protein, it has been suggested to be involved in this process.. In this study we evaluated the role of OxyR in P. savastanoi pv. phaseolicola, mainly compared to the synthesis of phaseolotoxin and the virulence of this phytopathogen. Generation of the oxyR-mutant, pathogenicity and virulence tests, and analyses of gene expression by RT-PCR assays were performed. The results showed that OxyR exerts an effect on the synthesis of phaseolotoxin and positively influences the expression of the Pht and Pbo cluster genes. Likewise, OxyR influences the production of pyoverdine by the control of the expression of the genes encoding the PvdS sigma factor, involved in the synthesis of this pigment. This study is the first report on members of the OxyR regulon of P. savastanoi pv. phaseolicola NPS3121.
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The Typha genus comprises plant species extensively studied for phytoremediation processes. Recently, Pseudomonas rhodesiae GRC140, an IAA-producing bacterium, was isolated from Typha latifolia roots. This bacterium stimulates the emergence of lateral roots of Arabidopsis thaliana in the presence and absence of cadmium. However, the bacterial influence on cadmium accumulation by the plant has not been determined. Moreover, the P. rhodesiae GRC140 effect in Cd phytoextraction by T. latifolia remains poorly understood. In this work, an axenic hydroponic culture of T. latifolia was established. The plants were used to evaluate the effects of cadmium stress in axenic plants and determine the effects of P. rhodesiae GRC140 and exogenous indole acetic acid (IAA) on Cd tolerance and Cd uptake by T. latifolia. Biomass production, total chlorophyll content, root electrolyte leakage, catalase activity, total glutathione, and Cd content were determined. The results showed that Cd reduces shoot biomass and increases total glutathione and Cd content in a dose-dependent manner in root tissues. Furthermore, P. rhodesiae GRC140 increased Cd translocation to the shoots, while IAA increased the Cd accumulation in plant roots, indicating that both treatments increase Cd removal by T. latifolia plants. These results indicate that axenic plants in hydroponic systems are adequate to evaluate the Cd effects in plants and suggest that T. latifolia phytoextraction abilities could be improved by P. rhodesiae GRC140 and exogenous IAA application.
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Several aflatoxin inhibitors can modulate the antioxidant system in fungi. In this work, the effect of the ethanolic extract of Capsicum chinense and Piper nigrum fruits, capsaicin, and piperine on the expression of the aflE, aflG, aflH, aflI, aflK, aflL, aflO, aflP, and aflQ genes involved in the aflatoxin biosynthetic pathway in Aspergillus parasiticus were studied by qRT-PCR analysis. As well as, the effect on the expression of fungal antioxidant genes (sod1, catA, and cat2) and enzymatic activity of catalase (CAT) and superoxide dismutase (SOD). Results reveal that the highest (p < 0.05) radial growth inhibition (68 and 86%) and aflatoxins production inhibition (73 and 80%) was observed with capsaicin and piperine respectively, at 300 µg/mL, instead of the ethanolic extract at the same concentration. The qRT-PCR analysis showed that compounds and extracts at 300 µg/mL induced a down-regulation of aflatoxin genes and an up-regulation on the fungal antioxidant genes. CAT activity increased by 23.15, 36.65, 51.40, and 65.50%, in the presence of C. chinense and P. nigrum extract, capsaicin, and piperine exposure, respectively. While SOD activity was not significantly impacted (p > 0.05). In conclusion, the capsaicin and piperine, two antifungal and anti-aflatoxigenic compounds produce an up-regulation of antioxidant defense genes accompanied by an enhancement of catalase enzymatic activity in A. parasiticus.
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Aflatoxinas , Capsicum , Piper nigrum , Aflatoxinas/análisis , Alcaloides , Antioxidantes/farmacología , Aspergillus , Benzodioxoles , Capsaicina/farmacología , Catalasa/genética , Frutas/química , Piperidinas , Extractos Vegetales/farmacología , Alcamidas Poliinsaturadas , Superóxido Dismutasa/genéticaRESUMEN
Cadmium (Cd) is one of the most toxic heavy metals for plant physiology and development. This review discusses Cd effects on auxin biosynthesis and homeostasis, and the strategies for restoring plant growth based on exogenous auxin application. First, the two well-characterized auxin biosynthesis pathways in plants are described, as well as the effect of exogenous auxin application on plant growth. Then, review describes the impacts of Cd on the content, biosynthesis, conjugation, and oxidation of endogenous auxins, which are related to a decrease in root development, photosynthesis, and biomass production. Finally, compelling evidence of the beneficial effects of auxin-producing rhizobacteria in plants exposed to Cd is showed, focusing on photosynthesis, oxidative stress, and production of antioxidant compounds and osmolytes that counteract Cd toxicity, favoring plant growth and improve phytoremediation efficiency. Expanding our understanding of the positive effects of exogenous auxins application and the interactions between bacteria and plants growing in Cd-polluted environments will allow us to propose phytoremediation strategies for restoring environments contaminated with this metal.
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Cadmio , Ácidos Indolacéticos , Cadmio/análisis , Ácidos Indolacéticos/metabolismo , Antioxidantes , Biodegradación Ambiental , Plantas/metabolismo , Bacterias/metabolismoRESUMEN
Affinin present in Heliopsis longipes roots has been identified as an anti-aflatoxin molecule. However, its mechanism of action has yet to be clarified. Aflatoxins biosynthesis involves not less than 27 enzymatic reactions. In this work, the genes aflG, aflH, aflI, aflK, aflL, aflM, aflO, aflP, and aflQ of the aflatoxins cluster and the aflS gene encoding an internal regulatory factor involved in aflatoxins biosynthesis in Aspergillus parasiticus, were studied by qRT-PCR. Results demonstrated that ethanolic extract of H. longipes roots and affinin inhibit aflatoxin biosynthesis and fungal growth in a dose-dependent manner. At 300 µg/mL, ethanolic extract and affinin presented the highest inhibition of radial growth (86% and 94%) and aflatoxin production (68% and 80%). The qRT-PCR analysis demonstrated that nine tested genes were down-regulated by affinin and ethanolic extract. The most down-regulated was the aflK, a gene that encodes an enzyme cyclase with double function during the aflatoxin biosynthesis. While no significant down-regulation was obtaining for aflH gene. Exposure to affinin also resulted in decreased transcript levels of the internal regulator factor aflS. Based on our results, a model showing the regulatory mechanism in aflatoxin biosynthesis and its role in gene expression was proposed. In conclusion, affinin modulates the expression of several aflatoxin biosynthetic genes, leading to mycotoxin biosynthesis inhibition. Therefore, H. longipes roots is a suitable candidate to developed control strategies via lowering gene expressions as a future perspective in reducing aflatoxin contamination.
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Aflatoxinas , Aspergillus/genética , Asteraceae/química , Alcamidas Poliinsaturadas , Regulación hacia Abajo , Raíces de PlantasRESUMEN
Introducción: Es poco lo que se conoce sobre aspectos biológicos y poblacionales de Acanthaster planci en México. Su tamaño se ha relacionado con la cantidad de alimento que consume, por lo que es relevante conocer esta variable. Objetivo: Describir la variabilidad en el diámetro del disco de la especie y de su alimentación en cuatro sitios al sur del Golfo de California. Métodos: De febrero 2008 a marzo 2009 se visitaron mensualmente los sitios. Se registró el diámetro del disco de un total de 389 individuos observados. El diámetro del disco se midió con una cinta métrica flexible colocada de lado a lado, en el borde donde comienzan los brazos. Para facilitar la descripción, el diámetro del disco se clasificó en tres grupos: pequeños ( 26 cm). Del total, 298 organismos se encontraron alimentándose. Se clasificaron los tipos de organismos consumidos en corales duros, otros invertebrados y algas. Para calcular la probabilidad de que individuos del mismo tamaño se alimenten de diferente variedad de organismos, se aplicó el índice de Diversidad de Simpson. Con el fin de detectar diferencias del diámetro del disco entre los meses de estudio y los sitios de muestreo, se aplicó un análisis permutacional de varianza. Resultados: Acanthaster planci tuvo un promedio de diámetro del disco de 18.23 ± 0.21 cm, con un mínimo de 7 cm y un máximo de 36 cm. La estructura de tallas fue unimodal y se caracterizó por la predominancia de individuos medianos, seguida por los pequeños y en baja proporción por los grandes. El diámetro del disco fue estadísticamente más grande en febrero y más pequeño en los últimos meses del año. En San Rafaelito se encontraron los ejemplares más grandes y en Ensenada de Muertos los más pequeños. Los individuos de tamaño mediano fueron más frecuentes a lo largo del año y en todos los sitios. Los individuos pequeños se alimentaron significativamente más de Porites panamensis, seguido de algas coralinas y tapetes. Los más grandes tuvieron los valores de diversidad más bajos, más del 90 % de los individuos en este grupo se alimentaron de Pocillopora spp. y algunos de macroalgas. Los medianos presentaron los valores más altos de diversidad y consumieron todos los tipos de alimento encontrados, sin embargo, como era de esperarse, muchos fueron selectivos hacia Pocillopora spp. Conclusiones: Acanthaster planci se considera un individuo de tamaño mediano en el área de estudio. Se detectó una evidente relación entre el diámetro del disco y la diversidad del tipo de alimento. Los tres grupos de tallas fueron positivamente selectivos hacia corales duros; los más pequeños se alimentaron preferentemente de Porites panamensis y en conjunto con los grandes, presentaron valores bajos de diversidad en el tipo de alimento, mientras que los medianos fueron los que consumieron una mayor diversidad de presas.
Introduction: Little is known about the biology and population dynamics of Acanthaster planci in Mexico. Its size is considered relevant because it is related to consumption rate. Objective: To describe the variability of the disk diameter and food type of the species in four sampling sites in the southern Gulf of California. Methods: Each site was visited on a monthly basis from February 2008 to March 2009. Disk diameter was registered from a total of 389 observed specimens. Measurements included only the main disk from side to side where the arms begin. A flexible tape was used to measure disk diameter. Three size groups were created for easier explanations: small ( 26 cm). From the total, only 298 asteroids were feeding. Preyed organisms were classified in hard corals, other invertebrates, and algae. The Simpson's Diversity Index was used to calculate the probability that any specimen would be feeding on different types of organisms. A permutational analysis of variance was used to detect differences of disk diameter amongst months and study sites. Results: This population of Acanthaster planci had an average disk diameter = 18.23 ± 0.21 cm, with a minimum of 7 cm and maximum of 36 cm. Size structure was unimodal and largely dominated by medium sized specimens, followed by small ones. The larger were less frequent. Disk diameter was statistically higher in February and lower in the last months of the year. San Rafaelito was the site with the largest and Ensenada de Muertos with the smallest organisms. Medium sized individuals were dominant throughout the year and sampling sites. The small sized fed significantly more on Porites panamensis and coralline algae and turf. Larger specimens had the lowest diversity values, more than 90 % of this size group fed on Pocillopora spp. and very few on macroalgae. Medium sized specimens showed the highest diversity, feeding on all the food types, although as expected, many of them were selective to Pocillopora spp. Conclusions: Acanthaster planci can be considered a medium sized species in the study area. There is an evident food selectivity regarding disk diameter size. The three size groups were positively selective to hard corals, but only the small favored Porites panamensis; and together with large individuals, they had the lowest diversity on food types, whereas medium sized asteroids fed on a high diversity of prey.
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Animales , Estrellas de Mar/crecimiento & desarrollo , Equinodermos/crecimiento & desarrollo , MéxicoRESUMEN
In the present study, α-amylase and α-glucosidase inhibitory effect and antioxidant activity of capsaicin and piperine from the ethanolic extract of Capsicum chinense (EECch) and Piper nigrum (EEPn) fruits were investigated. Results revealed that EECch exhibited the highest phenolic (154 mg GAE/100 g of tissue) and flavonoid content (75 mg RtE/100 g of tissue) in comparison with EEPn. The predominant compound detected in EECch and EEPn by GC-EIMS analysis was the capsaicin and piperine, respectively. The capsaicin and piperine showed the highest α-amylase and α-glucosidase inhibitory effect and antioxidant activity rather than extracts. The EEPn (IC50= 216 µg/mL) and piperine (IC50= 105 µg/mL) present a highest α-amylase inhibitory effect, while the EECch (IC50= 225 µg/mL) and capsaicin (IC50= 117 µg/mL) showed highest anti-α-glucosidase activity. Molecular docking established that capsaicin and piperine bind at the α-glucosidase and α-amylase through hydrophobic interactions, hydrogen bond, and charge interactions with amino acid residues. The enzyme inhibitory activity and antioxidant properties exhibited by EECch and EEPn could be attributed to the capsaicin and piperine content and other compounds present such as phenolic compounds and flavonoids. These fruits are potential sources of natural antioxidant agents and α-amylase and α-glucosidase inhibitors.
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Alcaloides/farmacología , Benzodioxoles/farmacología , Capsaicina/farmacología , Capsicum/química , Inhibidores Enzimáticos/farmacología , Piper nigrum/química , Piperidinas/farmacología , Alcamidas Poliinsaturadas/farmacología , alfa-Amilasas/antagonistas & inhibidores , Frutas/química , Inhibidores de Glicósido Hidrolasas/farmacología , Extractos Vegetales/químicaRESUMEN
In this work, we isolated four Cd-tolerant endophytic bacteria from Typha latifolia roots that grow at a Cd-contaminated site. Bacterial isolates GRC065, GRC066, GRC093, and GRC140 were identified as Pseudomonas rhodesiae. These bacterial isolates tolerate cadmium and have abilities for phosphate solubilization, siderophore production, indole acetic acid (IAA) synthesis, and ACC deaminase activity, suggesting that they are plant growth-promoting rhizobacteria. Bacterial inoculation in Arabidopsis thaliana seedlings showed that P. rhodesiae strains increase total fresh weight and number of lateral roots concerning non-inoculated plants. These results indicated that P. rhodesiae strains promote A. thaliana seedlings growth by modifying the root system. On the other hand, in A. thaliana seedlings exposed to 2.5 mg/l of Cd, P. rhodesiae strains increased the number and density of lateral roots concerning non-inoculated plants, indicating that they modify the root architecture of A. thaliana seedlings exposed to cadmium. The results showed that P. rhodesiae strains promote the development of lateral roots in A. thaliana seedlings cultivated in both conditions, with and without cadmium. These results suggest that P. rhodesiae strains could exert a similar role inside the roots of T. latifolia that grow in the Cd-contaminated environment.
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Arabidopsis/microbiología , Cadmio/metabolismo , Cadmio/farmacología , Raíces de Plantas/microbiología , Pseudomonas/genética , Pseudomonas/metabolismo , Biodegradación Ambiental , Endófitos/genética , Endófitos/metabolismo , Fosfatos/metabolismo , Desarrollo de la Planta , Raíces de Plantas/efectos de los fármacos , Pseudomonas/efectos de los fármacos , Pseudomonas/aislamiento & purificación , Plantones/efectos de los fármacos , Plantones/microbiología , Contaminantes del Suelo , SolubilidadRESUMEN
Aflatoxins produced by Aspergillus parasiticus are toxic and carcinogenic metabolites. The biosynthesis of this mycotoxins is a complex process and involves at least 30 genes clustered within an approximately 82 kB gene cluster. In the present study, the effect of Capsicum chinense and Piper nigrum fruits on Aspergillus parasiticus growth and aflatoxin production were studied in relation to the expression of aflD, aflM, aflR, and aflS four; key genes of aflatoxins biosynthesis pathway. GC-EIMS analysis identified capsaicin (66,107 µg g-1) and piperine (1,138 µg g-1) as the most abundant compounds in C. chinense and P. nigrum fruits, respectively. The antifungal and anti-aflatoxigenic assays showed that C. chinense, P. nigrum, capsaicin, and piperine inhibited A. parasiticus growth and aflatoxins production in a dose-dependent manner. The piperine at 300 µg mL-1 produced higher radial growth inhibition (89%) and aflatoxin production inhibition (69%). The expression of aflatoxin biosynthetic genes was evaluated by quantitative real-time PCR (qRT-PCR) and revealed that aflatoxin inhibition occurring via downregulating the aflS and aflR, and subsequently aflD and aflM genes. These results will improve our understanding of the mechanism of aflatoxin regulation by C. chinense, P. nigrum, capsaicin, and piperine, and provides a reference for further study.
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Aflatoxinas/metabolismo , Aspergillus/efectos de los fármacos , Capsicum/química , Regulación Fúngica de la Expresión Génica/efectos de los fármacos , Piper nigrum/química , Aflatoxinas/genética , Alcaloides/farmacología , Antifúngicos/química , Antifúngicos/farmacología , Aspergillus/genética , Aspergillus/crecimiento & desarrollo , Aspergillus/metabolismo , Benzodioxoles/farmacología , Vías Biosintéticas , Capsaicina/farmacocinética , Proteínas de Unión al ADN/genética , Frutas/química , Proteínas Fúngicas/genética , Genes Fúngicos , Piperidinas/farmacología , Alcamidas Poliinsaturadas/farmacología , Factores de Transcripción/genéticaRESUMEN
In the present study, ethanolic extract from Heliopsis longipes roots and affinin/spilanthol against Aspergillus parasiticus growth and aflatoxins production were studied in relation to the expression of aflD and aflR, two key genes of aflatoxins biosynthetic pathway. Phytochemical analysis of the ethanolic extract by GC-EIMS identified affinin/spilanthol (7.84 ± 0.27 mg g-1) as the most abundant compounds in H. longipes roots. The antifungal and anti-aflatoxigenic assays showed that affinin/spilanthol at 300 µg mL-1 produced the higher inhibition of radial growth (95%), as well as, the higher aflatoxins production inhibition (61%) in comparison to H. longipes roots (87% and 48%, respectively). qRT-PCR revealed that the expression of aflD and aflR genes showed a higher downregulation in affinin/spilanthol at 300 µg mL-1. The expression ratio of alfD was suppressed by affinin/spilanthol in 79% and aflR in 84%, while, a lower expression ratio suppressed by H. longipes was obtained, alfD (55%) and aflR (59%). Affinin/spilanthol possesses higher antifungal and anti-aflatoxigenic activity against A. parasiticus rather than H. longipes roots, and this anti-aflaxotigenic activity occurring via downregulation of the aflD and aflR genes. Thus, H. longipes roots and affinin/spilanthol can be considered potent antifungal agents against aflatoxigenic fungus, especially, affinin/spilanthol.
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Antifúngicos/farmacología , Aspergillus/efectos de los fármacos , Asteraceae/química , Extractos Vegetales/farmacología , Alcamidas Poliinsaturadas/farmacología , Aflatoxinas/biosíntesis , Aflatoxinas/genética , Antifúngicos/química , Aspergillus/genética , Aspergillus/metabolismo , Vías Biosintéticas , Proteínas de Unión al ADN/genética , Regulación hacia Abajo , Proteínas Fúngicas/genética , Regulación Fúngica de la Expresión Génica/efectos de los fármacos , Extractos Vegetales/química , Raíces de Plantas/química , Alcamidas Poliinsaturadas/análisis , Factores de Transcripción/genéticaRESUMEN
Bacillus sp. strain MA04 a plant growth-promoting rhizobacteria (PGPR) showed hemolytic activity on blood agar plates, and the supernatant from liquid culture in nutrient broth at 24 h exhibited emulsification activity, suggesting the production of biosurfactants. In antagonist assays, the supernatant showed antifungal activity against phytopathogenic fungi such as Penicillium expansum, Fusarium stilboides, Sclerotium rolfsii y Rhizoctonia solani, finding a reduction of mycelial growth of all fungi tested, ranging from 35 to 69%, this activity was increased with time of culture, accomplishing percentages of inhibition up to 85% with supernatants obtained at 72 h. Then, the crude biorsurfactant (CB) was isolated from the supernatant in order to assay its antagonistic effect on the phytopathogens previously tested, finding an increase in the inhibition up to 97% at 500 mg/L of CB. The composition of CB was determined by infrared spectroscopy, identifying various functional groups related to lipopeptides, which were purified by high-performance liquid chromatography and analyzed by MALDI-TOF/TOF-MS, revealing a mixture of fengycins A and B whose high antifungal activity is been widely recognized. These results show that PGPR Bacillus sp. MA04 could also contribute to plant health status through the production of metabolites with antimicrobial activity.
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Pseudomonas syringae pv. phaseolicola is a phytopathogenic bacterium in beans that produces a phytotoxin called phaseolotoxin, in whose synthesis a group of genes that belong to the "Pht cluster" are involved. This cluster comprises 23 genes arranged in 5 transcriptional units, two monocistronic (argK, phtL) and three polycistronic (phtA, phtD, phtM) operons, whose expression is increased at 18°C, correlating with the production of phaseolotoxin by the bacterium. So far, the regulatory mechanisms involved in phaseolotoxin synthesis are poorly understood and only the requirement of low temperatures for its synthesis has been demonstrated. Therefore, in this study we searched for regulatory proteins that could be involved in the phaseolotoxin synthesis, focusing on the regulation of the phtM operon. Gel shift assays showed that the promoter region of the phtM operon contains binding sites for putative regulatory proteins, which are encoded outside the Pht cluster and are independent of the GacS-GacA two-component system. Deletion assays with the promoter region of the phtM operon show that the binding site for a putative transcription factor is located within a 58bp region. The putative transcription factor of the phtM operon has an apparent molecular mass in the 14-20kDa range. Furthermore, the results demonstrate that the transcription factor recognizes and binds the upstream phtM region as monomer o multimer of a single polypeptide. Our findings provide new insights into the regulatory mechanisms involved in phaseolotoxin production, and suggest that the Pht cluster was integrated into the global regulatory mechanism of P. syringae pv. phaseolicola.
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Operón , Ornitina/análogos & derivados , Pseudomonas syringae , Ornitina/genética , Ornitina/metabolismo , Pseudomonas syringae/genéticaRESUMEN
ETHNOPHARMACOLOGICAL RELEVANCE: Bidens odorata Cav (Asteraceae) is used for the empirical treatment of inflammation and pain. AIM OF THE STUDY: This work evaluated the in vitro and in vivo toxicity, antioxidant activity, as well as the anti-inflammatory and antinociceptive effects of an ethanol extract from Bidens odorata leaves (BOE). MATERIALS AND METHODS: The in vitro toxicity of BOE (10-1000µg/ml) was evaluated with the comet assay in PBMC. The in vivo acute toxicity of BOE (500-5000mg/kg) and the effect of BOE (10-1000µg/ml) on the level of ROS in PBMC were determined. The in vivo anti-inflammatory activity of BOE was assessed using the TPA-induced ear edema in mice. The antinociceptive activities of BOE (50-200mg/kg p.o.) were assessed using the acetic acid and formalin tests. The antinociceptive mechanism of BOE was determined using naloxone and glibenclamide. RESULTS: BOE lacked DNA damage, and showed low in vivo toxicity (LD50 > 5000mg/kg p.o.). BOE inhibited ROS production (IC50 = 252.13 ± 20.54µg/ml), and decreased inflammation by 36.1 ± 3.66%. In both antinociceptive test, BOE (200mg/kg) exerted activity with similar activity than the reference drugs. CONCLUSION: B. odorata exerts low in vitro and in vivo toxicity, antioxidant effects, moderate in vivo anti-inflammatory activity, and antinociceptive effects mediated by ATP-sensitive K+ channels.
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Analgésicos/farmacología , Antioxidantes/farmacología , Bidens/química , Extractos Vegetales/farmacología , Analgésicos/administración & dosificación , Analgésicos/aislamiento & purificación , Animales , Antiinflamatorios/administración & dosificación , Antiinflamatorios/aislamiento & purificación , Antiinflamatorios/farmacología , Antioxidantes/administración & dosificación , Antioxidantes/aislamiento & purificación , Ensayo Cometa , Relación Dosis-Respuesta a Droga , Edema/tratamiento farmacológico , Etanol/química , Concentración 50 Inhibidora , Canales KATP/metabolismo , Dosificación Letal Mediana , Leucocitos Mononucleares/efectos de los fármacos , Leucocitos Mononucleares/metabolismo , Masculino , Ratones , Ratones Endogámicos BALB C , Dolor/tratamiento farmacológico , Extractos Vegetales/administración & dosificación , Extractos Vegetales/toxicidad , Hojas de la Planta , Pruebas de Toxicidad AgudaRESUMEN
Las cactáceas son la vegetación característica de las zonas áridas en México, donde las lluvias son escasas, la evapotranspiración es elevada y la fertilidad de los suelos es baja. Las plantas han desarrollado estrategias fisiológicas como la asociación con microorganismos en la zona de la rizósfera para incrementar la captación de nutrientes. En el presente trabajo se obtuvieron 4 aislados bacterianos de la rizósfera de Mammillaria magnimamma y Coryphantha radians, los que fueron nombrados como QAP3, QAP19, QAP22 y QAP24 e identificados genéticamente como pertenecientes al género Bacillus. Estos aislados exhibieron in vitro propiedades bioquímicas como solubilización de fosfatos, producción de ácido indolacético y actividad ACC deaminasa, que se relacionan con la promoción del crecimiento de las plantas. Dicha promoción fue ensayada inoculando semillas de M. magnimamma y evaluando luego algunos parámetros. Se encontró que todos los aislados incrementaron la germinación desde un 17% hasta un 34,3% (con respecto a las semillas testigo sin inocular); el aislado QAP24 fue el que presentó el mayor efecto en este sentido y permitió la germinación de todas las semillas viables (84,7%) 3 días antes que en el testigo. La inoculación de este aislado en plantas de Mammillaria zeilmanniana mostró un efecto positivo sobre la floración: en 2 meses dentro del período de un año se detectó un incremento en el número de plantas en floración con respecto a las plantas testigo, de hasta el 31,0% en uno de ellos. Se concluye que los aislados de Bacillus spp. caracterizados poseen potencial para ser empleados en programas de conservación de especies vegetales de zonas áridas.
Cacti are the most representative vegetation of arid zones in Mexico where rainfall is scarce, evapotranspiration is high and soil fertility is low. Plants have developed physiological strategies such as the association with microorganisms in the rhizosphere zone to increase nutrient uptake. In the present work, four bacterial isolates from the rhizosphere of Mammillaria magnimamma and Coryphantha radians were obtained and named as QAP3, QAP19, QAP22 and QAP24, and were genetically identified as belonging to the genus Bacillus, exhibiting in vitro biochemical properties such as phosphate solubilization, indoleacetic acid production and ACC deaminase activity related to plant growth promotion, which was tested by inoculating M. magnimamma seeds. It was found that all isolates increased germination from 17 to 34.3% with respect to the uninoculated control seeds, being QAP24 the one having the greatest effect, accomplishing the germination of viable seeds (84.7%) three days before the control seeds. Subsequently, the inoculation of Mammillari zeilmanniana plants with this isolate showed a positive effect on bloom, registering during two months from a one year period, an increase of up to 31.0% in the number of flowering plants compared to control plants. The characterized Bacillus spp. isolates have potential to be used in conservation programs of plant species from arid zones.
Asunto(s)
Bacillus/aislamiento & purificación , Bacillus/clasificación , Adaptación Biológica/fisiología , Conservación de los Recursos Naturales/métodos , Cactaceae/microbiología , Rizosfera , Inoculantes Agrícolas/crecimiento & desarrollo , Germinación/efectos de los fármacos , Flores/efectos de los fármacos , Estándares de Referencia/métodosRESUMEN
Cacti are the most representative vegetation of arid zones in Mexico where rainfall is scarce, evapotranspiration is high and soil fertility is low. Plants have developed physiological strategies such as the association with microorganisms in the rhizosphere zone to increase nutrient uptake. In the present work, four bacterial isolates from the rhizosphere of Mammillaria magnimamma and Coryphantha radians were obtained and named as QAP3, QAP19, QAP22 and QAP24, and were genetically identified as belonging to the genus Bacillus, exhibiting in vitro biochemical properties such as phosphate solubilization, indoleacetic acid production and ACC deaminase activity related to plant growth promotion, which was tested by inoculating M. magnimamma seeds. It was found that all isolates increased germination from 17 to 34.3% with respect to the uninoculated control seeds, being QAP24 the one having the greatest effect, accomplishing the germination of viable seeds (84.7%) three days before the control seeds. Subsequently, the inoculation of Mammillari zeilmanniana plants with this isolate showed a positive effect on bloom, registering during two months from a one year period, an increase of up to 31.0% in the number of flowering plants compared to control plants. The characterized Bacillus spp. isolates have potential to be used in conservation programs of plant species from arid zones.
Asunto(s)
Inoculantes Agrícolas , Bacillus/aislamiento & purificación , Cactaceae/microbiología , Rizosfera , Microbiología del Suelo , Bacillus/clasificación , Bacillus/fisiología , Cactaceae/crecimiento & desarrollo , Flores/crecimiento & desarrollo , Germinación , Raíces de Plantas/microbiología , ARN Bacteriano/genética , ARN Ribosómico 16S/genética , Ribotipificación , Semillas/microbiología , Semillas/fisiología , SimbiosisRESUMEN
ETHNOPHARMACOLOGICAL RELEVANCE: Costus pulverulentus C. Presl (Costaceae), a species endemic to Mexico, is used for the empirical treatment of cancer, pain, and inflammation. AIM OF THE STUDY: The objective of this study was to evaluate the toxicity, as well as the cytotoxic, antinociceptive, anti-inflammatory and sedative effects of an ethanol extract from Costus pulverulentus stem (CPE). MATERIALS AND METHODS: The chemical characterization of CPE was performed by Gas chromatography-mass spectrometry (GC-MS). The toxicity of CPE was evaluated using the comet assay (10-1000 µg/ml during 5h) and the acute toxicity test (500-5000 mg/kg p.o. and i.p. during 14 days). The cytotoxic effect of CPE (1-250 µg/ml) on human cancer cells was evaluated using the MTT assay. The antinociceptive effects of CPE (50-200mg/kg p.o.) were evaluated using thermal-induced nociception tests (hot plate and tail flick) and the chemical-induced nociceptive tests (acetic acid and formalin). The sedative activity of CPE (50-200mg/kg p.o.) was evaluated using the ketamine-induced sleeping time test. RESULTS: CPE showed the presence of compounds such as campesterol, stigmasterol ß-sitosterol, vanillic acid, among others. In the comet assay, CPE at 200 µg/ml or higher concentrations induced DNA damage. In the acute toxicity test, the LD50 estimated for CPE was>5000 mg/kg p.o. or i.p. CEP showed moderate cytotoxic effects on prostate carcinoma cells PC-3 cells (IC50=179 ± 23.2 µg/ml). In the chemical-induced nociception models, CPE (100 and 200mg/kg p.o.) showed antinociceptive effects with similar activity to 100mg/kg naproxen. In the thermal-induced nociception tests, CPE tested at 200mg/kg showed moderate antinociceptive effects by 28% (hot plate test) and by 25% (tail flick test). In the ketamine-induced sleeping time test, CPE showed no sedative effects. CONCLUSIONS: C. pulverulents exerts moderate cytotoxic effects in human cancer cells, moderate anti-inflammatory and antinociceptive effects. C. pulverulentus induces antinociceptive effects without inducing sedation.
Asunto(s)
Analgésicos/farmacología , Antiinflamatorios/farmacología , Costus , Extractos Vegetales/farmacología , Ácido Acético , Analgésicos/uso terapéutico , Analgésicos/toxicidad , Anestésicos Disociativos/farmacología , Animales , Antiinflamatorios/uso terapéutico , Antiinflamatorios/toxicidad , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Ensayo Cometa , Edema/tratamiento farmacológico , Formaldehído , Calor , Humanos , Ketamina/farmacología , Dosificación Letal Mediana , Leucocitos Mononucleares/efectos de los fármacos , Leucocitos Mononucleares/metabolismo , Masculino , Ratones Endogámicos BALB C , Dolor/inducido químicamente , Dolor/tratamiento farmacológico , Extractos Vegetales/uso terapéutico , Extractos Vegetales/toxicidad , Tallos de la PlantaRESUMEN
Abstract Despite the important effort of knowing the Ophiuroidea diversity in the Mexican Pacific, some mistakes in the taxonomic nomenclature have pervaded through time. In order to clarify the latter, a checklist based on literature review of brittle stars from the Mexican Pacific is provided. We reviewed a total of 105 references that in total summarized 125 species of brittle stars from the Mexican Pacific (112) and the Gulf of California (97), belonging to two orders, 16 families and 50 genera. These records are higher than those reported on previous studies carried out in the area. México is the country with the highest number of brittle stars reported in the Tropical Eastern Pacific; this may be due to its exceptional oceanographic conditions, location and coastline extension. However, a total of 27 species reported in the literature were considered doubtful due to their general distributions and were not included in the checklist. Of the reported species, 35 have their type locality in the Mexican Pacific, corroborating the importance of the country in the Tropical Eastern Pacific in terms of Echinodermata diversity. Rev. Biol. Trop. 63 (Suppl. 2): 37-47. Epub 2015 June 01.
Resumen A pesar del importante esfuerzo en el conocimiento de la diversidad de ofiuroideos del Pacífico mexicano, algunos errores en la nomenclatura taxonómica han perseverado a través del tiempo. Con el objetivo de clarificar lo anterior, se provee una lista de especies de ofiuroideos del Pacífico Mexicano, basada en la revisión de literatura. Se revisó un total de 105 referencias que en total suman 125 especies pertenecientes a dos órdenes, 16 familias y 50 géneros de ofiuros del Pacífico Mexicano (112) y el Golfo de California (97). Estos reportes son mayores que otros estudios llevados a cabo en el área. México es el país con el mayor número de ofiuros reportados en el Pacífico Oriental Tropical; esto puede deberse a su excepcional condición oceanográfica, ubicación y extensión de línea de costa. Un total de 27 de las especies reportadas en la literatura fueron consideradas dudosas de acuerdo distribución geográfica por lo que no fueron incluidas en la lista. 35 especies poseen su localidad tipo en el Pacífico Mexicano, corroborando la importancia de México en el Pacífico Oriental Tropical en términos de diversidad de equinodermos.